Sitophilus oryzae (Rice weevil) (Curculio oryzae)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Panarthropoda; Arthropoda; Mandibulata; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Endopterygota; Coleoptera; Polyphaga; Cucujiformia; Curculionoidea; Curculionidae; Dryophthorinae;

Average proteome isoelectric point is 6.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 20351 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6J2YXW3|A0A6J2YXW3_SITOR uncharacterized protein LOC115891686 OS=Sitophilus oryzae OX=7048 GN=LOC115891686 PE=4 SV=1
MM1 pKa = 7.42KK2 pKa = 10.47SSLALLVVVYY12 pKa = 10.35LMHH15 pKa = 6.51TSMVVLEE22 pKa = 4.53VKK24 pKa = 10.15SAPFGFGLLCDD35 pKa = 4.75DD36 pKa = 5.7YY37 pKa = 11.94EE38 pKa = 6.21DD39 pKa = 4.49LLCDD43 pKa = 4.61FFDD46 pKa = 3.71MCEE49 pKa = 4.21DD50 pKa = 4.57DD51 pKa = 4.94SDD53 pKa = 4.09EE54 pKa = 4.47TTTPPPSPTTSTEE67 pKa = 3.85KK68 pKa = 10.37PSPSTQSSPITTITTTVNPITTTTVAPTTSVAPTTTVAPTPP109 pKa = 3.84

Molecular weight: 11.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6J2XRT1|A0A6J2XRT1_SITOR putative inorganic phosphate cotransporter OS=Sitophilus oryzae OX=7048 GN=LOC115880953 PE=4 SV=1
MM1 pKa = 7.41GRR3 pKa = 11.84ICIRR7 pKa = 11.84KK8 pKa = 8.04ATSTHH13 pKa = 5.08SQAGRR18 pKa = 11.84GNIFLFFLLVCPASNFEE35 pKa = 3.92GRR37 pKa = 11.84KK38 pKa = 9.07CRR40 pKa = 11.84EE41 pKa = 3.85EE42 pKa = 4.16ASITRR47 pKa = 11.84PNLNLLKK54 pKa = 10.49DD55 pKa = 3.94NPGSQRR61 pKa = 11.84DD62 pKa = 3.75FKK64 pKa = 10.1KK65 pKa = 10.81QEE67 pKa = 3.25WRR69 pKa = 11.84SQKK72 pKa = 10.33FYY74 pKa = 10.82IHH76 pKa = 6.92AAYY79 pKa = 9.9DD80 pKa = 3.66QSQKK84 pKa = 11.04NSDD87 pKa = 3.81CSRR90 pKa = 11.84HH91 pKa = 5.21RR92 pKa = 11.84RR93 pKa = 11.84IPGKK97 pKa = 9.96RR98 pKa = 11.84VYY100 pKa = 10.02CQLYY104 pKa = 10.48RR105 pKa = 11.84IDD107 pKa = 4.06RR108 pKa = 11.84NPWSWGHH115 pKa = 5.4SMCPIAVRR123 pKa = 11.84RR124 pKa = 11.84HH125 pKa = 4.92CFFRR129 pKa = 11.84RR130 pKa = 11.84IIIMVGLKK138 pKa = 9.79MNLSKK143 pKa = 10.45MRR145 pKa = 11.84WW146 pKa = 3.22

Molecular weight: 17.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

14660

5691

20351

12485201

65

25382

613.5

69.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.526 ± 0.02

1.917 ± 0.02

5.544 ± 0.013

7.149 ± 0.043

3.758 ± 0.015

5.229 ± 0.024

2.379 ± 0.011

5.981 ± 0.017

7.365 ± 0.036

8.872 ± 0.029

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.039 ± 0.01

5.538 ± 0.021

5.185 ± 0.025

4.379 ± 0.016

4.922 ± 0.017

8.277 ± 0.031

5.813 ± 0.018

5.915 ± 0.019

0.979 ± 0.006

3.234 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski