Lactobacillus rhamnosus MTCC 5462

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae; Lacticaseibacillus; Lacticaseibacillus rhamnosus

Average proteome isoelectric point is 6.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3249 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F3MZ03|F3MZ03_LACRH Chloride channel protein (Fragment) OS=Lactobacillus rhamnosus MTCC 5462 OX=947828 GN=AAULR_07136 PE=4 SV=1
MM1 pKa = 7.82DD2 pKa = 6.62AILMSLMTALNEE14 pKa = 3.88HH15 pKa = 6.72LLINVYY21 pKa = 10.28QRR23 pKa = 11.84DD24 pKa = 3.45TDD26 pKa = 3.79DD27 pKa = 4.99FYY29 pKa = 11.19TGYY32 pKa = 10.53VQVLGHH38 pKa = 6.81DD39 pKa = 3.73AVVLGTYY46 pKa = 9.83NDD48 pKa = 3.4SGIADD53 pKa = 4.46GCALIAFSAIDD64 pKa = 3.48QVEE67 pKa = 4.05FAGDD71 pKa = 3.89DD72 pKa = 3.9LDD74 pKa = 4.35NMNFRR79 pKa = 11.84IDD81 pKa = 3.72LAQSEE86 pKa = 4.93HH87 pKa = 6.69FLTLQGKK94 pKa = 5.4EE95 pKa = 4.01TPYY98 pKa = 11.17KK99 pKa = 10.46FDD101 pKa = 3.32ATKK104 pKa = 10.84SS105 pKa = 3.51

Molecular weight: 11.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F3MW76|F3MW76_LACRH Uncharacterized protein OS=Lactobacillus rhamnosus MTCC 5462 OX=947828 GN=AAULR_24181 PE=4 SV=1
MM1 pKa = 5.72TTKK4 pKa = 9.85RR5 pKa = 11.84TFQPKK10 pKa = 8.33KK11 pKa = 7.5RR12 pKa = 11.84HH13 pKa = 5.56RR14 pKa = 11.84EE15 pKa = 3.79RR16 pKa = 11.84VHH18 pKa = 7.7GFMKK22 pKa = 10.54RR23 pKa = 11.84MSKK26 pKa = 10.63KK27 pKa = 10.02NGRR30 pKa = 11.84KK31 pKa = 8.68VLARR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84AKK39 pKa = 9.94GRR41 pKa = 11.84KK42 pKa = 8.67VLSAA46 pKa = 4.05

Molecular weight: 5.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3249

0

3249

409736

19

857

126.1

13.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.679 ± 0.076

0.57 ± 0.014

5.495 ± 0.056

4.54 ± 0.051

4.226 ± 0.043

6.878 ± 0.052

2.358 ± 0.028

6.573 ± 0.05

5.445 ± 0.051

10.292 ± 0.066

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.884 ± 0.03

4.048 ± 0.032

4.102 ± 0.037

4.706 ± 0.046

4.665 ± 0.044

5.696 ± 0.06

6.271 ± 0.049

7.198 ± 0.052

1.216 ± 0.025

3.149 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski