Akodon montensis polyomavirus
Average proteome isoelectric point is 7.11
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A343W7C6|A0A343W7C6_9POLY VP1 protein OS=Akodon montensis polyomavirus OX=2163422 PE=3 SV=1
MM1 pKa = 7.73 ACQTQGRR8 pKa = 11.84 KK9 pKa = 9.32 RR10 pKa = 11.84 LGPGKK15 pKa = 9.96 APKK18 pKa = 10.01 VAEE21 pKa = 3.87 QRR23 pKa = 11.84 LPRR26 pKa = 11.84 ILRR29 pKa = 11.84 KK30 pKa = 10.37 GGVEE34 pKa = 3.96 VLGTVPLSEE43 pKa = 3.94 HH44 pKa = 4.0 TQYY47 pKa = 10.96 RR48 pKa = 11.84 VEE50 pKa = 4.82 CIVKK54 pKa = 9.43 PVFGNEE60 pKa = 3.67 PSNPPNYY67 pKa = 8.66 WNHH70 pKa = 5.59 SSALSGSALTDD81 pKa = 3.85 GEE83 pKa = 4.57 LHH85 pKa = 6.32 LCYY88 pKa = 10.78 SLMEE92 pKa = 4.13 VQLPEE97 pKa = 3.9 ISEE100 pKa = 4.18 QCFEE104 pKa = 4.38 DD105 pKa = 5.48 AMIVWEE111 pKa = 4.43 CFRR114 pKa = 11.84 MEE116 pKa = 4.35 TEE118 pKa = 4.63 LLMTPKK124 pKa = 9.22 ITVGGVNSSGGINSVQGTQMYY145 pKa = 8.32 FWAVGGSPLDD155 pKa = 3.56 VMYY158 pKa = 10.89 LLPKK162 pKa = 10.36 EE163 pKa = 4.11 NMRR166 pKa = 11.84 PRR168 pKa = 11.84 GNLTAFDD175 pKa = 4.45 SGHH178 pKa = 5.59 SVYY181 pKa = 10.71 DD182 pKa = 3.01 IGVQKK187 pKa = 10.76 GQVVNEE193 pKa = 4.26 TFPVEE198 pKa = 4.05 YY199 pKa = 9.75 WVADD203 pKa = 3.64 PSKK206 pKa = 11.08 NDD208 pKa = 2.79 NCRR211 pKa = 11.84 YY212 pKa = 8.68 FGRR215 pKa = 11.84 VVGGAATPPVITYY228 pKa = 9.9 SNSSTIPLLDD238 pKa = 3.81 EE239 pKa = 4.58 NGLGIMCNNGRR250 pKa = 11.84 CYY252 pKa = 8.99 ITCADD257 pKa = 3.16 ITGYY261 pKa = 9.99 LANRR265 pKa = 11.84 VEE267 pKa = 4.41 SSHH270 pKa = 6.24 GRR272 pKa = 11.84 FFRR275 pKa = 11.84 LHH277 pKa = 5.05 FRR279 pKa = 11.84 QRR281 pKa = 11.84 RR282 pKa = 11.84 IKK284 pKa = 10.75 NPYY287 pKa = 8.41 TMNLLYY293 pKa = 10.69 KK294 pKa = 10.33 QVFQSNATAVEE305 pKa = 3.98 AQKK308 pKa = 10.97 EE309 pKa = 4.54 VVEE312 pKa = 4.42 VTTVEE317 pKa = 4.35 DD318 pKa = 3.96 EE319 pKa = 4.43 SGLPMQVSNPRR330 pKa = 11.84 CGQYY334 pKa = 9.99 TPIQQQQASPLYY346 pKa = 9.66 NVQGG350 pKa = 3.52
Molecular weight: 38.86 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.81
IPC2_protein 5.995
IPC_protein 6.033
Toseland 6.198
ProMoST 6.402
Dawson 6.338
Bjellqvist 6.326
Wikipedia 6.338
Rodwell 6.326
Grimsley 6.262
Solomon 6.338
Lehninger 6.338
Nozaki 6.605
DTASelect 6.766
Thurlkill 6.781
EMBOSS 6.751
Sillero 6.722
Patrickios 3.617
IPC_peptide 6.364
IPC2_peptide 6.649
IPC2.peptide.svr19 6.616
Protein with the highest isoelectric point:
>tr|A0A343W7C6|A0A343W7C6_9POLY VP1 protein OS=Akodon montensis polyomavirus OX=2163422 PE=3 SV=1
MM1 pKa = 7.72 ALQVLPEE8 pKa = 4.1 VPLGGGIPGVPDD20 pKa = 3.45 WLFDD24 pKa = 4.46 IIPAGLSTLQEE35 pKa = 4.11 VFHH38 pKa = 7.15 RR39 pKa = 11.84 IATGIITSYY48 pKa = 9.54 YY49 pKa = 7.22 HH50 pKa = 5.81 TGRR53 pKa = 11.84 AIVRR57 pKa = 11.84 RR58 pKa = 11.84 AISVEE63 pKa = 3.94 MQRR66 pKa = 11.84 LLSDD70 pKa = 3.46 LRR72 pKa = 11.84 DD73 pKa = 3.65 GFEE76 pKa = 4.21 TTFEE80 pKa = 4.22 ALGQGDD86 pKa = 4.33 PVEE89 pKa = 5.13 AIIDD93 pKa = 3.78 QVRR96 pKa = 11.84 AAHH99 pKa = 5.61 STMIARR105 pKa = 11.84 EE106 pKa = 3.76 RR107 pKa = 11.84 RR108 pKa = 11.84 LIEE111 pKa = 3.83 QGTPLLPNLGGQIVDD126 pKa = 4.15 TVAGAAQTVSNIVVDD141 pKa = 3.79 VTNIPMDD148 pKa = 4.66 GYY150 pKa = 11.33 NALQDD155 pKa = 3.65 GVHH158 pKa = 7.27 RR159 pKa = 11.84 LGQWVQMAGSDD170 pKa = 3.46 GGTPHH175 pKa = 6.71 YY176 pKa = 10.62 SYY178 pKa = 10.53 PPWVLYY184 pKa = 9.94 VLQEE188 pKa = 4.3 LEE190 pKa = 4.4 TPGPNISAASRR201 pKa = 11.84 QPRR204 pKa = 11.84 TLKK207 pKa = 10.6 RR208 pKa = 11.84 KK209 pKa = 9.52 SQDD212 pKa = 2.88 GLSNPRR218 pKa = 11.84 QKK220 pKa = 10.63 KK221 pKa = 9.25 ARR223 pKa = 11.84 TRR225 pKa = 11.84 KK226 pKa = 7.93 STQSGRR232 pKa = 11.84 TKK234 pKa = 7.15 TTKK237 pKa = 10.3 NIKK240 pKa = 9.14 KK241 pKa = 9.66 GRR243 pKa = 11.84 RR244 pKa = 11.84 GGLRR248 pKa = 11.84 HH249 pKa = 6.24 SATKK253 pKa = 10.47
Molecular weight: 27.62 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.188
IPC2_protein 9.341
IPC_protein 9.604
Toseland 10.116
ProMoST 9.867
Dawson 10.306
Bjellqvist 9.984
Wikipedia 10.482
Rodwell 10.584
Grimsley 10.379
Solomon 10.35
Lehninger 10.321
Nozaki 10.087
DTASelect 9.984
Thurlkill 10.16
EMBOSS 10.526
Sillero 10.218
Patrickios 10.218
IPC_peptide 10.35
IPC2_peptide 8.536
IPC2.peptide.svr19 8.526
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
1852
186
681
370.4
41.43
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.263 ± 1.71
2.7 ± 1.05
4.644 ± 0.824
6.371 ± 0.592
2.808 ± 0.587
8.207 ± 1.829
2.484 ± 0.283
5.022 ± 0.528
6.21 ± 1.679
9.017 ± 0.558
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.808 ± 0.397
4.752 ± 0.682
5.724 ± 0.303
5.022 ± 0.523
5.508 ± 0.616
5.616 ± 0.405
5.13 ± 0.888
6.695 ± 0.702
1.404 ± 0.264
3.618 ± 0.574
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here