Amino acid dipepetide frequency for Gordonia phage SketchMex

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.932AlaAla: 10.932 ± 1.66
0.487AlaCys: 0.487 ± 0.165
5.57AlaAsp: 5.57 ± 0.711
5.849AlaGlu: 5.849 ± 0.747
3.76AlaPhe: 3.76 ± 0.822
7.589AlaGly: 7.589 ± 0.931
1.81AlaHis: 1.81 ± 0.328
5.361AlaIle: 5.361 ± 0.638
4.387AlaLys: 4.387 ± 0.576
9.4AlaLeu: 9.4 ± 0.855
2.298AlaMet: 2.298 ± 0.422
2.855AlaAsn: 2.855 ± 0.576
4.247AlaPro: 4.247 ± 0.532
2.228AlaGln: 2.228 ± 0.359
5.779AlaArg: 5.779 ± 0.644
6.336AlaSer: 6.336 ± 0.877
6.267AlaThr: 6.267 ± 0.718
6.754AlaVal: 6.754 ± 1.18
2.715AlaTrp: 2.715 ± 0.453
2.855AlaTyr: 2.855 ± 0.374
0.0AlaXaa: 0.0 ± 0.0
Cys
0.766CysAla: 0.766 ± 0.281
0.07CysCys: 0.07 ± 0.059
0.627CysAsp: 0.627 ± 0.173
0.487CysGlu: 0.487 ± 0.183
0.348CysPhe: 0.348 ± 0.188
0.975CysGly: 0.975 ± 0.282
0.07CysHis: 0.07 ± 0.079
0.279CysIle: 0.279 ± 0.143
0.209CysLys: 0.209 ± 0.115
0.627CysLeu: 0.627 ± 0.253
0.139CysMet: 0.139 ± 0.096
0.139CysAsn: 0.139 ± 0.11
0.557CysPro: 0.557 ± 0.227
0.209CysGln: 0.209 ± 0.115
0.418CysArg: 0.418 ± 0.166
0.696CysSer: 0.696 ± 0.207
0.348CysThr: 0.348 ± 0.175
0.279CysVal: 0.279 ± 0.172
0.279CysTrp: 0.279 ± 0.128
0.418CysTyr: 0.418 ± 0.181
0.0CysXaa: 0.0 ± 0.0
Asp
6.545AspAla: 6.545 ± 0.531
0.696AspCys: 0.696 ± 0.238
3.83AspAsp: 3.83 ± 0.724
5.988AspGlu: 5.988 ± 0.803
2.228AspPhe: 2.228 ± 0.37
4.735AspGly: 4.735 ± 0.662
1.253AspHis: 1.253 ± 0.308
3.064AspIle: 3.064 ± 0.41
2.855AspLys: 2.855 ± 0.28
5.918AspLeu: 5.918 ± 0.776
1.741AspMet: 1.741 ± 0.345
1.532AspAsn: 1.532 ± 0.295
3.969AspPro: 3.969 ± 0.502
2.298AspGln: 2.298 ± 0.375
3.621AspArg: 3.621 ± 0.448
4.178AspSer: 4.178 ± 0.646
2.646AspThr: 2.646 ± 0.457
3.412AspVal: 3.412 ± 0.623
1.184AspTrp: 1.184 ± 0.29
2.715AspTyr: 2.715 ± 0.517
0.0AspXaa: 0.0 ± 0.0
Glu
5.501GluAla: 5.501 ± 0.665
0.627GluCys: 0.627 ± 0.224
6.267GluAsp: 6.267 ± 1.035
4.526GluGlu: 4.526 ± 0.864
1.671GluPhe: 1.671 ± 0.279
3.76GluGly: 3.76 ± 0.381
1.601GluHis: 1.601 ± 0.418
2.785GluIle: 2.785 ± 0.492
2.158GluLys: 2.158 ± 0.576
5.152GluLeu: 5.152 ± 0.772
2.019GluMet: 2.019 ± 0.419
1.81GluAsn: 1.81 ± 0.323
2.855GluPro: 2.855 ± 0.426
2.646GluGln: 2.646 ± 0.486
4.944GluArg: 4.944 ± 0.93
3.273GluSer: 3.273 ± 0.466
2.924GluThr: 2.924 ± 0.407
4.387GluVal: 4.387 ± 0.743
1.393GluTrp: 1.393 ± 0.292
2.298GluTyr: 2.298 ± 0.499
0.0GluXaa: 0.0 ± 0.0
Phe
2.785PheAla: 2.785 ± 0.446
0.418PheCys: 0.418 ± 0.17
2.576PheAsp: 2.576 ± 0.445
1.532PheGlu: 1.532 ± 0.415
1.044PhePhe: 1.044 ± 0.269
2.019PheGly: 2.019 ± 0.408
0.696PheHis: 0.696 ± 0.197
2.437PheIle: 2.437 ± 0.372
1.81PheLys: 1.81 ± 0.492
1.81PheLeu: 1.81 ± 0.319
0.696PheMet: 0.696 ± 0.214
1.393PheAsn: 1.393 ± 0.243
1.323PhePro: 1.323 ± 0.35
0.975PheGln: 0.975 ± 0.227
2.507PheArg: 2.507 ± 0.495
2.298PheSer: 2.298 ± 0.647
2.715PheThr: 2.715 ± 0.488
2.089PheVal: 2.089 ± 0.342
0.836PheTrp: 0.836 ± 0.183
0.557PheTyr: 0.557 ± 0.224
0.0PheXaa: 0.0 ± 0.0
Gly
7.102GlyAla: 7.102 ± 0.954
0.696GlyCys: 0.696 ± 0.281
6.127GlyAsp: 6.127 ± 0.766
4.108GlyGlu: 4.108 ± 0.554
3.342GlyPhe: 3.342 ± 0.464
6.197GlyGly: 6.197 ± 0.91
1.393GlyHis: 1.393 ± 0.358
3.83GlyIle: 3.83 ± 0.559
4.595GlyLys: 4.595 ± 0.592
4.944GlyLeu: 4.944 ± 0.713
2.715GlyMet: 2.715 ± 0.417
2.367GlyAsn: 2.367 ± 0.359
2.924GlyPro: 2.924 ± 0.583
3.481GlyGln: 3.481 ± 0.565
5.083GlyArg: 5.083 ± 0.611
5.222GlySer: 5.222 ± 0.99
5.501GlyThr: 5.501 ± 0.803
5.64GlyVal: 5.64 ± 0.605
1.741GlyTrp: 1.741 ± 0.426
3.133GlyTyr: 3.133 ± 0.529
0.0GlyXaa: 0.0 ± 0.0
His
1.532HisAla: 1.532 ± 0.244
0.139HisCys: 0.139 ± 0.099
1.462HisAsp: 1.462 ± 0.328
1.393HisGlu: 1.393 ± 0.373
0.696HisPhe: 0.696 ± 0.285
1.044HisGly: 1.044 ± 0.331
0.557HisHis: 0.557 ± 0.233
0.766HisIle: 0.766 ± 0.269
0.766HisLys: 0.766 ± 0.209
1.81HisLeu: 1.81 ± 0.425
0.836HisMet: 0.836 ± 0.266
0.975HisAsn: 0.975 ± 0.208
1.184HisPro: 1.184 ± 0.279
0.836HisGln: 0.836 ± 0.265
1.253HisArg: 1.253 ± 0.317
1.253HisSer: 1.253 ± 0.329
1.671HisThr: 1.671 ± 0.328
0.766HisVal: 0.766 ± 0.201
0.279HisTrp: 0.279 ± 0.126
0.487HisTyr: 0.487 ± 0.196
0.0HisXaa: 0.0 ± 0.0
Ile
5.501IleAla: 5.501 ± 0.652
0.07IleCys: 0.07 ± 0.07
3.76IleAsp: 3.76 ± 0.456
3.342IleGlu: 3.342 ± 0.48
1.253IlePhe: 1.253 ± 0.314
3.969IleGly: 3.969 ± 0.835
0.836IleHis: 0.836 ± 0.237
2.576IleIle: 2.576 ± 0.52
2.576IleLys: 2.576 ± 0.569
2.715IleLeu: 2.715 ± 0.422
0.696IleMet: 0.696 ± 0.17
1.462IleAsn: 1.462 ± 0.345
3.412IlePro: 3.412 ± 0.635
1.323IleGln: 1.323 ± 0.288
3.76IleArg: 3.76 ± 0.541
3.342IleSer: 3.342 ± 0.472
3.064IleThr: 3.064 ± 0.54
2.994IleVal: 2.994 ± 0.365
0.836IleTrp: 0.836 ± 0.258
1.253IleTyr: 1.253 ± 0.348
0.0IleXaa: 0.0 ± 0.0
Lys
5.849LysAla: 5.849 ± 0.712
0.279LysCys: 0.279 ± 0.172
2.994LysAsp: 2.994 ± 0.332
3.481LysGlu: 3.481 ± 0.572
1.81LysPhe: 1.81 ± 0.423
4.526LysGly: 4.526 ± 0.48
0.905LysHis: 0.905 ± 0.259
1.671LysIle: 1.671 ± 0.358
2.785LysLys: 2.785 ± 0.475
5.152LysLeu: 5.152 ± 0.543
1.462LysMet: 1.462 ± 0.375
1.95LysAsn: 1.95 ± 0.376
2.576LysPro: 2.576 ± 0.494
2.228LysGln: 2.228 ± 0.496
3.133LysArg: 3.133 ± 0.559
2.785LysSer: 2.785 ± 0.459
2.715LysThr: 2.715 ± 0.421
2.715LysVal: 2.715 ± 0.578
1.253LysTrp: 1.253 ± 0.347
1.184LysTyr: 1.184 ± 0.309
0.0LysXaa: 0.0 ± 0.0
Leu
8.146LeuAla: 8.146 ± 0.894
0.696LeuCys: 0.696 ± 0.321
4.874LeuAsp: 4.874 ± 0.642
4.874LeuGlu: 4.874 ± 0.764
1.323LeuPhe: 1.323 ± 0.513
6.058LeuGly: 6.058 ± 0.84
1.393LeuHis: 1.393 ± 0.296
3.621LeuIle: 3.621 ± 0.551
5.431LeuLys: 5.431 ± 0.603
7.311LeuLeu: 7.311 ± 0.774
2.228LeuMet: 2.228 ± 0.354
2.924LeuAsn: 2.924 ± 0.499
4.595LeuPro: 4.595 ± 0.591
2.507LeuGln: 2.507 ± 0.387
6.684LeuArg: 6.684 ± 0.736
5.083LeuSer: 5.083 ± 0.636
4.178LeuThr: 4.178 ± 0.492
5.64LeuVal: 5.64 ± 0.605
1.601LeuTrp: 1.601 ± 0.303
2.228LeuTyr: 2.228 ± 0.353
0.0LeuXaa: 0.0 ± 0.0
Met
3.273MetAla: 3.273 ± 0.47
0.139MetCys: 0.139 ± 0.102
1.253MetAsp: 1.253 ± 0.29
1.671MetGlu: 1.671 ± 0.371
1.114MetPhe: 1.114 ± 0.294
1.741MetGly: 1.741 ± 0.438
0.418MetHis: 0.418 ± 0.174
2.298MetIle: 2.298 ± 0.435
1.323MetLys: 1.323 ± 0.407
2.228MetLeu: 2.228 ± 0.419
0.696MetMet: 0.696 ± 0.159
1.044MetAsn: 1.044 ± 0.296
1.462MetPro: 1.462 ± 0.298
0.975MetGln: 0.975 ± 0.266
1.253MetArg: 1.253 ± 0.23
2.228MetSer: 2.228 ± 0.466
1.741MetThr: 1.741 ± 0.27
1.88MetVal: 1.88 ± 0.363
0.766MetTrp: 0.766 ± 0.297
0.348MetTyr: 0.348 ± 0.131
0.0MetXaa: 0.0 ± 0.0
Asn
3.342AsnAla: 3.342 ± 0.644
0.418AsnCys: 0.418 ± 0.177
1.671AsnAsp: 1.671 ± 0.396
1.741AsnGlu: 1.741 ± 0.416
1.393AsnPhe: 1.393 ± 0.436
3.481AsnGly: 3.481 ± 0.593
0.418AsnHis: 0.418 ± 0.178
0.975AsnIle: 0.975 ± 0.408
1.601AsnLys: 1.601 ± 0.361
1.95AsnLeu: 1.95 ± 0.386
0.696AsnMet: 0.696 ± 0.24
0.905AsnAsn: 0.905 ± 0.274
3.83AsnPro: 3.83 ± 0.665
0.975AsnGln: 0.975 ± 0.268
2.437AsnArg: 2.437 ± 0.485
1.95AsnSer: 1.95 ± 0.424
1.601AsnThr: 1.601 ± 0.33
2.158AsnVal: 2.158 ± 0.386
0.905AsnTrp: 0.905 ± 0.249
0.766AsnTyr: 0.766 ± 0.213
0.0AsnXaa: 0.0 ± 0.0
Pro
4.595ProAla: 4.595 ± 0.615
0.557ProCys: 0.557 ± 0.227
3.69ProAsp: 3.69 ± 0.488
3.064ProGlu: 3.064 ± 0.457
1.532ProPhe: 1.532 ± 0.389
5.361ProGly: 5.361 ± 0.677
1.393ProHis: 1.393 ± 0.332
2.089ProIle: 2.089 ± 0.428
3.133ProLys: 3.133 ± 0.586
3.899ProLeu: 3.899 ± 0.693
1.601ProMet: 1.601 ± 0.302
1.81ProAsn: 1.81 ± 0.399
2.367ProPro: 2.367 ± 0.49
1.671ProGln: 1.671 ± 0.323
2.576ProArg: 2.576 ± 0.401
3.76ProSer: 3.76 ± 0.464
3.133ProThr: 3.133 ± 0.537
3.83ProVal: 3.83 ± 0.56
1.044ProTrp: 1.044 ± 0.242
1.114ProTyr: 1.114 ± 0.267
0.0ProXaa: 0.0 ± 0.0
Gln
3.412GlnAla: 3.412 ± 0.4
0.418GlnCys: 0.418 ± 0.204
2.019GlnAsp: 2.019 ± 0.394
2.228GlnGlu: 2.228 ± 0.427
1.741GlnPhe: 1.741 ± 0.378
1.95GlnGly: 1.95 ± 0.353
0.836GlnHis: 0.836 ± 0.273
2.089GlnIle: 2.089 ± 0.396
1.671GlnLys: 1.671 ± 0.355
2.855GlnLeu: 2.855 ± 0.354
0.836GlnMet: 0.836 ± 0.271
1.184GlnAsn: 1.184 ± 0.321
2.019GlnPro: 2.019 ± 0.418
1.393GlnGln: 1.393 ± 0.395
2.785GlnArg: 2.785 ± 0.417
1.88GlnSer: 1.88 ± 0.295
1.741GlnThr: 1.741 ± 0.317
2.576GlnVal: 2.576 ± 0.387
0.766GlnTrp: 0.766 ± 0.19
1.393GlnTyr: 1.393 ± 0.345
0.0GlnXaa: 0.0 ± 0.0
Arg
5.988ArgAla: 5.988 ± 0.778
0.418ArgCys: 0.418 ± 0.194
4.456ArgAsp: 4.456 ± 0.614
4.178ArgGlu: 4.178 ± 0.483
2.228ArgPhe: 2.228 ± 0.436
5.431ArgGly: 5.431 ± 0.676
1.044ArgHis: 1.044 ± 0.266
2.715ArgIle: 2.715 ± 0.441
4.178ArgLys: 4.178 ± 0.513
5.083ArgLeu: 5.083 ± 0.643
1.95ArgMet: 1.95 ± 0.445
2.019ArgAsn: 2.019 ± 0.344
2.785ArgPro: 2.785 ± 0.434
3.203ArgGln: 3.203 ± 0.486
4.108ArgArg: 4.108 ± 0.581
3.133ArgSer: 3.133 ± 0.477
3.133ArgThr: 3.133 ± 0.435
5.013ArgVal: 5.013 ± 0.859
1.601ArgTrp: 1.601 ± 0.427
1.88ArgTyr: 1.88 ± 0.365
0.0ArgXaa: 0.0 ± 0.0
Ser
5.361SerAla: 5.361 ± 0.651
0.487SerCys: 0.487 ± 0.191
3.203SerAsp: 3.203 ± 0.527
3.969SerGlu: 3.969 ± 0.765
1.741SerPhe: 1.741 ± 0.297
6.684SerGly: 6.684 ± 0.609
1.253SerHis: 1.253 ± 0.37
3.69SerIle: 3.69 ± 0.683
2.298SerLys: 2.298 ± 0.424
5.57SerLeu: 5.57 ± 0.794
1.95SerMet: 1.95 ± 0.348
1.671SerAsn: 1.671 ± 0.312
3.412SerPro: 3.412 ± 0.559
2.298SerGln: 2.298 ± 0.464
3.273SerArg: 3.273 ± 0.419
4.178SerSer: 4.178 ± 0.492
3.899SerThr: 3.899 ± 0.528
3.899SerVal: 3.899 ± 0.52
1.462SerTrp: 1.462 ± 0.401
1.323SerTyr: 1.323 ± 0.317
0.0SerXaa: 0.0 ± 0.0
Thr
6.058ThrAla: 6.058 ± 0.867
0.209ThrCys: 0.209 ± 0.129
3.064ThrAsp: 3.064 ± 0.702
2.298ThrGlu: 2.298 ± 0.417
1.532ThrPhe: 1.532 ± 0.256
5.64ThrGly: 5.64 ± 0.71
1.253ThrHis: 1.253 ± 0.297
2.924ThrIle: 2.924 ± 0.481
3.899ThrLys: 3.899 ± 0.539
4.804ThrLeu: 4.804 ± 0.589
1.601ThrMet: 1.601 ± 0.302
2.089ThrAsn: 2.089 ± 0.383
3.69ThrPro: 3.69 ± 0.435
1.741ThrGln: 1.741 ± 0.319
3.621ThrArg: 3.621 ± 0.541
2.646ThrSer: 2.646 ± 0.438
4.317ThrThr: 4.317 ± 0.625
4.804ThrVal: 4.804 ± 0.647
0.905ThrTrp: 0.905 ± 0.212
1.671ThrTyr: 1.671 ± 0.333
0.0ThrXaa: 0.0 ± 0.0
Val
6.615ValAla: 6.615 ± 0.585
0.487ValCys: 0.487 ± 0.189
3.551ValAsp: 3.551 ± 0.408
4.456ValGlu: 4.456 ± 0.574
2.089ValPhe: 2.089 ± 0.383
5.222ValGly: 5.222 ± 0.799
1.044ValHis: 1.044 ± 0.299
3.969ValIle: 3.969 ± 0.675
3.342ValLys: 3.342 ± 0.453
5.431ValLeu: 5.431 ± 0.619
1.88ValMet: 1.88 ± 0.34
2.715ValAsn: 2.715 ± 0.468
2.855ValPro: 2.855 ± 0.469
2.715ValGln: 2.715 ± 0.401
3.551ValArg: 3.551 ± 0.554
4.178ValSer: 4.178 ± 0.564
5.083ValThr: 5.083 ± 0.605
4.456ValVal: 4.456 ± 0.588
2.089ValTrp: 2.089 ± 0.267
2.228ValTyr: 2.228 ± 0.485
0.0ValXaa: 0.0 ± 0.0
Trp
2.019TrpAla: 2.019 ± 0.387
0.418TrpCys: 0.418 ± 0.2
1.393TrpAsp: 1.393 ± 0.323
1.462TrpGlu: 1.462 ± 0.301
0.696TrpPhe: 0.696 ± 0.197
1.184TrpGly: 1.184 ± 0.43
0.696TrpHis: 0.696 ± 0.228
0.487TrpIle: 0.487 ± 0.182
1.184TrpLys: 1.184 ± 0.282
2.158TrpLeu: 2.158 ± 0.355
0.487TrpMet: 0.487 ± 0.224
1.323TrpAsn: 1.323 ± 0.445
0.905TrpPro: 0.905 ± 0.282
1.393TrpGln: 1.393 ± 0.283
1.462TrpArg: 1.462 ± 0.295
1.184TrpSer: 1.184 ± 0.257
0.627TrpThr: 0.627 ± 0.187
2.089TrpVal: 2.089 ± 0.345
0.418TrpTrp: 0.418 ± 0.178
0.766TrpTyr: 0.766 ± 0.201
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.158TyrAla: 2.158 ± 0.38
0.209TyrCys: 0.209 ± 0.118
2.019TyrAsp: 2.019 ± 0.412
1.95TyrGlu: 1.95 ± 0.421
0.975TyrPhe: 0.975 ± 0.265
2.576TyrGly: 2.576 ± 0.421
0.836TyrHis: 0.836 ± 0.362
1.114TyrIle: 1.114 ± 0.321
1.253TyrLys: 1.253 ± 0.314
2.576TyrLeu: 2.576 ± 0.477
1.253TyrMet: 1.253 ± 0.304
1.044TyrAsn: 1.044 ± 0.201
1.253TyrPro: 1.253 ± 0.296
0.696TyrGln: 0.696 ± 0.204
2.367TyrArg: 2.367 ± 0.471
2.019TyrSer: 2.019 ± 0.393
1.462TyrThr: 1.462 ± 0.363
2.576TyrVal: 2.576 ± 0.359
0.279TyrTrp: 0.279 ± 0.147
0.696TyrTyr: 0.696 ± 0.221
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 67 proteins (14363 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski