Nitratifractor salsuginis (strain DSM 16511 / JCM 12458 / E9I37-1)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Epsilonproteobacteria; Campylobacterales; Sulfurovaceae; Nitratifractor; Nitratifractor salsuginis

Average proteome isoelectric point is 6.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2076 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E6X004|E6X004_NITSE Histidine triad (HIT) protein OS=Nitratifractor salsuginis (strain DSM 16511 / JCM 12458 / E9I37-1) OX=749222 GN=Nitsa_1479 PE=4 SV=1
MM1 pKa = 7.22YY2 pKa = 10.12LLKK5 pKa = 10.67GALYY9 pKa = 10.38RR10 pKa = 11.84LFISGVIVLLSAISAQADD28 pKa = 2.86ISGIVFRR35 pKa = 11.84DD36 pKa = 3.87FNLNGVKK43 pKa = 9.36DD44 pKa = 3.79TLEE47 pKa = 4.37PGVAGLTVAAYY58 pKa = 9.81DD59 pKa = 4.34DD60 pKa = 4.04SGQAVDD66 pKa = 3.87TVMTNSDD73 pKa = 3.55GNYY76 pKa = 9.53TLATDD81 pKa = 4.22PGTYY85 pKa = 8.91RR86 pKa = 11.84VEE88 pKa = 4.28VSGVPAFLKK97 pKa = 10.57PGTSLSGNTRR107 pKa = 11.84PLVDD111 pKa = 3.9RR112 pKa = 11.84VTDD115 pKa = 4.06GATHH119 pKa = 5.88NVGLISAGEE128 pKa = 4.19YY129 pKa = 9.75CQSNPDD135 pKa = 3.27ILVTRR140 pKa = 11.84FTKK143 pKa = 10.58YY144 pKa = 10.64NRR146 pKa = 11.84DD147 pKa = 3.18GSNGQISVFLEE158 pKa = 3.73YY159 pKa = 10.35AYY161 pKa = 9.25TANTTDD167 pKa = 5.34DD168 pKa = 5.23DD169 pKa = 4.25STDD172 pKa = 3.52DD173 pKa = 4.03PKK175 pKa = 11.67AEE177 pKa = 4.51LGTYY181 pKa = 8.11SQIGSIYY188 pKa = 10.2GLAHH192 pKa = 6.49FKK194 pKa = 9.92EE195 pKa = 4.41ANITYY200 pKa = 10.22LGTYY204 pKa = 9.45LKK206 pKa = 10.12RR207 pKa = 11.84HH208 pKa = 6.16ADD210 pKa = 3.27IGPSGMGAIYY220 pKa = 10.01KK221 pKa = 9.65YY222 pKa = 10.62DD223 pKa = 3.63HH224 pKa = 7.14ASGTFSTLITLPTYY238 pKa = 10.46DD239 pKa = 4.05PRR241 pKa = 11.84NGAGQGYY248 pKa = 9.55DD249 pKa = 3.2WDD251 pKa = 4.89HH252 pKa = 6.6DD253 pKa = 3.94TEE255 pKa = 4.4AYY257 pKa = 9.86RR258 pKa = 11.84WVGKK262 pKa = 8.67TGIGDD267 pKa = 3.56IEE269 pKa = 4.19ISEE272 pKa = 4.19DD273 pKa = 3.56EE274 pKa = 4.11QRR276 pKa = 11.84LFAVDD281 pKa = 4.35LYY283 pKa = 11.33DD284 pKa = 4.04RR285 pKa = 11.84KK286 pKa = 10.91LYY288 pKa = 11.04VIDD291 pKa = 5.0LNQSGDD297 pKa = 3.69ATGHH301 pKa = 5.59TGYY304 pKa = 10.59DD305 pKa = 3.13IPNPCGNDD313 pKa = 2.83TDD315 pKa = 4.45FRR317 pKa = 11.84PMALGYY323 pKa = 9.99RR324 pKa = 11.84DD325 pKa = 3.14GKK327 pKa = 10.84LYY329 pKa = 11.24VGVTCTAEE337 pKa = 4.19STVDD341 pKa = 4.09PDD343 pKa = 4.2NQDD346 pKa = 3.5DD347 pKa = 4.17STNGPRR353 pKa = 11.84KK354 pKa = 9.59GDD356 pKa = 3.57RR357 pKa = 11.84NALSAHH363 pKa = 7.53IYY365 pKa = 10.56RR366 pKa = 11.84FDD368 pKa = 4.65PDD370 pKa = 3.64STSFEE375 pKa = 4.61PNAVLDD381 pKa = 3.69INLTYY386 pKa = 10.91DD387 pKa = 3.55RR388 pKa = 11.84GCIHH392 pKa = 7.03SSDD395 pKa = 4.58VSNQDD400 pKa = 3.17PSTCTYY406 pKa = 10.85QDD408 pKa = 3.46WDD410 pKa = 4.47DD411 pKa = 3.4QTQPYY416 pKa = 8.46RR417 pKa = 11.84ANWNPWQMDD426 pKa = 3.41YY427 pKa = 11.37DD428 pKa = 3.73IVFNDD433 pKa = 4.72KK434 pKa = 11.01KK435 pKa = 10.7PGDD438 pKa = 4.39IGNQDD443 pKa = 2.52NWIEE447 pKa = 3.91YY448 pKa = 7.25MQPLLSDD455 pKa = 3.39IVFDD459 pKa = 4.48NDD461 pKa = 2.78GSMIIQIRR469 pKa = 11.84DD470 pKa = 3.32VNGDD474 pKa = 2.87RR475 pKa = 11.84GGYY478 pKa = 9.62KK479 pKa = 10.16NYY481 pKa = 10.58SPNPNDD487 pKa = 3.42TTRR490 pKa = 11.84QNMNGEE496 pKa = 4.01GDD498 pKa = 3.4ILRR501 pKa = 11.84ACGNPQSGWTLEE513 pKa = 4.09NNGEE517 pKa = 4.27CGGVQTNGANNHH529 pKa = 6.0EE530 pKa = 4.53GPGGGEE536 pKa = 4.41YY537 pKa = 10.53YY538 pKa = 10.19WNDD541 pKa = 3.26QGPGGTNHH549 pKa = 5.51STAGSAYY556 pKa = 10.27QGDD559 pKa = 4.42AGHH562 pKa = 6.95SDD564 pKa = 3.15TSMGGLLVVPGYY576 pKa = 9.84PDD578 pKa = 3.5VVTGLMDD585 pKa = 3.3VTDD588 pKa = 4.05YY589 pKa = 11.71LNNGLGWLRR598 pKa = 11.84NDD600 pKa = 3.43TGEE603 pKa = 4.09LAKK606 pKa = 10.92DD607 pKa = 3.57GSGNPKK613 pKa = 9.9RR614 pKa = 11.84LLVSEE619 pKa = 4.91DD620 pKa = 4.14DD621 pKa = 3.29QDD623 pKa = 3.41KK624 pKa = 10.89FYY626 pKa = 11.42GKK628 pKa = 10.8GSGIGDD634 pKa = 4.4LEE636 pKa = 4.32ALCDD640 pKa = 3.73PAPIEE645 pKa = 3.83IGNYY649 pKa = 6.71VWEE652 pKa = 5.27DD653 pKa = 3.17SDD655 pKa = 4.92GDD657 pKa = 4.33GIQDD661 pKa = 3.79PGEE664 pKa = 4.1SPIANVTVKK673 pKa = 10.71LYY675 pKa = 10.5DD676 pKa = 3.59ANGQEE681 pKa = 4.44IGSTTTDD688 pKa = 2.99SNGEE692 pKa = 4.23YY693 pKa = 10.76YY694 pKa = 10.77FGGASNARR702 pKa = 11.84GVALAPHH709 pKa = 5.8THH711 pKa = 4.57YY712 pKa = 10.76QIRR715 pKa = 11.84IALNDD720 pKa = 3.05QHH722 pKa = 8.06LNGRR726 pKa = 11.84VPTTRR731 pKa = 11.84DD732 pKa = 3.58ANPSAPDD739 pKa = 3.53SDD741 pKa = 4.49DD742 pKa = 3.44QHH744 pKa = 8.9DD745 pKa = 3.84SDD747 pKa = 5.38GDD749 pKa = 3.76NGTLNPGFSTIDD761 pKa = 3.45YY762 pKa = 6.87TTGGAGKK769 pKa = 9.69NDD771 pKa = 3.2HH772 pKa = 6.42TLDD775 pKa = 3.68FGFVPPSSLGDD786 pKa = 3.89YY787 pKa = 10.52VWVDD791 pKa = 3.42SNRR794 pKa = 11.84DD795 pKa = 3.43GIQNDD800 pKa = 3.96GANAGIQGVTVHH812 pKa = 6.95LYY814 pKa = 10.81KK815 pKa = 10.85DD816 pKa = 3.7GQDD819 pKa = 3.25TGRR822 pKa = 11.84TATTDD827 pKa = 3.2GNGKK831 pKa = 9.28YY832 pKa = 10.46LFDD835 pKa = 3.86NLVPGSYY842 pKa = 9.48HH843 pKa = 6.8VKK845 pKa = 9.65FDD847 pKa = 3.55VPNGYY852 pKa = 9.3VVSPKK857 pKa = 10.66DD858 pKa = 3.24QGNDD862 pKa = 3.06EE863 pKa = 4.76TKK865 pKa = 11.02DD866 pKa = 3.46SDD868 pKa = 3.94ADD870 pKa = 3.76PATGATVDD878 pKa = 3.55TDD880 pKa = 4.04LSAGEE885 pKa = 5.22NDD887 pKa = 4.02LTWDD891 pKa = 3.23MGIYY895 pKa = 9.77PVADD899 pKa = 3.23VSLSKK904 pKa = 10.81DD905 pKa = 3.69VNQSTAYY912 pKa = 9.4EE913 pKa = 4.31GNRR916 pKa = 11.84VTFTITVHH924 pKa = 6.48NDD926 pKa = 3.26GPSVAHH932 pKa = 6.09NVTVTDD938 pKa = 4.39AVPDD942 pKa = 4.29GYY944 pKa = 11.38EE945 pKa = 4.01NVGDD949 pKa = 4.0ATNGASVSGSTVSFTVASLAVGADD973 pKa = 3.23ASFSFQADD981 pKa = 3.57YY982 pKa = 11.17KK983 pKa = 11.12EE984 pKa = 4.72SGEE987 pKa = 4.13HH988 pKa = 5.38TNWAEE993 pKa = 3.92VTAMDD998 pKa = 4.5EE999 pKa = 4.38ADD1001 pKa = 3.76VDD1003 pKa = 4.11SDD1005 pKa = 3.88PASDD1009 pKa = 3.87HH1010 pKa = 6.1TQDD1013 pKa = 5.21DD1014 pKa = 4.67LNDD1017 pKa = 4.18GQPDD1021 pKa = 3.91DD1022 pKa = 5.44DD1023 pKa = 4.64EE1024 pKa = 6.69SNATVSTGEE1033 pKa = 4.02KK1034 pKa = 9.13ATLGDD1039 pKa = 4.26YY1040 pKa = 10.81VWVDD1044 pKa = 3.42SNRR1047 pKa = 11.84DD1048 pKa = 3.43GIQNDD1053 pKa = 3.96GANAGIQGVTVHH1065 pKa = 6.95LYY1067 pKa = 10.81KK1068 pKa = 10.85DD1069 pKa = 3.7GQDD1072 pKa = 3.25TGRR1075 pKa = 11.84TATTDD1080 pKa = 3.29SNGKK1084 pKa = 9.18YY1085 pKa = 10.41LFDD1088 pKa = 3.77NLVPGSYY1095 pKa = 9.48HH1096 pKa = 6.8VKK1098 pKa = 9.65FDD1100 pKa = 3.55VPNGYY1105 pKa = 9.3VVSPKK1110 pKa = 10.66DD1111 pKa = 3.24QGNDD1115 pKa = 3.06EE1116 pKa = 4.76TKK1118 pKa = 11.02DD1119 pKa = 3.46SDD1121 pKa = 3.94ADD1123 pKa = 3.76PATGATVDD1131 pKa = 3.55TDD1133 pKa = 4.04LSAGEE1138 pKa = 5.22NDD1140 pKa = 4.02LTWDD1144 pKa = 3.23MGIYY1148 pKa = 9.83PVASQTASIGDD1159 pKa = 4.28YY1160 pKa = 11.13VWMDD1164 pKa = 3.82DD1165 pKa = 5.07NMNGLQDD1172 pKa = 4.89DD1173 pKa = 4.41NHH1175 pKa = 6.52PMSGVHH1181 pKa = 5.6VVLHH1185 pKa = 6.32RR1186 pKa = 11.84ADD1188 pKa = 3.49GSVVAEE1194 pKa = 4.14TDD1196 pKa = 3.76TNSSGQYY1203 pKa = 9.98YY1204 pKa = 10.36FGNLEE1209 pKa = 4.04AGDD1212 pKa = 4.4YY1213 pKa = 10.22YY1214 pKa = 11.71VSFDD1218 pKa = 3.44DD1219 pKa = 3.84QYY1221 pKa = 11.65YY1222 pKa = 8.0YY1223 pKa = 10.88TDD1225 pKa = 4.36PNVGSDD1231 pKa = 3.57DD1232 pKa = 4.49TIDD1235 pKa = 3.66SDD1237 pKa = 4.38VNRR1240 pKa = 11.84STFRR1244 pKa = 11.84TEE1246 pKa = 3.74TTHH1249 pKa = 7.13LDD1251 pKa = 2.82WGEE1254 pKa = 3.91RR1255 pKa = 11.84DD1256 pKa = 3.4MTIDD1260 pKa = 4.29AGITPTAHH1268 pKa = 6.24IGDD1271 pKa = 4.32YY1272 pKa = 10.73FWIDD1276 pKa = 3.32EE1277 pKa = 4.5NKK1279 pKa = 10.69NGIQDD1284 pKa = 3.75PGEE1287 pKa = 4.17PPVAGGGCRR1296 pKa = 11.84ALL1298 pKa = 3.86

Molecular weight: 139.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E6WYY7|E6WYY7_NITSE Cysteine desulfuration protein SufE OS=Nitratifractor salsuginis (strain DSM 16511 / JCM 12458 / E9I37-1) OX=749222 GN=Nitsa_1322 PE=3 SV=1
MM1 pKa = 7.28KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 10.04QPHH8 pKa = 4.86NTPRR12 pKa = 11.84KK13 pKa = 7.32RR14 pKa = 11.84THH16 pKa = 6.11GFRR19 pKa = 11.84ARR21 pKa = 11.84MKK23 pKa = 8.78TKK25 pKa = 10.46NGRR28 pKa = 11.84KK29 pKa = 9.3VINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.58GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2076

0

2076

630847

33

2213

303.9

34.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.281 ± 0.06

0.873 ± 0.021

5.365 ± 0.056

7.83 ± 0.083

4.298 ± 0.04

7.051 ± 0.056

2.202 ± 0.025

6.762 ± 0.051

6.422 ± 0.059

10.469 ± 0.076

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.508 ± 0.029

3.403 ± 0.049

4.319 ± 0.036

2.894 ± 0.028

6.129 ± 0.068

5.402 ± 0.04

4.68 ± 0.04

6.308 ± 0.042

1.091 ± 0.022

3.713 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski