Photobacterium halotolerans

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae; Photobacterium

Average proteome isoelectric point is 6.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3968 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0F5VC46|A0A0F5VC46_9GAMM Sensor protein OS=Photobacterium halotolerans OX=265726 GN=KY46_15130 PE=4 SV=1
MM1 pKa = 7.39KK2 pKa = 8.62QTKK5 pKa = 8.17MLSMVLLSLMLTACGGDD22 pKa = 3.27STGPVINQDD31 pKa = 3.17NLMNNSNTTSNNDD44 pKa = 3.25TSHH47 pKa = 6.88SDD49 pKa = 3.06SDD51 pKa = 3.88TTTSIDD57 pKa = 2.99IDD59 pKa = 4.41GIYY62 pKa = 10.53VDD64 pKa = 3.51VDD66 pKa = 3.61NNVVMMVDD74 pKa = 3.9EE75 pKa = 4.52DD76 pKa = 4.39TEE78 pKa = 4.99DD79 pKa = 3.38GGFVLLDD86 pKa = 3.62TDD88 pKa = 4.16DD89 pKa = 4.08SAVVMNTSHH98 pKa = 7.04TISGTRR104 pKa = 11.84MTATGISYY112 pKa = 10.37LSSSAYY118 pKa = 9.39SMNMEE123 pKa = 4.87DD124 pKa = 4.93SVTLTFYY131 pKa = 10.28RR132 pKa = 11.84QSVSFMGVINDD143 pKa = 3.48QLTSYY148 pKa = 11.29SLDD151 pKa = 3.6RR152 pKa = 11.84NSGSLSLEE160 pKa = 4.31TLDD163 pKa = 3.66GTYY166 pKa = 10.59TNSEE170 pKa = 4.06TGDD173 pKa = 3.53TFSIDD178 pKa = 3.12VAGNFTINGGCTISGTVYY196 pKa = 10.18QKK198 pKa = 10.32KK199 pKa = 9.77HH200 pKa = 5.54YY201 pKa = 9.78YY202 pKa = 9.79RR203 pKa = 11.84DD204 pKa = 3.54DD205 pKa = 3.76AASVTGCSDD214 pKa = 3.03ATFDD218 pKa = 4.23RR219 pKa = 11.84DD220 pKa = 3.55DD221 pKa = 3.53YY222 pKa = 11.83QFILFTLIVGDD233 pKa = 3.64TNYY236 pKa = 10.81LVTIADD242 pKa = 3.81SSVATLWRR250 pKa = 11.84LMPLEE255 pKa = 4.2EE256 pKa = 4.21TLL258 pKa = 4.22

Molecular weight: 28.0 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0F5VG69|A0A0F5VG69_9GAMM Fis family transcriptional regulator OS=Photobacterium halotolerans OX=265726 GN=KY46_05270 PE=4 SV=1
MM1 pKa = 7.29PCLWLFVNCSFSKK14 pKa = 10.66VDD16 pKa = 3.65EE17 pKa = 4.62KK18 pKa = 11.31LLSEE22 pKa = 4.65NKK24 pKa = 8.94PEE26 pKa = 3.61IRR28 pKa = 11.84VFHH31 pKa = 5.88CHH33 pKa = 6.25RR34 pKa = 11.84EE35 pKa = 3.81RR36 pKa = 11.84FRR38 pKa = 11.84TNFQVVGAGSAYY50 pKa = 10.11NRR52 pKa = 11.84PGQQQRR58 pKa = 11.84TKK60 pKa = 10.64RR61 pKa = 11.84GFLGTLCTHH70 pKa = 6.92KK71 pKa = 10.66AYY73 pKa = 10.58LVRR76 pKa = 11.84VQRR79 pKa = 11.84CRR81 pKa = 11.84DD82 pKa = 3.02KK83 pKa = 11.17RR84 pKa = 11.84RR85 pKa = 11.84AQVRR89 pKa = 11.84KK90 pKa = 9.2ISNPSRR96 pKa = 11.84LALVIWIAIGEE107 pKa = 4.47SMTGRR112 pKa = 11.84VYY114 pKa = 10.74FLSSNARR121 pKa = 11.84QTQGKK126 pKa = 8.85IGSEE130 pKa = 4.32FFPGSYY136 pKa = 10.06ILLKK140 pKa = 10.83LL141 pKa = 3.73

Molecular weight: 16.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3967

1

3968

1261788

31

4794

318.0

35.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.495 ± 0.043

1.061 ± 0.015

5.593 ± 0.04

5.949 ± 0.042

3.974 ± 0.029

7.073 ± 0.036

2.341 ± 0.023

5.791 ± 0.03

4.444 ± 0.036

10.735 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.733 ± 0.021

3.686 ± 0.026

4.25 ± 0.022

4.919 ± 0.032

5.038 ± 0.038

6.293 ± 0.027

5.33 ± 0.034

7.04 ± 0.035

1.312 ± 0.015

2.943 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski