Salmonella phage templet
Average proteome isoelectric point is 6.56
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 59 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6G8RIE4|A0A6G8RIE4_9CAUD Putative UvsX-like protein OS=Salmonella phage templet OX=2713323 GN=templet_58 PE=4 SV=1
MM1 pKa = 7.13 ATIARR6 pKa = 11.84 NVSQAYY12 pKa = 8.62 TDD14 pKa = 3.55 ALRR17 pKa = 11.84 WVAVMLGKK25 pKa = 9.85 PEE27 pKa = 3.9 DD28 pKa = 3.98 TEE30 pKa = 4.63 VEE32 pKa = 4.1 FRR34 pKa = 11.84 LNMDD38 pKa = 4.31 FFLEE42 pKa = 4.2 PMTAQDD48 pKa = 3.14 RR49 pKa = 11.84 AAWMADD55 pKa = 2.7 INAGLLPATAYY66 pKa = 10.31 YY67 pKa = 10.6 AALRR71 pKa = 11.84 KK72 pKa = 10.1 AGVTDD77 pKa = 3.3 WTDD80 pKa = 3.2 ADD82 pKa = 3.6 IKK84 pKa = 11.07 DD85 pKa = 4.49 AIEE88 pKa = 4.59 DD89 pKa = 3.71 APLPLGAVTQVAGGIPQAVQQPEE112 pKa = 3.68
Molecular weight: 12.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.385
IPC2_protein 4.151
IPC_protein 4.075
Toseland 3.872
ProMoST 4.177
Dawson 4.062
Bjellqvist 4.279
Wikipedia 3.999
Rodwell 3.91
Grimsley 3.783
Solomon 4.05
Lehninger 4.012
Nozaki 4.177
DTASelect 4.406
Thurlkill 3.923
EMBOSS 4.012
Sillero 4.19
Patrickios 3.935
IPC_peptide 4.05
IPC2_peptide 4.177
IPC2.peptide.svr19 4.121
Protein with the highest isoelectric point:
>tr|A0A6G8RI67|A0A6G8RI67_9CAUD Calcineurin-like phosphoesterase superfamily domain protein OS=Salmonella phage templet OX=2713323 GN=templet_36 PE=4 SV=1
MM1 pKa = 7.52 SLATDD6 pKa = 3.71 ILKK9 pKa = 10.26 HH10 pKa = 6.11 AGVNLAPPSSAVTKK24 pKa = 10.32 KK25 pKa = 7.19 VTRR28 pKa = 11.84 VKK30 pKa = 10.59 EE31 pKa = 3.94 KK32 pKa = 10.79 VKK34 pKa = 10.47 RR35 pKa = 11.84 KK36 pKa = 8.52 PKK38 pKa = 10.04 PRR40 pKa = 11.84 VKK42 pKa = 10.0 PVNEE46 pKa = 4.14 MPDD49 pKa = 3.55 VYY51 pKa = 10.73 PRR53 pKa = 11.84 IPGVHH58 pKa = 4.6 QPKK61 pKa = 9.16 YY62 pKa = 10.53 CVGKK66 pKa = 10.16 GLWRR70 pKa = 11.84 AHH72 pKa = 6.43 SYY74 pKa = 10.54 DD75 pKa = 3.01 GKK77 pKa = 10.98 KK78 pKa = 10.05 VVNLGEE84 pKa = 4.35 FSSQARR90 pKa = 11.84 AHH92 pKa = 6.01 MAVKK96 pKa = 10.46 LYY98 pKa = 10.67 KK99 pKa = 9.81 LWRR102 pKa = 11.84 KK103 pKa = 9.46 RR104 pKa = 11.84 GYY106 pKa = 10.35 SEE108 pKa = 5.16 IPHH111 pKa = 6.33 KK112 pKa = 10.54 PSIRR116 pKa = 11.84 LYY118 pKa = 8.83 TFRR121 pKa = 6.0
Molecular weight: 13.83 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.278
IPC2_protein 9.97
IPC_protein 10.379
Toseland 10.804
ProMoST 10.467
Dawson 10.891
Bjellqvist 10.526
Wikipedia 11.038
Rodwell 11.418
Grimsley 10.935
Solomon 10.95
Lehninger 10.921
Nozaki 10.76
DTASelect 10.526
Thurlkill 10.789
EMBOSS 11.184
Sillero 10.804
Patrickios 11.14
IPC_peptide 10.95
IPC2_peptide 9.194
IPC2.peptide.svr19 8.52
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
59
0
59
12869
30
1032
218.1
23.94
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.395 ± 0.611
1.049 ± 0.137
5.983 ± 0.216
6.543 ± 0.389
3.73 ± 0.182
7.631 ± 0.255
1.64 ± 0.172
4.756 ± 0.196
5.929 ± 0.352
7.903 ± 0.341
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.58 ± 0.162
4.266 ± 0.232
3.87 ± 0.226
3.551 ± 0.244
5.688 ± 0.229
5.929 ± 0.271
6.442 ± 0.321
7.32 ± 0.324
1.461 ± 0.173
3.357 ± 0.155
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here