Amino acid dipepetide frequency for Mycobacterium phage Cornucopia

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
13.781AlaAla: 13.781 ± 1.431
1.186AlaCys: 1.186 ± 0.299
6.721AlaAsp: 6.721 ± 0.658
8.529AlaGlu: 8.529 ± 0.876
2.768AlaPhe: 2.768 ± 0.399
9.884AlaGly: 9.884 ± 1.303
2.485AlaHis: 2.485 ± 0.439
4.462AlaIle: 4.462 ± 0.44
4.293AlaLys: 4.293 ± 0.488
8.924AlaLeu: 8.924 ± 0.731
2.711AlaMet: 2.711 ± 0.36
2.881AlaAsn: 2.881 ± 0.454
4.801AlaPro: 4.801 ± 0.554
3.671AlaGln: 3.671 ± 0.516
6.778AlaArg: 6.778 ± 0.717
5.705AlaSer: 5.705 ± 0.67
5.874AlaThr: 5.874 ± 0.615
6.721AlaVal: 6.721 ± 0.666
2.316AlaTrp: 2.316 ± 0.301
2.259AlaTyr: 2.259 ± 0.308
0.0AlaXaa: 0.0 ± 0.0
Cys
1.017CysAla: 1.017 ± 0.329
0.169CysCys: 0.169 ± 0.093
1.017CysAsp: 1.017 ± 0.266
0.904CysGlu: 0.904 ± 0.299
0.282CysPhe: 0.282 ± 0.104
1.977CysGly: 1.977 ± 0.4
0.113CysHis: 0.113 ± 0.087
0.282CysIle: 0.282 ± 0.137
0.452CysLys: 0.452 ± 0.175
0.791CysLeu: 0.791 ± 0.233
0.169CysMet: 0.169 ± 0.102
0.508CysAsn: 0.508 ± 0.173
1.299CysPro: 1.299 ± 0.332
0.395CysGln: 0.395 ± 0.148
0.791CysArg: 0.791 ± 0.266
0.678CysSer: 0.678 ± 0.241
0.734CysThr: 0.734 ± 0.248
0.734CysVal: 0.734 ± 0.224
0.226CysTrp: 0.226 ± 0.094
0.169CysTyr: 0.169 ± 0.091
0.0CysXaa: 0.0 ± 0.0
Asp
6.947AspAla: 6.947 ± 0.641
0.847AspCys: 0.847 ± 0.264
4.688AspAsp: 4.688 ± 0.515
3.163AspGlu: 3.163 ± 0.47
1.186AspPhe: 1.186 ± 0.234
6.326AspGly: 6.326 ± 0.552
1.243AspHis: 1.243 ± 0.226
2.994AspIle: 2.994 ± 0.425
1.751AspLys: 1.751 ± 0.297
6.043AspLeu: 6.043 ± 0.609
1.356AspMet: 1.356 ± 0.271
1.581AspAsn: 1.581 ± 0.362
4.97AspPro: 4.97 ± 0.661
2.768AspGln: 2.768 ± 0.309
5.083AspArg: 5.083 ± 0.627
3.106AspSer: 3.106 ± 0.505
3.954AspThr: 3.954 ± 0.473
4.631AspVal: 4.631 ± 0.621
1.751AspTrp: 1.751 ± 0.277
1.864AspTyr: 1.864 ± 0.331
0.0AspXaa: 0.0 ± 0.0
Glu
6.382GluAla: 6.382 ± 0.542
1.017GluCys: 1.017 ± 0.265
3.219GluAsp: 3.219 ± 0.393
2.259GluGlu: 2.259 ± 0.387
2.259GluPhe: 2.259 ± 0.319
2.768GluGly: 2.768 ± 0.432
1.243GluHis: 1.243 ± 0.314
2.203GluIle: 2.203 ± 0.42
1.694GluLys: 1.694 ± 0.31
4.97GluLeu: 4.97 ± 0.691
1.694GluMet: 1.694 ± 0.3
1.807GluAsn: 1.807 ± 0.248
2.881GluPro: 2.881 ± 0.363
3.163GluGln: 3.163 ± 0.455
5.196GluArg: 5.196 ± 0.607
3.276GluSer: 3.276 ± 0.492
3.784GluThr: 3.784 ± 0.544
4.01GluVal: 4.01 ± 0.579
1.243GluTrp: 1.243 ± 0.239
1.525GluTyr: 1.525 ± 0.328
0.0GluXaa: 0.0 ± 0.0
Phe
2.994PheAla: 2.994 ± 0.34
0.282PheCys: 0.282 ± 0.134
2.542PheAsp: 2.542 ± 0.394
1.412PheGlu: 1.412 ± 0.295
0.96PhePhe: 0.96 ± 0.264
3.163PheGly: 3.163 ± 0.774
0.339PheHis: 0.339 ± 0.143
1.525PheIle: 1.525 ± 0.298
1.13PheLys: 1.13 ± 0.276
1.751PheLeu: 1.751 ± 0.322
0.96PheMet: 0.96 ± 0.246
1.186PheAsn: 1.186 ± 0.355
1.581PhePro: 1.581 ± 0.32
0.96PheGln: 0.96 ± 0.309
1.638PheArg: 1.638 ± 0.301
1.638PheSer: 1.638 ± 0.263
2.033PheThr: 2.033 ± 0.42
1.581PheVal: 1.581 ± 0.272
0.678PheTrp: 0.678 ± 0.201
1.017PheTyr: 1.017 ± 0.264
0.0PheXaa: 0.0 ± 0.0
Gly
9.376GlyAla: 9.376 ± 1.065
1.017GlyCys: 1.017 ± 0.263
6.439GlyAsp: 6.439 ± 0.527
3.445GlyGlu: 3.445 ± 0.429
2.768GlyPhe: 2.768 ± 0.43
10.449GlyGly: 10.449 ± 1.952
1.694GlyHis: 1.694 ± 0.258
4.067GlyIle: 4.067 ± 0.581
2.768GlyLys: 2.768 ± 0.323
5.931GlyLeu: 5.931 ± 0.548
2.372GlyMet: 2.372 ± 0.412
3.332GlyAsn: 3.332 ± 0.381
3.841GlyPro: 3.841 ± 0.499
2.598GlyGln: 2.598 ± 0.737
5.14GlyArg: 5.14 ± 0.589
5.818GlySer: 5.818 ± 1.013
6.947GlyThr: 6.947 ± 0.779
5.931GlyVal: 5.931 ± 0.622
2.711GlyTrp: 2.711 ± 0.395
2.372GlyTyr: 2.372 ± 0.401
0.0GlyXaa: 0.0 ± 0.0
His
1.694HisAla: 1.694 ± 0.36
0.508HisCys: 0.508 ± 0.225
1.243HisAsp: 1.243 ± 0.28
1.243HisGlu: 1.243 ± 0.252
0.508HisPhe: 0.508 ± 0.17
1.751HisGly: 1.751 ± 0.286
1.13HisHis: 1.13 ± 0.261
1.469HisIle: 1.469 ± 0.301
0.734HisLys: 0.734 ± 0.208
1.807HisLeu: 1.807 ± 0.313
0.565HisMet: 0.565 ± 0.168
0.678HisAsn: 0.678 ± 0.202
1.412HisPro: 1.412 ± 0.257
0.791HisGln: 0.791 ± 0.223
1.977HisArg: 1.977 ± 0.418
0.791HisSer: 0.791 ± 0.191
1.412HisThr: 1.412 ± 0.322
1.525HisVal: 1.525 ± 0.293
0.508HisTrp: 0.508 ± 0.176
0.791HisTyr: 0.791 ± 0.236
0.0HisXaa: 0.0 ± 0.0
Ile
5.479IleAla: 5.479 ± 0.613
0.734IleCys: 0.734 ± 0.253
3.671IleAsp: 3.671 ± 0.489
3.219IleGlu: 3.219 ± 0.39
0.678IlePhe: 0.678 ± 0.268
3.784IleGly: 3.784 ± 0.487
1.243IleHis: 1.243 ± 0.271
1.469IleIle: 1.469 ± 0.306
1.469IleLys: 1.469 ± 0.308
2.316IleLeu: 2.316 ± 0.373
0.452IleMet: 0.452 ± 0.144
1.807IleAsn: 1.807 ± 0.283
2.881IlePro: 2.881 ± 0.331
1.581IleGln: 1.581 ± 0.265
2.711IleArg: 2.711 ± 0.477
2.485IleSer: 2.485 ± 0.461
3.728IleThr: 3.728 ± 0.414
3.276IleVal: 3.276 ± 0.414
0.904IleTrp: 0.904 ± 0.249
0.734IleTyr: 0.734 ± 0.207
0.0IleXaa: 0.0 ± 0.0
Lys
3.615LysAla: 3.615 ± 0.499
0.339LysCys: 0.339 ± 0.14
2.09LysAsp: 2.09 ± 0.333
1.469LysGlu: 1.469 ± 0.31
1.356LysPhe: 1.356 ± 0.207
2.768LysGly: 2.768 ± 0.387
1.13LysHis: 1.13 ± 0.249
0.96LysIle: 0.96 ± 0.261
1.299LysLys: 1.299 ± 0.284
3.05LysLeu: 3.05 ± 0.541
0.678LysMet: 0.678 ± 0.197
0.96LysAsn: 0.96 ± 0.223
2.429LysPro: 2.429 ± 0.424
1.581LysGln: 1.581 ± 0.236
2.203LysArg: 2.203 ± 0.397
1.807LysSer: 1.807 ± 0.355
2.033LysThr: 2.033 ± 0.354
1.977LysVal: 1.977 ± 0.342
0.452LysTrp: 0.452 ± 0.156
1.073LysTyr: 1.073 ± 0.248
0.0LysXaa: 0.0 ± 0.0
Leu
8.303LeuAla: 8.303 ± 0.867
0.734LeuCys: 0.734 ± 0.3
5.14LeuAsp: 5.14 ± 0.554
4.01LeuGlu: 4.01 ± 0.501
1.92LeuPhe: 1.92 ± 0.272
5.987LeuGly: 5.987 ± 0.512
1.13LeuHis: 1.13 ± 0.344
3.389LeuIle: 3.389 ± 0.403
2.203LeuLys: 2.203 ± 0.403
5.083LeuLeu: 5.083 ± 0.622
1.694LeuMet: 1.694 ± 0.338
2.768LeuAsn: 2.768 ± 0.392
5.535LeuPro: 5.535 ± 0.607
2.768LeuGln: 2.768 ± 0.489
5.14LeuArg: 5.14 ± 0.695
5.309LeuSer: 5.309 ± 0.514
5.479LeuThr: 5.479 ± 0.578
5.253LeuVal: 5.253 ± 0.604
1.073LeuTrp: 1.073 ± 0.281
1.864LeuTyr: 1.864 ± 0.389
0.0LeuXaa: 0.0 ± 0.0
Met
1.864MetAla: 1.864 ± 0.399
0.282MetCys: 0.282 ± 0.209
1.186MetAsp: 1.186 ± 0.29
0.847MetGlu: 0.847 ± 0.201
1.017MetPhe: 1.017 ± 0.255
1.92MetGly: 1.92 ± 0.302
0.226MetHis: 0.226 ± 0.109
0.904MetIle: 0.904 ± 0.201
0.791MetLys: 0.791 ± 0.241
1.638MetLeu: 1.638 ± 0.219
0.508MetMet: 0.508 ± 0.205
0.904MetAsn: 0.904 ± 0.233
1.243MetPro: 1.243 ± 0.276
0.339MetGln: 0.339 ± 0.157
1.638MetArg: 1.638 ± 0.293
2.881MetSer: 2.881 ± 0.396
2.259MetThr: 2.259 ± 0.415
1.469MetVal: 1.469 ± 0.35
0.508MetTrp: 0.508 ± 0.19
0.339MetTyr: 0.339 ± 0.151
0.0MetXaa: 0.0 ± 0.0
Asn
3.728AsnAla: 3.728 ± 0.496
0.226AsnCys: 0.226 ± 0.11
1.864AsnAsp: 1.864 ± 0.327
1.751AsnGlu: 1.751 ± 0.326
0.847AsnPhe: 0.847 ± 0.297
4.406AsnGly: 4.406 ± 0.555
0.96AsnHis: 0.96 ± 0.224
1.525AsnIle: 1.525 ± 0.485
1.13AsnLys: 1.13 ± 0.235
2.316AsnLeu: 2.316 ± 0.343
0.734AsnMet: 0.734 ± 0.181
1.186AsnAsn: 1.186 ± 0.313
2.372AsnPro: 2.372 ± 0.327
1.073AsnGln: 1.073 ± 0.31
2.429AsnArg: 2.429 ± 0.377
1.638AsnSer: 1.638 ± 0.284
2.203AsnThr: 2.203 ± 0.359
1.581AsnVal: 1.581 ± 0.254
0.678AsnTrp: 0.678 ± 0.25
0.395AsnTyr: 0.395 ± 0.134
0.0AsnXaa: 0.0 ± 0.0
Pro
6.269ProAla: 6.269 ± 0.689
0.678ProCys: 0.678 ± 0.207
3.897ProAsp: 3.897 ± 0.482
4.067ProGlu: 4.067 ± 0.457
1.864ProPhe: 1.864 ± 0.311
6.439ProGly: 6.439 ± 0.632
1.581ProHis: 1.581 ± 0.302
2.485ProIle: 2.485 ± 0.358
2.033ProLys: 2.033 ± 0.363
3.728ProLeu: 3.728 ± 0.514
1.243ProMet: 1.243 ± 0.285
2.033ProAsn: 2.033 ± 0.304
3.897ProPro: 3.897 ± 0.622
2.316ProGln: 2.316 ± 0.441
2.881ProArg: 2.881 ± 0.537
3.106ProSer: 3.106 ± 0.448
3.106ProThr: 3.106 ± 0.427
5.253ProVal: 5.253 ± 0.609
0.904ProTrp: 0.904 ± 0.228
1.92ProTyr: 1.92 ± 0.325
0.0ProXaa: 0.0 ± 0.0
Gln
4.518GlnAla: 4.518 ± 0.69
0.452GlnCys: 0.452 ± 0.189
1.412GlnAsp: 1.412 ± 0.263
1.92GlnGlu: 1.92 ± 0.341
1.13GlnPhe: 1.13 ± 0.321
2.372GlnGly: 2.372 ± 0.453
0.904GlnHis: 0.904 ± 0.225
2.09GlnIle: 2.09 ± 0.336
1.412GlnLys: 1.412 ± 0.256
3.558GlnLeu: 3.558 ± 0.452
0.734GlnMet: 0.734 ± 0.203
0.734GlnAsn: 0.734 ± 0.243
2.372GlnPro: 2.372 ± 0.441
1.977GlnGln: 1.977 ± 0.422
2.146GlnArg: 2.146 ± 0.338
2.316GlnSer: 2.316 ± 0.364
1.694GlnThr: 1.694 ± 0.351
2.316GlnVal: 2.316 ± 0.406
0.96GlnTrp: 0.96 ± 0.244
1.073GlnTyr: 1.073 ± 0.297
0.0GlnXaa: 0.0 ± 0.0
Arg
6.778ArgAla: 6.778 ± 0.757
1.412ArgCys: 1.412 ± 0.355
4.349ArgAsp: 4.349 ± 0.662
4.744ArgGlu: 4.744 ± 0.632
2.316ArgPhe: 2.316 ± 0.427
3.558ArgGly: 3.558 ± 0.387
1.638ArgHis: 1.638 ± 0.343
3.615ArgIle: 3.615 ± 0.466
2.146ArgLys: 2.146 ± 0.384
4.744ArgLeu: 4.744 ± 0.628
2.259ArgMet: 2.259 ± 0.406
2.203ArgAsn: 2.203 ± 0.418
3.332ArgPro: 3.332 ± 0.483
2.033ArgGln: 2.033 ± 0.337
5.14ArgArg: 5.14 ± 0.727
3.954ArgSer: 3.954 ± 0.557
3.389ArgThr: 3.389 ± 0.535
5.083ArgVal: 5.083 ± 0.626
1.751ArgTrp: 1.751 ± 0.319
1.977ArgTyr: 1.977 ± 0.308
0.0ArgXaa: 0.0 ± 0.0
Ser
5.648SerAla: 5.648 ± 0.624
0.282SerCys: 0.282 ± 0.124
4.067SerAsp: 4.067 ± 0.48
3.276SerGlu: 3.276 ± 0.559
2.203SerPhe: 2.203 ± 0.463
7.23SerGly: 7.23 ± 1.07
1.13SerHis: 1.13 ± 0.229
2.881SerIle: 2.881 ± 0.42
2.429SerLys: 2.429 ± 0.375
3.671SerLeu: 3.671 ± 0.564
1.073SerMet: 1.073 ± 0.269
2.372SerAsn: 2.372 ± 0.485
3.502SerPro: 3.502 ± 0.318
1.638SerGln: 1.638 ± 0.298
3.558SerArg: 3.558 ± 0.404
4.631SerSer: 4.631 ± 0.701
2.937SerThr: 2.937 ± 0.41
4.914SerVal: 4.914 ± 0.62
1.469SerTrp: 1.469 ± 0.279
1.525SerTyr: 1.525 ± 0.25
0.0SerXaa: 0.0 ± 0.0
Thr
5.931ThrAla: 5.931 ± 0.678
0.621ThrCys: 0.621 ± 0.198
3.558ThrAsp: 3.558 ± 0.499
3.389ThrGlu: 3.389 ± 0.4
1.694ThrPhe: 1.694 ± 0.357
5.592ThrGly: 5.592 ± 0.57
1.694ThrHis: 1.694 ± 0.286
3.276ThrIle: 3.276 ± 0.461
2.09ThrLys: 2.09 ± 0.335
5.083ThrLeu: 5.083 ± 0.554
1.017ThrMet: 1.017 ± 0.218
2.09ThrAsn: 2.09 ± 0.358
4.857ThrPro: 4.857 ± 0.513
2.09ThrGln: 2.09 ± 0.319
3.841ThrArg: 3.841 ± 0.423
3.615ThrSer: 3.615 ± 0.489
4.688ThrThr: 4.688 ± 0.637
6.326ThrVal: 6.326 ± 0.748
1.13ThrTrp: 1.13 ± 0.255
1.977ThrTyr: 1.977 ± 0.323
0.0ThrXaa: 0.0 ± 0.0
Val
8.077ValAla: 8.077 ± 0.755
1.356ValCys: 1.356 ± 0.296
5.931ValAsp: 5.931 ± 0.618
4.236ValGlu: 4.236 ± 0.507
2.259ValPhe: 2.259 ± 0.408
5.592ValGly: 5.592 ± 0.594
1.581ValHis: 1.581 ± 0.316
2.881ValIle: 2.881 ± 0.428
1.92ValLys: 1.92 ± 0.317
5.422ValLeu: 5.422 ± 0.714
1.186ValMet: 1.186 ± 0.223
2.598ValAsn: 2.598 ± 0.365
4.01ValPro: 4.01 ± 0.342
2.485ValGln: 2.485 ± 0.371
4.518ValArg: 4.518 ± 0.653
5.027ValSer: 5.027 ± 0.556
5.083ValThr: 5.083 ± 0.471
5.705ValVal: 5.705 ± 0.556
1.638ValTrp: 1.638 ± 0.311
1.412ValTyr: 1.412 ± 0.371
0.0ValXaa: 0.0 ± 0.0
Trp
1.751TrpAla: 1.751 ± 0.242
0.226TrpCys: 0.226 ± 0.107
1.356TrpAsp: 1.356 ± 0.289
1.017TrpGlu: 1.017 ± 0.26
0.621TrpPhe: 0.621 ± 0.163
0.621TrpGly: 0.621 ± 0.19
0.565TrpHis: 0.565 ± 0.165
1.13TrpIle: 1.13 ± 0.231
0.791TrpLys: 0.791 ± 0.192
1.807TrpLeu: 1.807 ± 0.412
1.017TrpMet: 1.017 ± 0.245
0.678TrpAsn: 0.678 ± 0.208
1.186TrpPro: 1.186 ± 0.275
1.073TrpGln: 1.073 ± 0.258
1.751TrpArg: 1.751 ± 0.371
1.638TrpSer: 1.638 ± 0.29
1.525TrpThr: 1.525 ± 0.284
2.09TrpVal: 2.09 ± 0.452
0.96TrpTrp: 0.96 ± 0.196
0.678TrpTyr: 0.678 ± 0.215
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.598TyrAla: 2.598 ± 0.406
0.339TyrCys: 0.339 ± 0.131
1.864TyrAsp: 1.864 ± 0.372
1.694TyrGlu: 1.694 ± 0.333
0.734TyrPhe: 0.734 ± 0.191
2.429TyrGly: 2.429 ± 0.374
0.508TyrHis: 0.508 ± 0.166
1.017TyrIle: 1.017 ± 0.238
0.734TyrLys: 0.734 ± 0.225
2.146TyrLeu: 2.146 ± 0.371
0.282TyrMet: 0.282 ± 0.118
0.734TyrAsn: 0.734 ± 0.232
1.299TyrPro: 1.299 ± 0.253
0.791TyrGln: 0.791 ± 0.205
1.864TyrArg: 1.864 ± 0.399
1.073TyrSer: 1.073 ± 0.213
1.694TyrThr: 1.694 ± 0.354
2.542TyrVal: 2.542 ± 0.299
0.621TyrTrp: 0.621 ± 0.167
0.734TyrTyr: 0.734 ± 0.184
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 105 proteins (17706 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski