Streptococcus satellite phage Javan230

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 7.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 16 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZHW8|A0A4D5ZHW8_9VIRU DNA replication protein OS=Streptococcus satellite phage Javan230 OX=2558583 GN=JavanS230_0008 PE=4 SV=1
MM1 pKa = 7.38TDD3 pKa = 2.67KK4 pKa = 11.0TGANLAKK11 pKa = 10.34VRR13 pKa = 11.84AEE15 pKa = 3.88KK16 pKa = 10.47FGEE19 pKa = 4.03NLSEE23 pKa = 4.14IFDD26 pKa = 3.39IMVEE30 pKa = 4.24FEE32 pKa = 4.65LEE34 pKa = 4.33GKK36 pKa = 9.65FDD38 pKa = 4.39CYY40 pKa = 10.99NTTDD44 pKa = 3.65YY45 pKa = 11.82SKK47 pKa = 9.83MARR50 pKa = 11.84VLEE53 pKa = 4.05ILTDD57 pKa = 4.46FSVMWDD63 pKa = 3.0KK64 pKa = 11.53GQIILVSKK72 pKa = 10.11EE73 pKa = 3.94SEE75 pKa = 4.14VRR77 pKa = 11.84QQ78 pKa = 3.55

Molecular weight: 9.0 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZH39|A0A4D5ZH39_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan230 OX=2558583 GN=JavanS230_0003 PE=4 SV=1
MM1 pKa = 7.53NIKK4 pKa = 9.4EE5 pKa = 4.28VKK7 pKa = 9.58KK8 pKa = 10.65KK9 pKa = 10.69NGTIVYY15 pKa = 8.98RR16 pKa = 11.84ANVYY20 pKa = 10.64LGTDD24 pKa = 2.86RR25 pKa = 11.84LTGKK29 pKa = 10.21KK30 pKa = 9.59ARR32 pKa = 11.84KK33 pKa = 9.68NITASTKK40 pKa = 10.53KK41 pKa = 10.33GVKK44 pKa = 8.38TKK46 pKa = 10.69ARR48 pKa = 11.84EE49 pKa = 4.05AVNEE53 pKa = 4.51FINNGYY59 pKa = 5.23TTKK62 pKa = 10.36TKK64 pKa = 10.06ATIKK68 pKa = 9.55TYY70 pKa = 10.94KK71 pKa = 10.1EE72 pKa = 3.99LASLWWDD79 pKa = 3.43SYY81 pKa = 11.78KK82 pKa = 10.2NTVKK86 pKa = 10.7PNTQQSTKK94 pKa = 10.72GLLKK98 pKa = 10.38VHH100 pKa = 7.05ILPVFGDD107 pKa = 3.7YY108 pKa = 11.03KK109 pKa = 10.72LDD111 pKa = 3.64KK112 pKa = 9.61LTTPIIQQQVNKK124 pKa = 9.58WADD127 pKa = 3.37KK128 pKa = 10.67ANKK131 pKa = 8.49GVKK134 pKa = 9.08GAYY137 pKa = 9.71ANYY140 pKa = 10.49NLLHH144 pKa = 6.1NVNSRR149 pKa = 11.84ILQYY153 pKa = 10.96GVTMQLIQHH162 pKa = 6.05NPARR166 pKa = 11.84DD167 pKa = 3.59VIVPRR172 pKa = 11.84KK173 pKa = 8.39KK174 pKa = 10.21QKK176 pKa = 10.48EE177 pKa = 3.82KK178 pKa = 10.63TKK180 pKa = 10.61IKK182 pKa = 10.49FLDD185 pKa = 3.72RR186 pKa = 11.84QEE188 pKa = 4.75LKK190 pKa = 10.89QFLNYY195 pKa = 10.57LNTLDD200 pKa = 3.67QSDD203 pKa = 4.5YY204 pKa = 11.2EE205 pKa = 4.47SLFDD209 pKa = 3.86FVLYY213 pKa = 10.19TFLLATGLRR222 pKa = 11.84ISEE225 pKa = 4.15ALALEE230 pKa = 4.29WSDD233 pKa = 3.01IDD235 pKa = 3.89LEE237 pKa = 4.31KK238 pKa = 11.15GIVSINKK245 pKa = 8.21TLNRR249 pKa = 11.84YY250 pKa = 8.53QEE252 pKa = 4.16VNPPKK257 pKa = 10.6SKK259 pKa = 10.71AGYY262 pKa = 9.69RR263 pKa = 11.84DD264 pKa = 3.13ISIDD268 pKa = 3.12KK269 pKa = 9.51ATILMLKK276 pKa = 9.5QYY278 pKa = 11.02KK279 pKa = 9.65NRR281 pKa = 11.84QQVQSWQLGRR291 pKa = 11.84SEE293 pKa = 4.42KK294 pKa = 10.73VVFSVFTEE302 pKa = 4.95KK303 pKa = 10.78YY304 pKa = 10.36AYY306 pKa = 10.24ACNLRR311 pKa = 11.84RR312 pKa = 11.84RR313 pKa = 11.84LDD315 pKa = 3.41KK316 pKa = 10.71HH317 pKa = 6.19FKK319 pKa = 9.45NAGVTNVSFHH329 pKa = 6.06GLRR332 pKa = 11.84HH333 pKa = 4.44THH335 pKa = 5.01ATIMLYY341 pKa = 10.66AGIQPKK347 pKa = 10.23DD348 pKa = 3.39LQHH351 pKa = 6.96RR352 pKa = 11.84LGHH355 pKa = 6.13SDD357 pKa = 3.42ISMTLNTYY365 pKa = 8.7VHH367 pKa = 6.13ATKK370 pKa = 10.6EE371 pKa = 4.35GAQKK375 pKa = 10.36SASIFEE381 pKa = 4.09NAINSLL387 pKa = 3.73

Molecular weight: 44.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

16

0

16

2389

41

387

149.3

17.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.735 ± 0.417

0.67 ± 0.2

5.818 ± 0.499

6.99 ± 0.79

3.6 ± 0.262

4.646 ± 0.355

1.088 ± 0.322

7.242 ± 0.612

10.423 ± 0.439

10.088 ± 0.571

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.842 ± 0.283

6.362 ± 0.504

2.679 ± 0.294

4.772 ± 0.511

4.353 ± 0.206

6.195 ± 0.55

6.697 ± 0.44

5.735 ± 0.375

0.921 ± 0.142

4.144 ± 0.435
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski