Streptomyces phage Kromp
Average proteome isoelectric point is 6.75
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 95 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A386K8I2|A0A386K8I2_9CAUD Uncharacterized protein OS=Streptomyces phage Kromp OX=2315619 GN=30 PE=4 SV=1
MM1 pKa = 7.03 TASLTPAPANTDD13 pKa = 3.05 QGQDD17 pKa = 3.51 DD18 pKa = 4.68 LVHH21 pKa = 6.03 VVCFCDD27 pKa = 4.96 QDD29 pKa = 3.77 TALCGTNVTGYY40 pKa = 10.53 DD41 pKa = 3.34 WPDD44 pKa = 3.81 DD45 pKa = 3.84 EE46 pKa = 7.16 ASTCVVCRR54 pKa = 11.84 DD55 pKa = 4.6 LEE57 pKa = 4.37 HH58 pKa = 7.01 KK59 pKa = 10.06 PCNRR63 pKa = 11.84 CGAA66 pKa = 3.91
Molecular weight: 7.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.902
IPC2_protein 3.961
IPC_protein 3.884
Toseland 3.668
ProMoST 4.101
Dawson 3.91
Bjellqvist 4.075
Wikipedia 3.91
Rodwell 3.719
Grimsley 3.592
Solomon 3.897
Lehninger 3.846
Nozaki 4.05
DTASelect 4.329
Thurlkill 3.757
EMBOSS 3.91
Sillero 4.024
Patrickios 0.172
IPC_peptide 3.884
IPC2_peptide 3.986
IPC2.peptide.svr19 3.917
Protein with the highest isoelectric point:
>tr|A0A386K8R8|A0A386K8R8_9CAUD Uncharacterized protein OS=Streptomyces phage Kromp OX=2315619 GN=24 PE=4 SV=1
MM1 pKa = 6.83 TAHH4 pKa = 7.95 PIPEE8 pKa = 4.38 NAGHH12 pKa = 6.53 WWLVSGKK19 pKa = 7.03 WRR21 pKa = 11.84 RR22 pKa = 11.84 LHH24 pKa = 6.43 AVPGEE29 pKa = 4.07 AVTPDD34 pKa = 3.33 QMRR37 pKa = 11.84 ASTDD41 pKa = 3.11 EE42 pKa = 3.9 ARR44 pKa = 11.84 PISGRR49 pKa = 11.84 AACGMRR55 pKa = 11.84 RR56 pKa = 11.84 RR57 pKa = 11.84 WWMPGLFSRR66 pKa = 11.84 LGRR69 pKa = 11.84 RR70 pKa = 11.84 RR71 pKa = 11.84 CTPCCTALGIPPGYY85 pKa = 8.12 GTPANEE91 pKa = 3.76 NDD93 pKa = 4.0 LNRR96 pKa = 11.84 KK97 pKa = 8.27 DD98 pKa = 3.5 QHH100 pKa = 5.13 QQ101 pKa = 3.3
Molecular weight: 11.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.336
IPC2_protein 9.224
IPC_protein 10.014
Toseland 10.35
ProMoST 10.116
Dawson 10.467
Bjellqvist 10.277
Wikipedia 10.687
Rodwell 10.482
Grimsley 10.511
Solomon 10.628
Lehninger 10.613
Nozaki 10.482
DTASelect 10.218
Thurlkill 10.379
EMBOSS 10.774
Sillero 10.452
Patrickios 10.35
IPC_peptide 10.643
IPC2_peptide 9.882
IPC2.peptide.svr19 8.333
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
95
0
95
18218
34
1604
191.8
20.75
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.031 ± 0.475
1.059 ± 0.143
6.23 ± 0.239
5.604 ± 0.277
1.932 ± 0.148
8.53 ± 0.397
2.168 ± 0.199
3.54 ± 0.195
2.388 ± 0.252
8.821 ± 0.32
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.762 ± 0.1
1.784 ± 0.172
6.834 ± 0.346
3.914 ± 0.225
8.497 ± 0.386
4.99 ± 0.203
7.443 ± 0.344
7.377 ± 0.225
2.08 ± 0.137
2.014 ± 0.142
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here