Ruegeria sp. (strain TM1040) (Silicibacter sp.)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Roseobacteraceae; Ruegeria; unclassified Ruegeria

Average proteome isoelectric point is 6.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3849 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q1GM70|Q1GM70_RUEST Thiolase OS=Ruegeria sp. (strain TM1040) OX=292414 GN=TM1040_3272 PE=3 SV=1
MM1 pKa = 8.28IEE3 pKa = 4.28FLCEE7 pKa = 3.55CVVRR11 pKa = 11.84DD12 pKa = 4.44KK13 pKa = 10.92RR14 pKa = 11.84CAMTPPVSGYY24 pKa = 8.28KK25 pKa = 9.2TGRR28 pKa = 11.84GPIVKK33 pKa = 9.6RR34 pKa = 11.84LSGVFFFIMVSLFVAPQAAQASFTSCPANTSGGDD68 pKa = 3.82PLTMNLTVDD77 pKa = 3.45EE78 pKa = 4.78CTLQSGAAVSALSDD92 pKa = 4.1DD93 pKa = 5.51LIQIQIAGGSSTNFFIFDD111 pKa = 4.14PSANDD116 pKa = 3.31LRR118 pKa = 11.84DD119 pKa = 4.04LIFSVNNGSPQGTLDD134 pKa = 3.7ADD136 pKa = 3.94GGSLSGIDD144 pKa = 3.67CAAGCTVSGTHH155 pKa = 6.28GGSPFTFTYY164 pKa = 8.54TASGGGGSLSSPAEE178 pKa = 3.74IDD180 pKa = 3.95DD181 pKa = 4.39LVSSTGNTVADD192 pKa = 4.31GGTDD196 pKa = 4.05TIAAAIDD203 pKa = 3.6SGTPTTITYY212 pKa = 9.97DD213 pKa = 3.45VSNSGSGTLSLSGLTQGTPTNASFGATSIDD243 pKa = 3.61AASVASGGTEE253 pKa = 3.49TDD255 pKa = 3.85AISITFTPSSTTDD268 pKa = 3.16GASFSVPFSFTTNDD282 pKa = 3.39ADD284 pKa = 5.03DD285 pKa = 4.66GTDD288 pKa = 2.94EE289 pKa = 4.31TTFNVTISGTINAEE303 pKa = 3.96AEE305 pKa = 4.18ISDD308 pKa = 4.13LTSSTTNTVADD319 pKa = 4.6GGSDD323 pKa = 3.41AQGTVASGSAQALIYY338 pKa = 10.11TITNSGTADD347 pKa = 3.63LSVATASSSSASNVTVNSIGAPGSTTVSAGGGTTQFTVQYY387 pKa = 8.78TPSAAGAFSFDD398 pKa = 3.41LSFVNSDD405 pKa = 3.2ADD407 pKa = 3.51EE408 pKa = 4.98DD409 pKa = 4.29PFNFTVSGTATAAPGFSQAIAPGTILADD437 pKa = 3.5GTATVTFTIDD447 pKa = 2.78NSANATAATSLDD459 pKa = 3.75FSNSLPAGVEE469 pKa = 4.17VAATPNASTTCTGGTLTAVAGSGTLSYY496 pKa = 10.71TGGTVGAGASCTVQADD512 pKa = 3.68VTAGTDD518 pKa = 3.41GSYY521 pKa = 11.35SNTSGDD527 pKa = 3.75LTSSLGNSGSASASLTVASPEE548 pKa = 3.83IDD550 pKa = 3.57LQRR553 pKa = 11.84PAATSLADD561 pKa = 4.06GDD563 pKa = 3.9TDD565 pKa = 3.52AQGNVAVGVQQVLTYY580 pKa = 9.89TVEE583 pKa = 4.08NTGNATLTLSGTPSSSAASNVSVDD607 pKa = 4.28SISAPGSSSLAASASTTFTVAYY629 pKa = 8.05TPTAAGAFSFEE640 pKa = 5.13LDD642 pKa = 3.71VSSDD646 pKa = 3.62DD647 pKa = 4.37ADD649 pKa = 3.48EE650 pKa = 4.38GTYY653 pKa = 11.13DD654 pKa = 3.64LTVSGTATAAPGFSQAIAPGTILADD679 pKa = 3.5GTATVTFTIDD689 pKa = 2.78NSANATAATSLDD701 pKa = 3.75FSNSLPAGVEE711 pKa = 4.17VAATPNAATSCTGGTLTAVAGSGTLSYY738 pKa = 10.71TGGTVGAGASCTVQADD754 pKa = 3.68VTAGTDD760 pKa = 3.41GSYY763 pKa = 11.35SNTSGDD769 pKa = 3.75LTSSLGNSGSASASLTVASPEE790 pKa = 3.83IDD792 pKa = 3.57LQRR795 pKa = 11.84PAATSLADD803 pKa = 4.06GDD805 pKa = 3.9TDD807 pKa = 3.52AQGNVAVGVQQVLTYY822 pKa = 9.89TVEE825 pKa = 4.08NTGNATLTLSGTPSSAAASNVSVDD849 pKa = 4.28SISAPGSSSLAASASTTFTVAYY871 pKa = 8.05TPTAAGAFSFEE882 pKa = 5.13LDD884 pKa = 3.71VSSDD888 pKa = 3.62DD889 pKa = 4.37ADD891 pKa = 3.48EE892 pKa = 4.38GTYY895 pKa = 11.13DD896 pKa = 3.64LTVSGTATAAPGFSQAIAPGTILADD921 pKa = 3.5GTATVTFTIDD931 pKa = 2.78NSANATAATSLDD943 pKa = 3.75FSNSLPAGVEE953 pKa = 4.17VAATPNAATSCTGGTLTAVAGSGTLSYY980 pKa = 10.71TGGTVGAGASCTVQADD996 pKa = 3.68VTAGTDD1002 pKa = 3.41GSYY1005 pKa = 11.35SNTSGDD1011 pKa = 3.75LTSSLGNSGSASASLTVASPEE1032 pKa = 3.83IDD1034 pKa = 3.57LQRR1037 pKa = 11.84PAATSLADD1045 pKa = 4.06GDD1047 pKa = 3.9TDD1049 pKa = 3.52AQGNVAVGVQQVLTYY1064 pKa = 9.6TVEE1067 pKa = 4.15NTGTATLTLSGTPSSAAASNVSVDD1091 pKa = 4.28SISAPGSSSLAASASTTFTVAYY1113 pKa = 8.05TPTAAGAFSFEE1124 pKa = 5.13LDD1126 pKa = 3.71VSSDD1130 pKa = 3.62DD1131 pKa = 4.37ADD1133 pKa = 3.48EE1134 pKa = 4.38GTYY1137 pKa = 11.13DD1138 pKa = 3.64LTVSGTADD1146 pKa = 3.34GSAEE1150 pKa = 4.0IGVGSATGGSLEE1162 pKa = 5.79DD1163 pKa = 4.69GDD1165 pKa = 4.71TDD1167 pKa = 4.78TIPGTPSAGSPTVIIYY1183 pKa = 7.5TFTNSGSGTLNITTPTVAGNISNEE1207 pKa = 4.13TNVVVNSLTLASNTVAGGGGTTTLEE1232 pKa = 3.54ISYY1235 pKa = 8.8TPQVSGAYY1243 pKa = 10.05RR1244 pKa = 11.84FDD1246 pKa = 3.66LQVANDD1252 pKa = 4.4DD1253 pKa = 3.9ADD1255 pKa = 3.46EE1256 pKa = 4.33SLFNITVAGSASGLPEE1272 pKa = 4.42IEE1274 pKa = 4.43VSSSASGALSDD1285 pKa = 5.17GDD1287 pKa = 3.73TDD1289 pKa = 5.08SIATVATPGVTASVTYY1305 pKa = 9.93TITNSGTDD1313 pKa = 3.74VLTLDD1318 pKa = 4.26APSVASNIRR1327 pKa = 11.84NQSNVTVDD1335 pKa = 3.68RR1336 pKa = 11.84LSLASTTVASGGGTTTLRR1354 pKa = 11.84VDD1356 pKa = 3.53YY1357 pKa = 8.79TATAGGAYY1365 pKa = 10.11GFDD1368 pKa = 3.94LSLGSDD1374 pKa = 4.38DD1375 pKa = 5.62ADD1377 pKa = 3.5EE1378 pKa = 5.14DD1379 pKa = 3.96PFDD1382 pKa = 3.77IVVSGTAQSVASALAAQSGSGQVSEE1407 pKa = 5.29VGAQFPAPLVVLVTDD1422 pKa = 3.64STGTGVAGVDD1432 pKa = 3.36VTFAAPASGASVVFASTGTHH1452 pKa = 5.82TEE1454 pKa = 4.15TVTTGSDD1461 pKa = 3.31GTASTSAMTANGTPSSYY1478 pKa = 11.34AGGSTLQSYY1487 pKa = 10.87DD1488 pKa = 3.22VTASATGLASVTFALTNDD1506 pKa = 3.81RR1507 pKa = 11.84DD1508 pKa = 3.95SEE1510 pKa = 4.18ADD1512 pKa = 3.08IQKK1515 pKa = 7.76TQEE1518 pKa = 3.77VIAAFVSNRR1527 pKa = 11.84ANAIVSNQPDD1537 pKa = 3.74LVSRR1541 pKa = 11.84LRR1543 pKa = 11.84QGALAGQQGRR1553 pKa = 11.84NGFDD1557 pKa = 3.13LRR1559 pKa = 11.84ATSGGRR1565 pKa = 11.84SANFSFSLRR1574 pKa = 11.84ALLEE1578 pKa = 3.74EE1579 pKa = 3.91ARR1581 pKa = 11.84RR1582 pKa = 11.84RR1583 pKa = 11.84NDD1585 pKa = 3.03GARR1588 pKa = 11.84WRR1590 pKa = 11.84EE1591 pKa = 3.99TLRR1594 pKa = 11.84HH1595 pKa = 6.08ADD1597 pKa = 3.27ASAGPGADD1605 pKa = 3.25SAADD1609 pKa = 3.42RR1610 pKa = 11.84AMASQAYY1617 pKa = 9.3ALRR1620 pKa = 11.84FRR1622 pKa = 11.84GTDD1625 pKa = 3.38PAQAAPVVTPVISSGWDD1642 pKa = 3.39FWAEE1646 pKa = 3.4GTYY1649 pKa = 10.55AITEE1653 pKa = 4.2SGNFEE1658 pKa = 4.15SRR1660 pKa = 11.84SGLFFAGADD1669 pKa = 3.93YY1670 pKa = 10.56RR1671 pKa = 11.84WSDD1674 pKa = 3.63QVLLGVMGQLDD1685 pKa = 3.72IADD1688 pKa = 4.48EE1689 pKa = 4.76DD1690 pKa = 3.89NVAAGTSASGTGWMVGPYY1708 pKa = 9.93VVVRR1712 pKa = 11.84LDD1714 pKa = 3.18QNLYY1718 pKa = 10.34LDD1720 pKa = 3.63VAATYY1725 pKa = 10.14GRR1727 pKa = 11.84SSNTVNALGLFEE1739 pKa = 6.72DD1740 pKa = 5.12DD1741 pKa = 4.4FDD1743 pKa = 4.21TEE1745 pKa = 4.43RR1746 pKa = 11.84FLLQGGLTGDD1756 pKa = 4.25FKK1758 pKa = 11.72LNARR1762 pKa = 11.84TTISPFARR1770 pKa = 11.84LTYY1773 pKa = 10.12YY1774 pKa = 10.31YY1775 pKa = 9.6EE1776 pKa = 4.23KK1777 pKa = 10.59QEE1779 pKa = 4.49SYY1781 pKa = 10.99TDD1783 pKa = 3.27TLGRR1787 pKa = 11.84VIPSQDD1793 pKa = 3.07FEE1795 pKa = 4.64LGRR1798 pKa = 11.84FEE1800 pKa = 4.84FGPKK1804 pKa = 9.75ISWEE1808 pKa = 4.16MYY1810 pKa = 9.38LQEE1813 pKa = 4.36GTQFSPYY1820 pKa = 10.25VSFSGIYY1827 pKa = 10.21DD1828 pKa = 3.59FNKK1831 pKa = 10.39LQGATPTDD1839 pKa = 3.28ATLASSQSDD1848 pKa = 3.24LRR1850 pKa = 11.84GRR1852 pKa = 11.84LEE1854 pKa = 4.12AGATFLVPDD1863 pKa = 4.45RR1864 pKa = 11.84GIKK1867 pKa = 9.53VAVEE1871 pKa = 3.91GFYY1874 pKa = 11.15DD1875 pKa = 5.3GIGTSDD1881 pKa = 3.76FEE1883 pKa = 4.93SYY1885 pKa = 10.6GASLSVLIPFF1895 pKa = 5.19

Molecular weight: 188.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q1GJX8|ENGB_RUEST Probable GTP-binding protein EngB OS=Ruegeria sp. (strain TM1040) OX=292414 GN=engB PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 10.31QPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37AGRR28 pKa = 11.84KK29 pKa = 8.54ILNARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.07SLSAA44 pKa = 3.93

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3849

0

3849

1216663

41

2150

316.1

34.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.047 ± 0.053

0.922 ± 0.013

5.95 ± 0.037

6.295 ± 0.039

3.706 ± 0.024

8.37 ± 0.042

2.092 ± 0.021

5.091 ± 0.025

3.311 ± 0.031

10.108 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.788 ± 0.023

2.657 ± 0.024

4.897 ± 0.03

3.477 ± 0.022

6.512 ± 0.043

5.536 ± 0.034

5.467 ± 0.034

7.14 ± 0.033

1.365 ± 0.017

2.27 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski