Enterococcus phage phiFL1A

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Phifelvirus; Enterococcus virus FL1

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 61 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D2IYV1|D2IYV1_9CAUD Uncharacterized protein gp29 OS=Enterococcus phage phiFL1A OX=673832 GN=gp29 PE=4 SV=1
MM1 pKa = 7.72GEE3 pKa = 3.9IAEE6 pKa = 4.25MMLEE10 pKa = 4.23GVLCASCGVFLDD22 pKa = 3.84VDD24 pKa = 3.93GNGYY28 pKa = 9.46PEE30 pKa = 4.14YY31 pKa = 11.06CEE33 pKa = 5.4DD34 pKa = 3.74CQEE37 pKa = 4.13QIIEE41 pKa = 4.03EE42 pKa = 4.18DD43 pKa = 3.31HH44 pKa = 6.38RR45 pKa = 5.97

Molecular weight: 5.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D2IYU5|D2IYU5_9CAUD Uncharacterized protein gp23 OS=Enterococcus phage phiFL1A OX=673832 GN=gp23 PE=4 SV=1
MM1 pKa = 7.71GKK3 pKa = 7.96TKK5 pKa = 10.86SKK7 pKa = 10.16IKK9 pKa = 9.94KK10 pKa = 8.47KK11 pKa = 10.21KK12 pKa = 9.61RR13 pKa = 11.84RR14 pKa = 11.84LKK16 pKa = 10.48EE17 pKa = 3.45KK18 pKa = 10.44AIANGTYY25 pKa = 10.09SKK27 pKa = 10.47RR28 pKa = 11.84GKK30 pKa = 10.12SDD32 pKa = 2.59GMYY35 pKa = 10.88KK36 pKa = 8.91MQGTDD41 pKa = 2.94DD42 pKa = 4.13CLGKK46 pKa = 10.45RR47 pKa = 3.97

Molecular weight: 5.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

61

0

61

11623

33

1304

190.5

21.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.427 ± 0.536

0.74 ± 0.119

5.928 ± 0.384

7.717 ± 0.624

3.906 ± 0.269

6.169 ± 0.368

1.093 ± 0.136

7.158 ± 0.303

8.896 ± 0.516

8.346 ± 0.311

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.65 ± 0.289

6.642 ± 0.386

2.693 ± 0.197

4.156 ± 0.251

3.88 ± 0.411

6.479 ± 0.617

5.954 ± 0.4

6.066 ± 0.219

1.144 ± 0.128

3.958 ± 0.45
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski