Inmirania thermothiophila

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Chromatiales; Ectothiorhodospiraceae; Inmirania

Average proteome isoelectric point is 7.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2472 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3N1Y9V9|A0A3N1Y9V9_9GAMM Uncharacterized protein OS=Inmirania thermothiophila OX=1750597 GN=EDC57_0308 PE=4 SV=1
MM1 pKa = 7.42AFVVLEE7 pKa = 3.67NCIRR11 pKa = 11.84CKK13 pKa = 10.3YY14 pKa = 7.78TDD16 pKa = 4.19CVDD19 pKa = 3.61VCPVDD24 pKa = 4.48CFHH27 pKa = 7.48EE28 pKa = 5.32GPNMLVIDD36 pKa = 4.45PEE38 pKa = 4.17EE39 pKa = 5.67CIDD42 pKa = 4.28CALCEE47 pKa = 4.23PEE49 pKa = 5.17CPAEE53 pKa = 4.53AIVPEE58 pKa = 4.49DD59 pKa = 4.62EE60 pKa = 4.35VPEE63 pKa = 4.17DD64 pKa = 3.34QKK66 pKa = 11.81EE67 pKa = 4.23FIQLNAEE74 pKa = 4.19LAKK77 pKa = 10.03VWPVLSEE84 pKa = 4.31KK85 pKa = 10.49KK86 pKa = 10.5DD87 pKa = 3.76PLPDD91 pKa = 4.04ADD93 pKa = 3.85EE94 pKa = 4.45WNGVAGKK101 pKa = 9.71RR102 pKa = 11.84QYY104 pKa = 11.25LEE106 pKa = 3.86RR107 pKa = 5.9

Molecular weight: 12.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3N1Y6H8|A0A3N1Y6H8_9GAMM C(7)-type cytochrome triheme protein OS=Inmirania thermothiophila OX=1750597 GN=EDC57_2132 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNIRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.2RR14 pKa = 11.84THH16 pKa = 6.01GFRR19 pKa = 11.84ARR21 pKa = 11.84MRR23 pKa = 11.84TRR25 pKa = 11.84AGRR28 pKa = 11.84RR29 pKa = 11.84VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.22GRR39 pKa = 11.84ARR41 pKa = 11.84LTPP44 pKa = 3.93

Molecular weight: 5.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2472

0

2472

817919

26

1827

330.9

35.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.951 ± 0.098

0.94 ± 0.019

4.852 ± 0.037

7.12 ± 0.049

2.877 ± 0.03

9.411 ± 0.043

2.224 ± 0.024

3.521 ± 0.035

1.608 ± 0.038

11.514 ± 0.064

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.826 ± 0.022

1.55 ± 0.024

5.921 ± 0.035

2.295 ± 0.022

10.338 ± 0.058

3.152 ± 0.027

4.046 ± 0.034

8.333 ± 0.045

1.412 ± 0.02

2.107 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski