Amino acid dipepetide frequency for Cyanophage S-SSM6a

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.831AlaAla: 4.831 ± 0.347
0.538AlaCys: 0.538 ± 0.096
3.472AlaAsp: 3.472 ± 0.249
3.458AlaGlu: 3.458 ± 0.256
2.543AlaPhe: 2.543 ± 0.203
5.409AlaGly: 5.409 ± 0.383
1.13AlaHis: 1.13 ± 0.133
3.633AlaIle: 3.633 ± 0.203
3.956AlaLys: 3.956 ± 0.328
4.454AlaLeu: 4.454 ± 0.26
1.453AlaMet: 1.453 ± 0.148
3.62AlaAsn: 3.62 ± 0.262
2.086AlaPro: 2.086 ± 0.162
2.126AlaGln: 2.126 ± 0.218
2.314AlaArg: 2.314 ± 0.188
4.158AlaSer: 4.158 ± 0.331
5.127AlaThr: 5.127 ± 0.481
3.848AlaVal: 3.848 ± 0.261
0.646AlaTrp: 0.646 ± 0.107
2.314AlaTyr: 2.314 ± 0.189
0.0AlaXaa: 0.0 ± 0.0
Cys
0.834CysAla: 0.834 ± 0.126
0.35CysCys: 0.35 ± 0.122
0.754CysAsp: 0.754 ± 0.099
0.673CysGlu: 0.673 ± 0.112
0.525CysPhe: 0.525 ± 0.098
0.767CysGly: 0.767 ± 0.124
0.283CysHis: 0.283 ± 0.066
0.673CysIle: 0.673 ± 0.107
0.659CysLys: 0.659 ± 0.112
0.673CysLeu: 0.673 ± 0.112
0.323CysMet: 0.323 ± 0.064
0.538CysAsn: 0.538 ± 0.111
0.498CysPro: 0.498 ± 0.086
0.35CysGln: 0.35 ± 0.074
0.619CysArg: 0.619 ± 0.106
0.659CysSer: 0.659 ± 0.117
0.875CysThr: 0.875 ± 0.123
0.592CysVal: 0.592 ± 0.087
0.148CysTrp: 0.148 ± 0.054
0.525CysTyr: 0.525 ± 0.107
0.0CysXaa: 0.0 ± 0.0
Asp
4.118AspAla: 4.118 ± 0.211
0.888AspCys: 0.888 ± 0.126
4.414AspAsp: 4.414 ± 0.332
4.494AspGlu: 4.494 ± 0.297
3.418AspPhe: 3.418 ± 0.246
4.938AspGly: 4.938 ± 0.298
1.023AspHis: 1.023 ± 0.111
4.333AspIle: 4.333 ± 0.248
4.252AspLys: 4.252 ± 0.314
5.49AspLeu: 5.49 ± 0.256
1.736AspMet: 1.736 ± 0.209
3.404AspAsn: 3.404 ± 0.179
3.512AspPro: 3.512 ± 0.272
2.153AspGln: 2.153 ± 0.173
2.301AspArg: 2.301 ± 0.184
3.889AspSer: 3.889 ± 0.223
3.714AspThr: 3.714 ± 0.245
4.602AspVal: 4.602 ± 0.25
1.278AspTrp: 1.278 ± 0.159
3.835AspTyr: 3.835 ± 0.244
0.0AspXaa: 0.0 ± 0.0
Glu
3.552GluAla: 3.552 ± 0.292
0.754GluCys: 0.754 ± 0.114
4.467GluAsp: 4.467 ± 0.29
5.046GluGlu: 5.046 ± 0.443
2.92GluPhe: 2.92 ± 0.186
3.996GluGly: 3.996 ± 0.275
1.251GluHis: 1.251 ± 0.141
4.467GluIle: 4.467 ± 0.322
4.642GluLys: 4.642 ± 0.41
4.965GluLeu: 4.965 ± 0.394
1.319GluMet: 1.319 ± 0.186
3.364GluAsn: 3.364 ± 0.213
2.032GluPro: 2.032 ± 0.207
2.368GluGln: 2.368 ± 0.204
2.664GluArg: 2.664 ± 0.226
3.97GluSer: 3.97 ± 0.346
3.122GluThr: 3.122 ± 0.233
4.333GluVal: 4.333 ± 0.243
0.875GluTrp: 0.875 ± 0.115
2.893GluTyr: 2.893 ± 0.225
0.0GluXaa: 0.0 ± 0.0
Phe
2.987PheAla: 2.987 ± 0.228
0.39PheCys: 0.39 ± 0.082
3.727PheAsp: 3.727 ± 0.266
2.88PheGlu: 2.88 ± 0.184
1.776PhePhe: 1.776 ± 0.14
3.068PheGly: 3.068 ± 0.229
0.794PheHis: 0.794 ± 0.104
2.53PheIle: 2.53 ± 0.193
2.826PheLys: 2.826 ± 0.238
2.826PheLeu: 2.826 ± 0.192
0.794PheMet: 0.794 ± 0.121
2.853PheAsn: 2.853 ± 0.221
1.803PhePro: 1.803 ± 0.17
1.695PheGln: 1.695 ± 0.171
1.547PheArg: 1.547 ± 0.157
3.041PheSer: 3.041 ± 0.256
3.31PheThr: 3.31 ± 0.326
3.068PheVal: 3.068 ± 0.239
0.659PheTrp: 0.659 ± 0.102
1.83PheTyr: 1.83 ± 0.167
0.0PheXaa: 0.0 ± 0.0
Gly
5.127GlyAla: 5.127 ± 0.402
0.834GlyCys: 0.834 ± 0.164
5.167GlyAsp: 5.167 ± 0.322
3.66GlyGlu: 3.66 ± 0.241
3.162GlyPhe: 3.162 ± 0.27
7.616GlyGly: 7.616 ± 0.861
1.13GlyHis: 1.13 ± 0.128
5.477GlyIle: 5.477 ± 0.626
4.239GlyLys: 4.239 ± 0.326
5.059GlyLeu: 5.059 ± 0.286
1.924GlyMet: 1.924 ± 0.229
4.548GlyAsn: 4.548 ± 0.33
1.763GlyPro: 1.763 ± 0.153
2.664GlyGln: 2.664 ± 0.17
3.108GlyArg: 3.108 ± 0.246
5.853GlySer: 5.853 ± 0.487
6.647GlyThr: 6.647 ± 0.604
5.692GlyVal: 5.692 ± 0.474
1.09GlyTrp: 1.09 ± 0.135
3.351GlyTyr: 3.351 ± 0.277
0.0GlyXaa: 0.0 ± 0.0
His
0.996HisAla: 0.996 ± 0.126
0.242HisCys: 0.242 ± 0.067
1.211HisAsp: 1.211 ± 0.152
0.996HisGlu: 0.996 ± 0.142
0.807HisPhe: 0.807 ± 0.124
1.319HisGly: 1.319 ± 0.155
0.552HisHis: 0.552 ± 0.122
1.184HisIle: 1.184 ± 0.142
1.144HisLys: 1.144 ± 0.158
1.184HisLeu: 1.184 ± 0.146
0.35HisMet: 0.35 ± 0.076
0.955HisAsn: 0.955 ± 0.128
1.05HisPro: 1.05 ± 0.138
0.552HisGln: 0.552 ± 0.093
0.767HisArg: 0.767 ± 0.117
1.009HisSer: 1.009 ± 0.126
1.184HisThr: 1.184 ± 0.165
1.063HisVal: 1.063 ± 0.11
0.336HisTrp: 0.336 ± 0.075
1.009HisTyr: 1.009 ± 0.145
0.0HisXaa: 0.0 ± 0.0
Ile
4.198IleAla: 4.198 ± 0.257
0.619IleCys: 0.619 ± 0.103
5.113IleAsp: 5.113 ± 0.278
4.279IleGlu: 4.279 ± 0.243
2.328IlePhe: 2.328 ± 0.196
4.723IleGly: 4.723 ± 0.412
1.036IleHis: 1.036 ± 0.16
3.593IleIle: 3.593 ± 0.284
4.266IleLys: 4.266 ± 0.286
4.481IleLeu: 4.481 ± 0.271
1.561IleMet: 1.561 ± 0.143
3.687IleAsn: 3.687 ± 0.264
2.651IlePro: 2.651 ± 0.223
2.207IleGln: 2.207 ± 0.157
2.664IleArg: 2.664 ± 0.225
4.44IleSer: 4.44 ± 0.35
5.329IleThr: 5.329 ± 0.511
3.902IleVal: 3.902 ± 0.214
0.538IleTrp: 0.538 ± 0.092
2.476IleTyr: 2.476 ± 0.206
0.0IleXaa: 0.0 ± 0.0
Lys
3.552LysAla: 3.552 ± 0.302
0.673LysCys: 0.673 ± 0.095
3.996LysAsp: 3.996 ± 0.281
4.844LysGlu: 4.844 ± 0.417
2.597LysPhe: 2.597 ± 0.208
3.7LysGly: 3.7 ± 0.383
1.198LysHis: 1.198 ± 0.17
4.387LysIle: 4.387 ± 0.315
5.732LysLys: 5.732 ± 0.782
4.952LysLeu: 4.952 ± 0.378
1.736LysMet: 1.736 ± 0.209
3.66LysAsn: 3.66 ± 0.264
2.826LysPro: 2.826 ± 0.283
2.274LysGln: 2.274 ± 0.227
2.247LysArg: 2.247 ± 0.22
4.225LysSer: 4.225 ± 0.29
3.754LysThr: 3.754 ± 0.255
4.938LysVal: 4.938 ± 0.34
0.767LysTrp: 0.767 ± 0.114
3.418LysTyr: 3.418 ± 0.304
0.0LysXaa: 0.0 ± 0.0
Leu
3.862LeuAla: 3.862 ± 0.222
0.78LeuCys: 0.78 ± 0.118
5.221LeuAsp: 5.221 ± 0.321
4.992LeuGlu: 4.992 ± 0.298
3.041LeuPhe: 3.041 ± 0.21
4.696LeuGly: 4.696 ± 0.279
1.521LeuHis: 1.521 ± 0.191
3.808LeuIle: 3.808 ± 0.246
5.356LeuLys: 5.356 ± 0.356
4.346LeuLeu: 4.346 ± 0.258
1.763LeuMet: 1.763 ± 0.171
4.481LeuAsn: 4.481 ± 0.284
3.028LeuPro: 3.028 ± 0.231
2.597LeuGln: 2.597 ± 0.204
2.933LeuArg: 2.933 ± 0.208
5.302LeuSer: 5.302 ± 0.248
4.79LeuThr: 4.79 ± 0.298
4.589LeuVal: 4.589 ± 0.282
0.686LeuTrp: 0.686 ± 0.129
2.705LeuTyr: 2.705 ± 0.216
0.0LeuXaa: 0.0 ± 0.0
Met
1.534MetAla: 1.534 ± 0.161
0.188MetCys: 0.188 ± 0.055
1.278MetAsp: 1.278 ± 0.161
1.319MetGlu: 1.319 ± 0.179
0.982MetPhe: 0.982 ± 0.128
1.238MetGly: 1.238 ± 0.152
0.296MetHis: 0.296 ± 0.08
1.373MetIle: 1.373 ± 0.148
1.884MetLys: 1.884 ± 0.211
1.426MetLeu: 1.426 ± 0.176
0.7MetMet: 0.7 ± 0.1
1.346MetAsn: 1.346 ± 0.161
1.238MetPro: 1.238 ± 0.192
0.754MetGln: 0.754 ± 0.114
1.009MetArg: 1.009 ± 0.119
1.776MetSer: 1.776 ± 0.201
1.83MetThr: 1.83 ± 0.165
1.117MetVal: 1.117 ± 0.128
0.229MetTrp: 0.229 ± 0.059
0.767MetTyr: 0.767 ± 0.109
0.0MetXaa: 0.0 ± 0.0
Asn
3.27AsnAla: 3.27 ± 0.276
0.606AsnCys: 0.606 ± 0.108
3.7AsnAsp: 3.7 ± 0.217
3.229AsnGlu: 3.229 ± 0.238
2.651AsnPhe: 2.651 ± 0.213
4.198AsnGly: 4.198 ± 0.295
1.063AsnHis: 1.063 ± 0.118
4.225AsnIle: 4.225 ± 0.282
3.162AsnLys: 3.162 ± 0.254
4.602AsnLeu: 4.602 ± 0.287
1.076AsnMet: 1.076 ± 0.128
3.149AsnAsn: 3.149 ± 0.303
2.88AsnPro: 2.88 ± 0.182
1.951AsnGln: 1.951 ± 0.16
2.234AsnArg: 2.234 ± 0.149
3.525AsnSer: 3.525 ± 0.244
3.983AsnThr: 3.983 ± 0.284
4.225AsnVal: 4.225 ± 0.41
0.78AsnTrp: 0.78 ± 0.115
2.409AsnTyr: 2.409 ± 0.197
0.0AsnXaa: 0.0 ± 0.0
Pro
2.032ProAla: 2.032 ± 0.146
0.431ProCys: 0.431 ± 0.091
3.081ProAsp: 3.081 ± 0.258
3.351ProGlu: 3.351 ± 0.35
1.857ProPhe: 1.857 ± 0.157
2.624ProGly: 2.624 ± 0.208
0.915ProHis: 0.915 ± 0.13
2.341ProIle: 2.341 ± 0.187
2.449ProLys: 2.449 ± 0.255
2.368ProLeu: 2.368 ± 0.142
0.861ProMet: 0.861 ± 0.125
2.449ProAsn: 2.449 ± 0.183
1.736ProPro: 1.736 ± 0.205
1.601ProGln: 1.601 ± 0.146
1.588ProArg: 1.588 ± 0.21
3.028ProSer: 3.028 ± 0.214
3.162ProThr: 3.162 ± 0.199
2.785ProVal: 2.785 ± 0.206
0.579ProTrp: 0.579 ± 0.109
1.534ProTyr: 1.534 ± 0.164
0.0ProXaa: 0.0 ± 0.0
Gln
1.547GlnAla: 1.547 ± 0.134
0.377GlnCys: 0.377 ± 0.073
1.978GlnAsp: 1.978 ± 0.182
2.328GlnGlu: 2.328 ± 0.186
2.18GlnPhe: 2.18 ± 0.181
2.503GlnGly: 2.503 ± 0.21
0.619GlnHis: 0.619 ± 0.096
2.489GlnIle: 2.489 ± 0.188
2.516GlnLys: 2.516 ± 0.234
2.557GlnLeu: 2.557 ± 0.213
0.969GlnMet: 0.969 ± 0.135
1.44GlnAsn: 1.44 ± 0.139
1.144GlnPro: 1.144 ± 0.124
1.642GlnGln: 1.642 ± 0.161
1.615GlnArg: 1.615 ± 0.128
2.476GlnSer: 2.476 ± 0.201
2.476GlnThr: 2.476 ± 0.199
2.314GlnVal: 2.314 ± 0.152
0.606GlnTrp: 0.606 ± 0.1
1.574GlnTyr: 1.574 ± 0.155
0.0GlnXaa: 0.0 ± 0.0
Arg
2.288ArgAla: 2.288 ± 0.209
0.673ArgCys: 0.673 ± 0.125
2.597ArgAsp: 2.597 ± 0.176
2.18ArgGlu: 2.18 ± 0.216
1.897ArgPhe: 1.897 ± 0.16
3.216ArgGly: 3.216 ± 0.233
0.754ArgHis: 0.754 ± 0.096
2.718ArgIle: 2.718 ± 0.195
3.014ArgLys: 3.014 ± 0.258
3.135ArgLeu: 3.135 ± 0.218
0.996ArgMet: 0.996 ± 0.121
2.099ArgAsn: 2.099 ± 0.185
1.467ArgPro: 1.467 ± 0.172
1.171ArgGln: 1.171 ± 0.147
1.722ArgArg: 1.722 ± 0.19
2.476ArgSer: 2.476 ± 0.19
2.018ArgThr: 2.018 ± 0.176
2.637ArgVal: 2.637 ± 0.257
0.552ArgTrp: 0.552 ± 0.083
1.601ArgTyr: 1.601 ± 0.179
0.0ArgXaa: 0.0 ± 0.0
Ser
4.198SerAla: 4.198 ± 0.287
0.552SerCys: 0.552 ± 0.102
4.239SerAsp: 4.239 ± 0.263
3.149SerGlu: 3.149 ± 0.255
3.243SerPhe: 3.243 ± 0.222
7.535SerGly: 7.535 ± 0.837
1.359SerHis: 1.359 ± 0.141
4.494SerIle: 4.494 ± 0.263
3.875SerLys: 3.875 ± 0.261
4.979SerLeu: 4.979 ± 0.313
1.359SerMet: 1.359 ± 0.179
3.889SerAsn: 3.889 ± 0.281
2.449SerPro: 2.449 ± 0.17
2.422SerGln: 2.422 ± 0.207
2.489SerArg: 2.489 ± 0.145
4.898SerSer: 4.898 ± 0.381
4.898SerThr: 4.898 ± 0.573
4.414SerVal: 4.414 ± 0.28
0.713SerTrp: 0.713 ± 0.103
2.907SerTyr: 2.907 ± 0.203
0.0SerXaa: 0.0 ± 0.0
Thr
5.207ThrAla: 5.207 ± 0.441
0.713ThrCys: 0.713 ± 0.115
4.118ThrAsp: 4.118 ± 0.239
3.929ThrGlu: 3.929 ± 0.235
3.606ThrPhe: 3.606 ± 0.404
6.338ThrGly: 6.338 ± 0.695
0.902ThrHis: 0.902 ± 0.12
4.817ThrIle: 4.817 ± 0.39
3.916ThrLys: 3.916 ± 0.266
4.817ThrLeu: 4.817 ± 0.271
0.996ThrMet: 0.996 ± 0.136
3.741ThrAsn: 3.741 ± 0.318
3.364ThrPro: 3.364 ± 0.244
2.193ThrGln: 2.193 ± 0.193
2.18ThrArg: 2.18 ± 0.174
5.248ThrSer: 5.248 ± 0.506
5.477ThrThr: 5.477 ± 0.676
5.221ThrVal: 5.221 ± 0.449
0.821ThrTrp: 0.821 ± 0.088
2.705ThrTyr: 2.705 ± 0.242
0.0ThrXaa: 0.0 ± 0.0
Val
3.848ValAla: 3.848 ± 0.276
0.78ValCys: 0.78 ± 0.099
4.804ValAsp: 4.804 ± 0.22
4.508ValGlu: 4.508 ± 0.285
2.691ValPhe: 2.691 ± 0.225
6.244ValGly: 6.244 ± 0.686
0.902ValHis: 0.902 ± 0.12
4.306ValIle: 4.306 ± 0.287
4.4ValLys: 4.4 ± 0.262
3.996ValLeu: 3.996 ± 0.243
1.198ValMet: 1.198 ± 0.161
4.118ValAsn: 4.118 ± 0.267
2.758ValPro: 2.758 ± 0.179
2.355ValGln: 2.355 ± 0.181
2.503ValArg: 2.503 ± 0.186
4.79ValSer: 4.79 ± 0.33
5.369ValThr: 5.369 ± 0.45
4.629ValVal: 4.629 ± 0.303
0.632ValTrp: 0.632 ± 0.087
2.584ValTyr: 2.584 ± 0.186
0.0ValXaa: 0.0 ± 0.0
Trp
0.74TrpAla: 0.74 ± 0.094
0.148TrpCys: 0.148 ± 0.045
0.902TrpAsp: 0.902 ± 0.131
0.915TrpGlu: 0.915 ± 0.103
0.552TrpPhe: 0.552 ± 0.099
0.928TrpGly: 0.928 ± 0.113
0.336TrpHis: 0.336 ± 0.07
0.928TrpIle: 0.928 ± 0.119
0.659TrpLys: 0.659 ± 0.096
0.834TrpLeu: 0.834 ± 0.144
0.431TrpMet: 0.431 ± 0.074
0.794TrpAsn: 0.794 ± 0.118
0.309TrpPro: 0.309 ± 0.061
0.498TrpGln: 0.498 ± 0.093
0.713TrpArg: 0.713 ± 0.12
0.848TrpSer: 0.848 ± 0.121
0.673TrpThr: 0.673 ± 0.086
0.686TrpVal: 0.686 ± 0.094
0.188TrpTrp: 0.188 ± 0.053
0.552TrpTyr: 0.552 ± 0.09
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.395TyrAla: 2.395 ± 0.191
0.78TyrCys: 0.78 ± 0.112
3.552TyrAsp: 3.552 ± 0.21
2.772TyrGlu: 2.772 ± 0.188
1.628TyrPhe: 1.628 ± 0.151
3.055TyrGly: 3.055 ± 0.195
0.848TyrHis: 0.848 ± 0.142
2.314TyrIle: 2.314 ± 0.165
2.543TyrLys: 2.543 ± 0.224
3.351TyrLeu: 3.351 ± 0.252
0.727TyrMet: 0.727 ± 0.118
2.785TyrAsn: 2.785 ± 0.209
2.18TyrPro: 2.18 ± 0.201
1.776TyrGln: 1.776 ± 0.149
1.991TyrArg: 1.991 ± 0.197
2.449TyrSer: 2.449 ± 0.166
2.57TyrThr: 2.57 ± 0.219
2.758TyrVal: 2.758 ± 0.195
0.511TyrTrp: 0.511 ± 0.093
2.341TyrTyr: 2.341 ± 0.174
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 304 proteins (74317 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski