Coconut foliar decay virus
Average proteome isoelectric point is 7.56
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q66007|Q66007_9VIRU ORF6 OS=Coconut foliar decay virus OX=12474 PE=4 SV=1
MM1 pKa = 7.63 TGFTWSRR8 pKa = 11.84 PVVPTNRR15 pKa = 11.84 IEE17 pKa = 4.15 TTVRR21 pKa = 11.84 RR22 pKa = 11.84 NGCFSSTEE30 pKa = 3.74 SRR32 pKa = 11.84 LVLEE36 pKa = 4.97 SKK38 pKa = 10.59 GHH40 pKa = 6.5 DD41 pKa = 3.43 WPNDD45 pKa = 3.41 LLRR48 pKa = 11.84 NLMNSAWKK56 pKa = 8.24 TQADD60 pKa = 4.22 TEE62 pKa = 4.38 DD63 pKa = 3.32 ALYY66 pKa = 10.4 TEE68 pKa = 5.28 LRR70 pKa = 11.84 WNGQDD75 pKa = 3.21 GPLKK79 pKa = 10.25 IRR81 pKa = 11.84 SHH83 pKa = 5.01 FHH85 pKa = 4.53 ITIGSLKK92 pKa = 9.84 CCLRR96 pKa = 11.84 SEE98 pKa = 4.36 SQRR101 pKa = 11.84 TIAQSSGYY109 pKa = 10.45 ADD111 pKa = 3.51 EE112 pKa = 4.63 TEE114 pKa = 4.5 EE115 pKa = 4.28 TGSPCLPNISDD126 pKa = 3.85 SSPTGSTHH134 pKa = 4.56 VVEE137 pKa = 5.39 PEE139 pKa = 3.48 RR140 pKa = 11.84 TYY142 pKa = 10.44 CTSTSRR148 pKa = 11.84 TQNEE152 pKa = 3.83 II153 pKa = 3.32
Molecular weight: 17.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.561
IPC2_protein 5.614
IPC_protein 5.575
Toseland 5.83
ProMoST 5.868
Dawson 5.766
Bjellqvist 5.792
Wikipedia 5.741
Rodwell 5.741
Grimsley 5.906
Solomon 5.766
Lehninger 5.753
Nozaki 5.995
DTASelect 6.173
Thurlkill 6.148
EMBOSS 6.097
Sillero 6.097
Patrickios 4.113
IPC_peptide 5.779
IPC2_peptide 6.109
IPC2.peptide.svr19 6.131
Protein with the highest isoelectric point:
>tr|Q66009|Q66009_9VIRU ORF5 OS=Coconut foliar decay virus OX=12474 PE=4 SV=1
MM1 pKa = 6.87 RR2 pKa = 11.84 TRR4 pKa = 11.84 RR5 pKa = 11.84 RR6 pKa = 11.84 RR7 pKa = 11.84 RR8 pKa = 11.84 EE9 pKa = 3.58 VRR11 pKa = 11.84 VCQISRR17 pKa = 11.84 TQARR21 pKa = 11.84 LVLHH25 pKa = 6.37 MWWNQKK31 pKa = 8.92 GRR33 pKa = 11.84 IVPVHH38 pKa = 6.96 RR39 pKa = 11.84 GPKK42 pKa = 8.2 TKK44 pKa = 10.35 FNPRR48 pKa = 11.84 CTQVV52 pKa = 2.51
Molecular weight: 6.42 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.262
IPC2_protein 10.555
IPC_protein 12.135
Toseland 12.31
ProMoST 12.808
Dawson 12.31
Bjellqvist 12.31
Wikipedia 12.778
Rodwell 11.945
Grimsley 12.354
Solomon 12.808
Lehninger 12.705
Nozaki 12.31
DTASelect 12.31
Thurlkill 12.31
EMBOSS 12.808
Sillero 12.31
Patrickios 11.681
IPC_peptide 12.808
IPC2_peptide 11.798
IPC2.peptide.svr19 9.23
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
659
48
290
109.8
12.6
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.945 ± 0.683
2.883 ± 0.492
4.401 ± 0.936
6.07 ± 0.946
3.49 ± 0.836
6.677 ± 0.858
2.58 ± 0.326
5.918 ± 1.037
4.097 ± 0.506
9.256 ± 1.509
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.821 ± 0.836
4.097 ± 0.452
6.222 ± 0.621
4.097 ± 0.946
9.105 ± 1.593
6.525 ± 2.037
6.677 ± 2.065
6.829 ± 1.027
2.124 ± 0.393
3.187 ± 0.948
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here