Flavobacterium gilvum

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Flavobacterium

Average proteome isoelectric point is 6.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3653 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A085ELD3|A0A085ELD3_9FLAO Uncharacterized protein OS=Flavobacterium gilvum OX=1492737 GN=FEM08_12070 PE=4 SV=1
MM1 pKa = 7.28EE2 pKa = 5.45KK3 pKa = 10.15KK4 pKa = 10.57YY5 pKa = 10.95PNLPFNLTVFSIVSSQALFKK25 pKa = 10.75RR26 pKa = 11.84ILLLMTFYY34 pKa = 11.08LCSTTIISAQCNSDD48 pKa = 3.36VPIPFIEE55 pKa = 4.45STFGGLTATSSISGVCVCTALNEE78 pKa = 4.06PRR80 pKa = 11.84LIDD83 pKa = 4.12ANLNNFATAVVAVGAIGTEE102 pKa = 4.06NLRR105 pKa = 11.84VTDD108 pKa = 3.63TNTDD112 pKa = 3.74YY113 pKa = 11.56LAGTYY118 pKa = 10.43AGFKK122 pKa = 10.29INTGGLLNVNLLGSITITTYY142 pKa = 11.15LNGVEE147 pKa = 4.37KK148 pKa = 10.73EE149 pKa = 4.5SVTGASLLGVGLLNGSSNDD168 pKa = 3.42YY169 pKa = 10.92VVGFNTKK176 pKa = 9.15QSFDD180 pKa = 4.03AIEE183 pKa = 4.04ISVGGLVSLLNTVNVYY199 pKa = 10.63YY200 pKa = 9.82PVIRR204 pKa = 11.84NYY206 pKa = 10.51CAGPALICNTATAMNLPVYY225 pKa = 9.38PVSLEE230 pKa = 3.89PSHH233 pKa = 6.45TGVSGVSIGNIYY245 pKa = 10.72DD246 pKa = 3.64GEE248 pKa = 4.32NVISSNTNDD257 pKa = 3.31FATISLTAGVLATGNISVKK276 pKa = 10.63DD277 pKa = 3.66QLTDD281 pKa = 3.7YY282 pKa = 10.98PVGTYY287 pKa = 10.65AGFEE291 pKa = 4.09IEE293 pKa = 4.65NTSLLNVSALGNVTVTTYY311 pKa = 11.41LDD313 pKa = 3.78GVAKK317 pKa = 10.19QIISGNNLVATGTLLNSSGRR337 pKa = 11.84YY338 pKa = 8.49KK339 pKa = 10.9VGFVSTTSFDD349 pKa = 3.54EE350 pKa = 4.15VQISINQTLGVNLGSTKK367 pKa = 10.31VYY369 pKa = 10.44GAVFEE374 pKa = 4.57NFCAGPDD381 pKa = 3.82LVCNTQTGMVAPTYY395 pKa = 9.76PVYY398 pKa = 10.8INAANTGIEE407 pKa = 4.24GVACLLCSVKK417 pKa = 10.56NAEE420 pKa = 4.21NLIDD424 pKa = 3.84QTPSNYY430 pKa = 10.46AEE432 pKa = 4.19INLTAGVLASGSISVKK448 pKa = 10.59DD449 pKa = 3.58QIKK452 pKa = 10.18DD453 pKa = 3.52YY454 pKa = 10.6PIGSFVGFTVEE465 pKa = 4.59NPALLSVNALDD476 pKa = 5.6AITITTYY483 pKa = 11.3LDD485 pKa = 3.57GVPQEE490 pKa = 4.12IKK492 pKa = 10.8AGNTALATVGTNLLVGTSPQTIGFIATKK520 pKa = 10.4SFDD523 pKa = 3.47EE524 pKa = 4.36ARR526 pKa = 11.84ITIANTASVNLGIVRR541 pKa = 11.84VYY543 pKa = 10.31QAVFEE548 pKa = 4.36KK549 pKa = 10.59LCPVTLEE556 pKa = 4.14CNKK559 pKa = 9.93TYY561 pKa = 11.18SLTNPTFPVIIDD573 pKa = 3.65SNKK576 pKa = 9.34TGVQGVACVSCAVNDD591 pKa = 4.07TNNVLTASTTDD602 pKa = 3.73FARR605 pKa = 11.84IMVTAGVIAPASIAVLDD622 pKa = 3.89QLSTYY627 pKa = 9.89PAGTIAGFTIRR638 pKa = 11.84DD639 pKa = 3.89RR640 pKa = 11.84NSLLQLDD647 pKa = 5.08LFNSLTISTYY657 pKa = 10.89LDD659 pKa = 3.22GVLQEE664 pKa = 4.82SRR666 pKa = 11.84SASNIINLNLLTSFGTGAGMYY687 pKa = 9.99NVGFRR692 pKa = 11.84ATKK695 pKa = 10.18SFDD698 pKa = 3.65EE699 pKa = 3.98IRR701 pKa = 11.84ITVGSLASVINDD713 pKa = 2.94INVYY717 pKa = 9.87GAFVNTSGTSGGTLFCAVIDD737 pKa = 5.21AIDD740 pKa = 4.45DD741 pKa = 3.84NFPPIAGASGDD752 pKa = 4.11SNIGNVLTNDD762 pKa = 3.78TLSGNPATISDD773 pKa = 3.92VNLAVLTPAVPLTVGASVPSIDD795 pKa = 3.2IATGVVSVPAGTPAGNYY812 pKa = 9.75SITYY816 pKa = 7.04QICYY820 pKa = 9.64KK821 pKa = 10.56LNSLTCDD828 pKa = 3.03TATVNIVVTAATINAEE844 pKa = 4.2DD845 pKa = 4.2DD846 pKa = 4.01TNTTTIYY853 pKa = 10.98GNVGGNPGINVYY865 pKa = 11.18ANDD868 pKa = 3.86TTVGGVLINPSDD880 pKa = 3.87VTLSSTPNGPLTVNADD896 pKa = 3.36GSVTVAPNTPAGTYY910 pKa = 7.77TVTYY914 pKa = 7.35TICEE918 pKa = 4.07NLNGSNCDD926 pKa = 3.46SATVTVLVSAVPIDD940 pKa = 4.25AVNDD944 pKa = 3.77NNNTAPIIYY953 pKa = 10.21GNVGGNAGINVYY965 pKa = 11.11ANDD968 pKa = 3.94TLNGVLVNPSDD979 pKa = 4.1VIFGSSPNGPLTVNADD995 pKa = 3.36GSVTVAPNTPAGTYY1009 pKa = 8.32TVDD1012 pKa = 3.25YY1013 pKa = 7.94TICEE1017 pKa = 4.35KK1018 pKa = 11.02LNSANNCDD1026 pKa = 3.63TATVTVVVSAAVVDD1040 pKa = 4.21AVVDD1044 pKa = 4.06TNTLPVYY1051 pKa = 10.89GNVGGNTDD1059 pKa = 3.15INVYY1063 pKa = 11.11ANDD1066 pKa = 3.88TLNGVLINPSDD1077 pKa = 3.74FTLISTPTSILTINPDD1093 pKa = 2.85GSVTVAPKK1101 pKa = 9.98TPAGTYY1107 pKa = 9.92SVDD1110 pKa = 3.3YY1111 pKa = 8.47TICEE1115 pKa = 4.35KK1116 pKa = 11.06LNSTNNCDD1124 pKa = 3.37TATVTVVVSKK1134 pKa = 11.02AVVDD1138 pKa = 4.01AVVDD1142 pKa = 4.06TNTLPVYY1149 pKa = 10.89GNVGGNTDD1157 pKa = 3.51INVLANDD1164 pKa = 4.06TQNGVPVNRR1173 pKa = 11.84LDD1175 pKa = 3.55VTLTSTPTSILTINPDD1191 pKa = 2.85GSVTVAPNTPAGSYY1205 pKa = 9.59TIDD1208 pKa = 3.28YY1209 pKa = 7.52TICEE1213 pKa = 4.39KK1214 pKa = 11.25LNLTTNCDD1222 pKa = 3.37TATVTVVVSAAVVDD1236 pKa = 4.21AVVDD1240 pKa = 4.06TNTLPVYY1247 pKa = 10.89GNVGGNTDD1255 pKa = 3.15INVYY1259 pKa = 11.11ANDD1262 pKa = 3.88TLNGVLINPSDD1273 pKa = 3.86FTLTSTPTSQLKK1285 pKa = 10.62VNTDD1289 pKa = 3.03GSVTVAPNTPVGTYY1303 pKa = 10.2SVDD1306 pKa = 3.32YY1307 pKa = 8.46TICEE1311 pKa = 4.2KK1312 pKa = 11.25LNLTTNCDD1320 pKa = 3.37TATVTVVVSAAVVDD1334 pKa = 4.21AVVDD1338 pKa = 4.06TNTLPVYY1345 pKa = 10.89GNVGGNTGINVLANDD1360 pKa = 4.13TQNGVPVNPLDD1371 pKa = 3.52VTLISTPTSILTINPDD1387 pKa = 2.86GSVTVASNTPVGTYY1401 pKa = 10.29SVDD1404 pKa = 3.32YY1405 pKa = 8.46TICEE1409 pKa = 4.3KK1410 pKa = 11.08LNSTTNCDD1418 pKa = 3.28TATVTVVVSAAVVDD1432 pKa = 4.21AVVDD1436 pKa = 4.06TNTLPVYY1443 pKa = 10.89GNVGGNTGINVLANDD1458 pKa = 4.13TQNGVPVNPLDD1469 pKa = 3.68VTLTSTPNGPLTINPDD1485 pKa = 2.98GSVTVAPNTPAGTYY1499 pKa = 10.05SVDD1502 pKa = 3.3YY1503 pKa = 8.47TICEE1507 pKa = 4.3KK1508 pKa = 11.08LNSTTNCDD1516 pKa = 3.27TATVTVAVSFPSIAVVKK1533 pKa = 8.59TSVVGGTGKK1542 pKa = 10.75VGDD1545 pKa = 3.97LVTYY1549 pKa = 8.32TFTVTNTGDD1558 pKa = 3.24TTLNGIVINDD1568 pKa = 3.71ALTNSVNLSVTPSTLVPNGIGTATSTYY1595 pKa = 9.96IIKK1598 pKa = 10.61QSDD1601 pKa = 2.96IDD1603 pKa = 3.62AGKK1606 pKa = 8.19ITNSATVTGTIPTGGTVTDD1625 pKa = 3.92ISGTTITDD1633 pKa = 3.22NTPTVTTLTTSPSIAVVKK1651 pKa = 9.36TSVVGGTGKK1660 pKa = 10.74VGDD1663 pKa = 3.86VVTYY1667 pKa = 9.34TFTVTNTGNTTLSNIVINDD1686 pKa = 3.31VLTNTVNKK1694 pKa = 9.79VVTVGNLAPNATATTTVTYY1713 pKa = 10.13IIKK1716 pKa = 10.54QSDD1719 pKa = 2.96IDD1721 pKa = 3.62AGKK1724 pKa = 8.19ITNSATVTGTTPAGGTVTDD1743 pKa = 4.01ISGTAITNDD1752 pKa = 3.45TPTVTTLTTSPSIAVVKK1769 pKa = 9.35TSVVGTTIKK1778 pKa = 10.95VGDD1781 pKa = 3.66KK1782 pKa = 9.05VTYY1785 pKa = 9.55TFTVTNTGNTTLNNIVINDD1804 pKa = 3.82ALTNTVNLAVTPSTLAPNSIGTATSTYY1831 pKa = 9.96IIKK1834 pKa = 10.61QSDD1837 pKa = 3.03IDD1839 pKa = 3.95AGQITNSATVIGTIPTGGTVTDD1861 pKa = 3.8ISGTAVNNDD1870 pKa = 3.04NPTITTLNTNSAISIMKK1887 pKa = 9.99EE1888 pKa = 3.37GTYY1891 pKa = 10.24EE1892 pKa = 4.16DD1893 pKa = 4.22KK1894 pKa = 11.51NKK1896 pKa = 11.07DD1897 pKa = 3.22GIANVGDD1904 pKa = 3.74VINYY1908 pKa = 9.27SFTVTNTGNVSLHH1921 pKa = 6.67NITIQDD1927 pKa = 3.75NNVTTVKK1934 pKa = 9.64GTLSSLSVGASDD1946 pKa = 4.26SVAFSAVYY1954 pKa = 10.48SITDD1958 pKa = 3.28ADD1960 pKa = 3.61IVSGYY1965 pKa = 9.87VYY1967 pKa = 10.84NSAIISALSSANAKK1981 pKa = 7.9VTAVSTDD1988 pKa = 3.46PTPCIQCPVLSNCLTCTITAIPQAPSIAIIKK2019 pKa = 10.14KK2020 pKa = 10.02GVFRR2024 pKa = 11.84DD2025 pKa = 3.9EE2026 pKa = 4.83NGDD2029 pKa = 3.43GYY2031 pKa = 11.67AEE2033 pKa = 4.11IGEE2036 pKa = 4.45TIDD2039 pKa = 3.78YY2040 pKa = 10.73SFTIMNTGNLPLTNVTLTDD2059 pKa = 3.67ALVGIVISGGPINLAVGATDD2079 pKa = 3.24TTTFKK2084 pKa = 10.43ATYY2087 pKa = 9.6HH2088 pKa = 5.09VTQQDD2093 pKa = 3.93VVKK2096 pKa = 11.09GSVTNQAIIKK2106 pKa = 9.65GFTPTGAQVTDD2117 pKa = 4.43LSDD2120 pKa = 4.74DD2121 pKa = 4.18DD2122 pKa = 6.21SPLQDD2127 pKa = 4.46DD2128 pKa = 4.19PTVLEE2133 pKa = 4.33IDD2135 pKa = 3.69GCTLEE2140 pKa = 4.29VFNAVSPDD2148 pKa = 3.3GDD2150 pKa = 3.67GLNDD2154 pKa = 3.37IFRR2157 pKa = 11.84IRR2159 pKa = 11.84GIEE2162 pKa = 4.19CYY2164 pKa = 9.52PKK2166 pKa = 10.89NSVQVFNRR2174 pKa = 11.84WGVKK2178 pKa = 8.93VYY2180 pKa = 9.92EE2181 pKa = 4.19AEE2183 pKa = 4.55GYY2185 pKa = 11.1NNDD2188 pKa = 2.95TVAFKK2193 pKa = 10.54GYY2195 pKa = 9.93SEE2197 pKa = 4.7GRR2199 pKa = 11.84VTVNKK2204 pKa = 10.55SEE2206 pKa = 3.96GLPTGTYY2213 pKa = 9.69FYY2215 pKa = 10.57VIKK2218 pKa = 11.22YY2219 pKa = 9.82EE2220 pKa = 4.21DD2221 pKa = 3.83FSGAGIDD2228 pKa = 3.28KK2229 pKa = 10.61SGYY2232 pKa = 10.68LNISRR2237 pKa = 11.84NN2238 pKa = 3.55

Molecular weight: 230.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A085EJ68|A0A085EJ68_9FLAO Peptidase M3 OS=Flavobacterium gilvum OX=1492737 GN=FEM08_18670 PE=3 SV=1
MM1 pKa = 7.58PFWRR5 pKa = 11.84SLIIDD10 pKa = 3.76IGLYY14 pKa = 8.45ICKK17 pKa = 9.7SRR19 pKa = 11.84SVIGLGFLTVNKK31 pKa = 8.61KK32 pKa = 9.23TIVSIPDD39 pKa = 3.06SRR41 pKa = 11.84YY42 pKa = 8.83CRR44 pKa = 11.84PFFVFGNRR52 pKa = 11.84IHH54 pKa = 6.93GKK56 pKa = 9.58ASTKK60 pKa = 7.62EE61 pKa = 4.11THH63 pKa = 5.77FHH65 pKa = 6.88NSTKK69 pKa = 10.38LCRR72 pKa = 11.84ISIRR76 pKa = 11.84YY77 pKa = 7.76IQDD80 pKa = 2.82RR81 pKa = 11.84RR82 pKa = 11.84HH83 pKa = 6.26FISIFSLKK91 pKa = 9.15TSRR94 pKa = 11.84RR95 pKa = 11.84KK96 pKa = 10.02INILNQIGIGKK107 pKa = 6.26TQSFLLSRR115 pKa = 11.84SDD117 pKa = 3.52EE118 pKa = 3.68KK119 pKa = 10.99RR120 pKa = 11.84AIYY123 pKa = 9.44FYY125 pKa = 10.69FVNINQILIIISTSNSILCTQLIIGIDD152 pKa = 3.55SGQSFQNVFDD162 pKa = 3.64TGIGSRR168 pKa = 11.84HH169 pKa = 5.68KK170 pKa = 10.75PNVFGFKK177 pKa = 10.61SNNPLNCFFIGRR189 pKa = 11.84INDD192 pKa = 3.72NFSHH196 pKa = 7.05LLLIITHH203 pKa = 5.0VHH205 pKa = 5.91IKK207 pKa = 10.04NKK209 pKa = 9.92NFIWRR214 pKa = 11.84QQ215 pKa = 3.11

Molecular weight: 24.92 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3653

0

3653

1237433

37

4660

338.7

38.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.42 ± 0.041

0.827 ± 0.015

5.345 ± 0.029

6.26 ± 0.051

5.267 ± 0.038

6.518 ± 0.045

1.662 ± 0.018

7.91 ± 0.043

8.237 ± 0.052

8.883 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.246 ± 0.022

6.442 ± 0.043

3.582 ± 0.027

3.364 ± 0.02

3.118 ± 0.028

6.529 ± 0.032

5.9 ± 0.064

6.253 ± 0.036

1.16 ± 0.017

4.077 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski