Mytilinidion resinicola
Average proteome isoelectric point is 6.75
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 16739 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6A6YVN4|A0A6A6YVN4_9PEZI WD repeat-containing protein 57 OS=Mytilinidion resinicola OX=574789 GN=BDZ99DRAFT_460330 PE=4 SV=1
MM1 pKa = 7.53 HH2 pKa = 7.19 SLSTFACRR10 pKa = 11.84 IGLLALTARR19 pKa = 11.84 SALADD24 pKa = 3.63 CVSFGMDD31 pKa = 3.43 FQDD34 pKa = 4.05 GGSYY38 pKa = 9.79 FQNSLSSDD46 pKa = 3.21 PFTFVSQFEE55 pKa = 4.48 GCDD58 pKa = 3.01 NDD60 pKa = 3.6 TATNVFVDD68 pKa = 4.08 PNGDD72 pKa = 3.22 QYY74 pKa = 12.04 LCTDD78 pKa = 3.99 TNLQPDD84 pKa = 4.14 DD85 pKa = 3.8 TDD87 pKa = 3.9 EE88 pKa = 6.09 LSTCPLDD95 pKa = 5.78 KK96 pKa = 10.95 DD97 pKa = 4.24 QLWSGPWSILILSNNGNDD115 pKa = 3.45 APIAYY120 pKa = 9.29 EE121 pKa = 4.0 RR122 pKa = 11.84 DD123 pKa = 3.55 FEE125 pKa = 5.43 LSVGPQNTSTVCFPSLLSTQVVTNEE150 pKa = 3.99 RR151 pKa = 11.84 SSTHH155 pKa = 5.45 PQVFQKK161 pKa = 9.95 TSS163 pKa = 2.7
Molecular weight: 17.81 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.733
IPC2_protein 3.783
IPC_protein 3.783
Toseland 3.554
ProMoST 3.973
Dawson 3.795
Bjellqvist 3.948
Wikipedia 3.77
Rodwell 3.605
Grimsley 3.465
Solomon 3.783
Lehninger 3.732
Nozaki 3.91
DTASelect 4.19
Thurlkill 3.617
EMBOSS 3.77
Sillero 3.91
Patrickios 0.655
IPC_peptide 3.77
IPC2_peptide 3.872
IPC2.peptide.svr19 3.806
Protein with the highest isoelectric point:
>tr|A0A6A6Z455|A0A6A6Z455_9PEZI Tyrosinase OS=Mytilinidion resinicola OX=574789 GN=BDZ99DRAFT_379186 PE=3 SV=1
MM1 pKa = 7.14 QLISPMTISMPTAGSLSTALSPLSAPRR28 pKa = 11.84 MLPTMTPKK36 pKa = 9.73 TRR38 pKa = 11.84 SLRR41 pKa = 11.84 AVFLLKK47 pKa = 10.28 KK48 pKa = 9.92 HH49 pKa = 4.73 QTLPPRR55 pKa = 11.84 RR56 pKa = 11.84 LHH58 pKa = 6.73 RR59 pKa = 11.84 PPPQPLHH66 pKa = 6.09 HH67 pKa = 6.64 QPLSQPLPQHH77 pKa = 6.7 LARR80 pKa = 11.84 PLQPLRR86 pKa = 11.84 SLKK89 pKa = 9.6 QQQLHH94 pKa = 5.98 SPKK97 pKa = 9.72 QQPSRR102 pKa = 11.84 LLKK105 pKa = 9.86 RR106 pKa = 11.84 QRR108 pKa = 11.84 IQLPRR113 pKa = 11.84 KK114 pKa = 9.09 HH115 pKa = 6.53 RR116 pKa = 11.84 LHH118 pKa = 7.1 PSRR121 pKa = 11.84 LLSPPQHH128 pKa = 5.9 RR129 pKa = 11.84 RR130 pKa = 11.84 PRR132 pKa = 11.84 RR133 pKa = 11.84 HH134 pKa = 4.71 QNQRR138 pKa = 11.84 QRR140 pKa = 11.84 PRR142 pKa = 11.84 QPHH145 pKa = 5.01 WQHH148 pKa = 5.93 KK149 pKa = 9.37 QPLHH153 pKa = 5.11 PTRR156 pKa = 11.84 VALCPHH162 pKa = 7.19 RR163 pKa = 11.84 LTRR166 pKa = 11.84 QTRR169 pKa = 11.84 SASHH173 pKa = 4.61 QQGLQKK179 pKa = 10.29 LRR181 pKa = 11.84 KK182 pKa = 8.47 PRR184 pKa = 11.84 QEE186 pKa = 4.26 PPPKK190 pKa = 9.7 PRR192 pKa = 11.84 QQAPGPRR199 pKa = 11.84 RR200 pKa = 3.32
Molecular weight: 23.47 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.199
IPC_protein 12.793
Toseland 12.954
ProMoST 13.451
Dawson 12.954
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.603
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.954
DTASelect 12.954
Thurlkill 12.954
EMBOSS 13.451
Sillero 12.954
Patrickios 12.31
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.169
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
16739
0
16739
6754280
49
7174
403.5
44.7
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.749 ± 0.017
1.334 ± 0.008
5.497 ± 0.014
6.214 ± 0.021
3.735 ± 0.011
6.803 ± 0.018
2.467 ± 0.009
4.896 ± 0.013
4.949 ± 0.018
9.006 ± 0.02
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.121 ± 0.007
3.558 ± 0.011
6.146 ± 0.022
3.813 ± 0.015
6.235 ± 0.017
8.132 ± 0.023
5.999 ± 0.012
6.049 ± 0.014
1.536 ± 0.007
2.761 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here