Streptomyces phage Kromp

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 95 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A386K8I2|A0A386K8I2_9CAUD Uncharacterized protein OS=Streptomyces phage Kromp OX=2315619 GN=30 PE=4 SV=1
MM1 pKa = 7.03TASLTPAPANTDD13 pKa = 3.05QGQDD17 pKa = 3.51DD18 pKa = 4.68LVHH21 pKa = 6.03VVCFCDD27 pKa = 4.96QDD29 pKa = 3.77TALCGTNVTGYY40 pKa = 10.53DD41 pKa = 3.34WPDD44 pKa = 3.81DD45 pKa = 3.84EE46 pKa = 7.16ASTCVVCRR54 pKa = 11.84DD55 pKa = 4.6LEE57 pKa = 4.37HH58 pKa = 7.01KK59 pKa = 10.06PCNRR63 pKa = 11.84CGAA66 pKa = 3.91

Molecular weight:
7.08 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A386K8R8|A0A386K8R8_9CAUD Uncharacterized protein OS=Streptomyces phage Kromp OX=2315619 GN=24 PE=4 SV=1
MM1 pKa = 6.83TAHH4 pKa = 7.95PIPEE8 pKa = 4.38NAGHH12 pKa = 6.53WWLVSGKK19 pKa = 7.03WRR21 pKa = 11.84RR22 pKa = 11.84LHH24 pKa = 6.43AVPGEE29 pKa = 4.07AVTPDD34 pKa = 3.33QMRR37 pKa = 11.84ASTDD41 pKa = 3.11EE42 pKa = 3.9ARR44 pKa = 11.84PISGRR49 pKa = 11.84AACGMRR55 pKa = 11.84RR56 pKa = 11.84RR57 pKa = 11.84WWMPGLFSRR66 pKa = 11.84LGRR69 pKa = 11.84RR70 pKa = 11.84RR71 pKa = 11.84CTPCCTALGIPPGYY85 pKa = 8.12GTPANEE91 pKa = 3.76NDD93 pKa = 4.0LNRR96 pKa = 11.84KK97 pKa = 8.27DD98 pKa = 3.5QHH100 pKa = 5.13QQ101 pKa = 3.3

Molecular weight:
11.37 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

95

0

95

18218

34

1604

191.8

20.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.031 ± 0.475

1.059 ± 0.143

6.23 ± 0.239

5.604 ± 0.277

1.932 ± 0.148

8.53 ± 0.397

2.168 ± 0.199

3.54 ± 0.195

2.388 ± 0.252

8.821 ± 0.32

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.762 ± 0.1

1.784 ± 0.172

6.834 ± 0.346

3.914 ± 0.225

8.497 ± 0.386

4.99 ± 0.203

7.443 ± 0.344

7.377 ± 0.225

2.08 ± 0.137

2.014 ± 0.142

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski