Hubei toti-like virus 24
Average proteome isoelectric point is 7.08
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KFA5|A0A1L3KFA5_9VIRU RdRp catalytic domain-containing protein OS=Hubei toti-like virus 24 OX=1923313 PE=4 SV=1
MM1 pKa = 7.43 TIEE4 pKa = 5.34 DD5 pKa = 3.91 KK6 pKa = 11.03 KK7 pKa = 10.78 QNCTAITQHH16 pKa = 6.83 PLFEE20 pKa = 4.64 AAQAAWAQTVPAVLPLNSGNPTHH43 pKa = 7.27 CDD45 pKa = 3.09 QVAHH49 pKa = 5.19 QAIGGAHH56 pKa = 7.17 RR57 pKa = 11.84 LHH59 pKa = 6.9 TYY61 pKa = 7.14 WQWAEE66 pKa = 3.84 PLRR69 pKa = 11.84 LVGVRR74 pKa = 11.84 PTTLALGSTILAPHH88 pKa = 6.57 LPVRR92 pKa = 11.84 VARR95 pKa = 11.84 HH96 pKa = 4.81 AQRR99 pKa = 11.84 IAGPTTDD106 pKa = 5.65 LEE108 pKa = 4.5 VTPLFQQFAALNNTPVGEE126 pKa = 4.35 FQASRR131 pKa = 11.84 LFTMXMTKK139 pKa = 9.34 DD140 pKa = 3.48 TRR142 pKa = 11.84 SMALLASVLAQGVSATKK159 pKa = 10.7 DD160 pKa = 3.07 EE161 pKa = 4.69 TPFLRR166 pKa = 11.84 AVLISEE172 pKa = 4.43 CLIKK176 pKa = 10.53 VARR179 pKa = 11.84 LPVLTQLPVRR189 pKa = 11.84 VAGDD193 pKa = 3.52 LRR195 pKa = 11.84 LTSVKK200 pKa = 10.27 RR201 pKa = 11.84 QMVKK205 pKa = 10.6 YY206 pKa = 8.95 STSAPDD212 pKa = 3.8 GNIMAVDD219 pKa = 4.16 LARR222 pKa = 11.84 FINMYY227 pKa = 10.66 ANPSDD232 pKa = 4.17 VEE234 pKa = 3.87 QHH236 pKa = 5.66 FAGFTKK242 pKa = 9.77 DD243 pKa = 2.76 TWGTTTAVVPFRR255 pKa = 11.84 TDD257 pKa = 3.07 LSGLRR262 pKa = 11.84 SSGPYY267 pKa = 9.56 VLSFVDD273 pKa = 3.2 TMYY276 pKa = 10.13 WNARR280 pKa = 11.84 IKK282 pKa = 10.75 YY283 pKa = 9.75 LLEE286 pKa = 4.49 CCPHH290 pKa = 7.2 SDD292 pKa = 4.3 DD293 pKa = 5.1 AEE295 pKa = 4.37 KK296 pKa = 11.01 VDD298 pKa = 6.89 LIAIPNAALGVVDD311 pKa = 5.1 GEE313 pKa = 4.41 ANILLVLIDD322 pKa = 3.52 QVVRR326 pKa = 11.84 TNNRR330 pKa = 11.84 FEE332 pKa = 4.89 LEE334 pKa = 4.04 VGGQKK339 pKa = 9.83 IPVSGASRR347 pKa = 11.84 VTIAPAACSVRR358 pKa = 11.84 KK359 pKa = 9.95 VVDD362 pKa = 3.53 AVFGAGGSAGDD373 pKa = 4.19 SPIGQADD380 pKa = 3.96 AYY382 pKa = 10.89 SAIKK386 pKa = 9.98 EE387 pKa = 4.16 LIGVIGTSDD396 pKa = 3.77 CYY398 pKa = 10.53 EE399 pKa = 3.96 AALILASEE407 pKa = 4.66 MCRR410 pKa = 11.84 SFPVDD415 pKa = 3.26 PLAKK419 pKa = 9.68 VGRR422 pKa = 11.84 RR423 pKa = 11.84 GDD425 pKa = 4.3 SIGLYY430 pKa = 8.37 QWEE433 pKa = 4.9 EE434 pKa = 3.8 DD435 pKa = 3.46 TMTNVLGASGPKK447 pKa = 8.19 VATWKK452 pKa = 10.51 GHH454 pKa = 6.61 FDD456 pKa = 4.17 DD457 pKa = 5.65 GLNKK461 pKa = 10.62 SKK463 pKa = 8.11 MTYY466 pKa = 8.34 LQWYY470 pKa = 9.01 AVAQSGMLPEE480 pKa = 5.36 AYY482 pKa = 10.16 FEE484 pKa = 4.28 VGSILTTDD492 pKa = 3.88 ASWPVIGSPKK502 pKa = 10.11 GGSSLIVNTADD513 pKa = 2.62 ITVRR517 pKa = 11.84 VARR520 pKa = 11.84 ALEE523 pKa = 4.06 LVYY526 pKa = 10.65 GITTRR531 pKa = 11.84 VKK533 pKa = 10.04 PDD535 pKa = 3.29 TEE537 pKa = 4.44 FTLSPWLSASAVLIASQTFAALASAGLSWASLNMLFSVHH576 pKa = 5.55 VRR578 pKa = 11.84 VPSAVEE584 pKa = 3.91 EE585 pKa = 4.34 VVSAITNGRR594 pKa = 11.84 VTTSPASKK602 pKa = 10.03 KK603 pKa = 10.32 VIYY606 pKa = 8.88 RR607 pKa = 11.84 TDD609 pKa = 2.9 IAVFSTHH616 pKa = 5.74 ATLAATGGQWAADD629 pKa = 3.8 DD630 pKa = 4.24 SGGFPLPFFLVRR642 pKa = 11.84 EE643 pKa = 4.41 LVRR646 pKa = 11.84 KK647 pKa = 9.62 FNVNLVDD654 pKa = 4.56 PGHH657 pKa = 7.12 LLQEE661 pKa = 4.29 RR662 pKa = 11.84 MKK664 pKa = 10.87 EE665 pKa = 3.88 ADD667 pKa = 3.61 EE668 pKa = 4.63 DD669 pKa = 4.17 ADD671 pKa = 4.22 IISVCAPVNKK681 pKa = 9.93 GWLFVPWYY689 pKa = 8.59 TSTVDD694 pKa = 2.97 WVRR697 pKa = 11.84 RR698 pKa = 11.84 PFTVLSTAPKK708 pKa = 8.15 GQQIRR713 pKa = 11.84 EE714 pKa = 4.08 MSVPLYY720 pKa = 8.37 WRR722 pKa = 11.84 EE723 pKa = 3.68 WADD726 pKa = 3.41 EE727 pKa = 4.17 SSAGMLRR734 pKa = 11.84 TRR736 pKa = 11.84 NVDD739 pKa = 2.61 IPARR743 pKa = 11.84 VSLYY747 pKa = 10.25 SGMMVDD753 pKa = 5.81 QFFADD758 pKa = 3.22 QFIRR762 pKa = 11.84 QEE764 pKa = 3.83 TWSEE768 pKa = 4.23 VTSATPVLCHH778 pKa = 6.63 HH779 pKa = 7.18 GDD781 pKa = 3.38 VVADD785 pKa = 3.56 VTVPDD790 pKa = 4.63 PEE792 pKa = 4.74 SPLLRR797 pKa = 11.84 AVWSDD802 pKa = 2.72 SHH804 pKa = 7.17 GFVPPILALEE814 pKa = 4.44 APVDD818 pKa = 3.89 VDD820 pKa = 4.51 GVLPPSGPARR830 pKa = 11.84 TNGQRR835 pKa = 11.84 LSRR838 pKa = 11.84 VLQLPFGASDD848 pKa = 4.01 LGRR851 pKa = 11.84 ARR853 pKa = 11.84 CSPPKK858 pKa = 10.18 AEE860 pKa = 4.33 SSGTPGLGVNVVPTPRR876 pKa = 11.84 RR877 pKa = 11.84 EE878 pKa = 4.04 LRR880 pKa = 11.84 NRR882 pKa = 11.84 DD883 pKa = 3.52 PLGLLGLNRR892 pKa = 11.84 VGGQQAQQPPLNPPPPPAGADD913 pKa = 3.22 PALANRR919 pKa = 11.84 GAGQPAGAGNDD930 pKa = 3.86 DD931 pKa = 3.44 QHH933 pKa = 8.56 DD934 pKa = 4.02 PDD936 pKa = 4.13 SRR938 pKa = 3.95
Molecular weight: 100.86 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.761
IPC2_protein 5.664
IPC_protein 5.715
Toseland 6.084
ProMoST 6.059
Dawson 5.97
Bjellqvist 5.982
Wikipedia 5.97
Rodwell 5.944
Grimsley 6.224
Solomon 5.97
Lehninger 5.957
Nozaki 6.224
DTASelect 6.427
Thurlkill 6.415
EMBOSS 6.376
Sillero 6.326
Patrickios 4.355
IPC_peptide 5.982
IPC2_peptide 6.326
IPC2.peptide.svr19 6.272
Protein with the highest isoelectric point:
>tr|A0A1L3KFA5|A0A1L3KFA5_9VIRU RdRp catalytic domain-containing protein OS=Hubei toti-like virus 24 OX=1923313 PE=4 SV=1
MM1 pKa = 7.39 MISTIRR7 pKa = 11.84 TLGRR11 pKa = 11.84 WAGPRR16 pKa = 11.84 PTAKK20 pKa = 10.17 LGHH23 pKa = 6.36 FGGGRR28 pKa = 11.84 VDD30 pKa = 4.97 GKK32 pKa = 9.74 LTLDD36 pKa = 3.63 WAWVSGFEE44 pKa = 4.25 FLPAGTALRR53 pKa = 11.84 TLLKK57 pKa = 10.65 GCDD60 pKa = 3.26 VARR63 pKa = 11.84 NFARR67 pKa = 11.84 LEE69 pKa = 3.71 GLRR72 pKa = 11.84 RR73 pKa = 11.84 SRR75 pKa = 11.84 YY76 pKa = 7.51 TSVKK80 pKa = 8.87 HH81 pKa = 4.68 TQQLVTWAGSFAGPLAFLLDD101 pKa = 3.74 MSEE104 pKa = 4.22 AAAAADD110 pKa = 3.33 PVVPFFYY117 pKa = 10.67 HH118 pKa = 7.17 LFVTTAKK125 pKa = 10.5 ARR127 pKa = 11.84 VEE129 pKa = 4.07 MAVEE133 pKa = 4.15 VLSPDD138 pKa = 3.63 STVSWPGVPKK148 pKa = 10.92 LPDD151 pKa = 3.85 HH152 pKa = 6.87 PDD154 pKa = 3.18 EE155 pKa = 4.39 YY156 pKa = 11.04 RR157 pKa = 11.84 DD158 pKa = 4.05 DD159 pKa = 3.71 IVRR162 pKa = 11.84 MVIKK166 pKa = 10.63 DD167 pKa = 3.43 MEE169 pKa = 4.53 EE170 pKa = 4.06 EE171 pKa = 4.36 VSSHH175 pKa = 6.28 GVDD178 pKa = 3.03 ARR180 pKa = 11.84 LAISGLYY187 pKa = 9.24 TSGGPRR193 pKa = 11.84 GWDD196 pKa = 3.2 RR197 pKa = 11.84 EE198 pKa = 4.19 HH199 pKa = 6.24 MVDD202 pKa = 5.1 YY203 pKa = 10.41 VKK205 pKa = 10.84 SWIQGEE211 pKa = 4.31 APLFSARR218 pKa = 11.84 EE219 pKa = 3.66 EE220 pKa = 4.01 WVRR223 pKa = 11.84 AKK225 pKa = 10.76 LRR227 pKa = 11.84 FWFSSWVKK235 pKa = 10.21 LAPPDD240 pKa = 3.67 GAGGFDD246 pKa = 3.47 FEE248 pKa = 4.51 QYY250 pKa = 10.45 KK251 pKa = 10.36 RR252 pKa = 11.84 DD253 pKa = 3.65 VIRR256 pKa = 11.84 WGTSGGASPPPEE268 pKa = 3.52 VRR270 pKa = 11.84 EE271 pKa = 3.93 RR272 pKa = 11.84 LEE274 pKa = 4.24 SLFPDD279 pKa = 4.02 LPKK282 pKa = 10.64 SAFRR286 pKa = 11.84 TKK288 pKa = 7.8 TTAGISSLLFGEE300 pKa = 5.17 LEE302 pKa = 5.31 DD303 pKa = 4.61 FFKK306 pKa = 11.1 PNNVCHH312 pKa = 6.59 AALKK316 pKa = 9.72 EE317 pKa = 3.92 XAKK320 pKa = 9.98 TRR322 pKa = 11.84 IIVSTPMHH330 pKa = 6.84 SYY332 pKa = 10.88 VAMCFVFDD340 pKa = 5.2 LLGPPHH346 pKa = 6.5 FLKK349 pKa = 10.02 STIASSRR356 pKa = 11.84 GLEE359 pKa = 3.66 EE360 pKa = 3.68 FARR363 pKa = 11.84 YY364 pKa = 7.78 RR365 pKa = 11.84 AKK367 pKa = 10.78 YY368 pKa = 8.55 YY369 pKa = 10.96 AAIDD373 pKa = 3.6 ASKK376 pKa = 10.47 FDD378 pKa = 4.21 HH379 pKa = 6.79 NVPRR383 pKa = 11.84 WLLEE387 pKa = 4.21 MIWDD391 pKa = 4.01 EE392 pKa = 4.34 LALALEE398 pKa = 4.51 SRR400 pKa = 11.84 CDD402 pKa = 3.61 EE403 pKa = 4.32 RR404 pKa = 11.84 FTTASHH410 pKa = 7.15 LCRR413 pKa = 11.84 SLSSEE418 pKa = 3.69 LRR420 pKa = 11.84 NLVVEE425 pKa = 4.12 ILGTHH430 pKa = 6.02 LRR432 pKa = 11.84 YY433 pKa = 9.89 EE434 pKa = 4.33 KK435 pKa = 10.91 GLLSGWRR442 pKa = 11.84 ATSFIGSLVSALCCEE457 pKa = 4.98 SYY459 pKa = 11.01 VEE461 pKa = 4.24 HH462 pKa = 6.81 HH463 pKa = 6.71 ASASRR468 pKa = 11.84 SEE470 pKa = 3.82 YY471 pKa = 10.43 RR472 pKa = 11.84 HH473 pKa = 5.91 WIDD476 pKa = 4.44 YY477 pKa = 9.67 LVQGDD482 pKa = 4.72 DD483 pKa = 3.86 IILFSSAYY491 pKa = 9.83 DD492 pKa = 3.58 LSDD495 pKa = 3.04 SVDD498 pKa = 3.34 YY499 pKa = 10.39 MRR501 pKa = 11.84 GLGVLTHH508 pKa = 6.85 PSKK511 pKa = 11.07 CLIGADD517 pKa = 3.84 GDD519 pKa = 3.91 FLRR522 pKa = 11.84 AIYY525 pKa = 9.68 TKK527 pKa = 10.22 DD528 pKa = 3.12 GRR530 pKa = 11.84 KK531 pKa = 7.33 TYY533 pKa = 10.39 PMRR536 pKa = 11.84 ALRR539 pKa = 11.84 SIFYY543 pKa = 10.22 ANPWVEE549 pKa = 3.72 QRR551 pKa = 11.84 AFKK554 pKa = 10.8 GVVEE558 pKa = 5.11 ISQNWLVVEE567 pKa = 4.5 SRR569 pKa = 11.84 FSLLCTTSDD578 pKa = 3.0 VEE580 pKa = 4.14 RR581 pKa = 11.84 AVRR584 pKa = 11.84 SAACADD590 pKa = 3.27 IARR593 pKa = 11.84 WSGGSLRR600 pKa = 11.84 ASQVRR605 pKa = 11.84 ALYY608 pKa = 7.55 NTPLSVGGLGPVEE621 pKa = 4.55 SVFGTTVTTLTVDD634 pKa = 3.28 YY635 pKa = 10.76 DD636 pKa = 3.97 VYY638 pKa = 11.37 AEE640 pKa = 4.2 KK641 pKa = 10.07 TRR643 pKa = 11.84 SVLPSLWQFVGVTVPLKK660 pKa = 8.78 PSKK663 pKa = 10.51 RR664 pKa = 11.84 FTLQSVDD671 pKa = 2.99 IFSLRR676 pKa = 11.84 NLAKK680 pKa = 10.3 KK681 pKa = 10.55 AFFTSVPHH689 pKa = 7.14 DD690 pKa = 4.03 LDD692 pKa = 3.7 KK693 pKa = 11.55 KK694 pKa = 10.48 GVNLTSLFLSVIYY707 pKa = 8.76 DD708 pKa = 3.69 TEE710 pKa = 4.2 ARR712 pKa = 11.84 RR713 pKa = 11.84 HH714 pKa = 4.76 TVDD717 pKa = 2.97 AGSFEE722 pKa = 4.52 VVALRR727 pKa = 11.84 RR728 pKa = 11.84 ALSIVGYY735 pKa = 8.39 TDD737 pKa = 3.0 YY738 pKa = 11.24 RR739 pKa = 11.84 PFVGGIYY746 pKa = 10.13 RR747 pKa = 11.84 CTTRR751 pKa = 11.84 ASEE754 pKa = 4.22 VLSLLFSGKK763 pKa = 10.1 DD764 pKa = 3.24 PSVPPSVYY772 pKa = 10.88 QMTSPVSRR780 pKa = 11.84 AVKK783 pKa = 9.59 WLASLLGRR791 pKa = 11.84 WLRR794 pKa = 11.84 YY795 pKa = 8.92 KK796 pKa = 10.71 ARR798 pKa = 11.84 TVKK801 pKa = 10.32 GVRR804 pKa = 11.84 AWSAGYY810 pKa = 8.11 TLLCSAITVSTVKK823 pKa = 9.94 GTMM826 pKa = 3.24
Molecular weight: 92.02 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.395
IPC2_protein 8.361
IPC_protein 8.244
Toseland 8.565
ProMoST 8.741
Dawson 9.077
Bjellqvist 9.107
Wikipedia 9.209
Rodwell 9.165
Grimsley 8.946
Solomon 9.136
Lehninger 9.092
Nozaki 9.18
DTASelect 8.946
Thurlkill 9.019
EMBOSS 9.209
Sillero 9.224
Patrickios 4.558
IPC_peptide 9.121
IPC2_peptide 7.922
IPC2.peptide.svr19 7.725
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1764
826
938
882.0
96.44
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.921 ± 0.818
1.304 ± 0.101
5.556 ± 0.155
4.422 ± 0.368
4.082 ± 0.599
7.2 ± 0.121
1.984 ± 0.132
3.685 ± 0.2
3.571 ± 0.535
9.751 ± 0.367
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.814 ± 0.081
2.211 ± 0.679
6.122 ± 0.952
2.721 ± 0.944
6.689 ± 0.719
8.333 ± 0.836
6.463 ± 0.196
9.127 ± 0.361
2.324 ± 0.23
2.608 ± 0.613
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here