Hubei toti-like virus 24

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1L3KFA5|A0A1L3KFA5_9VIRU RdRp catalytic domain-containing protein OS=Hubei toti-like virus 24 OX=1923313 PE=4 SV=1
MM1 pKa = 7.43TIEE4 pKa = 5.34DD5 pKa = 3.91KK6 pKa = 11.03KK7 pKa = 10.78QNCTAITQHH16 pKa = 6.83PLFEE20 pKa = 4.64AAQAAWAQTVPAVLPLNSGNPTHH43 pKa = 7.27CDD45 pKa = 3.09QVAHH49 pKa = 5.19QAIGGAHH56 pKa = 7.17RR57 pKa = 11.84LHH59 pKa = 6.9TYY61 pKa = 7.14WQWAEE66 pKa = 3.84PLRR69 pKa = 11.84LVGVRR74 pKa = 11.84PTTLALGSTILAPHH88 pKa = 6.57LPVRR92 pKa = 11.84VARR95 pKa = 11.84HH96 pKa = 4.81AQRR99 pKa = 11.84IAGPTTDD106 pKa = 5.65LEE108 pKa = 4.5VTPLFQQFAALNNTPVGEE126 pKa = 4.35FQASRR131 pKa = 11.84LFTMXMTKK139 pKa = 9.34DD140 pKa = 3.48TRR142 pKa = 11.84SMALLASVLAQGVSATKK159 pKa = 10.7DD160 pKa = 3.07EE161 pKa = 4.69TPFLRR166 pKa = 11.84AVLISEE172 pKa = 4.43CLIKK176 pKa = 10.53VARR179 pKa = 11.84LPVLTQLPVRR189 pKa = 11.84VAGDD193 pKa = 3.52LRR195 pKa = 11.84LTSVKK200 pKa = 10.27RR201 pKa = 11.84QMVKK205 pKa = 10.6YY206 pKa = 8.95STSAPDD212 pKa = 3.8GNIMAVDD219 pKa = 4.16LARR222 pKa = 11.84FINMYY227 pKa = 10.66ANPSDD232 pKa = 4.17VEE234 pKa = 3.87QHH236 pKa = 5.66FAGFTKK242 pKa = 9.77DD243 pKa = 2.76TWGTTTAVVPFRR255 pKa = 11.84TDD257 pKa = 3.07LSGLRR262 pKa = 11.84SSGPYY267 pKa = 9.56VLSFVDD273 pKa = 3.2TMYY276 pKa = 10.13WNARR280 pKa = 11.84IKK282 pKa = 10.75YY283 pKa = 9.75LLEE286 pKa = 4.49CCPHH290 pKa = 7.2SDD292 pKa = 4.3DD293 pKa = 5.1AEE295 pKa = 4.37KK296 pKa = 11.01VDD298 pKa = 6.89LIAIPNAALGVVDD311 pKa = 5.1GEE313 pKa = 4.41ANILLVLIDD322 pKa = 3.52QVVRR326 pKa = 11.84TNNRR330 pKa = 11.84FEE332 pKa = 4.89LEE334 pKa = 4.04VGGQKK339 pKa = 9.83IPVSGASRR347 pKa = 11.84VTIAPAACSVRR358 pKa = 11.84KK359 pKa = 9.95VVDD362 pKa = 3.53AVFGAGGSAGDD373 pKa = 4.19SPIGQADD380 pKa = 3.96AYY382 pKa = 10.89SAIKK386 pKa = 9.98EE387 pKa = 4.16LIGVIGTSDD396 pKa = 3.77CYY398 pKa = 10.53EE399 pKa = 3.96AALILASEE407 pKa = 4.66MCRR410 pKa = 11.84SFPVDD415 pKa = 3.26PLAKK419 pKa = 9.68VGRR422 pKa = 11.84RR423 pKa = 11.84GDD425 pKa = 4.3SIGLYY430 pKa = 8.37QWEE433 pKa = 4.9EE434 pKa = 3.8DD435 pKa = 3.46TMTNVLGASGPKK447 pKa = 8.19VATWKK452 pKa = 10.51GHH454 pKa = 6.61FDD456 pKa = 4.17DD457 pKa = 5.65GLNKK461 pKa = 10.62SKK463 pKa = 8.11MTYY466 pKa = 8.34LQWYY470 pKa = 9.01AVAQSGMLPEE480 pKa = 5.36AYY482 pKa = 10.16FEE484 pKa = 4.28VGSILTTDD492 pKa = 3.88ASWPVIGSPKK502 pKa = 10.11GGSSLIVNTADD513 pKa = 2.62ITVRR517 pKa = 11.84VARR520 pKa = 11.84ALEE523 pKa = 4.06LVYY526 pKa = 10.65GITTRR531 pKa = 11.84VKK533 pKa = 10.04PDD535 pKa = 3.29TEE537 pKa = 4.44FTLSPWLSASAVLIASQTFAALASAGLSWASLNMLFSVHH576 pKa = 5.55VRR578 pKa = 11.84VPSAVEE584 pKa = 3.91EE585 pKa = 4.34VVSAITNGRR594 pKa = 11.84VTTSPASKK602 pKa = 10.03KK603 pKa = 10.32VIYY606 pKa = 8.88RR607 pKa = 11.84TDD609 pKa = 2.9IAVFSTHH616 pKa = 5.74ATLAATGGQWAADD629 pKa = 3.8DD630 pKa = 4.24SGGFPLPFFLVRR642 pKa = 11.84EE643 pKa = 4.41LVRR646 pKa = 11.84KK647 pKa = 9.62FNVNLVDD654 pKa = 4.56PGHH657 pKa = 7.12LLQEE661 pKa = 4.29RR662 pKa = 11.84MKK664 pKa = 10.87EE665 pKa = 3.88ADD667 pKa = 3.61EE668 pKa = 4.63DD669 pKa = 4.17ADD671 pKa = 4.22IISVCAPVNKK681 pKa = 9.93GWLFVPWYY689 pKa = 8.59TSTVDD694 pKa = 2.97WVRR697 pKa = 11.84RR698 pKa = 11.84PFTVLSTAPKK708 pKa = 8.15GQQIRR713 pKa = 11.84EE714 pKa = 4.08MSVPLYY720 pKa = 8.37WRR722 pKa = 11.84EE723 pKa = 3.68WADD726 pKa = 3.41EE727 pKa = 4.17SSAGMLRR734 pKa = 11.84TRR736 pKa = 11.84NVDD739 pKa = 2.61IPARR743 pKa = 11.84VSLYY747 pKa = 10.25SGMMVDD753 pKa = 5.81QFFADD758 pKa = 3.22QFIRR762 pKa = 11.84QEE764 pKa = 3.83TWSEE768 pKa = 4.23VTSATPVLCHH778 pKa = 6.63HH779 pKa = 7.18GDD781 pKa = 3.38VVADD785 pKa = 3.56VTVPDD790 pKa = 4.63PEE792 pKa = 4.74SPLLRR797 pKa = 11.84AVWSDD802 pKa = 2.72SHH804 pKa = 7.17GFVPPILALEE814 pKa = 4.44APVDD818 pKa = 3.89VDD820 pKa = 4.51GVLPPSGPARR830 pKa = 11.84TNGQRR835 pKa = 11.84LSRR838 pKa = 11.84VLQLPFGASDD848 pKa = 4.01LGRR851 pKa = 11.84ARR853 pKa = 11.84CSPPKK858 pKa = 10.18AEE860 pKa = 4.33SSGTPGLGVNVVPTPRR876 pKa = 11.84RR877 pKa = 11.84EE878 pKa = 4.04LRR880 pKa = 11.84NRR882 pKa = 11.84DD883 pKa = 3.52PLGLLGLNRR892 pKa = 11.84VGGQQAQQPPLNPPPPPAGADD913 pKa = 3.22PALANRR919 pKa = 11.84GAGQPAGAGNDD930 pKa = 3.86DD931 pKa = 3.44QHH933 pKa = 8.56DD934 pKa = 4.02PDD936 pKa = 4.13SRR938 pKa = 3.95

Molecular weight:
100.86 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1L3KFA5|A0A1L3KFA5_9VIRU RdRp catalytic domain-containing protein OS=Hubei toti-like virus 24 OX=1923313 PE=4 SV=1
MM1 pKa = 7.39MISTIRR7 pKa = 11.84TLGRR11 pKa = 11.84WAGPRR16 pKa = 11.84PTAKK20 pKa = 10.17LGHH23 pKa = 6.36FGGGRR28 pKa = 11.84VDD30 pKa = 4.97GKK32 pKa = 9.74LTLDD36 pKa = 3.63WAWVSGFEE44 pKa = 4.25FLPAGTALRR53 pKa = 11.84TLLKK57 pKa = 10.65GCDD60 pKa = 3.26VARR63 pKa = 11.84NFARR67 pKa = 11.84LEE69 pKa = 3.71GLRR72 pKa = 11.84RR73 pKa = 11.84SRR75 pKa = 11.84YY76 pKa = 7.51TSVKK80 pKa = 8.87HH81 pKa = 4.68TQQLVTWAGSFAGPLAFLLDD101 pKa = 3.74MSEE104 pKa = 4.22AAAAADD110 pKa = 3.33PVVPFFYY117 pKa = 10.67HH118 pKa = 7.17LFVTTAKK125 pKa = 10.5ARR127 pKa = 11.84VEE129 pKa = 4.07MAVEE133 pKa = 4.15VLSPDD138 pKa = 3.63STVSWPGVPKK148 pKa = 10.92LPDD151 pKa = 3.85HH152 pKa = 6.87PDD154 pKa = 3.18EE155 pKa = 4.39YY156 pKa = 11.04RR157 pKa = 11.84DD158 pKa = 4.05DD159 pKa = 3.71IVRR162 pKa = 11.84MVIKK166 pKa = 10.63DD167 pKa = 3.43MEE169 pKa = 4.53EE170 pKa = 4.06EE171 pKa = 4.36VSSHH175 pKa = 6.28GVDD178 pKa = 3.03ARR180 pKa = 11.84LAISGLYY187 pKa = 9.24TSGGPRR193 pKa = 11.84GWDD196 pKa = 3.2RR197 pKa = 11.84EE198 pKa = 4.19HH199 pKa = 6.24MVDD202 pKa = 5.1YY203 pKa = 10.41VKK205 pKa = 10.84SWIQGEE211 pKa = 4.31APLFSARR218 pKa = 11.84EE219 pKa = 3.66EE220 pKa = 4.01WVRR223 pKa = 11.84AKK225 pKa = 10.76LRR227 pKa = 11.84FWFSSWVKK235 pKa = 10.21LAPPDD240 pKa = 3.67GAGGFDD246 pKa = 3.47FEE248 pKa = 4.51QYY250 pKa = 10.45KK251 pKa = 10.36RR252 pKa = 11.84DD253 pKa = 3.65VIRR256 pKa = 11.84WGTSGGASPPPEE268 pKa = 3.52VRR270 pKa = 11.84EE271 pKa = 3.93RR272 pKa = 11.84LEE274 pKa = 4.24SLFPDD279 pKa = 4.02LPKK282 pKa = 10.64SAFRR286 pKa = 11.84TKK288 pKa = 7.8TTAGISSLLFGEE300 pKa = 5.17LEE302 pKa = 5.31DD303 pKa = 4.61FFKK306 pKa = 11.1PNNVCHH312 pKa = 6.59AALKK316 pKa = 9.72EE317 pKa = 3.92XAKK320 pKa = 9.98TRR322 pKa = 11.84IIVSTPMHH330 pKa = 6.84SYY332 pKa = 10.88VAMCFVFDD340 pKa = 5.2LLGPPHH346 pKa = 6.5FLKK349 pKa = 10.02STIASSRR356 pKa = 11.84GLEE359 pKa = 3.66EE360 pKa = 3.68FARR363 pKa = 11.84YY364 pKa = 7.78RR365 pKa = 11.84AKK367 pKa = 10.78YY368 pKa = 8.55YY369 pKa = 10.96AAIDD373 pKa = 3.6ASKK376 pKa = 10.47FDD378 pKa = 4.21HH379 pKa = 6.79NVPRR383 pKa = 11.84WLLEE387 pKa = 4.21MIWDD391 pKa = 4.01EE392 pKa = 4.34LALALEE398 pKa = 4.51SRR400 pKa = 11.84CDD402 pKa = 3.61EE403 pKa = 4.32RR404 pKa = 11.84FTTASHH410 pKa = 7.15LCRR413 pKa = 11.84SLSSEE418 pKa = 3.69LRR420 pKa = 11.84NLVVEE425 pKa = 4.12ILGTHH430 pKa = 6.02LRR432 pKa = 11.84YY433 pKa = 9.89EE434 pKa = 4.33KK435 pKa = 10.91GLLSGWRR442 pKa = 11.84ATSFIGSLVSALCCEE457 pKa = 4.98SYY459 pKa = 11.01VEE461 pKa = 4.24HH462 pKa = 6.81HH463 pKa = 6.71ASASRR468 pKa = 11.84SEE470 pKa = 3.82YY471 pKa = 10.43RR472 pKa = 11.84HH473 pKa = 5.91WIDD476 pKa = 4.44YY477 pKa = 9.67LVQGDD482 pKa = 4.72DD483 pKa = 3.86IILFSSAYY491 pKa = 9.83DD492 pKa = 3.58LSDD495 pKa = 3.04SVDD498 pKa = 3.34YY499 pKa = 10.39MRR501 pKa = 11.84GLGVLTHH508 pKa = 6.85PSKK511 pKa = 11.07CLIGADD517 pKa = 3.84GDD519 pKa = 3.91FLRR522 pKa = 11.84AIYY525 pKa = 9.68TKK527 pKa = 10.22DD528 pKa = 3.12GRR530 pKa = 11.84KK531 pKa = 7.33TYY533 pKa = 10.39PMRR536 pKa = 11.84ALRR539 pKa = 11.84SIFYY543 pKa = 10.22ANPWVEE549 pKa = 3.72QRR551 pKa = 11.84AFKK554 pKa = 10.8GVVEE558 pKa = 5.11ISQNWLVVEE567 pKa = 4.5SRR569 pKa = 11.84FSLLCTTSDD578 pKa = 3.0VEE580 pKa = 4.14RR581 pKa = 11.84AVRR584 pKa = 11.84SAACADD590 pKa = 3.27IARR593 pKa = 11.84WSGGSLRR600 pKa = 11.84ASQVRR605 pKa = 11.84ALYY608 pKa = 7.55NTPLSVGGLGPVEE621 pKa = 4.55SVFGTTVTTLTVDD634 pKa = 3.28YY635 pKa = 10.76DD636 pKa = 3.97VYY638 pKa = 11.37AEE640 pKa = 4.2KK641 pKa = 10.07TRR643 pKa = 11.84SVLPSLWQFVGVTVPLKK660 pKa = 8.78PSKK663 pKa = 10.51RR664 pKa = 11.84FTLQSVDD671 pKa = 2.99IFSLRR676 pKa = 11.84NLAKK680 pKa = 10.3KK681 pKa = 10.55AFFTSVPHH689 pKa = 7.14DD690 pKa = 4.03LDD692 pKa = 3.7KK693 pKa = 11.55KK694 pKa = 10.48GVNLTSLFLSVIYY707 pKa = 8.76DD708 pKa = 3.69TEE710 pKa = 4.2ARR712 pKa = 11.84RR713 pKa = 11.84HH714 pKa = 4.76TVDD717 pKa = 2.97AGSFEE722 pKa = 4.52VVALRR727 pKa = 11.84RR728 pKa = 11.84ALSIVGYY735 pKa = 8.39TDD737 pKa = 3.0YY738 pKa = 11.24RR739 pKa = 11.84PFVGGIYY746 pKa = 10.13RR747 pKa = 11.84CTTRR751 pKa = 11.84ASEE754 pKa = 4.22VLSLLFSGKK763 pKa = 10.1DD764 pKa = 3.24PSVPPSVYY772 pKa = 10.88QMTSPVSRR780 pKa = 11.84AVKK783 pKa = 9.59WLASLLGRR791 pKa = 11.84WLRR794 pKa = 11.84YY795 pKa = 8.92KK796 pKa = 10.71ARR798 pKa = 11.84TVKK801 pKa = 10.32GVRR804 pKa = 11.84AWSAGYY810 pKa = 8.11TLLCSAITVSTVKK823 pKa = 9.94GTMM826 pKa = 3.24

Molecular weight:
92.02 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1764

826

938

882.0

96.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.921 ± 0.818

1.304 ± 0.101

5.556 ± 0.155

4.422 ± 0.368

4.082 ± 0.599

7.2 ± 0.121

1.984 ± 0.132

3.685 ± 0.2

3.571 ± 0.535

9.751 ± 0.367

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.814 ± 0.081

2.211 ± 0.679

6.122 ± 0.952

2.721 ± 0.944

6.689 ± 0.719

8.333 ± 0.836

6.463 ± 0.196

9.127 ± 0.361

2.324 ± 0.23

2.608 ± 0.613

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski