Rubrobacter xylanophilus (strain DSM 9941 / NBRC 16129 / PRD-1)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Rubrobacteria; Rubrobacterales; Rubrobacteraceae; Rubrobacter; Rubrobacter xylanophilus

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3127 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q1ASL9|Q1ASL9_RUBXD Uncharacterized protein OS=Rubrobacter xylanophilus (strain DSM 9941 / NBRC 16129 / PRD-1) OX=266117 GN=Rxyl_2692 PE=4 SV=1
MM1 pKa = 7.27LVAAMLAMVLVAAAPALAQANAVAGDD27 pKa = 3.64VQVQYY32 pKa = 9.47QEE34 pKa = 4.33CTQLIQALGEE44 pKa = 3.78QGQYY48 pKa = 11.01GDD50 pKa = 4.38AGAAAGDD57 pKa = 3.7IGSASAAEE65 pKa = 4.22IAQEE69 pKa = 4.13LGISVDD75 pKa = 3.44AVQNCLQAGGDD86 pKa = 3.64INVTEE91 pKa = 4.85GGVQVVLPSGEE102 pKa = 4.35VVTVPSGGAAAGGAAAGAAAGAAGAAAGGVLPEE135 pKa = 4.36TGGASLIALGAGALLVAGGLLARR158 pKa = 11.84RR159 pKa = 11.84IVRR162 pKa = 3.63

Molecular weight: 15.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q1AR66|Q1AR66_RUBXD Lytic transglycosylase catalytic OS=Rubrobacter xylanophilus (strain DSM 9941 / NBRC 16129 / PRD-1) OX=266117 GN=Rxyl_3208 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.4RR3 pKa = 11.84TYY5 pKa = 10.21QPNRR9 pKa = 11.84RR10 pKa = 11.84KK11 pKa = 9.78RR12 pKa = 11.84AKK14 pKa = 8.45THH16 pKa = 5.35GFRR19 pKa = 11.84KK20 pKa = 9.92RR21 pKa = 11.84MSSVGGRR28 pKa = 11.84RR29 pKa = 11.84IIAARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 8.91RR41 pKa = 11.84LAVV44 pKa = 3.5

Molecular weight: 5.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3127

0

3127

982021

44

1618

314.0

34.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.945 ± 0.067

0.808 ± 0.014

4.358 ± 0.033

8.351 ± 0.054

3.329 ± 0.029

10.301 ± 0.037

1.772 ± 0.021

3.703 ± 0.034

2.029 ± 0.025

11.002 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.945 ± 0.018

1.858 ± 0.019

5.602 ± 0.03

2.094 ± 0.022

9.674 ± 0.056

4.963 ± 0.023

4.052 ± 0.025

8.426 ± 0.039

1.24 ± 0.018

2.548 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski