Maize yellow mosaic virus
Average proteome isoelectric point is 8.23
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A191T7R1|A0A191T7R1_9LUTE Coat protein OS=Maize yellow mosaic virus OX=1856642 PE=4 SV=1
MM1 pKa = 7.22 QKK3 pKa = 10.27 LSSVAFFLCCLLCYY17 pKa = 9.06 ATYY20 pKa = 7.68 PTRR23 pKa = 11.84 GDD25 pKa = 3.53 FLEE28 pKa = 4.6 MYY30 pKa = 9.86 GLPLRR35 pKa = 11.84 GTQFGLEE42 pKa = 4.18 EE43 pKa = 4.16 TSLLPRR49 pKa = 11.84 NASLPFKK56 pKa = 10.59 FLLAPDD62 pKa = 3.62 SCIEE66 pKa = 3.73 ISYY69 pKa = 10.17 SALSRR74 pKa = 11.84 EE75 pKa = 4.26 IARR78 pKa = 11.84 LALQDD83 pKa = 3.56 LHH85 pKa = 6.63 TISLAGSRR93 pKa = 11.84 ALSSSWKK100 pKa = 10.38 GFAEE104 pKa = 4.14 EE105 pKa = 4.53 WNTSVVKK112 pKa = 10.4 LAHH115 pKa = 5.96 ATSGFYY121 pKa = 9.9 QQASDD126 pKa = 4.03 YY127 pKa = 10.88 VVSCVLEE134 pKa = 4.0 LLGSCLVAIIYY145 pKa = 7.61 TWSFFISSLFWVVTFMLQHH164 pKa = 5.16 YY165 pKa = 6.74 TPHH168 pKa = 5.91 VLSIAVLYY176 pKa = 10.43 ICTTLLVRR184 pKa = 11.84 LATKK188 pKa = 10.33 IFGGWPVMMISWIGTSTLGFSKK210 pKa = 9.68 TLLFKK215 pKa = 10.9 GSSISYY221 pKa = 7.6 EE222 pKa = 3.92 IPTPGYY228 pKa = 10.11 KK229 pKa = 10.02 SIEE232 pKa = 4.21 IPQKK236 pKa = 9.98 PPKK239 pKa = 10.48 DD240 pKa = 3.44 CVLLVQHH247 pKa = 6.82 NDD249 pKa = 3.16 ALNSPGGYY257 pKa = 9.68 ASCVRR262 pKa = 11.84 LLNGSNALLTAEE274 pKa = 4.67 HH275 pKa = 6.17 VAAQKK280 pKa = 11.34 GEE282 pKa = 4.17 LLVASTRR289 pKa = 11.84 TGNKK293 pKa = 9.14 IKK295 pKa = 10.79 LSLFNRR301 pKa = 11.84 ILTTTNSDD309 pKa = 2.75 VSLFQGPPGWEE320 pKa = 3.79 SLLGCKK326 pKa = 9.73 AADD329 pKa = 3.37 ITPVDD334 pKa = 3.66 GLTTCEE340 pKa = 3.58 ASLFRR345 pKa = 11.84 FDD347 pKa = 3.99 GHH349 pKa = 5.47 WMRR352 pKa = 11.84 SNASLVGTEE361 pKa = 3.87 EE362 pKa = 4.7 TYY364 pKa = 11.51 VSVLSNTEE372 pKa = 3.66 EE373 pKa = 5.09 GYY375 pKa = 10.92 SGTPYY380 pKa = 10.68 FNGKK384 pKa = 9.77 SILGVHH390 pKa = 5.99 VGGNATQTNNLMAPIPSIPGLTKK413 pKa = 9.72 HH414 pKa = 6.43 RR415 pKa = 11.84 YY416 pKa = 8.53 VFEE419 pKa = 4.19 SPQLKK424 pKa = 10.0 GRR426 pKa = 11.84 LFTEE430 pKa = 4.21 QEE432 pKa = 4.17 VEE434 pKa = 4.07 QLEE437 pKa = 4.18 VDD439 pKa = 2.85 IDD441 pKa = 3.86 EE442 pKa = 4.97 AFKK445 pKa = 10.67 KK446 pKa = 10.64 AYY448 pKa = 10.25 DD449 pKa = 3.72 LVHH452 pKa = 6.8 FKK454 pKa = 11.15 SKK456 pKa = 9.38 TGRR459 pKa = 11.84 NWADD463 pKa = 3.29 YY464 pKa = 10.39 EE465 pKa = 4.56 DD466 pKa = 5.73 DD467 pKa = 3.87 ISYY470 pKa = 8.15 EE471 pKa = 3.93 APKK474 pKa = 10.42 FQGKK478 pKa = 9.11 RR479 pKa = 11.84 RR480 pKa = 11.84 TRR482 pKa = 11.84 FRR484 pKa = 11.84 PRR486 pKa = 11.84 KK487 pKa = 9.06 QNGLAHH493 pKa = 7.32 PYY495 pKa = 10.16 SLARR499 pKa = 11.84 RR500 pKa = 11.84 YY501 pKa = 8.86 FKK503 pKa = 10.54 QRR505 pKa = 11.84 YY506 pKa = 6.24 SQQGGGSVGQQDD518 pKa = 3.19 RR519 pKa = 11.84 RR520 pKa = 11.84 LSHH523 pKa = 6.74 RR524 pKa = 11.84 ADD526 pKa = 3.47 GCSTNCGISHH536 pKa = 6.56 EE537 pKa = 4.61 TPKK540 pKa = 10.26 EE541 pKa = 3.89 QPEE544 pKa = 4.09 AAIKK548 pKa = 9.63 AQNFASYY555 pKa = 10.39 FSSLYY560 pKa = 9.86 KK561 pKa = 10.17 WEE563 pKa = 4.26 VCDD566 pKa = 4.51 TRR568 pKa = 11.84 SEE570 pKa = 4.45 TPGFEE575 pKa = 4.49 TCGSLPRR582 pKa = 11.84 YY583 pKa = 9.85 YY584 pKa = 9.53 FTKK587 pKa = 10.4 QKK589 pKa = 10.9 EE590 pKa = 3.93 EE591 pKa = 4.42 SEE593 pKa = 4.13 WGRR596 pKa = 11.84 LLAEE600 pKa = 4.72 GNPALAEE607 pKa = 4.16 KK608 pKa = 10.7 VSGFGWPQFGPAAEE622 pKa = 4.29 LKK624 pKa = 10.42 SLRR627 pKa = 11.84 LQAQRR632 pKa = 11.84 WLSRR636 pKa = 11.84 AEE638 pKa = 3.99 SAKK641 pKa = 10.38 IPSAEE646 pKa = 3.78 DD647 pKa = 3.1 RR648 pKa = 11.84 EE649 pKa = 4.24 RR650 pKa = 11.84 VIRR653 pKa = 11.84 KK654 pKa = 6.66 TVEE657 pKa = 3.73 AYY659 pKa = 7.7 KK660 pKa = 9.8 TCQTQCPKK668 pKa = 10.3 TSQSNLLVWEE678 pKa = 4.38 NFLEE682 pKa = 4.3 DD683 pKa = 4.04 FKK685 pKa = 11.47 QAVFSLEE692 pKa = 3.57 PDD694 pKa = 3.26 AGVGVPFVGYY704 pKa = 10.19 DD705 pKa = 3.04 KK706 pKa = 9.97 RR707 pKa = 11.84 THH709 pKa = 6.47 RR710 pKa = 11.84 GWIEE714 pKa = 4.45 DD715 pKa = 3.71 PTLLPVLARR724 pKa = 11.84 MTFDD728 pKa = 4.25 RR729 pKa = 11.84 LHH731 pKa = 6.74 KK732 pKa = 9.41 MSTVKK737 pKa = 10.56 FEE739 pKa = 4.37 HH740 pKa = 6.11 LTAEE744 pKa = 4.34 EE745 pKa = 4.14 LVQAGLCDD753 pKa = 5.02 PIRR756 pKa = 11.84 LFVKK760 pKa = 10.72 GEE762 pKa = 3.72 PHH764 pKa = 6.07 KK765 pKa = 10.35 QAKK768 pKa = 9.74 LDD770 pKa = 3.49 EE771 pKa = 4.35 GRR773 pKa = 11.84 YY774 pKa = 8.74 RR775 pKa = 11.84 LIMSVSLLDD784 pKa = 3.51 QLVARR789 pKa = 11.84 VLFQNQNKK797 pKa = 9.66 RR798 pKa = 11.84 EE799 pKa = 3.93 IALWRR804 pKa = 11.84 AIPSKK809 pKa = 10.55 PGFGLSTDD817 pKa = 3.55 DD818 pKa = 3.46 QSRR821 pKa = 11.84 EE822 pKa = 3.91 FVQNLARR829 pKa = 11.84 QCGVSSDD836 pKa = 3.95 RR837 pKa = 11.84 LLQEE841 pKa = 4.21 WQLHH845 pKa = 4.78 TVPTDD850 pKa = 3.2 CSGFDD855 pKa = 3.21 WSVAEE860 pKa = 5.47 WMLQDD865 pKa = 4.67 DD866 pKa = 4.11 MEE868 pKa = 4.39 VRR870 pKa = 11.84 NRR872 pKa = 11.84 LTRR875 pKa = 11.84 NNTDD879 pKa = 2.87 LTRR882 pKa = 11.84 RR883 pKa = 11.84 LRR885 pKa = 11.84 ACWLKK890 pKa = 10.91 CISNSVLCLSDD901 pKa = 3.31 GTLLAQRR908 pKa = 11.84 VAGVQKK914 pKa = 10.39 SGSYY918 pKa = 8.25 NTSSSNSRR926 pKa = 11.84 IRR928 pKa = 11.84 VMAAYY933 pKa = 9.93 HH934 pKa = 6.74 CGATWAMAMGDD945 pKa = 3.95 DD946 pKa = 4.15 ALEE949 pKa = 4.37 SVDD952 pKa = 3.62 TCLDD956 pKa = 3.43 VYY958 pKa = 11.17 KK959 pKa = 10.94 DD960 pKa = 3.43 LGFKK964 pKa = 10.81 VEE966 pKa = 4.03 VSKK969 pKa = 10.95 QLEE972 pKa = 4.41 FCSHH976 pKa = 4.85 VFEE979 pKa = 5.34 KK980 pKa = 10.75 EE981 pKa = 3.64 HH982 pKa = 6.75 LARR985 pKa = 11.84 PVNQNKK991 pKa = 9.32 MIYY994 pKa = 9.64 KK995 pKa = 10.2 LVYY998 pKa = 9.95 GYY1000 pKa = 11.16 NPANGSSEE1008 pKa = 4.05 VLQRR1012 pKa = 11.84 YY1013 pKa = 9.24 LDD1015 pKa = 3.37 ACMSVLHH1022 pKa = 6.23 EE1023 pKa = 4.64 LRR1025 pKa = 11.84 HH1026 pKa = 6.05 DD1027 pKa = 4.05 PEE1029 pKa = 4.66 TVEE1032 pKa = 6.24 LLYY1035 pKa = 10.63 KK1036 pKa = 10.04 WLVSPVQQQKK1046 pKa = 8.01 VV1047 pKa = 3.19
Molecular weight: 117.67 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.926
IPC2_protein 7.249
IPC_protein 7.059
Toseland 6.781
ProMoST 7.673
Dawson 7.746
Bjellqvist 8.2
Wikipedia 7.658
Rodwell 7.775
Grimsley 6.854
Solomon 7.761
Lehninger 7.79
Nozaki 8.448
DTASelect 7.922
Thurlkill 7.966
EMBOSS 7.98
Sillero 8.302
Patrickios 4.151
IPC_peptide 7.761
IPC2_peptide 7.366
IPC2.peptide.svr19 7.244
Protein with the highest isoelectric point:
>tr|A0A191T7S4|A0A191T7S4_9LUTE Movement protein OS=Maize yellow mosaic virus OX=1856642 PE=3 SV=1
MM1 pKa = 6.87 NTGGRR6 pKa = 11.84 NGRR9 pKa = 11.84 RR10 pKa = 11.84 ARR12 pKa = 11.84 NRR14 pKa = 11.84 RR15 pKa = 11.84 RR16 pKa = 11.84 ARR18 pKa = 11.84 NNNRR22 pKa = 11.84 AQPVVVVAANPRR34 pKa = 11.84 RR35 pKa = 11.84 GRR37 pKa = 11.84 PRR39 pKa = 11.84 RR40 pKa = 11.84 RR41 pKa = 11.84 RR42 pKa = 11.84 RR43 pKa = 11.84 PSGNTAGRR51 pKa = 11.84 PGVRR55 pKa = 11.84 RR56 pKa = 11.84 GSRR59 pKa = 11.84 EE60 pKa = 3.68 TFVFSKK66 pKa = 11.02 DD67 pKa = 3.43 SLTGNASGKK76 pKa = 7.79 VTFGPSLSEE85 pKa = 3.84 CAAFSGGILKK95 pKa = 10.27 AYY97 pKa = 10.21 HH98 pKa = 6.75 EE99 pKa = 4.41 YY100 pKa = 10.44 KK101 pKa = 9.82 ISKK104 pKa = 9.83 IILEE108 pKa = 5.09 FISEE112 pKa = 4.21 AASTAEE118 pKa = 3.73 GSIAYY123 pKa = 9.5 EE124 pKa = 3.8 LDD126 pKa = 3.19 PHH128 pKa = 6.74 NKK130 pKa = 9.68 LSSLASTINKK140 pKa = 9.5 FSIVKK145 pKa = 9.57 GGRR148 pKa = 11.84 RR149 pKa = 11.84 VFASNQIGGGVWRR162 pKa = 11.84 DD163 pKa = 3.37 SSEE166 pKa = 4.04 DD167 pKa = 3.28 QCAILYY173 pKa = 9.54 KK174 pKa = 10.95 GNGKK178 pKa = 10.21 SSVAGSFRR186 pKa = 11.84 ITIEE190 pKa = 4.21 VNVQNPKK197 pKa = 10.48
Molecular weight: 21.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.193
IPC2_protein 10.072
IPC_protein 11.345
Toseland 11.491
ProMoST 11.915
Dawson 11.506
Bjellqvist 11.447
Wikipedia 11.93
Rodwell 11.418
Grimsley 11.55
Solomon 11.945
Lehninger 11.857
Nozaki 11.491
DTASelect 11.447
Thurlkill 11.491
EMBOSS 11.974
Sillero 11.491
Patrickios 11.125
IPC_peptide 11.945
IPC2_peptide 10.906
IPC2.peptide.svr19 9.243
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
2986
192
1047
497.7
55.21
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.368 ± 0.48
1.808 ± 0.367
4.086 ± 0.364
5.626 ± 0.3
4.052 ± 0.491
7.167 ± 0.455
1.842 ± 0.377
4.153 ± 0.329
4.789 ± 0.471
9.277 ± 1.564
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.44 ± 0.152
4.253 ± 0.67
5.894 ± 0.847
4.22 ± 0.356
6.899 ± 1.043
9.913 ± 0.699
6.095 ± 0.363
6.196 ± 0.323
1.574 ± 0.236
3.315 ± 0.241
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here