Coconut foliar decay alphasatellite 7
Average proteome isoelectric point is 5.57
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2R4N9C1|A0A2R4N9C1_9VIRU Uncharacterized protein OS=Coconut foliar decay alphasatellite 7 OX=2161880 PE=4 SV=1
MM1 pKa = 8.51 DD2 pKa = 4.1 YY3 pKa = 11.08 LKK5 pKa = 10.3 PNNSISSVSLSSCTFYY21 pKa = 11.5 KK22 pKa = 10.8 LLVLQYY28 pKa = 10.74 EE29 pKa = 4.67 VLQHH33 pKa = 6.84 LLRR36 pKa = 11.84 TFHH39 pKa = 7.1 KK40 pKa = 10.79 AFVSNQDD47 pKa = 2.85 ASEE50 pKa = 3.93 LHH52 pKa = 5.66 VRR54 pKa = 11.84 KK55 pKa = 9.19 EE56 pKa = 4.02 HH57 pKa = 6.24 FLSNNKK63 pKa = 10.11 SYY65 pKa = 10.44 FADD68 pKa = 3.82 QIPSPVLNAQYY79 pKa = 10.47 YY80 pKa = 10.58 SLMSSSNLQAYY91 pKa = 9.81 GPFLQDD97 pKa = 3.19 EE98 pKa = 5.02 EE99 pKa = 4.41 ILAGAFVQSS108 pKa = 3.67
Molecular weight: 12.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.479
IPC2_protein 5.728
IPC_protein 5.753
Toseland 6.262
ProMoST 6.084
Dawson 6.046
Bjellqvist 6.02
Wikipedia 6.071
Rodwell 6.033
Grimsley 6.453
Solomon 6.046
Lehninger 6.046
Nozaki 6.313
DTASelect 6.517
Thurlkill 6.532
EMBOSS 6.504
Sillero 6.427
Patrickios 2.142
IPC_peptide 6.059
IPC2_peptide 6.415
IPC2.peptide.svr19 6.37
Protein with the highest isoelectric point:
>tr|A0A2R4N9C1|A0A2R4N9C1_9VIRU Uncharacterized protein OS=Coconut foliar decay alphasatellite 7 OX=2161880 PE=4 SV=1
MM1 pKa = 7.64 ASQVRR6 pKa = 11.84 RR7 pKa = 11.84 WVLTFNYY14 pKa = 9.27 SDD16 pKa = 3.87 EE17 pKa = 4.44 SAASDD22 pKa = 3.34 LVRR25 pKa = 11.84 RR26 pKa = 11.84 IEE28 pKa = 4.11 SLKK31 pKa = 10.62 LIYY34 pKa = 10.16 GIIGDD39 pKa = 4.99 EE40 pKa = 4.06 IAPTTGQRR48 pKa = 11.84 HH49 pKa = 4.74 LQGFLHH55 pKa = 6.47 LAGRR59 pKa = 11.84 GRR61 pKa = 11.84 TLEE64 pKa = 4.04 GLKK67 pKa = 8.93 KK68 pKa = 8.74 TLEE71 pKa = 4.22 NNTVHH76 pKa = 7.11 LEE78 pKa = 3.9 PAKK81 pKa = 10.96 GSDD84 pKa = 3.59 QQNKK88 pKa = 9.47 IYY90 pKa = 10.28 CSKK93 pKa = 10.69 EE94 pKa = 3.58 NVLFEE99 pKa = 4.83 HH100 pKa = 7.03 GVPTRR105 pKa = 11.84 PGSKK109 pKa = 8.68 RR110 pKa = 11.84 KK111 pKa = 9.79 LYY113 pKa = 10.34 EE114 pKa = 4.04 RR115 pKa = 11.84 YY116 pKa = 10.07 EE117 pKa = 4.06 EE118 pKa = 4.29 DD119 pKa = 3.68 AEE121 pKa = 4.09 EE122 pKa = 4.54 LRR124 pKa = 11.84 IEE126 pKa = 4.18 EE127 pKa = 4.13 PGAYY131 pKa = 9.04 RR132 pKa = 11.84 RR133 pKa = 11.84 CKK135 pKa = 10.05 MIEE138 pKa = 3.95 KK139 pKa = 9.13 QKK141 pKa = 10.57 KK142 pKa = 6.14 WNYY145 pKa = 8.45 WVLNNPFPYY154 pKa = 10.28 DD155 pKa = 3.75 LYY157 pKa = 10.68 QWQEE161 pKa = 3.65 EE162 pKa = 4.27 LMEE165 pKa = 4.3 VLQEE169 pKa = 4.09 PADD172 pKa = 3.59 NRR174 pKa = 11.84 TILWICGRR182 pKa = 11.84 DD183 pKa = 3.5 GGDD186 pKa = 2.94 GKK188 pKa = 9.12 TEE190 pKa = 3.9 FGKK193 pKa = 11.05 YY194 pKa = 9.9 LGLNEE199 pKa = 4.03 KK200 pKa = 9.22 WFYY203 pKa = 11.15 SCGGKK208 pKa = 7.83 TRR210 pKa = 11.84 DD211 pKa = 3.3 VLYY214 pKa = 10.55 QYY216 pKa = 10.6 IEE218 pKa = 3.91 EE219 pKa = 4.4 PEE221 pKa = 4.12 RR222 pKa = 11.84 NFILDD227 pKa = 3.44 VPRR230 pKa = 11.84 CNDD233 pKa = 2.51 QYY235 pKa = 11.41 MNYY238 pKa = 11.05 ALIEE242 pKa = 4.52 CIKK245 pKa = 10.66 NRR247 pKa = 11.84 AFSSDD252 pKa = 3.0 KK253 pKa = 11.21 YY254 pKa = 11.25 EE255 pKa = 4.06 PLQYY259 pKa = 11.02 LGFDD263 pKa = 3.33 KK264 pKa = 11.23 VHH266 pKa = 6.87 VIVLCNFLPDD276 pKa = 3.64 YY277 pKa = 11.15 LKK279 pKa = 10.63 ISEE282 pKa = 4.88 DD283 pKa = 4.93 RR284 pKa = 11.84 IKK286 pKa = 10.8 LWNII290 pKa = 3.27
Molecular weight: 34.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.669
IPC2_protein 5.83
IPC_protein 5.83
Toseland 6.033
ProMoST 6.122
Dawson 6.033
Bjellqvist 6.059
Wikipedia 6.008
Rodwell 6.008
Grimsley 6.097
Solomon 6.033
Lehninger 6.02
Nozaki 6.262
DTASelect 6.427
Thurlkill 6.415
EMBOSS 6.376
Sillero 6.364
Patrickios 3.986
IPC_peptide 6.046
IPC2_peptide 6.313
IPC2.peptide.svr19 6.408
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
398
108
290
199.0
23.24
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.523 ± 0.968
2.01 ± 0.536
5.025 ± 0.653
8.291 ± 1.809
4.271 ± 1.092
5.528 ± 1.816
2.261 ± 0.713
5.276 ± 1.234
6.533 ± 0.94
11.307 ± 1.276
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.508 ± 0.17
5.779 ± 0.347
4.02 ± 0.156
5.025 ± 1.177
5.528 ± 1.816
7.035 ± 3.843
3.015 ± 0.575
5.025 ± 0.719
1.759 ± 0.869
6.281 ± 0.099
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here