Apple geminivirus PL-2015

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; unclassified Geminiviridae

Average proteome isoelectric point is 7.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0D5W451|A0A0D5W451_9GEMI Transcriptional activator protein OS=Apple geminivirus PL-2015 OX=1633752 GN=C2 PE=3 SV=1
MM1 pKa = 7.2EE2 pKa = 5.38AVFNHH7 pKa = 6.21LVGKK11 pKa = 10.07KK12 pKa = 9.14LQPKK16 pKa = 9.78DD17 pKa = 3.53VLPAITAWICEE28 pKa = 4.26KK29 pKa = 10.59GTGQQLIDD37 pKa = 3.65WVNGLEE43 pKa = 4.16GEE45 pKa = 4.25KK46 pKa = 10.39EE47 pKa = 4.14AIFRR51 pKa = 11.84ALNSCSAEE59 pKa = 3.88QEE61 pKa = 4.49GEE63 pKa = 3.97MEE65 pKa = 4.6ANDD68 pKa = 4.49DD69 pKa = 4.2GGSHH73 pKa = 6.01EE74 pKa = 4.44ASPVRR79 pKa = 11.84EE80 pKa = 4.31CVPAEE85 pKa = 4.07PNEE88 pKa = 4.14GAANRR93 pKa = 11.84IISISS98 pKa = 3.28

Molecular weight: 10.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0D5W3M0|A0A0D5W3M0_9GEMI Replication enhancer OS=Apple geminivirus PL-2015 OX=1633752 GN=C3 PE=3 SV=1
MM1 pKa = 7.59GSLISTCLSNSKK13 pKa = 10.55GSTHH17 pKa = 6.68ARR19 pKa = 11.84INDD22 pKa = 3.13SSTWYY27 pKa = 8.43PQPDD31 pKa = 3.36QHH33 pKa = 9.02ISIQTYY39 pKa = 8.66RR40 pKa = 11.84EE41 pKa = 3.55LRR43 pKa = 11.84ARR45 pKa = 11.84PMSRR49 pKa = 11.84PILRR53 pKa = 11.84RR54 pKa = 11.84EE55 pKa = 4.16GNFLTMEE62 pKa = 5.18FSRR65 pKa = 11.84SMPEE69 pKa = 3.85VQGGRR74 pKa = 11.84ASII77 pKa = 3.9

Molecular weight: 8.74 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1082

77

352

180.3

20.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.377 ± 0.72

2.126 ± 0.712

4.529 ± 0.343

6.377 ± 1.073

4.159 ± 0.748

5.638 ± 0.936

3.142 ± 0.205

5.083 ± 0.801

5.638 ± 0.559

8.226 ± 0.324

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.218 ± 0.636

5.268 ± 0.304

6.1 ± 0.995

3.789 ± 0.229

6.654 ± 1.05

8.965 ± 0.752

5.083 ± 0.738

5.453 ± 0.521

1.571 ± 0.243

3.604 ± 0.497
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski