Wenling narna-like virus 1
Average proteome isoelectric point is 7.08
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KIM1|A0A1L3KIM1_9VIRU RNA-dependent RNA polymerase OS=Wenling narna-like virus 1 OX=1923501 PE=4 SV=1
MM1 pKa = 7.1 EE2 pKa = 4.43 KK3 pKa = 10.39 VYY5 pKa = 10.91 RR6 pKa = 11.84 FLYY9 pKa = 9.1 SQYY12 pKa = 10.95 GRR14 pKa = 11.84 DD15 pKa = 3.53 FRR17 pKa = 11.84 LSDD20 pKa = 3.65 RR21 pKa = 11.84 VRR23 pKa = 11.84 SIWMYY28 pKa = 10.94 FGMLGLLEE36 pKa = 5.85 KK37 pKa = 10.22 YY38 pKa = 10.28 LKK40 pKa = 10.12 WKK42 pKa = 8.75 TADD45 pKa = 3.34 LLAAVWRR52 pKa = 11.84 SEE54 pKa = 4.0 RR55 pKa = 11.84 PPPPSFLTLGLSDD68 pKa = 5.1 VPLCLLGTDD77 pKa = 3.82 FRR79 pKa = 11.84 FWKK82 pKa = 9.73 QRR84 pKa = 11.84 LRR86 pKa = 11.84 SRR88 pKa = 11.84 SPTSKK93 pKa = 10.22 HH94 pKa = 4.28 VQLAFALNQGKK105 pKa = 9.12 AAGLPVSDD113 pKa = 4.67 DD114 pKa = 3.67 FVRR117 pKa = 11.84 AAVSSCVEE125 pKa = 4.12 RR126 pKa = 11.84 LCSDD130 pKa = 3.23 EE131 pKa = 6.0 DD132 pKa = 3.47 IVEE135 pKa = 4.17 EE136 pKa = 4.24 VTVDD140 pKa = 3.32 GTTVTVADD148 pKa = 4.22 LRR150 pKa = 11.84 RR151 pKa = 11.84 EE152 pKa = 3.59 ISRR155 pKa = 11.84 TVEE158 pKa = 3.81 EE159 pKa = 4.74 IYY161 pKa = 11.01 GHH163 pKa = 5.92 GRR165 pKa = 11.84 QEE167 pKa = 4.0 RR168 pKa = 11.84 GTPGSRR174 pKa = 11.84 RR175 pKa = 11.84 AASVKK180 pKa = 10.51 SSVEE184 pKa = 3.9 SSRR187 pKa = 11.84 SRR189 pKa = 11.84 GGPHH193 pKa = 5.99 QFLSTQLSTVRR204 pKa = 11.84 GSEE207 pKa = 3.97 FLVGVAARR215 pKa = 11.84 GHH217 pKa = 6.12 RR218 pKa = 11.84 IVWIHH223 pKa = 6.63 APGHH227 pKa = 5.9 PEE229 pKa = 3.82 DD230 pKa = 4.48 WEE232 pKa = 4.24 DD233 pKa = 3.86 TLWDD237 pKa = 3.65 SRR239 pKa = 11.84 SRR241 pKa = 11.84 AITCQGDD248 pKa = 4.33 RR249 pKa = 11.84 IPAYY253 pKa = 9.31 PVGLTEE259 pKa = 4.05 PFKK262 pKa = 11.33 VRR264 pKa = 11.84 TITRR268 pKa = 11.84 GAVDD272 pKa = 4.53 PYY274 pKa = 10.56 QLARR278 pKa = 11.84 RR279 pKa = 11.84 WQPSLWKK286 pKa = 9.35 PLQRR290 pKa = 11.84 HH291 pKa = 4.42 EE292 pKa = 4.24 CSRR295 pKa = 11.84 LVGEE299 pKa = 4.59 PVSSGVITSFLGKK312 pKa = 9.86 CDD314 pKa = 3.97 PEE316 pKa = 4.81 DD317 pKa = 3.25 GRR319 pKa = 11.84 FFVSGDD325 pKa = 3.28 YY326 pKa = 10.37 EE327 pKa = 4.16 AATDD331 pKa = 3.9 YY332 pKa = 11.4 LNPEE336 pKa = 4.13 LSEE339 pKa = 4.23 HH340 pKa = 6.27 CLEE343 pKa = 4.54 EE344 pKa = 4.09 VCHH347 pKa = 6.54 RR348 pKa = 11.84 LGVPPEE354 pKa = 4.02 EE355 pKa = 3.97 TLVLLKK361 pKa = 10.47 CLPRR365 pKa = 11.84 HH366 pKa = 5.43 EE367 pKa = 5.91 LIDD370 pKa = 3.99 PVTKK374 pKa = 9.45 EE375 pKa = 3.8 TLGVQRR381 pKa = 11.84 RR382 pKa = 11.84 GQLMGSPISFPVLTILNLAFTRR404 pKa = 11.84 YY405 pKa = 9.63 SLEE408 pKa = 4.68 LGACPEE414 pKa = 4.49 NPLSTARR421 pKa = 11.84 PLDD424 pKa = 3.49 DD425 pKa = 5.76 HH426 pKa = 7.15 EE427 pKa = 4.8 ILVNGDD433 pKa = 3.49 DD434 pKa = 3.89 VLFRR438 pKa = 11.84 AHH440 pKa = 6.27 PWEE443 pKa = 3.77 YY444 pKa = 10.26 RR445 pKa = 11.84 YY446 pKa = 9.52 WKK448 pKa = 10.62 AVVALGGLRR457 pKa = 11.84 PSLGKK462 pKa = 9.61 NQVSRR467 pKa = 11.84 RR468 pKa = 11.84 YY469 pKa = 8.01 FTINSEE475 pKa = 3.84 LWHH478 pKa = 7.18 AILQPEE484 pKa = 4.55 SEE486 pKa = 4.64 LFPYY490 pKa = 9.31 TWFKK494 pKa = 10.89 SEE496 pKa = 4.24 RR497 pKa = 11.84 LRR499 pKa = 11.84 FPQMGLAFGSVKK511 pKa = 10.48 GGCSEE516 pKa = 5.8 LEE518 pKa = 4.03 EE519 pKa = 4.39 QPLFSQGAPAAASSRR534 pKa = 11.84 TSCWRR539 pKa = 11.84 EE540 pKa = 3.58 FLQDD544 pKa = 3.56 APIPSLAWDD553 pKa = 4.13 MLWDD557 pKa = 4.39 LSSSLRR563 pKa = 11.84 SQVPKK568 pKa = 10.92 GMAYY572 pKa = 10.25 CLPEE576 pKa = 3.83 WLGGLGLPLPPKK588 pKa = 9.78 GHH590 pKa = 7.15 PLRR593 pKa = 11.84 EE594 pKa = 3.7 QRR596 pKa = 11.84 MAGSASLALARR607 pKa = 11.84 YY608 pKa = 9.17 IRR610 pKa = 11.84 DD611 pKa = 3.15 NWDD614 pKa = 3.01 TRR616 pKa = 11.84 TVRR619 pKa = 11.84 SWWRR623 pKa = 11.84 GRR625 pKa = 11.84 AYY627 pKa = 10.45 GGNSPAYY634 pKa = 10.18 LEE636 pKa = 4.38 YY637 pKa = 10.81 ADD639 pKa = 4.37 EE640 pKa = 4.1 QLHH643 pKa = 6.25 RR644 pKa = 11.84 ALHH647 pKa = 4.99 SLKK650 pKa = 10.45 VLPEE654 pKa = 4.09 PVPIDD659 pKa = 3.59 QDD661 pKa = 3.4 PDD663 pKa = 3.64 RR664 pKa = 11.84 YY665 pKa = 9.74 PLPLSSFFPIGVFSYY680 pKa = 9.3 EE681 pKa = 4.12 YY682 pKa = 9.18 CTKK685 pKa = 10.71 ADD687 pKa = 3.69 KK688 pKa = 11.23 EE689 pKa = 3.9 EE690 pKa = 4.47 DD691 pKa = 3.2 MGKK694 pKa = 9.9 RR695 pKa = 11.84 LANLSKK701 pKa = 10.16 SWTRR705 pKa = 11.84 LLYY708 pKa = 10.21 RR709 pKa = 11.84 AWGSKK714 pKa = 7.3 TLPLHH719 pKa = 6.0 GSEE722 pKa = 5.28 IEE724 pKa = 4.15 YY725 pKa = 10.79 NPPKK729 pKa = 10.3 TFLSTRR735 pKa = 11.84 WITCC739 pKa = 3.57
Molecular weight: 83.73 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.075
IPC2_protein 7.424
IPC_protein 7.263
Toseland 6.956
ProMoST 7.878
Dawson 7.951
Bjellqvist 8.375
Wikipedia 7.863
Rodwell 7.966
Grimsley 7.029
Solomon 7.98
Lehninger 8.009
Nozaki 8.58
DTASelect 8.097
Thurlkill 8.141
EMBOSS 8.17
Sillero 8.478
Patrickios 4.368
IPC_peptide 7.98
IPC2_peptide 7.556
IPC2.peptide.svr19 7.451
Protein with the highest isoelectric point:
>tr|A0A1L3KIM1|A0A1L3KIM1_9VIRU RNA-dependent RNA polymerase OS=Wenling narna-like virus 1 OX=1923501 PE=4 SV=1
MM1 pKa = 7.1 EE2 pKa = 4.43 KK3 pKa = 10.39 VYY5 pKa = 10.91 RR6 pKa = 11.84 FLYY9 pKa = 9.1 SQYY12 pKa = 10.95 GRR14 pKa = 11.84 DD15 pKa = 3.53 FRR17 pKa = 11.84 LSDD20 pKa = 3.65 RR21 pKa = 11.84 VRR23 pKa = 11.84 SIWMYY28 pKa = 10.94 FGMLGLLEE36 pKa = 5.85 KK37 pKa = 10.22 YY38 pKa = 10.28 LKK40 pKa = 10.12 WKK42 pKa = 8.75 TADD45 pKa = 3.34 LLAAVWRR52 pKa = 11.84 SEE54 pKa = 4.0 RR55 pKa = 11.84 PPPPSFLTLGLSDD68 pKa = 5.1 VPLCLLGTDD77 pKa = 3.82 FRR79 pKa = 11.84 FWKK82 pKa = 9.73 QRR84 pKa = 11.84 LRR86 pKa = 11.84 SRR88 pKa = 11.84 SPTSKK93 pKa = 10.22 HH94 pKa = 4.28 VQLAFALNQGKK105 pKa = 9.12 AAGLPVSDD113 pKa = 4.67 DD114 pKa = 3.67 FVRR117 pKa = 11.84 AAVSSCVEE125 pKa = 4.12 RR126 pKa = 11.84 LCSDD130 pKa = 3.23 EE131 pKa = 6.0 DD132 pKa = 3.47 IVEE135 pKa = 4.17 EE136 pKa = 4.24 VTVDD140 pKa = 3.32 GTTVTVADD148 pKa = 4.22 LRR150 pKa = 11.84 RR151 pKa = 11.84 EE152 pKa = 3.59 ISRR155 pKa = 11.84 TVEE158 pKa = 3.81 EE159 pKa = 4.74 IYY161 pKa = 11.01 GHH163 pKa = 5.92 GRR165 pKa = 11.84 QEE167 pKa = 4.0 RR168 pKa = 11.84 GTPGSRR174 pKa = 11.84 RR175 pKa = 11.84 AASVKK180 pKa = 10.51 SSVEE184 pKa = 3.9 SSRR187 pKa = 11.84 SRR189 pKa = 11.84 GGPHH193 pKa = 5.99 QFLSTQLSTVRR204 pKa = 11.84 GSEE207 pKa = 3.97 FLVGVAARR215 pKa = 11.84 GHH217 pKa = 6.12 RR218 pKa = 11.84 IVWIHH223 pKa = 6.63 APGHH227 pKa = 5.9 PEE229 pKa = 3.82 DD230 pKa = 4.48 WEE232 pKa = 4.24 DD233 pKa = 3.86 TLWDD237 pKa = 3.65 SRR239 pKa = 11.84 SRR241 pKa = 11.84 AITCQGDD248 pKa = 4.33 RR249 pKa = 11.84 IPAYY253 pKa = 9.31 PVGLTEE259 pKa = 4.05 PFKK262 pKa = 11.33 VRR264 pKa = 11.84 TITRR268 pKa = 11.84 GAVDD272 pKa = 4.53 PYY274 pKa = 10.56 QLARR278 pKa = 11.84 RR279 pKa = 11.84 WQPSLWKK286 pKa = 9.35 PLQRR290 pKa = 11.84 HH291 pKa = 4.42 EE292 pKa = 4.24 CSRR295 pKa = 11.84 LVGEE299 pKa = 4.59 PVSSGVITSFLGKK312 pKa = 9.86 CDD314 pKa = 3.97 PEE316 pKa = 4.81 DD317 pKa = 3.25 GRR319 pKa = 11.84 FFVSGDD325 pKa = 3.28 YY326 pKa = 10.37 EE327 pKa = 4.16 AATDD331 pKa = 3.9 YY332 pKa = 11.4 LNPEE336 pKa = 4.13 LSEE339 pKa = 4.23 HH340 pKa = 6.27 CLEE343 pKa = 4.54 EE344 pKa = 4.09 VCHH347 pKa = 6.54 RR348 pKa = 11.84 LGVPPEE354 pKa = 4.02 EE355 pKa = 3.97 TLVLLKK361 pKa = 10.47 CLPRR365 pKa = 11.84 HH366 pKa = 5.43 EE367 pKa = 5.91 LIDD370 pKa = 3.99 PVTKK374 pKa = 9.45 EE375 pKa = 3.8 TLGVQRR381 pKa = 11.84 RR382 pKa = 11.84 GQLMGSPISFPVLTILNLAFTRR404 pKa = 11.84 YY405 pKa = 9.63 SLEE408 pKa = 4.68 LGACPEE414 pKa = 4.49 NPLSTARR421 pKa = 11.84 PLDD424 pKa = 3.49 DD425 pKa = 5.76 HH426 pKa = 7.15 EE427 pKa = 4.8 ILVNGDD433 pKa = 3.49 DD434 pKa = 3.89 VLFRR438 pKa = 11.84 AHH440 pKa = 6.27 PWEE443 pKa = 3.77 YY444 pKa = 10.26 RR445 pKa = 11.84 YY446 pKa = 9.52 WKK448 pKa = 10.62 AVVALGGLRR457 pKa = 11.84 PSLGKK462 pKa = 9.61 NQVSRR467 pKa = 11.84 RR468 pKa = 11.84 YY469 pKa = 8.01 FTINSEE475 pKa = 3.84 LWHH478 pKa = 7.18 AILQPEE484 pKa = 4.55 SEE486 pKa = 4.64 LFPYY490 pKa = 9.31 TWFKK494 pKa = 10.89 SEE496 pKa = 4.24 RR497 pKa = 11.84 LRR499 pKa = 11.84 FPQMGLAFGSVKK511 pKa = 10.48 GGCSEE516 pKa = 5.8 LEE518 pKa = 4.03 EE519 pKa = 4.39 QPLFSQGAPAAASSRR534 pKa = 11.84 TSCWRR539 pKa = 11.84 EE540 pKa = 3.58 FLQDD544 pKa = 3.56 APIPSLAWDD553 pKa = 4.13 MLWDD557 pKa = 4.39 LSSSLRR563 pKa = 11.84 SQVPKK568 pKa = 10.92 GMAYY572 pKa = 10.25 CLPEE576 pKa = 3.83 WLGGLGLPLPPKK588 pKa = 9.78 GHH590 pKa = 7.15 PLRR593 pKa = 11.84 EE594 pKa = 3.7 QRR596 pKa = 11.84 MAGSASLALARR607 pKa = 11.84 YY608 pKa = 9.17 IRR610 pKa = 11.84 DD611 pKa = 3.15 NWDD614 pKa = 3.01 TRR616 pKa = 11.84 TVRR619 pKa = 11.84 SWWRR623 pKa = 11.84 GRR625 pKa = 11.84 AYY627 pKa = 10.45 GGNSPAYY634 pKa = 10.18 LEE636 pKa = 4.38 YY637 pKa = 10.81 ADD639 pKa = 4.37 EE640 pKa = 4.1 QLHH643 pKa = 6.25 RR644 pKa = 11.84 ALHH647 pKa = 4.99 SLKK650 pKa = 10.45 VLPEE654 pKa = 4.09 PVPIDD659 pKa = 3.59 QDD661 pKa = 3.4 PDD663 pKa = 3.64 RR664 pKa = 11.84 YY665 pKa = 9.74 PLPLSSFFPIGVFSYY680 pKa = 9.3 EE681 pKa = 4.12 YY682 pKa = 9.18 CTKK685 pKa = 10.71 ADD687 pKa = 3.69 KK688 pKa = 11.23 EE689 pKa = 3.9 EE690 pKa = 4.47 DD691 pKa = 3.2 MGKK694 pKa = 9.9 RR695 pKa = 11.84 LANLSKK701 pKa = 10.16 SWTRR705 pKa = 11.84 LLYY708 pKa = 10.21 RR709 pKa = 11.84 AWGSKK714 pKa = 7.3 TLPLHH719 pKa = 6.0 GSEE722 pKa = 5.28 IEE724 pKa = 4.15 YY725 pKa = 10.79 NPPKK729 pKa = 10.3 TFLSTRR735 pKa = 11.84 WITCC739 pKa = 3.57
Molecular weight: 83.73 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.075
IPC2_protein 7.424
IPC_protein 7.263
Toseland 6.956
ProMoST 7.878
Dawson 7.951
Bjellqvist 8.375
Wikipedia 7.863
Rodwell 7.966
Grimsley 7.029
Solomon 7.98
Lehninger 8.009
Nozaki 8.58
DTASelect 8.097
Thurlkill 8.141
EMBOSS 8.17
Sillero 8.478
Patrickios 4.368
IPC_peptide 7.98
IPC2_peptide 7.556
IPC2.peptide.svr19 7.451
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
739
739
739
739.0
83.73
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.36 ± 0.0
2.03 ± 0.0
4.871 ± 0.0
6.766 ± 0.0
3.789 ± 0.0
7.172 ± 0.0
2.3 ± 0.0
2.977 ± 0.0
3.518 ± 0.0
11.773 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.218 ± 0.0
1.488 ± 0.0
7.442 ± 0.0
3.112 ± 0.0
8.796 ± 0.0
8.931 ± 0.0
4.871 ± 0.0
6.089 ± 0.0
3.112 ± 0.0
3.383 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here