candidate division MSBL1 archaeon SCGC-AAA382M17

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Euryarchaeota incertae sedis; candidate division MSBL1

Average proteome isoelectric point is 6.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 356 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A133VNX4|A0A133VNX4_9EURY Uncharacterized protein OS=candidate division MSBL1 archaeon SCGC-AAA382M17 OX=1698284 GN=AKJ55_01400 PE=4 SV=1
MM1 pKa = 7.25KK2 pKa = 10.65VKK4 pKa = 10.48GLFLILISFIFVMPVFFTSCEE25 pKa = 4.06EE26 pKa = 4.06NGEE29 pKa = 4.11EE30 pKa = 3.88TVYY33 pKa = 7.95MTEE36 pKa = 3.75KK37 pKa = 9.8TVFMLEE43 pKa = 3.82FDD45 pKa = 4.22HH46 pKa = 6.63YY47 pKa = 9.98RR48 pKa = 11.84HH49 pKa = 6.5EE50 pKa = 4.4IEE52 pKa = 4.83GDD54 pKa = 3.46QEE56 pKa = 3.92IGGDD60 pKa = 3.75KK61 pKa = 10.24DD62 pKa = 2.86WYY64 pKa = 9.87MSVKK68 pKa = 10.28FLTDD72 pKa = 3.2NLSQIPEE79 pKa = 4.21LTVNDD84 pKa = 3.76TTFTNFGFTEE94 pKa = 4.36TFLTLYY100 pKa = 10.32EE101 pKa = 4.06RR102 pKa = 11.84VPYY105 pKa = 10.79AEE107 pKa = 4.35TIEE110 pKa = 4.25YY111 pKa = 10.18SVSLGDD117 pKa = 3.54TSTSGTINMPVITEE131 pKa = 4.2ATCNGEE137 pKa = 4.0NLLVDD142 pKa = 4.22DD143 pKa = 5.69DD144 pKa = 5.37YY145 pKa = 12.01IFILEE150 pKa = 4.3ADD152 pKa = 3.78SFKK155 pKa = 11.23LKK157 pKa = 10.46WEE159 pKa = 5.5DD160 pKa = 3.28IPDD163 pKa = 3.54YY164 pKa = 11.12AIINPYY170 pKa = 10.18SYY172 pKa = 11.06QDD174 pKa = 2.98WKK176 pKa = 11.25EE177 pKa = 3.94RR178 pKa = 11.84IIDD181 pKa = 3.96TNHH184 pKa = 5.84TAIDD188 pKa = 3.65TT189 pKa = 4.04

Molecular weight: 21.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A133VQI1|A0A133VQI1_9EURY Dihydroorotate dehydrogenase (quinone) OS=candidate division MSBL1 archaeon SCGC-AAA382M17 OX=1698284 GN=AKJ55_00530 PE=3 SV=1
MM1 pKa = 7.35RR2 pKa = 11.84QDD4 pKa = 2.9EE5 pKa = 4.49KK6 pKa = 11.66ARR8 pKa = 11.84FCCGGGRR15 pKa = 11.84RR16 pKa = 11.84KK17 pKa = 9.25QVQAATPLLSAEE29 pKa = 4.51SGGGTPLHH37 pKa = 6.0PRR39 pKa = 11.84PRR41 pKa = 11.84RR42 pKa = 11.84VDD44 pKa = 3.26QQPAEE49 pKa = 4.15KK50 pKa = 10.45AEE52 pKa = 4.13SVEE55 pKa = 3.99AGKK58 pKa = 10.67KK59 pKa = 8.14NRR61 pKa = 11.84KK62 pKa = 6.43PHH64 pKa = 4.64SVRR67 pKa = 11.84RR68 pKa = 11.84NVSFRR73 pKa = 11.84EE74 pKa = 3.91SFRR77 pKa = 11.84RR78 pKa = 11.84SLRR81 pKa = 11.84GLRR84 pKa = 11.84HH85 pKa = 6.44RR86 pKa = 11.84DD87 pKa = 3.09RR88 pKa = 11.84RR89 pKa = 11.84PSPFRR94 pKa = 11.84GEE96 pKa = 5.25DD97 pKa = 3.03IPLQPGAHH105 pKa = 6.79GDD107 pKa = 3.59PGRR110 pKa = 11.84VRR112 pKa = 11.84HH113 pKa = 5.66RR114 pKa = 11.84APRR117 pKa = 11.84RR118 pKa = 11.84NEE120 pKa = 3.83GLEE123 pKa = 3.66KK124 pKa = 10.55RR125 pKa = 11.84PVRR128 pKa = 11.84GRR130 pKa = 11.84GQGPDD135 pKa = 2.99GLLRR139 pKa = 11.84QGGKK143 pKa = 7.89TSEE146 pKa = 4.13RR147 pKa = 11.84SQEE150 pKa = 4.19GAKK153 pKa = 9.95EE154 pKa = 3.86RR155 pKa = 11.84APEE158 pKa = 3.76HH159 pKa = 6.64AEE161 pKa = 3.88RR162 pKa = 11.84EE163 pKa = 4.26NAHH166 pKa = 6.76APPADD171 pKa = 3.39QDD173 pKa = 3.51QNRR176 pKa = 11.84RR177 pKa = 11.84DD178 pKa = 3.74EE179 pKa = 5.35DD180 pKa = 3.44EE181 pKa = 4.95GEE183 pKa = 4.09PTEE186 pKa = 4.48PGQAKK191 pKa = 10.08LLHH194 pKa = 6.43RR195 pKa = 11.84KK196 pKa = 9.65AGEE199 pKa = 3.79QKK201 pKa = 10.15EE202 pKa = 4.2RR203 pKa = 11.84SQCACGGFRR212 pKa = 11.84TGLL215 pKa = 3.44

Molecular weight: 24.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

356

0

356

93652

53

1109

263.1

29.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.163 ± 0.13

0.869 ± 0.046

5.559 ± 0.111

9.311 ± 0.209

4.008 ± 0.112

7.319 ± 0.149

1.739 ± 0.061

7.338 ± 0.136

7.604 ± 0.166

9.199 ± 0.147

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.23 ± 0.049

4.12 ± 0.104

4.059 ± 0.076

2.565 ± 0.067

5.541 ± 0.134

6.675 ± 0.11

4.705 ± 0.077

6.83 ± 0.125

1.061 ± 0.042

3.107 ± 0.113
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski