Mycobacterium phage Graduation

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fromanvirus; unclassified Fromanvirus

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 97 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|V5R5Y3|V5R5Y3_9CAUD Minor tail protein OS=Mycobacterium phage Graduation OX=1391432 GN=33 PE=4 SV=1
MM1 pKa = 7.61GFTLRR6 pKa = 11.84LFGIPVLSLEE16 pKa = 4.13ITGDD20 pKa = 3.64GSAEE24 pKa = 3.94EE25 pKa = 4.62YY26 pKa = 10.64INLTGGSFEE35 pKa = 4.65LAPEE39 pKa = 3.98EE40 pKa = 4.15PEE42 pKa = 3.9YY43 pKa = 11.13DD44 pKa = 3.29EE45 pKa = 5.61EE46 pKa = 4.95YY47 pKa = 10.82YY48 pKa = 10.93EE49 pKa = 4.22EE50 pKa = 4.6DD51 pKa = 3.1RR52 pKa = 11.84SGFGFGVSS60 pKa = 3.28

Molecular weight: 6.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|V5R3N8|V5R3N8_9CAUD NrdC OS=Mycobacterium phage Graduation OX=1391432 GN=55 PE=4 SV=1
MM1 pKa = 7.2SRR3 pKa = 11.84IVLRR7 pKa = 11.84SYY9 pKa = 11.33VKK11 pKa = 10.02IAEE14 pKa = 4.15ISTGCRR20 pKa = 11.84FNDD23 pKa = 3.69WEE25 pKa = 4.21WVYY28 pKa = 10.83RR29 pKa = 11.84PWMPLWFQKK38 pKa = 10.4QLIVRR43 pKa = 11.84PLWKK47 pKa = 9.5RR48 pKa = 11.84HH49 pKa = 4.42KK50 pKa = 9.68RR51 pKa = 11.84NCIRR55 pKa = 11.84AEE57 pKa = 3.64HH58 pKa = 5.88WRR60 pKa = 11.84AMDD63 pKa = 3.45

Molecular weight: 7.99 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

97

0

97

16707

24

822

172.2

18.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.906 ± 0.416

0.706 ± 0.105

6.5 ± 0.213

6.62 ± 0.254

3.077 ± 0.156

8.559 ± 0.513

1.981 ± 0.174

4.95 ± 0.214

4.13 ± 0.235

8.475 ± 0.273

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.059 ± 0.112

3.214 ± 0.162

5.483 ± 0.229

3.376 ± 0.2

6.285 ± 0.348

5.938 ± 0.255

6.47 ± 0.247

7.225 ± 0.234

2.089 ± 0.116

2.957 ± 0.171
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski