Gordonia phage DobbysSock
Average proteome isoelectric point is 5.94
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 73 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5P8DCL1|A0A5P8DCL1_9CAUD Uncharacterized protein OS=Gordonia phage DobbysSock OX=2652880 GN=51 PE=4 SV=1
MM1 pKa = 7.21 SHH3 pKa = 7.02 FAFHH7 pKa = 7.43 AEE9 pKa = 3.88 PTPVEE14 pKa = 4.31 TRR16 pKa = 11.84 HH17 pKa = 5.7 GDD19 pKa = 3.71 CYY21 pKa = 11.27 VGTGEE26 pKa = 4.44 SADD29 pKa = 3.67 GVEE32 pKa = 5.05 SIAIVTPGDD41 pKa = 3.92 DD42 pKa = 3.55 STDD45 pKa = 3.21 LHH47 pKa = 7.05 LSTDD51 pKa = 3.38 QAFVLLAEE59 pKa = 4.83 LVTAIAVLVGRR70 pKa = 11.84 EE71 pKa = 4.03 GAPLL75 pKa = 3.68
Molecular weight: 7.81 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.162
IPC2_protein 4.253
IPC_protein 4.126
Toseland 3.961
ProMoST 4.279
Dawson 4.113
Bjellqvist 4.266
Wikipedia 4.024
Rodwell 3.973
Grimsley 3.884
Solomon 4.101
Lehninger 4.05
Nozaki 4.24
DTASelect 4.406
Thurlkill 4.012
EMBOSS 4.037
Sillero 4.253
Patrickios 3.121
IPC_peptide 4.101
IPC2_peptide 4.24
IPC2.peptide.svr19 4.148
Protein with the highest isoelectric point:
>tr|A0A5P8DDH1|A0A5P8DDH1_9CAUD Uncharacterized protein OS=Gordonia phage DobbysSock OX=2652880 GN=69 PE=4 SV=1
MM1 pKa = 6.16 TTLRR5 pKa = 11.84 RR6 pKa = 11.84 CTRR9 pKa = 11.84 PGCEE13 pKa = 3.43 HH14 pKa = 7.11 RR15 pKa = 11.84 PRR17 pKa = 11.84 SRR19 pKa = 11.84 GLCHH23 pKa = 5.62 THH25 pKa = 6.78 YY26 pKa = 9.14 MDD28 pKa = 6.13 HH29 pKa = 6.62 ITRR32 pKa = 11.84 QKK34 pKa = 11.18 AYY36 pKa = 10.13 GRR38 pKa = 11.84 WEE40 pKa = 3.89 SSLIDD45 pKa = 3.68 AEE47 pKa = 4.29 PTRR50 pKa = 11.84 QHH52 pKa = 4.68 VQRR55 pKa = 11.84 LRR57 pKa = 11.84 AAGVGTRR64 pKa = 11.84 KK65 pKa = 9.59 ISEE68 pKa = 4.23 TAGVTRR74 pKa = 11.84 SVLQSLIHH82 pKa = 5.91 GKK84 pKa = 7.21 TRR86 pKa = 11.84 NGHH89 pKa = 6.0 RR90 pKa = 11.84 EE91 pKa = 4.03 PPSKK95 pKa = 9.88 TIAKK99 pKa = 9.91 RR100 pKa = 11.84 IADD103 pKa = 4.63 KK104 pKa = 10.77 ILAVPVPAQPLHH116 pKa = 6.35 NGDD119 pKa = 3.52 VPALGTRR126 pKa = 11.84 RR127 pKa = 11.84 RR128 pKa = 11.84 LQALIAHH135 pKa = 7.62 GYY137 pKa = 7.86 SQAEE141 pKa = 3.93 INRR144 pKa = 11.84 RR145 pKa = 11.84 FGLAADD151 pKa = 4.22 NLTEE155 pKa = 5.09 LIRR158 pKa = 11.84 GEE160 pKa = 4.17 RR161 pKa = 11.84 NYY163 pKa = 10.53 VDD165 pKa = 4.0 PNTEE169 pKa = 3.69 RR170 pKa = 11.84 RR171 pKa = 11.84 VTALFRR177 pKa = 11.84 EE178 pKa = 4.6 LQLTPGPCTRR188 pKa = 11.84 ARR190 pKa = 11.84 NRR192 pKa = 11.84 GKK194 pKa = 10.85 KK195 pKa = 9.9 NGWPLPLDD203 pKa = 3.41 WDD205 pKa = 4.19 EE206 pKa = 5.62 DD207 pKa = 4.45 HH208 pKa = 7.42 IDD210 pKa = 3.91 DD211 pKa = 5.31 PNYY214 pKa = 9.03 QAEE217 pKa = 4.18 RR218 pKa = 11.84 SHH220 pKa = 5.94 VRR222 pKa = 11.84 SIVRR226 pKa = 11.84 DD227 pKa = 3.49 PEE229 pKa = 4.53 RR230 pKa = 11.84 IEE232 pKa = 3.58 WRR234 pKa = 11.84 KK235 pKa = 9.75 QKK237 pKa = 10.48 VAEE240 pKa = 4.18 LTALGYY246 pKa = 9.82 SAEE249 pKa = 4.89 DD250 pKa = 2.74 IAARR254 pKa = 11.84 LRR256 pKa = 11.84 CTARR260 pKa = 11.84 TVQRR264 pKa = 11.84 DD265 pKa = 3.45 RR266 pKa = 11.84 SALGIVEE273 pKa = 4.47 RR274 pKa = 11.84 EE275 pKa = 4.06 QVASS279 pKa = 3.61
Molecular weight: 31.72 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.35
IPC2_protein 9.458
IPC_protein 10.321
Toseland 10.526
ProMoST 10.262
Dawson 10.628
Bjellqvist 10.379
Wikipedia 10.862
Rodwell 10.716
Grimsley 10.687
Solomon 10.76
Lehninger 10.716
Nozaki 10.526
DTASelect 10.365
Thurlkill 10.526
EMBOSS 10.935
Sillero 10.57
Patrickios 10.409
IPC_peptide 10.76
IPC2_peptide 9.545
IPC2.peptide.svr19 8.618
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
73
0
73
15443
38
2137
211.5
23.07
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.258 ± 0.639
0.848 ± 0.173
7.032 ± 0.424
5.964 ± 0.331
2.428 ± 0.171
8.049 ± 0.472
2.156 ± 0.226
4.462 ± 0.206
3.212 ± 0.242
7.887 ± 0.216
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.163 ± 0.149
2.927 ± 0.207
5.29 ± 0.226
3.762 ± 0.358
7.563 ± 0.419
5.329 ± 0.212
7.285 ± 0.285
7.188 ± 0.226
2.04 ± 0.163
2.156 ± 0.131
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here