Melghirimyces algeriensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Thermoactinomycetaceae; Melghirimyces

Average proteome isoelectric point is 6.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3293 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A521FA23|A0A521FA23_9BACL Uncharacterized protein OS=Melghirimyces algeriensis OX=910412 GN=SAMN06264849_11522 PE=4 SV=1
MM1 pKa = 7.46HH2 pKa = 6.83VRR4 pKa = 11.84FVDD7 pKa = 3.67IVYY10 pKa = 10.61SGGFEE15 pKa = 4.19EE16 pKa = 5.61YY17 pKa = 10.26EE18 pKa = 4.3DD19 pKa = 3.78PADD22 pKa = 3.61YY23 pKa = 10.85KK24 pKa = 11.08RR25 pKa = 11.84IVSTLAYY32 pKa = 10.17SDD34 pKa = 4.12TYY36 pKa = 11.5DD37 pKa = 3.57EE38 pKa = 4.4EE39 pKa = 4.43

Molecular weight: 4.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A521D4K3|A0A521D4K3_9BACL Membrane protein involved in the export of O-antigen and teichoic acid OS=Melghirimyces algeriensis OX=910412 GN=SAMN06264849_105115 PE=4 SV=1
MM1 pKa = 7.98WIPSFLRR8 pKa = 11.84QRR10 pKa = 11.84FSGFISGAGRR20 pKa = 11.84WIKK23 pKa = 10.41LFQRR27 pKa = 11.84GRR29 pKa = 11.84RR30 pKa = 11.84YY31 pKa = 7.33MQRR34 pKa = 11.84LMRR37 pKa = 11.84FFGKK41 pKa = 10.04LRR43 pKa = 11.84SLQSKK48 pKa = 8.99LKK50 pKa = 9.48PLHH53 pKa = 7.62SMMKK57 pKa = 10.58AFII60 pKa = 4.29

Molecular weight: 7.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3293

0

3293

984518

29

6202

299.0

33.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.111 ± 0.044

0.86 ± 0.012

5.104 ± 0.037

7.35 ± 0.045

3.993 ± 0.035

7.468 ± 0.039

2.4 ± 0.024

6.32 ± 0.04

5.919 ± 0.054

10.034 ± 0.059

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.857 ± 0.02

3.364 ± 0.027

4.312 ± 0.029

4.275 ± 0.036

5.746 ± 0.037

5.817 ± 0.033

5.16 ± 0.031

7.445 ± 0.034

1.363 ± 0.019

3.102 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski