Clostridium baratii str. Sullivan

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; Clostridium baratii

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3167 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A7G2V8|A0A0A7G2V8_9CLOT Uncharacterized protein OS=Clostridium baratii str. Sullivan OX=1415775 GN=U729_3215 PE=4 SV=1
MM1 pKa = 7.88DD2 pKa = 5.25RR3 pKa = 11.84KK4 pKa = 8.39TVLLMACRR12 pKa = 11.84DD13 pKa = 3.77LLKK16 pKa = 10.78KK17 pKa = 10.33QINSIYY23 pKa = 10.38ILDD26 pKa = 4.39LLSEE30 pKa = 4.34TVFYY34 pKa = 11.14DD35 pKa = 4.12GADD38 pKa = 3.43CDD40 pKa = 5.06GYY42 pKa = 11.54CLLEE46 pKa = 5.1DD47 pKa = 4.54IEE49 pKa = 5.93AEE51 pKa = 4.74LDD53 pKa = 3.51IQEE56 pKa = 4.54DD57 pKa = 3.67

Molecular weight: 6.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A7FV62|A0A0A7FV62_9CLOT Putative ribosomal protein L14E OS=Clostridium baratii str. Sullivan OX=1415775 GN=U729_1369 PE=4 SV=1
MM1 pKa = 7.49EE2 pKa = 4.96EE3 pKa = 4.11KK4 pKa = 10.78NYY6 pKa = 11.07GLTNDD11 pKa = 3.81GLRR14 pKa = 11.84NILNYY19 pKa = 8.24TIRR22 pKa = 11.84VIVFVCGIFILALGAATLITANVGVATWDD51 pKa = 3.41VLHH54 pKa = 7.02IGLARR59 pKa = 11.84ISSLSVGRR67 pKa = 11.84WVQIVGITMVLMTCVFEE84 pKa = 4.57RR85 pKa = 11.84KK86 pKa = 9.38RR87 pKa = 11.84PVLGSVLNIVLVGFFLNFILSVNIIPHH114 pKa = 6.4FDD116 pKa = 3.66NIGARR121 pKa = 11.84ILLLIIGIILMGIGSGMYY139 pKa = 9.1VASKK143 pKa = 10.59VGAGPRR149 pKa = 11.84DD150 pKa = 3.52GMTLFLARR158 pKa = 11.84RR159 pKa = 11.84FSISVRR165 pKa = 11.84LARR168 pKa = 11.84TILEE172 pKa = 4.0ISALTIGWLIGGPVSIGTFISVPLIGPVMQASLKK206 pKa = 9.8VWTIILKK213 pKa = 10.34KK214 pKa = 10.53LYY216 pKa = 10.5

Molecular weight: 23.42 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3167

0

3167

965364

29

2939

304.8

34.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.318 ± 0.05

1.14 ± 0.017

5.796 ± 0.038

7.686 ± 0.063

4.481 ± 0.032

6.488 ± 0.046

1.251 ± 0.017

10.073 ± 0.054

9.509 ± 0.052

8.985 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.537 ± 0.022

6.672 ± 0.05

2.657 ± 0.027

1.899 ± 0.019

3.341 ± 0.028

6.134 ± 0.031

4.82 ± 0.036

6.43 ± 0.039

0.636 ± 0.016

4.148 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski