Dissoconium aciculare CBS 342.82
Average proteome isoelectric point is 6.75
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 10290 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6J3M3Q1|A0A6J3M3Q1_9PEZI uncharacterized protein OS=Dissoconium aciculare CBS 342.82 OX=1314786 GN=K489DRAFT_380031 PE=4 SV=1
MM1 pKa = 7.6 QITQVLSASVVLFAYY16 pKa = 10.08 SALSAPTVGLIGIHH30 pKa = 5.63 EE31 pKa = 4.58 RR32 pKa = 11.84 EE33 pKa = 4.19 ASDD36 pKa = 4.71 ADD38 pKa = 3.54 SAYY41 pKa = 10.07 TNFARR46 pKa = 11.84 SDD48 pKa = 3.01 ADD50 pKa = 3.23 AAYY53 pKa = 9.6 TNFARR58 pKa = 11.84 SDD60 pKa = 3.01 ADD62 pKa = 3.23 AAYY65 pKa = 9.6 TNFARR70 pKa = 11.84 SDD72 pKa = 3.4 ADD74 pKa = 3.46 VAYY77 pKa = 10.93 SNAARR82 pKa = 11.84 SDD84 pKa = 3.35 ADD86 pKa = 3.49 VAYY89 pKa = 10.93 SNAARR94 pKa = 11.84 SDD96 pKa = 3.35 ADD98 pKa = 3.49 VAYY101 pKa = 10.93 SNAARR106 pKa = 11.84 SDD108 pKa = 3.35 ADD110 pKa = 3.49 VAYY113 pKa = 10.93 SNAARR118 pKa = 11.84 SDD120 pKa = 3.28 ADD122 pKa = 3.6 TAYY125 pKa = 11.33 SNAARR130 pKa = 11.84 SDD132 pKa = 3.28 ADD134 pKa = 3.6 TAYY137 pKa = 11.33 SNAARR142 pKa = 11.84 SDD144 pKa = 3.04 ADD146 pKa = 3.24 AAYY149 pKa = 9.78 TNFARR154 pKa = 11.84 EE155 pKa = 4.03 ASDD158 pKa = 4.94 ADD160 pKa = 3.22 IAYY163 pKa = 10.85 SNAAHH168 pKa = 6.94 AA169 pKa = 4.81
Molecular weight: 17.54 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.759
IPC2_protein 3.948
IPC_protein 3.973
Toseland 3.732
ProMoST 4.164
Dawson 3.986
Bjellqvist 4.139
Wikipedia 3.973
Rodwell 3.795
Grimsley 3.63
Solomon 3.986
Lehninger 3.935
Nozaki 4.101
DTASelect 4.431
Thurlkill 3.795
EMBOSS 3.973
Sillero 4.101
Patrickios 1.964
IPC_peptide 3.973
IPC2_peptide 4.075
IPC2.peptide.svr19 3.984
Protein with the highest isoelectric point:
>tr|A0A6J3M5J3|A0A6J3M5J3_9PEZI Protein YIP OS=Dissoconium aciculare CBS 342.82 OX=1314786 GN=K489DRAFT_379318 PE=3 SV=1
MM1 pKa = 7.88 PSHH4 pKa = 6.91 KK5 pKa = 10.39 SFRR8 pKa = 11.84 TKK10 pKa = 10.45 QKK12 pKa = 9.84 LAKK15 pKa = 9.55 AQKK18 pKa = 8.59 QNRR21 pKa = 11.84 PIPQWIRR28 pKa = 11.84 LRR30 pKa = 11.84 TGNTIRR36 pKa = 11.84 YY37 pKa = 5.79 NAKK40 pKa = 8.89 RR41 pKa = 11.84 RR42 pKa = 11.84 HH43 pKa = 4.15 WRR45 pKa = 11.84 KK46 pKa = 7.38 TRR48 pKa = 11.84 IGII51 pKa = 4.0
Molecular weight: 6.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.39
IPC2_protein 11.052
IPC_protein 12.398
Toseland 12.559
ProMoST 13.042
Dawson 12.559
Bjellqvist 12.544
Wikipedia 13.027
Rodwell 12.34
Grimsley 12.603
Solomon 13.056
Lehninger 12.954
Nozaki 12.559
DTASelect 12.544
Thurlkill 12.559
EMBOSS 13.056
Sillero 12.559
Patrickios 12.076
IPC_peptide 13.056
IPC2_peptide 12.032
IPC2.peptide.svr19 9.006
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
10289
1
10290
4500263
50
4912
437.3
48.31
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.273 ± 0.025
1.292 ± 0.01
5.769 ± 0.018
5.909 ± 0.024
3.585 ± 0.014
6.688 ± 0.022
2.555 ± 0.011
4.827 ± 0.016
4.558 ± 0.022
8.841 ± 0.029
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.097 ± 0.008
3.61 ± 0.013
5.869 ± 0.024
4.169 ± 0.022
6.527 ± 0.022
8.377 ± 0.029
6.057 ± 0.019
5.995 ± 0.016
1.389 ± 0.01
2.61 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here