Amino acid dipepetide frequency for Mycobacterium phage HufflyPuff

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.14AlaAla: 12.14 ± 1.295
1.168AlaCys: 1.168 ± 0.284
5.799AlaAsp: 5.799 ± 0.53
8.803AlaGlu: 8.803 ± 0.715
2.795AlaPhe: 2.795 ± 0.381
8.469AlaGly: 8.469 ± 0.7
2.545AlaHis: 2.545 ± 0.39
3.546AlaIle: 3.546 ± 0.418
5.006AlaLys: 5.006 ± 0.49
7.801AlaLeu: 7.801 ± 0.588
3.045AlaMet: 3.045 ± 0.408
3.045AlaAsn: 3.045 ± 0.513
4.631AlaPro: 4.631 ± 0.368
3.212AlaGln: 3.212 ± 0.355
5.882AlaArg: 5.882 ± 0.697
5.715AlaSer: 5.715 ± 0.544
6.383AlaThr: 6.383 ± 0.47
5.882AlaVal: 5.882 ± 0.42
1.836AlaTrp: 1.836 ± 0.287
2.753AlaTyr: 2.753 ± 0.357
0.0AlaXaa: 0.0 ± 0.0
Cys
1.085CysAla: 1.085 ± 0.238
0.167CysCys: 0.167 ± 0.085
0.584CysAsp: 0.584 ± 0.204
0.668CysGlu: 0.668 ± 0.196
0.292CysPhe: 0.292 ± 0.131
1.961CysGly: 1.961 ± 0.405
0.375CysHis: 0.375 ± 0.114
0.501CysIle: 0.501 ± 0.137
0.459CysLys: 0.459 ± 0.135
1.377CysLeu: 1.377 ± 0.254
0.125CysMet: 0.125 ± 0.06
0.501CysAsn: 0.501 ± 0.166
0.751CysPro: 0.751 ± 0.19
0.501CysGln: 0.501 ± 0.165
0.668CysArg: 0.668 ± 0.225
0.96CysSer: 0.96 ± 0.227
0.501CysThr: 0.501 ± 0.147
0.626CysVal: 0.626 ± 0.174
0.167CysTrp: 0.167 ± 0.104
0.459CysTyr: 0.459 ± 0.131
0.0CysXaa: 0.0 ± 0.0
Asp
5.674AspAla: 5.674 ± 0.546
1.418AspCys: 1.418 ± 0.278
4.088AspAsp: 4.088 ± 0.488
4.756AspGlu: 4.756 ± 0.53
1.46AspPhe: 1.46 ± 0.243
6.967AspGly: 6.967 ± 0.682
1.627AspHis: 1.627 ± 0.313
3.254AspIle: 3.254 ± 0.368
2.461AspLys: 2.461 ± 0.356
4.673AspLeu: 4.673 ± 0.373
1.669AspMet: 1.669 ± 0.294
1.752AspAsn: 1.752 ± 0.328
3.671AspPro: 3.671 ± 0.35
1.627AspGln: 1.627 ± 0.376
4.214AspArg: 4.214 ± 0.428
3.296AspSer: 3.296 ± 0.392
3.504AspThr: 3.504 ± 0.411
4.339AspVal: 4.339 ± 0.373
1.585AspTrp: 1.585 ± 0.225
2.753AspTyr: 2.753 ± 0.423
0.0AspXaa: 0.0 ± 0.0
Glu
6.967GluAla: 6.967 ± 0.671
1.043GluCys: 1.043 ± 0.232
4.881GluAsp: 4.881 ± 0.534
5.09GluGlu: 5.09 ± 0.581
2.545GluPhe: 2.545 ± 0.322
4.673GluGly: 4.673 ± 0.406
1.335GluHis: 1.335 ± 0.214
2.92GluIle: 2.92 ± 0.362
2.295GluLys: 2.295 ± 0.34
7.468GluLeu: 7.468 ± 0.609
1.168GluMet: 1.168 ± 0.235
2.211GluAsn: 2.211 ± 0.294
3.004GluPro: 3.004 ± 0.44
2.378GluGln: 2.378 ± 0.261
4.38GluArg: 4.38 ± 0.428
2.837GluSer: 2.837 ± 0.316
3.838GluThr: 3.838 ± 0.392
4.506GluVal: 4.506 ± 0.451
1.335GluTrp: 1.335 ± 0.227
2.545GluTyr: 2.545 ± 0.356
0.0GluXaa: 0.0 ± 0.0
Phe
2.879PheAla: 2.879 ± 0.343
0.542PheCys: 0.542 ± 0.185
2.545PheAsp: 2.545 ± 0.336
1.919PheGlu: 1.919 ± 0.22
0.876PhePhe: 0.876 ± 0.198
2.587PheGly: 2.587 ± 0.361
0.876PheHis: 0.876 ± 0.195
1.335PheIle: 1.335 ± 0.266
1.21PheLys: 1.21 ± 0.216
1.752PheLeu: 1.752 ± 0.305
0.626PheMet: 0.626 ± 0.133
1.085PheAsn: 1.085 ± 0.253
1.293PhePro: 1.293 ± 0.231
0.834PheGln: 0.834 ± 0.186
1.252PheArg: 1.252 ± 0.201
1.961PheSer: 1.961 ± 0.29
2.128PheThr: 2.128 ± 0.351
2.044PheVal: 2.044 ± 0.367
0.292PheTrp: 0.292 ± 0.103
0.96PheTyr: 0.96 ± 0.207
0.0PheXaa: 0.0 ± 0.0
Gly
7.635GlyAla: 7.635 ± 0.72
0.793GlyCys: 0.793 ± 0.2
5.715GlyAsp: 5.715 ± 0.43
5.841GlyGlu: 5.841 ± 0.483
2.128GlyPhe: 2.128 ± 0.319
9.345GlyGly: 9.345 ± 1.66
2.086GlyHis: 2.086 ± 0.282
4.088GlyIle: 4.088 ± 0.556
3.963GlyLys: 3.963 ± 0.408
6.3GlyLeu: 6.3 ± 0.379
2.086GlyMet: 2.086 ± 0.365
3.463GlyAsn: 3.463 ± 0.375
3.588GlyPro: 3.588 ± 0.467
2.545GlyGln: 2.545 ± 0.375
4.547GlyArg: 4.547 ± 0.454
5.34GlySer: 5.34 ± 0.544
6.425GlyThr: 6.425 ± 0.566
6.174GlyVal: 6.174 ± 0.543
1.752GlyTrp: 1.752 ± 0.299
3.463GlyTyr: 3.463 ± 0.42
0.0GlyXaa: 0.0 ± 0.0
His
1.877HisAla: 1.877 ± 0.321
0.542HisCys: 0.542 ± 0.137
1.669HisAsp: 1.669 ± 0.319
1.418HisGlu: 1.418 ± 0.275
0.668HisPhe: 0.668 ± 0.183
2.336HisGly: 2.336 ± 0.336
1.085HisHis: 1.085 ± 0.258
1.001HisIle: 1.001 ± 0.228
1.126HisLys: 1.126 ± 0.196
2.545HisLeu: 2.545 ± 0.355
0.501HisMet: 0.501 ± 0.13
1.001HisAsn: 1.001 ± 0.185
1.752HisPro: 1.752 ± 0.278
0.918HisGln: 0.918 ± 0.268
1.46HisArg: 1.46 ± 0.243
1.293HisSer: 1.293 ± 0.206
1.252HisThr: 1.252 ± 0.242
1.335HisVal: 1.335 ± 0.256
0.417HisTrp: 0.417 ± 0.135
1.126HisTyr: 1.126 ± 0.239
0.0HisXaa: 0.0 ± 0.0
Ile
4.756IleAla: 4.756 ± 0.5
0.417IleCys: 0.417 ± 0.147
3.379IleAsp: 3.379 ± 0.4
3.546IleGlu: 3.546 ± 0.391
1.168IlePhe: 1.168 ± 0.272
3.171IleGly: 3.171 ± 0.408
1.418IleHis: 1.418 ± 0.283
1.627IleIle: 1.627 ± 0.343
1.961IleLys: 1.961 ± 0.255
3.129IleLeu: 3.129 ± 0.451
0.96IleMet: 0.96 ± 0.185
1.752IleAsn: 1.752 ± 0.287
2.587IlePro: 2.587 ± 0.316
1.71IleGln: 1.71 ± 0.286
2.795IleArg: 2.795 ± 0.334
2.545IleSer: 2.545 ± 0.355
2.67IleThr: 2.67 ± 0.346
3.045IleVal: 3.045 ± 0.375
0.417IleTrp: 0.417 ± 0.119
1.126IleTyr: 1.126 ± 0.27
0.0IleXaa: 0.0 ± 0.0
Lys
4.673LysAla: 4.673 ± 0.44
0.751LysCys: 0.751 ± 0.183
2.587LysAsp: 2.587 ± 0.339
2.169LysGlu: 2.169 ± 0.289
1.168LysPhe: 1.168 ± 0.202
3.421LysGly: 3.421 ± 0.419
1.335LysHis: 1.335 ± 0.244
1.71LysIle: 1.71 ± 0.261
2.253LysLys: 2.253 ± 0.356
3.963LysLeu: 3.963 ± 0.416
1.126LysMet: 1.126 ± 0.224
1.585LysAsn: 1.585 ± 0.295
2.461LysPro: 2.461 ± 0.348
1.585LysGln: 1.585 ± 0.261
3.254LysArg: 3.254 ± 0.477
2.795LysSer: 2.795 ± 0.427
1.961LysThr: 1.961 ± 0.247
3.254LysVal: 3.254 ± 0.295
1.168LysTrp: 1.168 ± 0.222
1.085LysTyr: 1.085 ± 0.219
0.0LysXaa: 0.0 ± 0.0
Leu
9.095LeuAla: 9.095 ± 0.817
0.709LeuCys: 0.709 ± 0.182
4.589LeuAsp: 4.589 ± 0.471
4.714LeuGlu: 4.714 ± 0.536
2.128LeuPhe: 2.128 ± 0.313
5.924LeuGly: 5.924 ± 0.509
1.585LeuHis: 1.585 ± 0.265
3.88LeuIle: 3.88 ± 0.41
4.005LeuLys: 4.005 ± 0.403
6.884LeuLeu: 6.884 ± 0.611
2.295LeuMet: 2.295 ± 0.308
3.421LeuAsn: 3.421 ± 0.378
3.922LeuPro: 3.922 ± 0.445
2.753LeuGln: 2.753 ± 0.323
5.715LeuArg: 5.715 ± 0.512
4.839LeuSer: 4.839 ± 0.498
6.049LeuThr: 6.049 ± 0.571
5.257LeuVal: 5.257 ± 0.444
1.252LeuTrp: 1.252 ± 0.267
1.877LeuTyr: 1.877 ± 0.25
0.0LeuXaa: 0.0 ± 0.0
Met
2.336MetAla: 2.336 ± 0.35
0.292MetCys: 0.292 ± 0.112
1.418MetAsp: 1.418 ± 0.245
1.502MetGlu: 1.502 ± 0.289
0.709MetPhe: 0.709 ± 0.178
1.669MetGly: 1.669 ± 0.296
0.459MetHis: 0.459 ± 0.157
0.793MetIle: 0.793 ± 0.164
1.585MetLys: 1.585 ± 0.314
1.502MetLeu: 1.502 ± 0.223
0.417MetMet: 0.417 ± 0.147
0.834MetAsn: 0.834 ± 0.189
1.085MetPro: 1.085 ± 0.191
0.626MetGln: 0.626 ± 0.216
1.377MetArg: 1.377 ± 0.254
2.92MetSer: 2.92 ± 0.436
1.919MetThr: 1.919 ± 0.293
1.502MetVal: 1.502 ± 0.241
0.542MetTrp: 0.542 ± 0.161
0.334MetTyr: 0.334 ± 0.115
0.0MetXaa: 0.0 ± 0.0
Asn
2.712AsnAla: 2.712 ± 0.338
0.292AsnCys: 0.292 ± 0.109
2.086AsnAsp: 2.086 ± 0.291
2.003AsnGlu: 2.003 ± 0.288
1.043AsnPhe: 1.043 ± 0.276
4.547AsnGly: 4.547 ± 0.576
0.751AsnHis: 0.751 ± 0.198
1.293AsnIle: 1.293 ± 0.316
0.876AsnLys: 0.876 ± 0.162
3.463AsnLeu: 3.463 ± 0.458
0.417AsnMet: 0.417 ± 0.165
0.876AsnAsn: 0.876 ± 0.202
3.004AsnPro: 3.004 ± 0.33
1.126AsnGln: 1.126 ± 0.186
1.961AsnArg: 1.961 ± 0.284
1.836AsnSer: 1.836 ± 0.311
1.794AsnThr: 1.794 ± 0.3
2.044AsnVal: 2.044 ± 0.315
0.626AsnTrp: 0.626 ± 0.158
0.876AsnTyr: 0.876 ± 0.181
0.0AsnXaa: 0.0 ± 0.0
Pro
5.09ProAla: 5.09 ± 0.533
0.584ProCys: 0.584 ± 0.169
3.796ProAsp: 3.796 ± 0.358
3.546ProGlu: 3.546 ± 0.362
1.752ProPhe: 1.752 ± 0.285
5.09ProGly: 5.09 ± 0.63
1.544ProHis: 1.544 ± 0.298
2.545ProIle: 2.545 ± 0.316
2.628ProLys: 2.628 ± 0.483
3.671ProLeu: 3.671 ± 0.334
1.252ProMet: 1.252 ± 0.188
1.836ProAsn: 1.836 ± 0.231
3.087ProPro: 3.087 ± 0.402
1.794ProGln: 1.794 ± 0.265
2.795ProArg: 2.795 ± 0.391
2.92ProSer: 2.92 ± 0.302
3.504ProThr: 3.504 ± 0.354
3.63ProVal: 3.63 ± 0.378
1.168ProTrp: 1.168 ± 0.205
1.502ProTyr: 1.502 ± 0.196
0.0ProXaa: 0.0 ± 0.0
Gln
3.713GlnAla: 3.713 ± 0.375
0.417GlnCys: 0.417 ± 0.157
1.669GlnAsp: 1.669 ± 0.255
1.919GlnGlu: 1.919 ± 0.271
0.751GlnPhe: 0.751 ± 0.182
2.295GlnGly: 2.295 ± 0.352
0.375GlnHis: 0.375 ± 0.115
1.544GlnIle: 1.544 ± 0.266
1.544GlnLys: 1.544 ± 0.299
3.504GlnLeu: 3.504 ± 0.503
1.001GlnMet: 1.001 ± 0.241
0.834GlnAsn: 0.834 ± 0.204
1.752GlnPro: 1.752 ± 0.275
1.001GlnGln: 1.001 ± 0.245
2.128GlnArg: 2.128 ± 0.223
2.044GlnSer: 2.044 ± 0.272
1.627GlnThr: 1.627 ± 0.211
2.461GlnVal: 2.461 ± 0.37
0.876GlnTrp: 0.876 ± 0.193
0.96GlnTyr: 0.96 ± 0.194
0.0GlnXaa: 0.0 ± 0.0
Arg
6.925ArgAla: 6.925 ± 0.711
0.793ArgCys: 0.793 ± 0.226
4.422ArgAsp: 4.422 ± 0.551
3.463ArgGlu: 3.463 ± 0.403
1.919ArgPhe: 1.919 ± 0.351
4.047ArgGly: 4.047 ± 0.387
2.003ArgHis: 2.003 ± 0.336
2.461ArgIle: 2.461 ± 0.336
3.129ArgLys: 3.129 ± 0.341
4.673ArgLeu: 4.673 ± 0.519
2.086ArgMet: 2.086 ± 0.299
1.585ArgAsn: 1.585 ± 0.205
3.254ArgPro: 3.254 ± 0.374
1.919ArgGln: 1.919 ± 0.308
4.214ArgArg: 4.214 ± 0.571
2.587ArgSer: 2.587 ± 0.334
2.67ArgThr: 2.67 ± 0.35
4.255ArgVal: 4.255 ± 0.512
1.585ArgTrp: 1.585 ± 0.318
2.378ArgTyr: 2.378 ± 0.293
0.0ArgXaa: 0.0 ± 0.0
Ser
5.09SerAla: 5.09 ± 0.565
0.375SerCys: 0.375 ± 0.115
3.463SerAsp: 3.463 ± 0.4
3.504SerGlu: 3.504 ± 0.413
1.877SerPhe: 1.877 ± 0.324
6.008SerGly: 6.008 ± 0.726
1.627SerHis: 1.627 ± 0.243
2.128SerIle: 2.128 ± 0.314
2.211SerLys: 2.211 ± 0.346
4.923SerLeu: 4.923 ± 0.514
1.293SerMet: 1.293 ± 0.19
2.044SerAsn: 2.044 ± 0.276
2.92SerPro: 2.92 ± 0.325
1.502SerGln: 1.502 ± 0.327
3.463SerArg: 3.463 ± 0.48
3.504SerSer: 3.504 ± 0.512
3.713SerThr: 3.713 ± 0.496
3.838SerVal: 3.838 ± 0.391
1.627SerTrp: 1.627 ± 0.259
1.418SerTyr: 1.418 ± 0.255
0.0SerXaa: 0.0 ± 0.0
Thr
6.216ThrAla: 6.216 ± 0.538
0.668ThrCys: 0.668 ± 0.164
3.88ThrAsp: 3.88 ± 0.452
4.172ThrGlu: 4.172 ± 0.466
1.919ThrPhe: 1.919 ± 0.317
5.215ThrGly: 5.215 ± 0.499
1.168ThrHis: 1.168 ± 0.21
3.338ThrIle: 3.338 ± 0.403
2.336ThrLys: 2.336 ± 0.347
5.048ThrLeu: 5.048 ± 0.461
1.46ThrMet: 1.46 ± 0.28
2.211ThrAsn: 2.211 ± 0.311
5.006ThrPro: 5.006 ± 0.55
1.961ThrGln: 1.961 ± 0.318
2.712ThrArg: 2.712 ± 0.328
2.503ThrSer: 2.503 ± 0.297
3.171ThrThr: 3.171 ± 0.4
5.799ThrVal: 5.799 ± 0.538
1.335ThrTrp: 1.335 ± 0.279
1.919ThrTyr: 1.919 ± 0.253
0.0ThrXaa: 0.0 ± 0.0
Val
7.384ValAla: 7.384 ± 0.496
1.001ValCys: 1.001 ± 0.217
4.881ValAsp: 4.881 ± 0.52
5.59ValGlu: 5.59 ± 0.557
2.169ValPhe: 2.169 ± 0.315
4.923ValGly: 4.923 ± 0.409
1.71ValHis: 1.71 ± 0.279
3.755ValIle: 3.755 ± 0.421
3.504ValLys: 3.504 ± 0.362
4.005ValLeu: 4.005 ± 0.447
1.418ValMet: 1.418 ± 0.249
2.378ValAsn: 2.378 ± 0.316
3.338ValPro: 3.338 ± 0.388
2.336ValGln: 2.336 ± 0.366
4.297ValArg: 4.297 ± 0.487
3.755ValSer: 3.755 ± 0.378
5.215ValThr: 5.215 ± 0.717
5.757ValVal: 5.757 ± 0.675
0.793ValTrp: 0.793 ± 0.214
2.044ValTyr: 2.044 ± 0.282
0.0ValXaa: 0.0 ± 0.0
Trp
1.585TrpAla: 1.585 ± 0.258
0.334TrpCys: 0.334 ± 0.112
1.585TrpAsp: 1.585 ± 0.226
0.834TrpGlu: 0.834 ± 0.172
0.793TrpPhe: 0.793 ± 0.19
1.252TrpGly: 1.252 ± 0.285
0.668TrpHis: 0.668 ± 0.166
1.001TrpIle: 1.001 ± 0.223
0.709TrpLys: 0.709 ± 0.158
1.71TrpLeu: 1.71 ± 0.282
0.25TrpMet: 0.25 ± 0.129
0.626TrpAsn: 0.626 ± 0.162
1.001TrpPro: 1.001 ± 0.232
0.918TrpGln: 0.918 ± 0.215
0.751TrpArg: 0.751 ± 0.171
1.252TrpSer: 1.252 ± 0.234
1.168TrpThr: 1.168 ± 0.252
2.253TrpVal: 2.253 ± 0.326
0.459TrpTrp: 0.459 ± 0.153
0.668TrpTyr: 0.668 ± 0.164
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.67TyrAla: 2.67 ± 0.339
0.417TyrCys: 0.417 ± 0.16
1.752TyrAsp: 1.752 ± 0.329
1.961TyrGlu: 1.961 ± 0.25
0.834TyrPhe: 0.834 ± 0.214
3.379TyrGly: 3.379 ± 0.451
0.751TyrHis: 0.751 ± 0.189
1.46TyrIle: 1.46 ± 0.222
1.043TyrLys: 1.043 ± 0.247
2.503TyrLeu: 2.503 ± 0.279
0.584TyrMet: 0.584 ± 0.155
0.584TyrAsn: 0.584 ± 0.144
1.627TyrPro: 1.627 ± 0.314
1.168TyrGln: 1.168 ± 0.258
2.503TyrArg: 2.503 ± 0.311
1.627TyrSer: 1.627 ± 0.25
2.545TyrThr: 2.545 ± 0.339
2.253TyrVal: 2.253 ± 0.283
0.584TyrTrp: 0.584 ± 0.166
0.793TyrTyr: 0.793 ± 0.219
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 146 proteins (23971 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski