Pseudomonas phage phiPsa374

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Otagovirus; Pseudomonas virus Psa374

Average proteome isoelectric point is 6.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 173 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W8EA10|W8EA10_9CAUD Uncharacterized protein OS=Pseudomonas phage phiPsa374 OX=1458843 PE=4 SV=1
MM1 pKa = 7.06TKK3 pKa = 10.11QYY5 pKa = 11.46VSFTTEE11 pKa = 3.42NARR14 pKa = 11.84VEE16 pKa = 4.11LDD18 pKa = 3.32GGVLLLQVTRR28 pKa = 11.84SYY30 pKa = 11.24GYY32 pKa = 10.21EE33 pKa = 4.01GEE35 pKa = 4.19QLSSYY40 pKa = 10.61DD41 pKa = 3.44HH42 pKa = 6.72YY43 pKa = 11.36DD44 pKa = 3.24IEE46 pKa = 4.54GLEE49 pKa = 4.3GEE51 pKa = 5.36PIAQLAQLAKK61 pKa = 10.43ALKK64 pKa = 8.89NLPEE68 pKa = 4.32EE69 pKa = 4.79GVFDD73 pKa = 3.97MDD75 pKa = 5.03QEE77 pKa = 4.23ALQDD81 pKa = 5.48FLMEE85 pKa = 4.33WAGDD89 pKa = 3.72TAEE92 pKa = 4.11MAEE95 pKa = 4.52LPFTFTDD102 pKa = 3.18RR103 pKa = 11.84FGDD106 pKa = 3.73VQTYY110 pKa = 8.48TPASMWEE117 pKa = 3.85ASGGCEE123 pKa = 3.9WEE125 pKa = 4.47EE126 pKa = 3.88SAQYY130 pKa = 10.98GSDD133 pKa = 3.63YY134 pKa = 10.59GWNVV138 pKa = 2.71

Molecular weight: 15.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W8EDN2|W8EDN2_9CAUD Uncharacterized protein OS=Pseudomonas phage phiPsa374 OX=1458843 PE=4 SV=1
MM1 pKa = 7.34RR2 pKa = 11.84TIKK5 pKa = 10.47AQEE8 pKa = 3.69FAKK11 pKa = 10.29PYY13 pKa = 10.16QYY15 pKa = 11.12EE16 pKa = 4.03QAKK19 pKa = 9.24KK20 pKa = 7.91QARR23 pKa = 11.84RR24 pKa = 11.84SDD26 pKa = 2.71IGRR29 pKa = 11.84RR30 pKa = 11.84SGRR33 pKa = 11.84RR34 pKa = 11.84GSQAWGVWAEE44 pKa = 4.19KK45 pKa = 10.23EE46 pKa = 4.04

Molecular weight: 5.44 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

173

0

173

28954

32

825

167.4

18.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.838 ± 0.237

1.281 ± 0.084

6.148 ± 0.197

6.493 ± 0.248

3.868 ± 0.123

7.281 ± 0.233

1.948 ± 0.123

5.716 ± 0.137

6.037 ± 0.245

7.944 ± 0.197

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.063 ± 0.097

4.638 ± 0.197

3.606 ± 0.127

3.986 ± 0.122

4.697 ± 0.151

5.54 ± 0.255

6.531 ± 0.263

7.153 ± 0.183

1.454 ± 0.086

3.778 ± 0.159
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski