Wenzhou shrimp virus 3

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 5.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KK05|A0A1L3KK05_9VIRU Replicase OS=Wenzhou shrimp virus 3 OX=1923650 PE=4 SV=1
MM1 pKa = 6.31EE2 pKa = 4.53QYY4 pKa = 10.67RR5 pKa = 11.84IFDD8 pKa = 4.56DD9 pKa = 5.32DD10 pKa = 3.76FHH12 pKa = 7.9PCISPQRR19 pKa = 11.84FPDD22 pKa = 3.49ALLRR26 pKa = 11.84IYY28 pKa = 11.2AHH30 pKa = 6.59FDD32 pKa = 3.63GIIEE36 pKa = 4.29HH37 pKa = 6.94PVLEE41 pKa = 4.2QEE43 pKa = 4.24LDD45 pKa = 3.54FRR47 pKa = 11.84VAGDD51 pKa = 3.56PPVIRR56 pKa = 11.84VTTSDD61 pKa = 3.56FNGRR65 pKa = 11.84PHH67 pKa = 6.5LTSSIFVEE75 pKa = 4.01WTYY78 pKa = 11.98DD79 pKa = 3.65EE80 pKa = 4.42ILHH83 pKa = 6.61MIRR86 pKa = 11.84MHH88 pKa = 6.71YY89 pKa = 10.08EE90 pKa = 3.88FGYY93 pKa = 10.84DD94 pKa = 2.84IDD96 pKa = 4.57LNAMVTLGFDD106 pKa = 3.73PMDD109 pKa = 3.1VWEE112 pKa = 4.54LTNAEE117 pKa = 4.57RR118 pKa = 11.84FNIDD122 pKa = 3.28VPDD125 pKa = 3.71MHH127 pKa = 7.36EE128 pKa = 5.04DD129 pKa = 3.4APPPNGEE136 pKa = 4.16YY137 pKa = 10.91YY138 pKa = 10.91YY139 pKa = 11.02DD140 pKa = 4.62ADD142 pKa = 4.17DD143 pKa = 4.85EE144 pKa = 4.38EE145 pKa = 5.1MEE147 pKa = 4.7VDD149 pKa = 4.19DD150 pKa = 6.86DD151 pKa = 5.43YY152 pKa = 11.39ITEE155 pKa = 4.45SEE157 pKa = 4.23DD158 pKa = 3.24EE159 pKa = 5.16GYY161 pKa = 11.03DD162 pKa = 3.95SYY164 pKa = 12.16DD165 pKa = 3.29PYY167 pKa = 11.32FNSSDD172 pKa = 3.66EE173 pKa = 4.65EE174 pKa = 4.58YY175 pKa = 10.79DD176 pKa = 3.75GPLPNILHH184 pKa = 6.99DD185 pKa = 3.89

Molecular weight: 21.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KJY5|A0A1L3KJY5_9VIRU Uncharacterized protein OS=Wenzhou shrimp virus 3 OX=1923650 PE=4 SV=1
MM1 pKa = 7.33AAKK4 pKa = 9.21TRR6 pKa = 11.84LIEE9 pKa = 3.82VLRR12 pKa = 11.84AQGVTPTYY20 pKa = 10.22SCKK23 pKa = 10.22LALTTRR29 pKa = 11.84VGDD32 pKa = 3.73EE33 pKa = 3.83EE34 pKa = 5.09VPFVNLPVFVCDD46 pKa = 4.29LYY48 pKa = 11.38FVVKK52 pKa = 9.79TPFGDD57 pKa = 3.8QIHH60 pKa = 6.9EE61 pKa = 4.06YY62 pKa = 9.76SARR65 pKa = 11.84ATTKK69 pKa = 10.38LAAQNICSALALEE82 pKa = 4.83KK83 pKa = 10.76VDD85 pKa = 4.38DD86 pKa = 3.97VTRR89 pKa = 11.84EE90 pKa = 3.89RR91 pKa = 11.84KK92 pKa = 8.72PVYY95 pKa = 9.55PFQVVNMQGIRR106 pKa = 11.84YY107 pKa = 6.12YY108 pKa = 10.63TPAEE112 pKa = 3.95LLRR115 pKa = 11.84FAVEE119 pKa = 4.29NSLTTYY125 pKa = 10.89GFF127 pKa = 3.66

Molecular weight: 14.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

3177

127

2229

635.4

71.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.341 ± 0.802

1.794 ± 0.379

6.641 ± 1.056

6.956 ± 0.563

4.249 ± 0.41

5.666 ± 0.574

2.801 ± 0.487

5.54 ± 0.351

5.886 ± 1.301

7.428 ± 0.474

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.361 ± 0.194

4.47 ± 0.226

5.319 ± 0.581

3.714 ± 0.538

5.036 ± 0.274

6.075 ± 1.283

5.823 ± 1.197

7.177 ± 0.476

1.102 ± 0.292

3.62 ± 0.695
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski