Marinactinospora thermotolerans DSM 45154

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptosporangiales; Nocardiopsaceae; Marinactinospora; Marinactinospora thermotolerans

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4956 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1T4RVP6|A0A1T4RVP6_9ACTN Uncharacterized protein OS=Marinactinospora thermotolerans DSM 45154 OX=1122192 GN=SAMN02745673_03036 PE=4 SV=1
MM1 pKa = 6.54TTPARR6 pKa = 11.84RR7 pKa = 11.84RR8 pKa = 11.84ATIAASLPLLLLAGGCSYY26 pKa = 11.38LSGEE30 pKa = 4.43SQGADD35 pKa = 3.61PQSADD40 pKa = 3.48CDD42 pKa = 4.84AYY44 pKa = 10.31EE45 pKa = 3.79QWQGHH50 pKa = 7.05DD51 pKa = 3.46GTTVSIYY58 pKa = 11.11ASIRR62 pKa = 11.84DD63 pKa = 3.67QEE65 pKa = 4.58AEE67 pKa = 4.05LLEE70 pKa = 4.42EE71 pKa = 4.25SWSEE75 pKa = 3.82FSSCTGIDD83 pKa = 2.89IAYY86 pKa = 8.16EE87 pKa = 3.97GSGEE91 pKa = 4.14FEE93 pKa = 3.86AQIQVKK99 pKa = 9.99VDD101 pKa = 3.32GGNAPDD107 pKa = 4.45LAFFPQPGLLARR119 pKa = 11.84FAQSGDD125 pKa = 3.6AIALPEE131 pKa = 4.21SVRR134 pKa = 11.84ANAEE138 pKa = 4.4SGWSEE143 pKa = 3.81DD144 pKa = 2.95WLNYY148 pKa = 8.62ATVDD152 pKa = 3.45GEE154 pKa = 5.15LYY156 pKa = 8.28GTPLGANVKK165 pKa = 10.15SFVWYY170 pKa = 10.57SPGLFSDD177 pKa = 3.81NGYY180 pKa = 8.19ATPTTWDD187 pKa = 3.54EE188 pKa = 4.48LIEE191 pKa = 4.78ISDD194 pKa = 3.67AMVEE198 pKa = 4.25DD199 pKa = 6.26GIKK202 pKa = 9.4PWCAGIEE209 pKa = 4.21SGDD212 pKa = 3.58ATGWPATDD220 pKa = 2.91WVEE223 pKa = 4.35NIMLRR228 pKa = 11.84EE229 pKa = 4.37HH230 pKa = 6.51GPEE233 pKa = 5.41VYY235 pKa = 10.23DD236 pKa = 2.98QWVDD240 pKa = 3.28HH241 pKa = 6.96EE242 pKa = 5.2IPFDD246 pKa = 4.21DD247 pKa = 4.45PAVAEE252 pKa = 4.18ALDD255 pKa = 3.96RR256 pKa = 11.84VDD258 pKa = 5.22SILRR262 pKa = 11.84NPDD265 pKa = 3.36YY266 pKa = 11.7VNGGHH271 pKa = 7.0GEE273 pKa = 4.19VQSIATTSFQDD284 pKa = 3.0AGTPILDD291 pKa = 4.19RR292 pKa = 11.84QCGMYY297 pKa = 11.08LMGSFYY303 pKa = 10.81AAQWPEE309 pKa = 3.75GTTVAEE315 pKa = 4.95DD316 pKa = 3.73GDD318 pKa = 4.3VYY320 pKa = 11.42AFNLPPIQEE329 pKa = 4.13EE330 pKa = 4.02HH331 pKa = 5.8GTPVLGGGEE340 pKa = 4.33FIGAFADD347 pKa = 4.07RR348 pKa = 11.84PEE350 pKa = 4.16VVAVQEE356 pKa = 4.08YY357 pKa = 10.58LSTVEE362 pKa = 4.15YY363 pKa = 10.63ANSRR367 pKa = 11.84ASLGSWFSAHH377 pKa = 7.35RR378 pKa = 11.84DD379 pKa = 3.28LDD381 pKa = 4.21LDD383 pKa = 4.1LLEE386 pKa = 5.47APTDD390 pKa = 3.58RR391 pKa = 11.84LGAEE395 pKa = 4.18TLRR398 pKa = 11.84DD399 pKa = 3.41SEE401 pKa = 4.46AVFRR405 pKa = 11.84FDD407 pKa = 4.91GSDD410 pKa = 3.28MMPASVGAGTFWRR423 pKa = 11.84GMTNWINGDD432 pKa = 3.51EE433 pKa = 4.38TEE435 pKa = 4.19EE436 pKa = 3.74TLAYY440 pKa = 9.59IEE442 pKa = 4.65EE443 pKa = 4.46SWPSSS448 pKa = 3.23

Molecular weight: 48.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1T4N2Y3|A0A1T4N2Y3_9ACTN Glutaredoxin OS=Marinactinospora thermotolerans DSM 45154 OX=1122192 GN=SAMN02745673_01245 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.99KK16 pKa = 9.77HH17 pKa = 5.81RR18 pKa = 11.84KK19 pKa = 8.55LLKK22 pKa = 7.77KK23 pKa = 9.13TRR25 pKa = 11.84IARR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.8KK32 pKa = 9.86

Molecular weight: 3.97 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4956

0

4956

1596438

29

12976

322.1

34.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.249 ± 0.05

0.75 ± 0.011

5.993 ± 0.036

6.375 ± 0.037

2.737 ± 0.021

9.527 ± 0.034

2.281 ± 0.015

3.46 ± 0.026

1.515 ± 0.021

10.442 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.776 ± 0.015

1.628 ± 0.017

6.279 ± 0.034

2.404 ± 0.023

8.803 ± 0.042

4.96 ± 0.027

5.661 ± 0.031

8.746 ± 0.041

1.462 ± 0.016

1.951 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski