Rat cytomegalovirus (isolate England) (RCMV-E) (Murid herpesvirus 8)

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Peploviricota; Herviviricetes; Herpesvirales; Herpesviridae; Betaherpesvirinae; Muromegalovirus

Average proteome isoelectric point is 6.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 137 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K7YA71|K7YA71_RCMVE EORF6 OS=Rat cytomegalovirus (isolate England) OX=1261657 GN=eORF6 PE=4 SV=1
MM1 pKa = 7.16TRR3 pKa = 11.84FIILSCVLCIAISSVATTMAPNVSGNDD30 pKa = 3.3MYY32 pKa = 11.29FEE34 pKa = 4.5NSGSGSSEE42 pKa = 3.74YY43 pKa = 10.74DD44 pKa = 4.04DD45 pKa = 5.13EE46 pKa = 5.39DD47 pKa = 4.35LSSGEE52 pKa = 4.14SDD54 pKa = 3.4MNSTILTEE62 pKa = 4.18NMEE65 pKa = 4.5STSNASDD72 pKa = 3.34GTDD75 pKa = 3.01EE76 pKa = 5.08YY77 pKa = 10.09NTQVTTFPNDD87 pKa = 2.76VTTDD91 pKa = 3.95FTLTPNNNYY100 pKa = 8.14STLSDD105 pKa = 3.34NRR107 pKa = 11.84SPEE110 pKa = 4.28TKK112 pKa = 10.58NPTDD116 pKa = 3.68TQTSTTPTEE125 pKa = 3.84TSISIKK131 pKa = 10.14TPTQTSAQMIQLTNTPTPTEE151 pKa = 4.1LPNTEE156 pKa = 4.38SPSLTTEE163 pKa = 3.97AVTSMKK169 pKa = 10.27TYY171 pKa = 10.29ILTSRR176 pKa = 11.84EE177 pKa = 4.09SSSKK181 pKa = 10.17SSEE184 pKa = 4.23DD185 pKa = 3.06IKK187 pKa = 11.3SEE189 pKa = 4.28NITSTSNTVYY199 pKa = 9.21KK200 pKa = 10.71TSDD203 pKa = 3.17TDD205 pKa = 3.43VFTYY209 pKa = 8.76QTTNASPTTISTEE222 pKa = 4.04TSVPTTPDD230 pKa = 3.16TKK232 pKa = 11.14SLDD235 pKa = 3.9SNKK238 pKa = 8.56VTDD241 pKa = 4.39SVTGTNSTMVTNNSSATIHH260 pKa = 6.53ASTPISHH267 pKa = 7.33TSTVVYY273 pKa = 8.7ITPHH277 pKa = 6.73NITLPDD283 pKa = 3.5TSTVSIAPHH292 pKa = 6.78NITSFDD298 pKa = 3.46TSTVSTIPHH307 pKa = 5.49VTPSDD312 pKa = 3.73YY313 pKa = 11.51NHH315 pKa = 7.12NITNHH320 pKa = 5.4NVTLYY325 pKa = 10.82DD326 pKa = 3.61YY327 pKa = 10.49DD328 pKa = 4.07YY329 pKa = 11.68KK330 pKa = 11.29NLQTTSSVSVSDD342 pKa = 4.86LYY344 pKa = 10.84ISTTIQTTPLFTPVPCISNYY364 pKa = 8.24TQDD367 pKa = 3.49INTSLALSSFDD378 pKa = 3.34HH379 pKa = 5.54VTFTRR384 pKa = 11.84VDD386 pKa = 3.82CPTDD390 pKa = 2.89IAAFDD395 pKa = 3.68KK396 pKa = 11.34GLVIDD401 pKa = 4.47SGLGASLFITIYY413 pKa = 10.57KK414 pKa = 10.14RR415 pKa = 11.84LNLVTKK421 pKa = 7.94MLRR424 pKa = 11.84LVEE427 pKa = 4.12EE428 pKa = 4.46LNEE431 pKa = 4.11YY432 pKa = 10.02TYY434 pKa = 11.01TPADD438 pKa = 3.71FVQQ441 pKa = 3.78

Molecular weight: 47.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K7YNI0|K7YNI0_RCMVE E31 OS=Rat cytomegalovirus (isolate England) OX=1261657 GN=E31 PE=3 SV=1
MM1 pKa = 7.54TSMCVRR7 pKa = 11.84RR8 pKa = 11.84EE9 pKa = 3.55LFAPHH14 pKa = 6.9RR15 pKa = 11.84SGVEE19 pKa = 3.56NHH21 pKa = 5.91PRR23 pKa = 11.84AMNGKK28 pKa = 9.58GVDD31 pKa = 3.52NPNRR35 pKa = 11.84SLPVSRR41 pKa = 11.84RR42 pKa = 11.84SRR44 pKa = 11.84AMGKK48 pKa = 8.66MGEE51 pKa = 4.5TTGSRR56 pKa = 11.84MRR58 pKa = 11.84VSTVFALACVVVIAVTALPGADD80 pKa = 2.79AVVDD84 pKa = 3.92AWSTGCEE91 pKa = 3.97PARR94 pKa = 11.84IGQMCKK100 pKa = 8.82MYY102 pKa = 11.02ARR104 pKa = 11.84ASRR107 pKa = 11.84GNIPSPPPQYY117 pKa = 10.81VSWSRR122 pKa = 11.84IGASSRR128 pKa = 11.84NEE130 pKa = 3.63VLLLSRR136 pKa = 11.84TEE138 pKa = 4.23VPWLGPPIKK147 pKa = 9.69TRR149 pKa = 11.84LPRR152 pKa = 11.84KK153 pKa = 9.37SGMRR157 pKa = 11.84GITGTAVSSKK167 pKa = 10.84GVVTSALIFNVTKK180 pKa = 10.04NTDD183 pKa = 1.92GWYY186 pKa = 9.63RR187 pKa = 11.84CNYY190 pKa = 9.2YY191 pKa = 10.72LDD193 pKa = 4.03GSVAEE198 pKa = 3.92FHH200 pKa = 7.38VNITAAATEE209 pKa = 4.32GGKK212 pKa = 9.95

Molecular weight: 22.84 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

136

1

137

55952

55

2029

408.4

45.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.962 ± 0.202

2.418 ± 0.107

6.075 ± 0.141

5.392 ± 0.135

4.339 ± 0.138

5.601 ± 0.268

2.407 ± 0.089

6.061 ± 0.194

4.624 ± 0.14

8.632 ± 0.201

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.42 ± 0.08

4.438 ± 0.124

4.86 ± 0.157

2.731 ± 0.121

6.908 ± 0.185

8.377 ± 0.212

6.836 ± 0.256

7.326 ± 0.181

1.013 ± 0.07

3.58 ± 0.111
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski