Rana esculenta virus

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Megaviricetes; Pimascovirales; Iridoviridae; Alphairidovirinae; Ranavirus; Common midwife toad virus

Average proteome isoelectric point is 7.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 101 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A222NTT6|A0A222NTT6_9VIRU Uncharacterized protein OS=Rana esculenta virus OX=575980 GN=ORF44 PE=4 SV=1
MM1 pKa = 7.49TEE3 pKa = 4.07NGCGCSMDD11 pKa = 3.56PAGFEE16 pKa = 4.19EE17 pKa = 4.47QASILPSWWKK27 pKa = 10.39KK28 pKa = 8.85AACGAAAGALILLLVYY44 pKa = 10.09FLSRR48 pKa = 11.84EE49 pKa = 4.03

Molecular weight: 5.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A222NTV9|A0A222NTV9_9VIRU Uncharacterized protein OS=Rana esculenta virus OX=575980 GN=ORF75 PE=4 SV=1
MM1 pKa = 7.11YY2 pKa = 10.24SVRR5 pKa = 11.84NSGCSVGCSPRR16 pKa = 11.84QGASPIMFGPSLGAMLSAPVVRR38 pKa = 11.84ASSPVVRR45 pKa = 11.84ASSPVRR51 pKa = 11.84KK52 pKa = 9.67RR53 pKa = 11.84KK54 pKa = 10.18SLVKK58 pKa = 10.07QKK60 pKa = 10.6PPVKK64 pKa = 10.11RR65 pKa = 11.84SPLKK69 pKa = 10.33KK70 pKa = 9.57SSPMRR75 pKa = 11.84KK76 pKa = 9.43SPVKK80 pKa = 10.29RR81 pKa = 11.84NSPVKK86 pKa = 10.31RR87 pKa = 11.84KK88 pKa = 10.46SPVKK92 pKa = 10.1KK93 pKa = 10.23SSPVKK98 pKa = 10.2RR99 pKa = 11.84KK100 pKa = 10.43SPVKK104 pKa = 10.1KK105 pKa = 10.23SSPVKK110 pKa = 10.2RR111 pKa = 11.84KK112 pKa = 10.43SPVKK116 pKa = 10.1KK117 pKa = 10.23SSPVKK122 pKa = 10.2RR123 pKa = 11.84KK124 pKa = 10.43SPVKK128 pKa = 10.1KK129 pKa = 10.23SSPVKK134 pKa = 10.25RR135 pKa = 11.84KK136 pKa = 9.45PPVKK140 pKa = 10.01KK141 pKa = 10.4SSPVKK146 pKa = 10.2RR147 pKa = 11.84KK148 pKa = 10.43SPVKK152 pKa = 10.1KK153 pKa = 10.23SSPVKK158 pKa = 10.2RR159 pKa = 11.84KK160 pKa = 10.43SPVKK164 pKa = 10.1KK165 pKa = 10.23SSPVKK170 pKa = 10.2RR171 pKa = 11.84KK172 pKa = 10.43SPVKK176 pKa = 10.1KK177 pKa = 10.23SSPVKK182 pKa = 10.2RR183 pKa = 11.84KK184 pKa = 10.43SPVKK188 pKa = 10.1KK189 pKa = 10.23SSPVKK194 pKa = 10.25RR195 pKa = 11.84KK196 pKa = 9.45PPVKK200 pKa = 10.01KK201 pKa = 10.4SSPVKK206 pKa = 10.2RR207 pKa = 11.84KK208 pKa = 10.43SPVKK212 pKa = 10.1KK213 pKa = 10.23SSPVKK218 pKa = 10.25RR219 pKa = 11.84KK220 pKa = 9.45PPVKK224 pKa = 10.01KK225 pKa = 10.4SSPVKK230 pKa = 10.2RR231 pKa = 11.84KK232 pKa = 10.43SPVKK236 pKa = 10.1KK237 pKa = 10.23SSPVKK242 pKa = 10.04RR243 pKa = 11.84KK244 pKa = 9.92SPAKK248 pKa = 10.0RR249 pKa = 11.84RR250 pKa = 11.84QQVCFMATKK259 pKa = 10.43

Molecular weight: 28.42 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

101

0

101

29710

49

1294

294.2

32.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.886 ± 0.37

2.08 ± 0.171

5.941 ± 0.188

5.597 ± 0.23

3.325 ± 0.124

7.253 ± 0.28

2.127 ± 0.141

3.248 ± 0.14

5.547 ± 0.348

7.913 ± 0.25

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.063 ± 0.134

2.639 ± 0.136

6.52 ± 0.329

2.885 ± 0.213

7.146 ± 0.317

7.327 ± 0.227

5.658 ± 0.194

8.455 ± 0.21

1.42 ± 0.094

2.969 ± 0.134
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski