Mycobacterium phage Bones

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fromanvirus; unclassified Fromanvirus

Average proteome isoelectric point is 6.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 89 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3G8FVT3|A0A3G8FVT3_9CAUD Uncharacterized protein OS=Mycobacterium phage Bones OX=2488963 GN=83 PE=4 SV=1
MM1 pKa = 7.06YY2 pKa = 10.59VVTDD6 pKa = 4.24PDD8 pKa = 3.65GDD10 pKa = 4.04EE11 pKa = 4.22YY12 pKa = 11.25TVTDD16 pKa = 3.96TEE18 pKa = 5.99FEE20 pKa = 4.65DD21 pKa = 3.12GWYY24 pKa = 9.34WVVSPLSEE32 pKa = 4.67FAPCHH37 pKa = 6.69LDD39 pKa = 3.74LGLHH43 pKa = 4.69RR44 pKa = 11.84TKK46 pKa = 10.64RR47 pKa = 11.84DD48 pKa = 3.07AVAALAEE55 pKa = 4.56FVSS58 pKa = 4.03

Molecular weight: 6.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3G8FVP3|A0A3G8FVP3_9CAUD Portal protein OS=Mycobacterium phage Bones OX=2488963 GN=12 PE=4 SV=1
MM1 pKa = 7.2SRR3 pKa = 11.84IVLRR7 pKa = 11.84SYY9 pKa = 11.36VKK11 pKa = 9.07ITEE14 pKa = 4.03VSTGCKK20 pKa = 9.8FNDD23 pKa = 3.73WEE25 pKa = 4.25WVYY28 pKa = 10.83RR29 pKa = 11.84PWMPLWFQKK38 pKa = 10.4QLIVRR43 pKa = 11.84PLWKK47 pKa = 9.5RR48 pKa = 11.84HH49 pKa = 4.42KK50 pKa = 9.68RR51 pKa = 11.84NCIRR55 pKa = 11.84AEE57 pKa = 3.64HH58 pKa = 5.88WRR60 pKa = 11.84AMDD63 pKa = 3.45

Molecular weight: 7.98 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

89

0

89

16379

31

886

184.0

20.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.995 ± 0.395

0.672 ± 0.123

6.698 ± 0.197

6.453 ± 0.299

3.236 ± 0.147

8.664 ± 0.524

1.899 ± 0.166

4.811 ± 0.206

4.304 ± 0.245

8.486 ± 0.243

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.996 ± 0.11

3.279 ± 0.156

5.617 ± 0.218

3.291 ± 0.193

6.27 ± 0.432

5.879 ± 0.239

6.24 ± 0.294

7.388 ± 0.255

1.942 ± 0.126

2.882 ± 0.169
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski