Wilkie narna-like virus 1
Average proteome isoelectric point is 6.99
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1Z2RTD4|A0A1Z2RTD4_9VIRU RdRp OS=Wilkie narna-like virus 1 OX=2010280 PE=4 SV=1
MM1 pKa = 7.74 VSGLVDD7 pKa = 4.15 ALWCADD13 pKa = 3.24 EE14 pKa = 4.7 FVFVLGSIDD23 pKa = 4.92 LKK25 pKa = 9.35 TLKK28 pKa = 10.92 ALVRR32 pKa = 11.84 KK33 pKa = 8.93 IFCVGTFNLGLLVDD47 pKa = 5.45 DD48 pKa = 4.42 YY49 pKa = 11.47 KK50 pKa = 11.63 AWANRR55 pKa = 11.84 LFHH58 pKa = 6.89 KK59 pKa = 10.46 AASTQTIGQMEE70 pKa = 4.81 PLRR73 pKa = 11.84 CNNIFKK79 pKa = 9.87 MLDD82 pKa = 3.21 SIEE85 pKa = 4.22 YY86 pKa = 9.68 IKK88 pKa = 10.58 AIQDD92 pKa = 3.65 SGSDD96 pKa = 3.26 CSFINLQKK104 pKa = 10.62 LSHH107 pKa = 6.23 LVSSRR112 pKa = 11.84 QLPYY116 pKa = 9.94 MGLKK120 pKa = 9.47 TEE122 pKa = 4.13 IKK124 pKa = 9.82 SLKK127 pKa = 9.52 GFKK130 pKa = 10.41 DD131 pKa = 3.67 VVTSDD136 pKa = 3.42 YY137 pKa = 11.14 RR138 pKa = 11.84 PTLEE142 pKa = 4.66 TLDD145 pKa = 4.97 LLTKK149 pKa = 9.62 AARR152 pKa = 11.84 RR153 pKa = 11.84 IGGICRR159 pKa = 11.84 SIRR162 pKa = 11.84 KK163 pKa = 8.95 DD164 pKa = 3.38 RR165 pKa = 11.84 PIPDD169 pKa = 3.85 AEE171 pKa = 4.05 AHH173 pKa = 6.39 ISMTCSGEE181 pKa = 4.0 ATTSIIDD188 pKa = 3.71 GGQACAVRR196 pKa = 11.84 EE197 pKa = 4.1 GFEE200 pKa = 4.44 RR201 pKa = 11.84 IMGRR205 pKa = 11.84 IAEE208 pKa = 4.26 SDD210 pKa = 3.84 LLEE213 pKa = 4.45 DD214 pKa = 3.83 TPFGVASHH222 pKa = 6.89 RR223 pKa = 11.84 EE224 pKa = 4.63 GYY226 pKa = 10.48 ALWRR230 pKa = 11.84 TIFRR234 pKa = 11.84 EE235 pKa = 4.05 EE236 pKa = 4.01 PVEE239 pKa = 4.58 GGSLYY244 pKa = 10.9 DD245 pKa = 4.14 PLYY248 pKa = 10.35 QGYY251 pKa = 8.69 PKK253 pKa = 10.1 DD254 pKa = 3.47 QPGRR258 pKa = 11.84 YY259 pKa = 9.01 LGLDD263 pKa = 3.07 RR264 pKa = 11.84 VLGKK268 pKa = 10.15 QLLYY272 pKa = 10.7 VAWKK276 pKa = 10.3 EE277 pKa = 4.39 SILNPTIVLRR287 pKa = 11.84 ASIVPEE293 pKa = 3.98 MGNKK297 pKa = 9.42 ARR299 pKa = 11.84 VVTMSPYY306 pKa = 9.35 WVQLLQAPLAHH317 pKa = 6.05 LTIAGMRR324 pKa = 11.84 LHH326 pKa = 6.87 PSVFSSFARR335 pKa = 11.84 QDD337 pKa = 3.81 QAWEE341 pKa = 3.77 AAKK344 pKa = 10.71 GISRR348 pKa = 11.84 LNIDD352 pKa = 2.99 NWEE355 pKa = 4.28 DD356 pKa = 3.51 LHH358 pKa = 8.21 VLSSDD363 pKa = 4.66 LKK365 pKa = 11.03 DD366 pKa = 3.24 ATNAQQQLLTRR377 pKa = 11.84 TMLRR381 pKa = 11.84 AFMNGYY387 pKa = 10.71 GMIHH391 pKa = 7.02 RR392 pKa = 11.84 NTYY395 pKa = 10.47 SEE397 pKa = 4.65 LALSTIVEE405 pKa = 4.1 RR406 pKa = 11.84 LITFEE411 pKa = 4.62 DD412 pKa = 3.85 QTSVWATTGIMMGEE426 pKa = 4.86 PIAKK430 pKa = 9.76 PSLTLLNLAIEE441 pKa = 4.34 EE442 pKa = 4.44 LAFLTYY448 pKa = 9.69 TNRR451 pKa = 11.84 LDD453 pKa = 5.56 LLDD456 pKa = 4.72 SDD458 pKa = 5.23 LPSPYY463 pKa = 9.73 RR464 pKa = 11.84 AWRR467 pKa = 11.84 FIHH470 pKa = 7.04 IGGDD474 pKa = 3.2 DD475 pKa = 3.76 HH476 pKa = 7.89 LAIGPKK482 pKa = 9.86 KK483 pKa = 9.84 YY484 pKa = 10.79 LEE486 pKa = 5.43 LITNYY491 pKa = 10.18 HH492 pKa = 6.68 LLAGSHH498 pKa = 6.59 IDD500 pKa = 3.7 PGKK503 pKa = 10.1 HH504 pKa = 5.99 GYY506 pKa = 8.73 SQVAVKK512 pKa = 8.91 YY513 pKa = 7.0 TEE515 pKa = 5.27 RR516 pKa = 11.84 ILSVKK521 pKa = 9.77 NFRR524 pKa = 11.84 FKK526 pKa = 11.37 APFDD530 pKa = 3.77 YY531 pKa = 11.18 DD532 pKa = 3.78 NYY534 pKa = 7.81 PKK536 pKa = 11.15 SMIVDD541 pKa = 3.8 SVKK544 pKa = 10.22 VRR546 pKa = 11.84 LLEE549 pKa = 4.6 RR550 pKa = 11.84 GQSTMQKK557 pKa = 10.27 KK558 pKa = 10.14 DD559 pKa = 3.49 NKK561 pKa = 10.05 NVAIGKK567 pKa = 8.89 SRR569 pKa = 11.84 MMSGCLDD576 pKa = 3.86 WLPSDD581 pKa = 3.62 PAYY584 pKa = 9.81 WPSDD588 pKa = 3.0 KK589 pKa = 10.78 KK590 pKa = 10.86 ISIRR594 pKa = 11.84 NLFINRR600 pKa = 11.84 MGPLLPSKK608 pKa = 10.32 SLHH611 pKa = 5.66 PKK613 pKa = 9.86 CYY615 pKa = 10.0 HH616 pKa = 5.77 SVQLPSILGGFNLGLKK632 pKa = 9.99 SEE634 pKa = 4.73 IYY636 pKa = 9.49 QAYY639 pKa = 7.59 TLAPPPIRR647 pKa = 11.84 WVLNKK652 pKa = 9.92 AAAGVDD658 pKa = 3.54 VTAEE662 pKa = 4.06 LKK664 pKa = 10.46 ILSLLNRR671 pKa = 11.84 NISDD675 pKa = 3.6 RR676 pKa = 11.84 GTDD679 pKa = 3.36 PLQEE683 pKa = 3.9 YY684 pKa = 9.04 RR685 pKa = 11.84 ASIIDD690 pKa = 3.38 QLYY693 pKa = 10.45 SYY695 pKa = 8.79 PQLIGAITWKK705 pKa = 10.42 EE706 pKa = 3.9 ILSKK710 pKa = 10.66 FPPQNEE716 pKa = 3.87 NVRR719 pKa = 11.84 WSIYY723 pKa = 9.55 AARR726 pKa = 11.84 EE727 pKa = 3.69 AHH729 pKa = 6.07 YY730 pKa = 10.97 LSIEE734 pKa = 4.18 EE735 pKa = 3.92 FAEE738 pKa = 3.96 WSTRR742 pKa = 11.84 GNTFVQLIMGRR753 pKa = 11.84 GKK755 pKa = 10.45 NNVFNTRR762 pKa = 11.84 PYY764 pKa = 8.59 VRR766 pKa = 11.84 TFARR770 pKa = 11.84 VWTEE774 pKa = 3.89 LEE776 pKa = 3.85 ALQTDD781 pKa = 5.16 LYY783 pKa = 10.77 GDD785 pKa = 3.81 SPLGEE790 pKa = 4.25 GEE792 pKa = 4.09 FNKK795 pKa = 9.58 TLRR798 pKa = 11.84 GLSRR802 pKa = 11.84 CLYY805 pKa = 10.2 FDD807 pKa = 4.12 TSQSTTIDD815 pKa = 2.78 IGYY818 pKa = 8.9 YY819 pKa = 10.31 KK820 pKa = 10.24 PDD822 pKa = 3.89 DD823 pKa = 4.13 PDD825 pKa = 3.91 RR826 pKa = 11.84 EE827 pKa = 4.11 EE828 pKa = 4.89 FEE830 pKa = 5.3 FVDD833 pKa = 3.64 ATYY836 pKa = 11.26 LEE838 pKa = 4.68 AFDD841 pKa = 5.46 RR842 pKa = 11.84 MLPTLVVGKK851 pKa = 10.41 KK852 pKa = 10.34 FIGFRR857 pKa = 11.84 NN858 pKa = 3.55
Molecular weight: 97.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.99
IPC2_protein 7.424
IPC_protein 7.293
Toseland 7.102
ProMoST 7.834
Dawson 7.995
Bjellqvist 8.273
Wikipedia 7.936
Rodwell 8.024
Grimsley 7.19
Solomon 8.053
Lehninger 8.068
Nozaki 8.39
DTASelect 8.097
Thurlkill 8.141
EMBOSS 8.214
Sillero 8.419
Patrickios 4.317
IPC_peptide 8.053
IPC2_peptide 7.278
IPC2.peptide.svr19 7.457
Protein with the highest isoelectric point:
>tr|A0A1Z2RTD4|A0A1Z2RTD4_9VIRU RdRp OS=Wilkie narna-like virus 1 OX=2010280 PE=4 SV=1
MM1 pKa = 7.74 VSGLVDD7 pKa = 4.15 ALWCADD13 pKa = 3.24 EE14 pKa = 4.7 FVFVLGSIDD23 pKa = 4.92 LKK25 pKa = 9.35 TLKK28 pKa = 10.92 ALVRR32 pKa = 11.84 KK33 pKa = 8.93 IFCVGTFNLGLLVDD47 pKa = 5.45 DD48 pKa = 4.42 YY49 pKa = 11.47 KK50 pKa = 11.63 AWANRR55 pKa = 11.84 LFHH58 pKa = 6.89 KK59 pKa = 10.46 AASTQTIGQMEE70 pKa = 4.81 PLRR73 pKa = 11.84 CNNIFKK79 pKa = 9.87 MLDD82 pKa = 3.21 SIEE85 pKa = 4.22 YY86 pKa = 9.68 IKK88 pKa = 10.58 AIQDD92 pKa = 3.65 SGSDD96 pKa = 3.26 CSFINLQKK104 pKa = 10.62 LSHH107 pKa = 6.23 LVSSRR112 pKa = 11.84 QLPYY116 pKa = 9.94 MGLKK120 pKa = 9.47 TEE122 pKa = 4.13 IKK124 pKa = 9.82 SLKK127 pKa = 9.52 GFKK130 pKa = 10.41 DD131 pKa = 3.67 VVTSDD136 pKa = 3.42 YY137 pKa = 11.14 RR138 pKa = 11.84 PTLEE142 pKa = 4.66 TLDD145 pKa = 4.97 LLTKK149 pKa = 9.62 AARR152 pKa = 11.84 RR153 pKa = 11.84 IGGICRR159 pKa = 11.84 SIRR162 pKa = 11.84 KK163 pKa = 8.95 DD164 pKa = 3.38 RR165 pKa = 11.84 PIPDD169 pKa = 3.85 AEE171 pKa = 4.05 AHH173 pKa = 6.39 ISMTCSGEE181 pKa = 4.0 ATTSIIDD188 pKa = 3.71 GGQACAVRR196 pKa = 11.84 EE197 pKa = 4.1 GFEE200 pKa = 4.44 RR201 pKa = 11.84 IMGRR205 pKa = 11.84 IAEE208 pKa = 4.26 SDD210 pKa = 3.84 LLEE213 pKa = 4.45 DD214 pKa = 3.83 TPFGVASHH222 pKa = 6.89 RR223 pKa = 11.84 EE224 pKa = 4.63 GYY226 pKa = 10.48 ALWRR230 pKa = 11.84 TIFRR234 pKa = 11.84 EE235 pKa = 4.05 EE236 pKa = 4.01 PVEE239 pKa = 4.58 GGSLYY244 pKa = 10.9 DD245 pKa = 4.14 PLYY248 pKa = 10.35 QGYY251 pKa = 8.69 PKK253 pKa = 10.1 DD254 pKa = 3.47 QPGRR258 pKa = 11.84 YY259 pKa = 9.01 LGLDD263 pKa = 3.07 RR264 pKa = 11.84 VLGKK268 pKa = 10.15 QLLYY272 pKa = 10.7 VAWKK276 pKa = 10.3 EE277 pKa = 4.39 SILNPTIVLRR287 pKa = 11.84 ASIVPEE293 pKa = 3.98 MGNKK297 pKa = 9.42 ARR299 pKa = 11.84 VVTMSPYY306 pKa = 9.35 WVQLLQAPLAHH317 pKa = 6.05 LTIAGMRR324 pKa = 11.84 LHH326 pKa = 6.87 PSVFSSFARR335 pKa = 11.84 QDD337 pKa = 3.81 QAWEE341 pKa = 3.77 AAKK344 pKa = 10.71 GISRR348 pKa = 11.84 LNIDD352 pKa = 2.99 NWEE355 pKa = 4.28 DD356 pKa = 3.51 LHH358 pKa = 8.21 VLSSDD363 pKa = 4.66 LKK365 pKa = 11.03 DD366 pKa = 3.24 ATNAQQQLLTRR377 pKa = 11.84 TMLRR381 pKa = 11.84 AFMNGYY387 pKa = 10.71 GMIHH391 pKa = 7.02 RR392 pKa = 11.84 NTYY395 pKa = 10.47 SEE397 pKa = 4.65 LALSTIVEE405 pKa = 4.1 RR406 pKa = 11.84 LITFEE411 pKa = 4.62 DD412 pKa = 3.85 QTSVWATTGIMMGEE426 pKa = 4.86 PIAKK430 pKa = 9.76 PSLTLLNLAIEE441 pKa = 4.34 EE442 pKa = 4.44 LAFLTYY448 pKa = 9.69 TNRR451 pKa = 11.84 LDD453 pKa = 5.56 LLDD456 pKa = 4.72 SDD458 pKa = 5.23 LPSPYY463 pKa = 9.73 RR464 pKa = 11.84 AWRR467 pKa = 11.84 FIHH470 pKa = 7.04 IGGDD474 pKa = 3.2 DD475 pKa = 3.76 HH476 pKa = 7.89 LAIGPKK482 pKa = 9.86 KK483 pKa = 9.84 YY484 pKa = 10.79 LEE486 pKa = 5.43 LITNYY491 pKa = 10.18 HH492 pKa = 6.68 LLAGSHH498 pKa = 6.59 IDD500 pKa = 3.7 PGKK503 pKa = 10.1 HH504 pKa = 5.99 GYY506 pKa = 8.73 SQVAVKK512 pKa = 8.91 YY513 pKa = 7.0 TEE515 pKa = 5.27 RR516 pKa = 11.84 ILSVKK521 pKa = 9.77 NFRR524 pKa = 11.84 FKK526 pKa = 11.37 APFDD530 pKa = 3.77 YY531 pKa = 11.18 DD532 pKa = 3.78 NYY534 pKa = 7.81 PKK536 pKa = 11.15 SMIVDD541 pKa = 3.8 SVKK544 pKa = 10.22 VRR546 pKa = 11.84 LLEE549 pKa = 4.6 RR550 pKa = 11.84 GQSTMQKK557 pKa = 10.27 KK558 pKa = 10.14 DD559 pKa = 3.49 NKK561 pKa = 10.05 NVAIGKK567 pKa = 8.89 SRR569 pKa = 11.84 MMSGCLDD576 pKa = 3.86 WLPSDD581 pKa = 3.62 PAYY584 pKa = 9.81 WPSDD588 pKa = 3.0 KK589 pKa = 10.78 KK590 pKa = 10.86 ISIRR594 pKa = 11.84 NLFINRR600 pKa = 11.84 MGPLLPSKK608 pKa = 10.32 SLHH611 pKa = 5.66 PKK613 pKa = 9.86 CYY615 pKa = 10.0 HH616 pKa = 5.77 SVQLPSILGGFNLGLKK632 pKa = 9.99 SEE634 pKa = 4.73 IYY636 pKa = 9.49 QAYY639 pKa = 7.59 TLAPPPIRR647 pKa = 11.84 WVLNKK652 pKa = 9.92 AAAGVDD658 pKa = 3.54 VTAEE662 pKa = 4.06 LKK664 pKa = 10.46 ILSLLNRR671 pKa = 11.84 NISDD675 pKa = 3.6 RR676 pKa = 11.84 GTDD679 pKa = 3.36 PLQEE683 pKa = 3.9 YY684 pKa = 9.04 RR685 pKa = 11.84 ASIIDD690 pKa = 3.38 QLYY693 pKa = 10.45 SYY695 pKa = 8.79 PQLIGAITWKK705 pKa = 10.42 EE706 pKa = 3.9 ILSKK710 pKa = 10.66 FPPQNEE716 pKa = 3.87 NVRR719 pKa = 11.84 WSIYY723 pKa = 9.55 AARR726 pKa = 11.84 EE727 pKa = 3.69 AHH729 pKa = 6.07 YY730 pKa = 10.97 LSIEE734 pKa = 4.18 EE735 pKa = 3.92 FAEE738 pKa = 3.96 WSTRR742 pKa = 11.84 GNTFVQLIMGRR753 pKa = 11.84 GKK755 pKa = 10.45 NNVFNTRR762 pKa = 11.84 PYY764 pKa = 8.59 VRR766 pKa = 11.84 TFARR770 pKa = 11.84 VWTEE774 pKa = 3.89 LEE776 pKa = 3.85 ALQTDD781 pKa = 5.16 LYY783 pKa = 10.77 GDD785 pKa = 3.81 SPLGEE790 pKa = 4.25 GEE792 pKa = 4.09 FNKK795 pKa = 9.58 TLRR798 pKa = 11.84 GLSRR802 pKa = 11.84 CLYY805 pKa = 10.2 FDD807 pKa = 4.12 TSQSTTIDD815 pKa = 2.78 IGYY818 pKa = 8.9 YY819 pKa = 10.31 KK820 pKa = 10.24 PDD822 pKa = 3.89 DD823 pKa = 4.13 PDD825 pKa = 3.91 RR826 pKa = 11.84 EE827 pKa = 4.11 EE828 pKa = 4.89 FEE830 pKa = 5.3 FVDD833 pKa = 3.64 ATYY836 pKa = 11.26 LEE838 pKa = 4.68 AFDD841 pKa = 5.46 RR842 pKa = 11.84 MLPTLVVGKK851 pKa = 10.41 KK852 pKa = 10.34 FIGFRR857 pKa = 11.84 NN858 pKa = 3.55
Molecular weight: 97.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.99
IPC2_protein 7.424
IPC_protein 7.293
Toseland 7.102
ProMoST 7.834
Dawson 7.995
Bjellqvist 8.273
Wikipedia 7.936
Rodwell 8.024
Grimsley 7.19
Solomon 8.053
Lehninger 8.068
Nozaki 8.39
DTASelect 8.097
Thurlkill 8.141
EMBOSS 8.214
Sillero 8.419
Patrickios 4.317
IPC_peptide 8.053
IPC2_peptide 7.278
IPC2.peptide.svr19 7.457
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
858
858
858
858.0
97.07
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.876 ± 0.0
1.166 ± 0.0
5.944 ± 0.0
5.245 ± 0.0
3.963 ± 0.0
6.527 ± 0.0
1.865 ± 0.0
6.76 ± 0.0
5.478 ± 0.0
11.538 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.448 ± 0.0
3.846 ± 0.0
4.895 ± 0.0
3.38 ± 0.0
6.177 ± 0.0
7.226 ± 0.0
5.594 ± 0.0
5.012 ± 0.0
1.865 ± 0.0
4.196 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here