Amino acid dipepetide frequency for Escherichia phage vB_EcoS_HdSG1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.403AlaAla: 9.403 ± 1.602
0.913AlaCys: 0.913 ± 0.228
4.245AlaAsp: 4.245 ± 0.483
5.266AlaGlu: 5.266 ± 0.768
2.633AlaPhe: 2.633 ± 0.364
5.803AlaGly: 5.803 ± 0.696
1.236AlaHis: 1.236 ± 0.332
5.642AlaIle: 5.642 ± 0.724
5.158AlaLys: 5.158 ± 0.726
5.427AlaLeu: 5.427 ± 0.739
2.687AlaMet: 2.687 ± 0.332
4.513AlaAsn: 4.513 ± 0.595
2.579AlaPro: 2.579 ± 0.315
4.997AlaGln: 4.997 ± 0.669
3.546AlaArg: 3.546 ± 0.464
4.997AlaSer: 4.997 ± 0.963
5.696AlaThr: 5.696 ± 0.777
5.051AlaVal: 5.051 ± 0.555
1.021AlaTrp: 1.021 ± 0.226
3.063AlaTyr: 3.063 ± 0.356
0.0AlaXaa: 0.0 ± 0.0
Cys
0.699CysAla: 0.699 ± 0.213
0.376CysCys: 0.376 ± 0.179
0.913CysAsp: 0.913 ± 0.229
1.075CysGlu: 1.075 ± 0.249
0.537CysPhe: 0.537 ± 0.142
1.236CysGly: 1.236 ± 0.282
0.322CysHis: 0.322 ± 0.14
1.021CysIle: 1.021 ± 0.27
1.343CysLys: 1.343 ± 0.306
0.537CysLeu: 0.537 ± 0.187
0.43CysMet: 0.43 ± 0.152
0.699CysAsn: 0.699 ± 0.245
0.591CysPro: 0.591 ± 0.175
0.322CysGln: 0.322 ± 0.136
0.43CysArg: 0.43 ± 0.161
0.699CysSer: 0.699 ± 0.178
0.913CysThr: 0.913 ± 0.296
1.075CysVal: 1.075 ± 0.253
0.269CysTrp: 0.269 ± 0.118
0.484CysTyr: 0.484 ± 0.148
0.0CysXaa: 0.0 ± 0.0
Asp
4.675AspAla: 4.675 ± 0.623
0.752AspCys: 0.752 ± 0.186
3.17AspAsp: 3.17 ± 0.422
4.084AspGlu: 4.084 ± 0.449
2.042AspPhe: 2.042 ± 0.352
5.696AspGly: 5.696 ± 0.597
1.451AspHis: 1.451 ± 0.214
4.191AspIle: 4.191 ± 0.393
3.278AspLys: 3.278 ± 0.388
4.89AspLeu: 4.89 ± 0.545
1.343AspMet: 1.343 ± 0.267
2.364AspAsn: 2.364 ± 0.346
1.719AspPro: 1.719 ± 0.321
1.504AspGln: 1.504 ± 0.261
1.988AspArg: 1.988 ± 0.27
3.009AspSer: 3.009 ± 0.427
3.493AspThr: 3.493 ± 0.473
4.137AspVal: 4.137 ± 0.511
0.699AspTrp: 0.699 ± 0.201
2.203AspTyr: 2.203 ± 0.342
0.0AspXaa: 0.0 ± 0.0
Glu
4.89GluAla: 4.89 ± 0.591
0.967GluCys: 0.967 ± 0.257
3.815GluAsp: 3.815 ± 0.47
5.105GluGlu: 5.105 ± 0.549
2.203GluPhe: 2.203 ± 0.333
4.513GluGly: 4.513 ± 0.541
0.86GluHis: 0.86 ± 0.234
3.869GluIle: 3.869 ± 0.345
3.331GluLys: 3.331 ± 0.531
5.749GluLeu: 5.749 ± 0.659
2.74GluMet: 2.74 ± 0.452
2.794GluAsn: 2.794 ± 0.355
2.149GluPro: 2.149 ± 0.431
3.815GluGln: 3.815 ± 0.535
4.621GluArg: 4.621 ± 0.455
3.869GluSer: 3.869 ± 0.457
2.902GluThr: 2.902 ± 0.439
4.46GluVal: 4.46 ± 0.578
0.699GluTrp: 0.699 ± 0.254
2.418GluTyr: 2.418 ± 0.477
0.0GluXaa: 0.0 ± 0.0
Phe
2.848PheAla: 2.848 ± 0.386
0.537PheCys: 0.537 ± 0.164
3.009PheAsp: 3.009 ± 0.421
1.773PheGlu: 1.773 ± 0.35
1.075PhePhe: 1.075 ± 0.255
2.364PheGly: 2.364 ± 0.363
0.967PheHis: 0.967 ± 0.226
2.472PheIle: 2.472 ± 0.41
2.525PheLys: 2.525 ± 0.317
1.827PheLeu: 1.827 ± 0.345
1.128PheMet: 1.128 ± 0.238
2.633PheAsn: 2.633 ± 0.397
1.504PhePro: 1.504 ± 0.287
1.128PheGln: 1.128 ± 0.23
2.149PheArg: 2.149 ± 0.39
2.149PheSer: 2.149 ± 0.344
1.666PheThr: 1.666 ± 0.319
1.773PheVal: 1.773 ± 0.387
0.269PheTrp: 0.269 ± 0.14
0.967PheTyr: 0.967 ± 0.247
0.0PheXaa: 0.0 ± 0.0
Gly
4.728GlyAla: 4.728 ± 0.668
0.913GlyCys: 0.913 ± 0.227
3.063GlyAsp: 3.063 ± 0.322
4.299GlyGlu: 4.299 ± 0.551
3.278GlyPhe: 3.278 ± 0.468
5.534GlyGly: 5.534 ± 0.769
1.719GlyHis: 1.719 ± 0.314
3.922GlyIle: 3.922 ± 0.487
4.675GlyLys: 4.675 ± 0.48
5.588GlyLeu: 5.588 ± 0.515
2.687GlyMet: 2.687 ± 0.332
2.74GlyAsn: 2.74 ± 0.433
1.719GlyPro: 1.719 ± 0.244
3.331GlyGln: 3.331 ± 0.432
3.439GlyArg: 3.439 ± 0.387
5.857GlySer: 5.857 ± 0.562
4.836GlyThr: 4.836 ± 0.607
5.642GlyVal: 5.642 ± 0.547
1.343GlyTrp: 1.343 ± 0.269
2.31GlyTyr: 2.31 ± 0.336
0.0GlyXaa: 0.0 ± 0.0
His
1.29HisAla: 1.29 ± 0.268
0.322HisCys: 0.322 ± 0.129
0.86HisAsp: 0.86 ± 0.222
1.558HisGlu: 1.558 ± 0.31
0.591HisPhe: 0.591 ± 0.232
1.29HisGly: 1.29 ± 0.28
0.484HisHis: 0.484 ± 0.17
1.128HisIle: 1.128 ± 0.238
0.913HisLys: 0.913 ± 0.244
1.397HisLeu: 1.397 ± 0.24
0.591HisMet: 0.591 ± 0.198
0.86HisAsn: 0.86 ± 0.24
0.645HisPro: 0.645 ± 0.142
1.021HisGln: 1.021 ± 0.276
0.699HisArg: 0.699 ± 0.191
1.397HisSer: 1.397 ± 0.304
1.343HisThr: 1.343 ± 0.419
1.397HisVal: 1.397 ± 0.242
0.537HisTrp: 0.537 ± 0.149
0.752HisTyr: 0.752 ± 0.228
0.0HisXaa: 0.0 ± 0.0
Ile
5.051IleAla: 5.051 ± 0.51
0.86IleCys: 0.86 ± 0.192
4.191IleAsp: 4.191 ± 0.399
3.761IleGlu: 3.761 ± 0.447
1.397IlePhe: 1.397 ± 0.277
3.869IleGly: 3.869 ± 0.699
1.021IleHis: 1.021 ± 0.192
4.03IleIle: 4.03 ± 0.474
4.406IleLys: 4.406 ± 0.492
3.869IleLeu: 3.869 ± 0.409
1.343IleMet: 1.343 ± 0.301
3.815IleAsn: 3.815 ± 0.373
2.794IlePro: 2.794 ± 0.342
2.848IleGln: 2.848 ± 0.483
3.17IleArg: 3.17 ± 0.385
3.224IleSer: 3.224 ± 0.46
3.6IleThr: 3.6 ± 0.445
3.869IleVal: 3.869 ± 0.533
0.699IleTrp: 0.699 ± 0.198
2.525IleTyr: 2.525 ± 0.347
0.0IleXaa: 0.0 ± 0.0
Lys
5.696LysAla: 5.696 ± 0.771
0.967LysCys: 0.967 ± 0.256
3.224LysAsp: 3.224 ± 0.454
4.675LysGlu: 4.675 ± 0.549
2.525LysPhe: 2.525 ± 0.472
3.385LysGly: 3.385 ± 0.402
1.29LysHis: 1.29 ± 0.239
3.869LysIle: 3.869 ± 0.389
4.03LysLys: 4.03 ± 0.491
4.46LysLeu: 4.46 ± 0.624
1.558LysMet: 1.558 ± 0.346
1.988LysAsn: 1.988 ± 0.351
2.955LysPro: 2.955 ± 0.421
2.633LysGln: 2.633 ± 0.388
3.224LysArg: 3.224 ± 0.423
3.331LysSer: 3.331 ± 0.347
3.493LysThr: 3.493 ± 0.397
4.299LysVal: 4.299 ± 0.501
1.021LysTrp: 1.021 ± 0.206
1.881LysTyr: 1.881 ± 0.35
0.0LysXaa: 0.0 ± 0.0
Leu
6.018LeuAla: 6.018 ± 0.575
1.075LeuCys: 1.075 ± 0.235
4.299LeuAsp: 4.299 ± 0.582
4.352LeuGlu: 4.352 ± 0.541
2.418LeuPhe: 2.418 ± 0.364
4.567LeuGly: 4.567 ± 0.479
1.236LeuHis: 1.236 ± 0.253
3.922LeuIle: 3.922 ± 0.498
4.406LeuLys: 4.406 ± 0.513
4.084LeuLeu: 4.084 ± 0.514
2.096LeuMet: 2.096 ± 0.369
4.406LeuAsn: 4.406 ± 0.459
4.03LeuPro: 4.03 ± 0.573
4.137LeuGln: 4.137 ± 0.543
4.46LeuArg: 4.46 ± 0.583
4.352LeuSer: 4.352 ± 0.64
5.319LeuThr: 5.319 ± 0.731
5.373LeuVal: 5.373 ± 0.465
0.752LeuTrp: 0.752 ± 0.179
2.149LeuTyr: 2.149 ± 0.353
0.0LeuXaa: 0.0 ± 0.0
Met
2.579MetAla: 2.579 ± 0.342
0.484MetCys: 0.484 ± 0.193
1.719MetAsp: 1.719 ± 0.312
2.257MetGlu: 2.257 ± 0.4
0.806MetPhe: 0.806 ± 0.239
1.719MetGly: 1.719 ± 0.292
0.645MetHis: 0.645 ± 0.18
1.343MetIle: 1.343 ± 0.214
1.666MetLys: 1.666 ± 0.332
2.579MetLeu: 2.579 ± 0.363
0.806MetMet: 0.806 ± 0.237
1.128MetAsn: 1.128 ± 0.21
0.913MetPro: 0.913 ± 0.229
1.934MetGln: 1.934 ± 0.287
1.881MetArg: 1.881 ± 0.24
2.579MetSer: 2.579 ± 0.525
1.397MetThr: 1.397 ± 0.317
1.397MetVal: 1.397 ± 0.316
0.269MetTrp: 0.269 ± 0.11
0.806MetTyr: 0.806 ± 0.208
0.0MetXaa: 0.0 ± 0.0
Asn
4.728AsnAla: 4.728 ± 0.669
0.752AsnCys: 0.752 ± 0.159
2.633AsnAsp: 2.633 ± 0.376
3.493AsnGlu: 3.493 ± 0.477
0.86AsnPhe: 0.86 ± 0.157
4.782AsnGly: 4.782 ± 0.423
0.913AsnHis: 0.913 ± 0.268
3.17AsnIle: 3.17 ± 0.362
2.848AsnLys: 2.848 ± 0.507
3.815AsnLeu: 3.815 ± 0.425
1.182AsnMet: 1.182 ± 0.232
3.116AsnAsn: 3.116 ± 0.474
1.827AsnPro: 1.827 ± 0.299
1.719AsnGln: 1.719 ± 0.306
1.934AsnArg: 1.934 ± 0.325
3.17AsnSer: 3.17 ± 0.412
2.902AsnThr: 2.902 ± 0.474
2.848AsnVal: 2.848 ± 0.425
0.752AsnTrp: 0.752 ± 0.226
2.149AsnTyr: 2.149 ± 0.403
0.0AsnXaa: 0.0 ± 0.0
Pro
2.096ProAla: 2.096 ± 0.285
0.537ProCys: 0.537 ± 0.14
2.902ProAsp: 2.902 ± 0.387
3.546ProGlu: 3.546 ± 0.393
1.988ProPhe: 1.988 ± 0.37
3.17ProGly: 3.17 ± 0.353
1.128ProHis: 1.128 ± 0.281
1.934ProIle: 1.934 ± 0.329
1.934ProLys: 1.934 ± 0.274
2.472ProLeu: 2.472 ± 0.408
1.182ProMet: 1.182 ± 0.249
1.881ProAsn: 1.881 ± 0.256
2.149ProPro: 2.149 ± 0.335
1.881ProGln: 1.881 ± 0.556
1.988ProArg: 1.988 ± 0.346
2.257ProSer: 2.257 ± 0.358
2.418ProThr: 2.418 ± 0.355
3.063ProVal: 3.063 ± 0.398
0.645ProTrp: 0.645 ± 0.179
1.343ProTyr: 1.343 ± 0.254
0.0ProXaa: 0.0 ± 0.0
Gln
4.03GlnAla: 4.03 ± 0.649
0.376GlnCys: 0.376 ± 0.142
2.31GlnAsp: 2.31 ± 0.287
3.278GlnGlu: 3.278 ± 0.392
1.397GlnPhe: 1.397 ± 0.233
2.096GlnGly: 2.096 ± 0.411
0.699GlnHis: 0.699 ± 0.23
3.116GlnIle: 3.116 ± 0.34
1.934GlnLys: 1.934 ± 0.371
4.352GlnLeu: 4.352 ± 0.427
1.343GlnMet: 1.343 ± 0.281
2.579GlnAsn: 2.579 ± 0.422
2.472GlnPro: 2.472 ± 0.755
3.707GlnGln: 3.707 ± 1.289
2.149GlnArg: 2.149 ± 0.351
2.633GlnSer: 2.633 ± 0.386
3.439GlnThr: 3.439 ± 0.423
2.579GlnVal: 2.579 ± 0.325
0.699GlnTrp: 0.699 ± 0.187
1.773GlnTyr: 1.773 ± 0.345
0.0GlnXaa: 0.0 ± 0.0
Arg
3.278ArgAla: 3.278 ± 0.457
0.752ArgCys: 0.752 ± 0.183
2.633ArgAsp: 2.633 ± 0.363
3.224ArgGlu: 3.224 ± 0.451
2.203ArgPhe: 2.203 ± 0.345
3.385ArgGly: 3.385 ± 0.356
0.86ArgHis: 0.86 ± 0.255
3.17ArgIle: 3.17 ± 0.467
3.17ArgLys: 3.17 ± 0.474
4.621ArgLeu: 4.621 ± 0.499
1.612ArgMet: 1.612 ± 0.337
2.525ArgAsn: 2.525 ± 0.447
1.988ArgPro: 1.988 ± 0.373
2.418ArgGln: 2.418 ± 0.338
2.257ArgArg: 2.257 ± 0.366
3.009ArgSer: 3.009 ± 0.417
3.063ArgThr: 3.063 ± 0.435
3.654ArgVal: 3.654 ± 0.353
0.699ArgTrp: 0.699 ± 0.209
2.096ArgTyr: 2.096 ± 0.321
0.0ArgXaa: 0.0 ± 0.0
Ser
5.749SerAla: 5.749 ± 1.025
1.075SerCys: 1.075 ± 0.233
2.902SerAsp: 2.902 ± 0.407
3.546SerGlu: 3.546 ± 0.592
2.579SerPhe: 2.579 ± 0.385
5.588SerGly: 5.588 ± 0.666
1.236SerHis: 1.236 ± 0.315
3.17SerIle: 3.17 ± 0.363
3.761SerLys: 3.761 ± 0.434
4.406SerLeu: 4.406 ± 0.516
1.881SerMet: 1.881 ± 0.317
2.149SerAsn: 2.149 ± 0.319
2.096SerPro: 2.096 ± 0.297
2.525SerGln: 2.525 ± 0.375
2.902SerArg: 2.902 ± 0.394
4.245SerSer: 4.245 ± 0.518
3.922SerThr: 3.922 ± 0.427
4.621SerVal: 4.621 ± 0.552
0.86SerTrp: 0.86 ± 0.217
2.418SerTyr: 2.418 ± 0.349
0.0SerXaa: 0.0 ± 0.0
Thr
6.125ThrAla: 6.125 ± 0.921
0.43ThrCys: 0.43 ± 0.178
3.6ThrAsp: 3.6 ± 0.365
3.224ThrGlu: 3.224 ± 0.434
2.31ThrPhe: 2.31 ± 0.39
4.621ThrGly: 4.621 ± 0.486
1.182ThrHis: 1.182 ± 0.286
3.922ThrIle: 3.922 ± 0.423
2.74ThrLys: 2.74 ± 0.449
4.782ThrLeu: 4.782 ± 0.47
1.397ThrMet: 1.397 ± 0.246
3.224ThrAsn: 3.224 ± 0.478
3.331ThrPro: 3.331 ± 0.439
2.096ThrGln: 2.096 ± 0.38
2.74ThrArg: 2.74 ± 0.423
4.137ThrSer: 4.137 ± 0.523
4.084ThrThr: 4.084 ± 0.53
5.158ThrVal: 5.158 ± 0.698
1.075ThrTrp: 1.075 ± 0.216
1.612ThrTyr: 1.612 ± 0.312
0.0ThrXaa: 0.0 ± 0.0
Val
5.803ValAla: 5.803 ± 0.528
0.913ValCys: 0.913 ± 0.217
3.922ValAsp: 3.922 ± 0.429
4.406ValGlu: 4.406 ± 0.492
2.257ValPhe: 2.257 ± 0.409
4.943ValGly: 4.943 ± 0.504
1.128ValHis: 1.128 ± 0.281
3.546ValIle: 3.546 ± 0.433
5.105ValLys: 5.105 ± 0.574
4.89ValLeu: 4.89 ± 0.47
1.558ValMet: 1.558 ± 0.305
3.6ValAsn: 3.6 ± 0.551
3.009ValPro: 3.009 ± 0.314
3.278ValGln: 3.278 ± 0.427
3.761ValArg: 3.761 ± 0.412
4.03ValSer: 4.03 ± 0.462
4.46ValThr: 4.46 ± 0.491
5.749ValVal: 5.749 ± 0.617
1.236ValTrp: 1.236 ± 0.277
2.687ValTyr: 2.687 ± 0.386
0.0ValXaa: 0.0 ± 0.0
Trp
1.504TrpAla: 1.504 ± 0.302
0.322TrpCys: 0.322 ± 0.152
1.182TrpAsp: 1.182 ± 0.215
0.699TrpGlu: 0.699 ± 0.235
0.591TrpPhe: 0.591 ± 0.141
0.806TrpGly: 0.806 ± 0.237
0.161TrpHis: 0.161 ± 0.079
0.752TrpIle: 0.752 ± 0.197
0.967TrpLys: 0.967 ± 0.231
1.29TrpLeu: 1.29 ± 0.204
0.215TrpMet: 0.215 ± 0.099
0.699TrpAsn: 0.699 ± 0.2
0.43TrpPro: 0.43 ± 0.134
0.537TrpGln: 0.537 ± 0.176
0.967TrpArg: 0.967 ± 0.231
0.752TrpSer: 0.752 ± 0.178
0.537TrpThr: 0.537 ± 0.169
1.021TrpVal: 1.021 ± 0.22
0.269TrpTrp: 0.269 ± 0.111
0.537TrpTyr: 0.537 ± 0.157
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.687TyrAla: 2.687 ± 0.4
0.645TyrCys: 0.645 ± 0.159
2.096TyrAsp: 2.096 ± 0.325
2.149TyrGlu: 2.149 ± 0.35
1.075TyrPhe: 1.075 ± 0.218
2.257TyrGly: 2.257 ± 0.331
0.537TyrHis: 0.537 ± 0.176
2.149TyrIle: 2.149 ± 0.365
2.364TyrLys: 2.364 ± 0.347
2.418TyrLeu: 2.418 ± 0.46
1.075TyrMet: 1.075 ± 0.266
1.881TyrAsn: 1.881 ± 0.323
1.612TyrPro: 1.612 ± 0.255
1.128TyrGln: 1.128 ± 0.208
2.31TyrArg: 2.31 ± 0.421
1.934TyrSer: 1.934 ± 0.372
2.31TyrThr: 2.31 ± 0.384
3.116TyrVal: 3.116 ± 0.472
0.43TyrTrp: 0.43 ± 0.136
1.236TyrTyr: 1.236 ± 0.268
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 86 proteins (18612 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski