Fusobacterium naviforme
Average proteome isoelectric point is 6.21
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2187 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2P8EJE0|A0A2P8EJE0_9FUSO RNA polymerase primary sigma factor OS=Fusobacterium naviforme OX=77917 GN=EI53_01576 PE=4 SV=1
MM1 pKa = 7.86 EE2 pKa = 5.17 SRR4 pKa = 11.84 LDD6 pKa = 3.62 LSQASAEE13 pKa = 4.04 LAEE16 pKa = 4.45 EE17 pKa = 4.14 VRR19 pKa = 11.84 GEE21 pKa = 4.24 KK22 pKa = 10.62 SIDD25 pKa = 3.38 LDD27 pKa = 3.88 AMVSKK32 pKa = 10.23 LIPYY36 pKa = 9.37 GLYY39 pKa = 10.17 RR40 pKa = 11.84 KK41 pKa = 9.7 AQVDD45 pKa = 3.61 VANIFFEE52 pKa = 4.21 DD53 pKa = 3.27 ARR55 pKa = 11.84 RR56 pKa = 11.84 DD57 pKa = 3.8 FMIAEE62 pKa = 4.37 AEE64 pKa = 4.17 EE65 pKa = 4.4 GLGRR69 pKa = 11.84 ALADD73 pKa = 3.56 RR74 pKa = 11.84 EE75 pKa = 4.2 VRR77 pKa = 11.84 EE78 pKa = 4.75 LIIAAIGMSLGQEE91 pKa = 4.08 AEE93 pKa = 3.64 IDD95 pKa = 3.47 VCYY98 pKa = 10.67 ASEE101 pKa = 4.2 LSAAACVRR109 pKa = 11.84 LMSDD113 pKa = 3.31 DD114 pKa = 3.8 WEE116 pKa = 4.49 EE117 pKa = 4.14 SCEE120 pKa = 4.0 DD121 pKa = 4.14 LVSGACRR128 pKa = 11.84 DD129 pKa = 3.87 VEE131 pKa = 4.17 QLPLVDD137 pKa = 5.5 IYY139 pKa = 11.75 ACYY142 pKa = 10.34 EE143 pKa = 4.07 GDD145 pKa = 4.31 DD146 pKa = 3.66 GAFYY150 pKa = 11.14 DD151 pKa = 5.11 EE152 pKa = 4.84 IPEE155 pKa = 4.04 EE156 pKa = 4.35 AEE158 pKa = 3.9 PEE160 pKa = 4.08 DD161 pKa = 4.5 FRR163 pKa = 11.84 EE164 pKa = 4.26 LTAADD169 pKa = 3.34 IFFCEE174 pKa = 4.53 GAEE177 pKa = 3.74 PDD179 pKa = 4.1 YY180 pKa = 11.08 IEE182 pKa = 5.46 EE183 pKa = 4.12 ILEE186 pKa = 4.13 VMSVPEE192 pKa = 3.97 HH193 pKa = 6.39 HH194 pKa = 7.3 SYY196 pKa = 11.28 DD197 pKa = 3.81 YY198 pKa = 11.21 LVPEE202 pKa = 5.07 LMLSDD207 pKa = 5.26 DD208 pKa = 4.37 EE209 pKa = 5.59 DD210 pKa = 6.35 GEE212 pKa = 4.32 MEE214 pKa = 3.99 MAEE217 pKa = 4.2 EE218 pKa = 4.31 EE219 pKa = 4.52 EE220 pKa = 4.33 ILL222 pKa = 4.49
Molecular weight: 24.91 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.764
IPC2_protein 3.745
IPC_protein 3.719
Toseland 3.528
ProMoST 3.846
Dawson 3.681
Bjellqvist 3.834
Wikipedia 3.567
Rodwell 3.554
Grimsley 3.439
Solomon 3.668
Lehninger 3.63
Nozaki 3.795
DTASelect 3.948
Thurlkill 3.554
EMBOSS 3.579
Sillero 3.834
Patrickios 1.812
IPC_peptide 3.681
IPC2_peptide 3.821
IPC2.peptide.svr19 3.761
Protein with the highest isoelectric point:
>tr|A0A2P8ENG1|A0A2P8ENG1_9FUSO Ribosomal RNA small subunit methyltransferase G OS=Fusobacterium naviforme OX=77917 GN=rsmG PE=3 SV=1
MM1 pKa = 7.92 RR2 pKa = 11.84 KK3 pKa = 8.48 GTMTFQPHH11 pKa = 5.66 NAQKK15 pKa = 10.45 FRR17 pKa = 11.84 VHH19 pKa = 6.5 GFRR22 pKa = 11.84 ARR24 pKa = 11.84 MATKK28 pKa = 10.36 GGRR31 pKa = 11.84 KK32 pKa = 8.97 VLAARR37 pKa = 11.84 RR38 pKa = 11.84 AKK40 pKa = 10.26 GRR42 pKa = 11.84 AKK44 pKa = 10.1 LTVV47 pKa = 3.04
Molecular weight: 5.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.494
IPC2_protein 11.111
IPC_protein 12.691
Toseland 12.852
ProMoST 13.349
Dawson 12.852
Bjellqvist 12.852
Wikipedia 13.334
Rodwell 12.618
Grimsley 12.896
Solomon 13.349
Lehninger 13.247
Nozaki 12.852
DTASelect 12.852
Thurlkill 12.852
EMBOSS 13.349
Sillero 12.852
Patrickios 12.34
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.103
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2187
0
2187
714170
39
3134
326.6
36.35
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.069 ± 0.065
1.462 ± 0.02
5.173 ± 0.042
7.964 ± 0.058
3.862 ± 0.034
7.596 ± 0.043
1.808 ± 0.024
6.348 ± 0.048
5.343 ± 0.052
9.824 ± 0.063
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.901 ± 0.025
3.563 ± 0.034
3.634 ± 0.033
3.218 ± 0.027
6.041 ± 0.053
6.106 ± 0.043
5.033 ± 0.033
6.522 ± 0.041
0.905 ± 0.018
3.626 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here