Streptococcus satellite phage Javan120

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 6.92

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 25 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZF12|A0A4D5ZF12_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan120 OX=2558530 GN=JavanS120_0016 PE=4 SV=1
MM1 pKa = 7.55RR2 pKa = 11.84TFSDD6 pKa = 3.67TPKK9 pKa = 10.21TFTFHH14 pKa = 5.7YY15 pKa = 8.55TFKK18 pKa = 11.1DD19 pKa = 3.09FDD21 pKa = 4.09TAQVACHH28 pKa = 6.88AILGYY33 pKa = 7.77MTGTYY38 pKa = 7.11EE39 pKa = 4.76QPVIDD44 pKa = 3.97ATYY47 pKa = 10.82HH48 pKa = 6.24NDD50 pKa = 3.59DD51 pKa = 3.72QGGHH55 pKa = 6.59ANQLVLEE62 pKa = 4.22YY63 pKa = 11.31AEE65 pKa = 3.86NRR67 pKa = 11.84KK68 pKa = 9.61LNKK71 pKa = 9.07VFKK74 pKa = 10.18RR75 pKa = 11.84ICDD78 pKa = 3.69SFKK81 pKa = 10.93DD82 pKa = 4.21YY83 pKa = 11.52YY84 pKa = 10.51NQPEE88 pKa = 4.58DD89 pKa = 3.61MTDD92 pKa = 4.14DD93 pKa = 4.37EE94 pKa = 6.25LDD96 pKa = 4.42DD97 pKa = 3.69MAQEE101 pKa = 4.07NEE103 pKa = 4.3LIKK106 pKa = 10.59EE107 pKa = 4.24VEE109 pKa = 4.36EE110 pKa = 4.59PDD112 pKa = 3.37HH113 pKa = 6.22QRR115 pKa = 11.84VVSLSKK121 pKa = 9.84STQEE125 pKa = 4.12KK126 pKa = 10.62VNDD129 pKa = 4.22RR130 pKa = 11.84DD131 pKa = 3.35TFMAFISNHH140 pKa = 4.84NQLAEE145 pKa = 4.24HH146 pKa = 6.92LSMNYY151 pKa = 10.19KK152 pKa = 10.52EE153 pKa = 4.5MTPEE157 pKa = 3.92DD158 pKa = 3.6LGAILEE164 pKa = 4.71SISQAFNHH172 pKa = 6.8LYY174 pKa = 10.59DD175 pKa = 3.68IVVEE179 pKa = 4.3GQLVVKK185 pKa = 10.63

Molecular weight: 21.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZE73|A0A4D5ZE73_9VIRU Putative transcriptional regulator OS=Streptococcus satellite phage Javan120 OX=2558530 GN=JavanS120_0021 PE=4 SV=1
MM1 pKa = 7.49AGSFRR6 pKa = 11.84QHH8 pKa = 6.21HH9 pKa = 6.18PAFDD13 pKa = 3.68NMLLADD19 pKa = 3.8DD20 pKa = 5.86RR21 pKa = 11.84IYY23 pKa = 11.27KK24 pKa = 9.51NRR26 pKa = 11.84RR27 pKa = 11.84PTIEE31 pKa = 3.9QNKK34 pKa = 7.86EE35 pKa = 3.61QQHH38 pKa = 5.43LKK40 pKa = 10.3KK41 pKa = 10.5LKK43 pKa = 8.78KK44 pKa = 9.76KK45 pKa = 9.78RR46 pKa = 11.84RR47 pKa = 11.84KK48 pKa = 9.22GQQ50 pKa = 3.06

Molecular weight: 6.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

25

0

25

3599

40

562

144.0

16.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.974 ± 0.434

0.695 ± 0.228

5.39 ± 0.496

7.947 ± 0.584

4.251 ± 0.289

4.779 ± 0.353

2.112 ± 0.331

7.113 ± 0.345

8.586 ± 0.484

10.142 ± 0.56

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.584 ± 0.297

5.39 ± 0.264

3.362 ± 0.489

4.668 ± 0.29

5.529 ± 0.331

5.057 ± 0.339

6.085 ± 0.429

4.946 ± 0.351

0.861 ± 0.12

4.529 ± 0.386
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski