Torque teno tupaia virus (isolate Tbc-TTV14)
Average proteome isoelectric point is 6.55
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|Q91PQ3|ORF3_TTVD1 Uncharacterized ORF3 protein OS=Torque teno tupaia virus (isolate Tbc-TTV14) OX=766185 GN=ORF3 PE=4 SV=1
MM1 pKa = 8.05 DD2 pKa = 5.25 PAKK5 pKa = 10.17 IWWHH9 pKa = 5.7 SCLLSHH15 pKa = 7.52 KK16 pKa = 9.42 SWCNCTEE23 pKa = 4.0 PRR25 pKa = 11.84 NHH27 pKa = 6.6 LPGWPTSEE35 pKa = 4.1 GTSTEE40 pKa = 4.21 DD41 pKa = 3.89 GDD43 pKa = 4.53 IITDD47 pKa = 3.68 AEE49 pKa = 4.36 MLTLAEE55 pKa = 4.35 DD56 pKa = 4.12 TEE58 pKa = 4.79 GG59 pKa = 3.74
Molecular weight: 6.62 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.258
IPC2_protein 4.393
IPC_protein 4.24
Toseland 4.088
ProMoST 4.38
Dawson 4.215
Bjellqvist 4.368
Wikipedia 4.113
Rodwell 4.088
Grimsley 4.012
Solomon 4.202
Lehninger 4.151
Nozaki 4.329
DTASelect 4.482
Thurlkill 4.126
EMBOSS 4.126
Sillero 4.368
Patrickios 3.16
IPC_peptide 4.202
IPC2_peptide 4.355
IPC2.peptide.svr19 4.256
Protein with the highest isoelectric point:
>sp|Q91PQ2|ORF2_TTVD1 Uncharacterized ORF2 protein OS=Torque teno tupaia virus (isolate Tbc-TTV14) OX=766185 GN=ORF2 PE=4 SV=1
MM1 pKa = 7.8 AYY3 pKa = 10.17 FRR5 pKa = 11.84 RR6 pKa = 11.84 NFYY9 pKa = 10.19 GGRR12 pKa = 11.84 RR13 pKa = 11.84 HH14 pKa = 5.81 YY15 pKa = 10.39 YY16 pKa = 9.29 RR17 pKa = 11.84 RR18 pKa = 11.84 RR19 pKa = 11.84 NAYY22 pKa = 5.75 TRR24 pKa = 11.84 RR25 pKa = 11.84 RR26 pKa = 11.84 YY27 pKa = 8.99 RR28 pKa = 11.84 RR29 pKa = 11.84 VRR31 pKa = 11.84 RR32 pKa = 11.84 RR33 pKa = 11.84 HH34 pKa = 5.42 RR35 pKa = 11.84 GRR37 pKa = 11.84 RR38 pKa = 11.84 FPYY41 pKa = 9.3 FKK43 pKa = 9.85 QGKK46 pKa = 8.15 RR47 pKa = 11.84 RR48 pKa = 11.84 RR49 pKa = 11.84 TRR51 pKa = 11.84 LLHH54 pKa = 5.08 VTDD57 pKa = 4.27 PRR59 pKa = 11.84 YY60 pKa = 9.69 KK61 pKa = 10.0 AHH63 pKa = 6.11 CTITGWVPIAMTRR76 pKa = 11.84 IQLISQPFTVDD87 pKa = 2.83 TLSPFHH93 pKa = 6.91 VYY95 pKa = 10.08 PGGYY99 pKa = 9.63 GYY101 pKa = 11.39 GVFTLEE107 pKa = 4.6 AMYY110 pKa = 10.43 KK111 pKa = 8.03 EE112 pKa = 4.4 HH113 pKa = 7.0 LMKK116 pKa = 10.58 RR117 pKa = 11.84 NRR119 pKa = 11.84 WSRR122 pKa = 11.84 SNAGFDD128 pKa = 3.15 LGRR131 pKa = 11.84 YY132 pKa = 8.78 LGTWLTLVPHH142 pKa = 7.24 PYY144 pKa = 10.03 FSYY147 pKa = 10.79 LFYY150 pKa = 11.04 YY151 pKa = 10.38 DD152 pKa = 4.16 PEE154 pKa = 4.62 YY155 pKa = 11.47 GNTFEE160 pKa = 4.46 FKK162 pKa = 10.74 KK163 pKa = 10.37 LIHH166 pKa = 5.88 PAIMITHH173 pKa = 7.66 PKK175 pKa = 8.94 TILVLSNKK183 pKa = 10.03 RR184 pKa = 11.84 GGNRR188 pKa = 11.84 RR189 pKa = 11.84 KK190 pKa = 9.1 WPRR193 pKa = 11.84 MWIPRR198 pKa = 11.84 PAIFADD204 pKa = 3.34 GWEE207 pKa = 4.24 FQKK210 pKa = 10.85 DD211 pKa = 3.66 LCQHH215 pKa = 5.86 GLFAFGWAWVDD226 pKa = 4.76 LDD228 pKa = 4.67 RR229 pKa = 11.84 PWMADD234 pKa = 2.53 ISKK237 pKa = 9.23 PAQTIDD243 pKa = 3.06 PSTYY247 pKa = 10.48 DD248 pKa = 4.11 KK249 pKa = 11.33 KK250 pKa = 11.1 NDD252 pKa = 3.96 MINKK256 pKa = 8.11 VPNMWWKK263 pKa = 10.13 EE264 pKa = 3.8 AANDD268 pKa = 3.64 WLTEE272 pKa = 3.7 WGRR275 pKa = 11.84 PQGSGVTQQFNQLAMAGPFVLKK297 pKa = 9.68 TNKK300 pKa = 9.64 SEE302 pKa = 4.21 YY303 pKa = 11.02 VNTEE307 pKa = 3.8 LLQIILFYY315 pKa = 10.58 KK316 pKa = 10.16 SHH318 pKa = 5.83 FQWGGEE324 pKa = 3.89 FLQDD328 pKa = 4.64 KK329 pKa = 10.45 VLADD333 pKa = 3.65 PTKK336 pKa = 10.45 IPPAQTAYY344 pKa = 10.07 YY345 pKa = 8.95 QQAGQTNLYY354 pKa = 9.06 GSPYY358 pKa = 9.79 KK359 pKa = 10.11 PLSSGLHH366 pKa = 6.19 DD367 pKa = 5.63 SISEE371 pKa = 4.11 LPIASPPQNPHH382 pKa = 6.79 KK383 pKa = 10.01 YY384 pKa = 7.14 TVRR387 pKa = 11.84 PSDD390 pKa = 3.54 YY391 pKa = 10.72 DD392 pKa = 3.21 KK393 pKa = 11.7 DD394 pKa = 4.3 GILTEE399 pKa = 4.06 EE400 pKa = 4.24 SFRR403 pKa = 11.84 RR404 pKa = 11.84 LTDD407 pKa = 3.09 SPYY410 pKa = 10.32 TSDD413 pKa = 3.57 GGHH416 pKa = 6.03 SFSSFSTASDD426 pKa = 3.63 PLEE429 pKa = 4.31 GTSRR433 pKa = 11.84 YY434 pKa = 9.61 ARR436 pKa = 11.84 PLGRR440 pKa = 11.84 KK441 pKa = 4.56 TTRR444 pKa = 11.84 EE445 pKa = 3.31 PSRR448 pKa = 11.84 RR449 pKa = 11.84 RR450 pKa = 11.84 KK451 pKa = 9.21 RR452 pKa = 11.84 RR453 pKa = 11.84 RR454 pKa = 11.84 RR455 pKa = 11.84 SPSPEE460 pKa = 4.09 DD461 pKa = 3.91 EE462 pKa = 4.22 EE463 pKa = 4.47 TAPIPPKK470 pKa = 10.75 SNSEE474 pKa = 3.9 PSISGPGRR482 pKa = 11.84 ATRR485 pKa = 11.84 KK486 pKa = 9.44 QLLQRR491 pKa = 11.84 LFRR494 pKa = 11.84 RR495 pKa = 11.84 LLTSSPQQ502 pKa = 3.05
Molecular weight: 58.83 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.295
IPC2_protein 9.794
IPC_protein 10.438
Toseland 10.306
ProMoST 10.087
Dawson 10.526
Bjellqvist 10.233
Wikipedia 10.73
Rodwell 10.774
Grimsley 10.599
Solomon 10.555
Lehninger 10.511
Nozaki 10.292
DTASelect 10.233
Thurlkill 10.365
EMBOSS 10.716
Sillero 10.438
Patrickios 10.292
IPC_peptide 10.555
IPC2_peptide 9.077
IPC2.peptide.svr19 8.64
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
775
59
502
258.3
29.85
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.935 ± 0.852
1.161 ± 0.908
5.677 ± 1.101
4.774 ± 1.211
3.613 ± 1.378
5.806 ± 1.079
3.484 ± 0.694
3.871 ± 0.454
5.032 ± 0.558
8.0 ± 0.429
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.194 ± 0.258
3.226 ± 0.18
8.387 ± 0.313
3.871 ± 0.708
9.161 ± 1.825
8.0 ± 0.84
8.258 ± 1.488
2.323 ± 0.698
2.968 ± 0.692
4.258 ± 1.54
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here