Cyclobacterium marinum (strain ATCC 25205 / DSM 745 / LMG 13164 / NCIMB 1802) (Flectobacillus marinus)

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Cytophagia; Cytophagales; Cyclobacteriaceae; Cyclobacterium; Cyclobacterium marinum

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4983 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G0J4Y2|G0J4Y2_CYCMS Uncharacterized protein OS=Cyclobacterium marinum (strain ATCC 25205 / DSM 745 / LMG 13164 / NCIMB 1802) OX=880070 GN=Cycma_2234 PE=4 SV=1
MM1 pKa = 7.54KK2 pKa = 10.31SFHH5 pKa = 6.71KK6 pKa = 10.19FVLVIVSISFFQTGCNTGMDD26 pKa = 3.95EE27 pKa = 5.43DD28 pKa = 4.33IPSISDD34 pKa = 3.47YY35 pKa = 11.34SGTASISQGFATATINNLFEE55 pKa = 4.54CSNGRR60 pKa = 11.84DD61 pKa = 3.59APLGISTAMDD71 pKa = 4.22GSTWTVPAQVNFNDD85 pKa = 3.85SNFPFASDD93 pKa = 4.68LYY95 pKa = 9.79NPCSGLTFNTDD106 pKa = 3.07TEE108 pKa = 4.35AVLALKK114 pKa = 10.67DD115 pKa = 3.59EE116 pKa = 5.53DD117 pKa = 4.45IIEE120 pKa = 4.14VDD122 pKa = 3.76PNGEE126 pKa = 4.51LITAFVFADD135 pKa = 4.12NYY137 pKa = 10.72FEE139 pKa = 4.36MYY141 pKa = 10.37INGVPVGKK149 pKa = 10.35DD150 pKa = 3.04MVPFTPFNSSILRR163 pKa = 11.84FRR165 pKa = 11.84VSYY168 pKa = 10.38PFTIAMKK175 pKa = 10.59LVDD178 pKa = 3.52WEE180 pKa = 4.48EE181 pKa = 3.85NSGLGSEE188 pKa = 4.62SNQGASFYY196 pKa = 10.38PGDD199 pKa = 3.78GGMVAVFKK207 pKa = 10.9NQANEE212 pKa = 3.83IVATTGADD220 pKa = 2.85WKK222 pKa = 10.91AQTFYY227 pKa = 10.9TSPIKK232 pKa = 10.51DD233 pKa = 3.9LSCTTEE239 pKa = 3.61NGTQRR244 pKa = 11.84LSYY247 pKa = 10.73NCNTDD252 pKa = 2.77GANDD256 pKa = 3.87GTSFYY261 pKa = 10.92ALHH264 pKa = 6.85WDD266 pKa = 4.46IPANWQEE273 pKa = 3.81EE274 pKa = 4.28DD275 pKa = 4.17FDD277 pKa = 5.16DD278 pKa = 5.46SSWPNATPYY287 pKa = 10.38TNTEE291 pKa = 3.36IGVNNKK297 pKa = 8.81PAYY300 pKa = 9.55TNFTSIFDD308 pKa = 4.18DD309 pKa = 5.32PIDD312 pKa = 4.0DD313 pKa = 5.89AEE315 pKa = 5.13FIWSTNVILDD325 pKa = 3.38NEE327 pKa = 4.63VIVRR331 pKa = 11.84FTVEE335 pKa = 3.35

Molecular weight: 36.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G0IYF5|G0IYF5_CYCMS Transcription termination/antitermination protein NusA OS=Cyclobacterium marinum (strain ATCC 25205 / DSM 745 / LMG 13164 / NCIMB 1802) OX=880070 GN=nusA PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.56RR3 pKa = 11.84TFQPSRR9 pKa = 11.84RR10 pKa = 11.84KK11 pKa = 9.62RR12 pKa = 11.84RR13 pKa = 11.84NKK15 pKa = 9.5HH16 pKa = 3.99GFRR19 pKa = 11.84EE20 pKa = 4.15RR21 pKa = 11.84MSTSNGRR28 pKa = 11.84RR29 pKa = 11.84VLKK32 pKa = 10.5ARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.17GRR39 pKa = 11.84HH40 pKa = 5.24KK41 pKa = 10.87LSVSSEE47 pKa = 3.97KK48 pKa = 9.91TLKK51 pKa = 10.51KK52 pKa = 10.72

Molecular weight: 6.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4983

0

4983

1776705

30

3154

356.6

40.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.469 ± 0.034

0.695 ± 0.011

5.443 ± 0.022

6.869 ± 0.027

5.137 ± 0.024

7.036 ± 0.034

1.846 ± 0.015

7.397 ± 0.031

7.117 ± 0.038

9.732 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.417 ± 0.018

5.67 ± 0.035

3.945 ± 0.021

3.504 ± 0.018

3.763 ± 0.02

6.594 ± 0.025

5.075 ± 0.021

6.053 ± 0.024

1.303 ± 0.015

3.935 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski