Sulfolobales archaeon SCGC AB-777_J03

Taxonomy: cellular organisms; Archaea; TACK group; Crenarchaeota; Thermoprotei; Sulfolobales; unclassified Sulfolobales

Average proteome isoelectric point is 6.84

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 928 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2T9WYF3|A0A2T9WYF3_9CREN Site-specific DNA-methyltransferase (adenine-specific) OS=Sulfolobales archaeon SCGC AB-777_J03 OX=2005259 GN=DDW10_03550 PE=4 SV=1
MM1 pKa = 7.54QDD3 pKa = 3.87VIWDD7 pKa = 3.65GQYY10 pKa = 11.7ANTIIQNHH18 pKa = 4.75NQIVQLNGQFVGQTDD33 pKa = 3.69PVNNQPYY40 pKa = 9.83VPLQDD45 pKa = 4.18FYY47 pKa = 11.6VIKK50 pKa = 10.59GQVPIMNTTINGQTYY65 pKa = 9.23YY66 pKa = 11.12VIDD69 pKa = 4.29ANNINPADD77 pKa = 3.49VAGFYY82 pKa = 9.72TYY84 pKa = 10.53QGWVPNFVEE93 pKa = 5.01AMNTPGTYY101 pKa = 10.45AAVLPGNSPVFQWTNTTGTVAYY123 pKa = 7.58QTLL126 pKa = 3.45

Molecular weight: 14.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2T9WZ52|A0A2T9WZ52_9CREN 50S ribosomal protein L15 OS=Sulfolobales archaeon SCGC AB-777_J03 OX=2005259 GN=rpl15 PE=3 SV=1
MM1 pKa = 7.73PRR3 pKa = 11.84PALRR7 pKa = 11.84SRR9 pKa = 11.84SLARR13 pKa = 11.84YY14 pKa = 7.55YY15 pKa = 11.08VRR17 pKa = 11.84TPGGRR22 pKa = 11.84TVIHH26 pKa = 6.05YY27 pKa = 7.5EE28 pKa = 3.76RR29 pKa = 11.84KK30 pKa = 9.29RR31 pKa = 11.84NGKK34 pKa = 7.87ATCAVCGGEE43 pKa = 4.4LRR45 pKa = 11.84GVPTNQVYY53 pKa = 10.02KK54 pKa = 10.51YY55 pKa = 10.08SKK57 pKa = 8.34TEE59 pKa = 3.68KK60 pKa = 10.37RR61 pKa = 11.84PEE63 pKa = 3.68RR64 pKa = 11.84PYY66 pKa = 11.4AGTLCHH72 pKa = 6.79RR73 pKa = 11.84CLEE76 pKa = 4.16KK77 pKa = 10.69LIKK80 pKa = 9.84QAVRR84 pKa = 11.84SS85 pKa = 3.89

Molecular weight: 9.74 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

928

0

928

246088

22

1497

265.2

29.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.114 ± 0.083

0.671 ± 0.028

4.924 ± 0.057

7.971 ± 0.083

3.857 ± 0.054

7.001 ± 0.063

1.37 ± 0.029

6.455 ± 0.069

7.018 ± 0.085

10.627 ± 0.097

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.191 ± 0.039

3.5 ± 0.063

4.224 ± 0.053

2.109 ± 0.042

6.101 ± 0.079

6.216 ± 0.059

4.622 ± 0.061

9.13 ± 0.075

0.961 ± 0.026

3.939 ± 0.051
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski