Capybara microvirus Cap1_SP_22
Average proteome isoelectric point is 6.04
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W4U5|A0A4P8W4U5_9VIRU Minor capsid protein OS=Capybara microvirus Cap1_SP_22 OX=2584777 PE=4 SV=1
MM1 pKa = 7.43 TNKK4 pKa = 9.88 QKK6 pKa = 10.95 FNGVNSFVVKK16 pKa = 10.87 LSDD19 pKa = 3.59 VPPFSSPTMLYY30 pKa = 10.79 CLDD33 pKa = 4.87 DD34 pKa = 4.55 DD35 pKa = 4.2 NKK37 pKa = 10.24 VRR39 pKa = 11.84 LKK41 pKa = 11.12 VVDD44 pKa = 3.18 KK45 pKa = 10.57 CLNLGPVSNYY55 pKa = 10.63 DD56 pKa = 3.65 LQTLIDD62 pKa = 3.75 NGVNISSMHH71 pKa = 5.99 YY72 pKa = 9.99 EE73 pKa = 4.15 PNDD76 pKa = 3.39 DD77 pKa = 3.62 RR78 pKa = 11.84 LLGVEE83 pKa = 4.63 RR84 pKa = 11.84 LNTFNPPADD93 pKa = 3.69 NDD95 pKa = 4.43 SNPNN99 pKa = 3.34
Molecular weight: 11.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.594
IPC2_protein 4.622
IPC_protein 4.495
Toseland 4.279
ProMoST 4.673
Dawson 4.495
Bjellqvist 4.647
Wikipedia 4.469
Rodwell 4.317
Grimsley 4.19
Solomon 4.495
Lehninger 4.457
Nozaki 4.622
DTASelect 4.914
Thurlkill 4.342
EMBOSS 4.482
Sillero 4.622
Patrickios 3.948
IPC_peptide 4.482
IPC2_peptide 4.596
IPC2.peptide.svr19 4.592
Protein with the highest isoelectric point:
>tr|A0A4P8W7F3|A0A4P8W7F3_9VIRU Peptidase M15 OS=Capybara microvirus Cap1_SP_22 OX=2584777 PE=4 SV=1
MM1 pKa = 7.31 LVSNPTNYY9 pKa = 7.45 VTPWQPVMLDD19 pKa = 3.48 VPNVRR24 pKa = 11.84 SFTYY28 pKa = 10.43 LSSLQRR34 pKa = 11.84 SYY36 pKa = 11.5 YY37 pKa = 8.68 IRR39 pKa = 11.84 SYY41 pKa = 11.77 YY42 pKa = 10.52 EE43 pKa = 3.61 FLEE46 pKa = 4.4 TKK48 pKa = 9.85 NRR50 pKa = 11.84 NGKK53 pKa = 8.17 TIFLTFNYY61 pKa = 9.53 NDD63 pKa = 3.34 NAIPSIYY70 pKa = 10.44 GIPCCNNDD78 pKa = 3.97 HH79 pKa = 6.38 IHH81 pKa = 5.82 YY82 pKa = 10.24 LLRR85 pKa = 11.84 NSSFIKK91 pKa = 9.73 WVRR94 pKa = 11.84 QHH96 pKa = 6.83 FDD98 pKa = 2.8 FSYY101 pKa = 10.4 IVCTEE106 pKa = 3.94 FGEE109 pKa = 4.85 GKK111 pKa = 10.31 GKK113 pKa = 10.23 RR114 pKa = 11.84 GKK116 pKa = 10.62 GNNPHH121 pKa = 5.37 YY122 pKa = 10.45 HH123 pKa = 6.26 ALFYY127 pKa = 10.95 LCHH130 pKa = 6.98 KK131 pKa = 10.26 DD132 pKa = 3.48 NSQVTPLDD140 pKa = 3.67 CAEE143 pKa = 3.95 VLSYY147 pKa = 10.23 IRR149 pKa = 11.84 KK150 pKa = 8.19 IWQGNPYY157 pKa = 9.38 KK158 pKa = 10.6 ASSKK162 pKa = 10.21 NDD164 pKa = 2.94 PRR166 pKa = 11.84 KK167 pKa = 9.78 FKK169 pKa = 10.49 FGKK172 pKa = 10.19 VEE174 pKa = 3.94 EE175 pKa = 4.52 SEE177 pKa = 4.22 KK178 pKa = 11.14 GLIVNDD184 pKa = 3.83 FHH186 pKa = 7.36 AANYY190 pKa = 7.82 VSKK193 pKa = 10.94 YY194 pKa = 7.19 ATKK197 pKa = 10.6 DD198 pKa = 3.25 KK199 pKa = 8.97 EE200 pKa = 4.66 TKK202 pKa = 10.42 CIIRR206 pKa = 11.84 KK207 pKa = 8.87 ILNAHH212 pKa = 6.32 YY213 pKa = 10.21 NDD215 pKa = 4.41 CLHH218 pKa = 6.75 KK219 pKa = 10.94 VEE221 pKa = 4.96 LDD223 pKa = 3.18 EE224 pKa = 5.7 SINHH228 pKa = 5.79 TRR230 pKa = 11.84 LSLVDD235 pKa = 3.8 PFVMPGTTFNDD246 pKa = 3.55 KK247 pKa = 10.54 VDD249 pKa = 3.36 IMVKK253 pKa = 9.85 QDD255 pKa = 3.17 AQILYY260 pKa = 10.53 DD261 pKa = 3.77 EE262 pKa = 4.71 TLPTIKK268 pKa = 9.52 KK269 pKa = 7.71 TFLPKK274 pKa = 10.53 VFMSKK279 pKa = 10.41 GFGSSASKK287 pKa = 11.03 YY288 pKa = 8.71 ITDD291 pKa = 4.06 DD292 pKa = 3.24 FKK294 pKa = 11.46 MPILTHH300 pKa = 6.61 KK301 pKa = 8.96 GTIYY305 pKa = 9.7 VTLPYY310 pKa = 10.17 YY311 pKa = 10.21 IYY313 pKa = 10.73 RR314 pKa = 11.84 KK315 pKa = 7.8 TFYY318 pKa = 10.42 DD319 pKa = 3.38 VKK321 pKa = 10.96 KK322 pKa = 9.92 NANGNVIYY330 pKa = 10.6 VLNNVGTEE338 pKa = 3.85 YY339 pKa = 10.79 RR340 pKa = 11.84 KK341 pKa = 10.04 NRR343 pKa = 11.84 LSKK346 pKa = 10.45 DD347 pKa = 2.96 IRR349 pKa = 11.84 DD350 pKa = 4.29 LYY352 pKa = 10.68 SKK354 pKa = 10.49 CISILHH360 pKa = 6.24 NSDD363 pKa = 3.2 TLSNNDD369 pKa = 3.58 SEE371 pKa = 4.38 EE372 pKa = 4.46 DD373 pKa = 3.13 IYY375 pKa = 11.76 ANKK378 pKa = 9.36 IRR380 pKa = 11.84 HH381 pKa = 5.53 LHH383 pKa = 6.26 HH384 pKa = 6.51 SLKK387 pKa = 10.5 SHH389 pKa = 6.07 VSAPFLDD396 pKa = 4.25 PMHH399 pKa = 7.22 AYY401 pKa = 10.26 ASYY404 pKa = 10.66 KK405 pKa = 10.26 YY406 pKa = 9.45 IYY408 pKa = 9.78 QYY410 pKa = 11.27 RR411 pKa = 11.84 CFEE414 pKa = 4.1 GLEE417 pKa = 4.23 FPLDD421 pKa = 4.03 LNDD424 pKa = 5.31 DD425 pKa = 4.09 FALKK429 pKa = 10.43 LLPGYY434 pKa = 10.73 YY435 pKa = 10.0 YY436 pKa = 11.35 GEE438 pKa = 4.37 FSNSPILTYY447 pKa = 10.65 KK448 pKa = 10.61 EE449 pKa = 3.89 LTKK452 pKa = 11.07 SNWHH456 pKa = 6.72 PFSEE460 pKa = 4.47 HH461 pKa = 6.37 SSFVPLIPLFNVLDD475 pKa = 3.96 KK476 pKa = 11.64 YY477 pKa = 11.24 FDD479 pKa = 3.74 LVGIQLDD486 pKa = 3.44 DD487 pKa = 3.63 ARR489 pKa = 11.84 YY490 pKa = 9.85 KK491 pKa = 10.51 RR492 pKa = 11.84 YY493 pKa = 9.57 LRR495 pKa = 11.84 KK496 pKa = 10.11 KK497 pKa = 8.22 MLFNFFSKK505 pKa = 10.69 YY506 pKa = 8.9 KK507 pKa = 10.44 RR508 pKa = 11.84 KK509 pKa = 10.1
Molecular weight: 59.78 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.548
IPC2_protein 8.551
IPC_protein 8.404
Toseland 8.873
ProMoST 8.902
Dawson 9.268
Bjellqvist 9.136
Wikipedia 9.458
Rodwell 9.399
Grimsley 9.326
Solomon 9.297
Lehninger 9.253
Nozaki 9.151
DTASelect 9.048
Thurlkill 9.121
EMBOSS 9.37
Sillero 9.282
Patrickios 3.986
IPC_peptide 9.282
IPC2_peptide 7.834
IPC2.peptide.svr19 7.723
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
1713
99
583
342.6
39.12
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.546 ± 1.268
1.693 ± 0.428
6.421 ± 0.612
4.32 ± 0.789
5.838 ± 0.889
5.137 ± 0.605
2.393 ± 0.568
5.721 ± 0.397
6.83 ± 0.874
9.048 ± 0.302
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.926 ± 0.353
7.823 ± 0.883
3.853 ± 0.614
3.736 ± 1.085
3.678 ± 0.256
8.173 ± 0.624
6.305 ± 0.698
4.845 ± 0.737
0.992 ± 0.304
5.721 ± 1.095
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here