Devosia sp. LC5
Average proteome isoelectric point is 6.31
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4116 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A085F4N9|A0A085F4N9_9RHIZ ABC transporter membrane spanning protein (Sugar) OS=Devosia sp. LC5 OX=1502724 GN=FF80_02697 PE=3 SV=1
MM1 pKa = 7.89 RR2 pKa = 11.84 IIALTTIFAALSIAPVLADD21 pKa = 4.79 DD22 pKa = 5.4 VPANLNGHH30 pKa = 5.66 WAIDD34 pKa = 4.15 GACDD38 pKa = 3.36 NADD41 pKa = 3.35 DD42 pKa = 4.38 SVHH45 pKa = 7.55 IEE47 pKa = 3.99 GNMLALGTGEE57 pKa = 3.84 PLAVVFYY64 pKa = 10.32 PDD66 pKa = 5.21 DD67 pKa = 4.2 SPSGNGAIHH76 pKa = 6.4 WEE78 pKa = 4.29 EE79 pKa = 4.28 EE80 pKa = 4.27 GSVDD84 pKa = 3.49 NFEE87 pKa = 4.6 YY88 pKa = 11.23 VPDD91 pKa = 4.04 SDD93 pKa = 3.81 EE94 pKa = 5.79 LIYY97 pKa = 11.17 NPEE100 pKa = 4.23 GYY102 pKa = 11.33 GMGDD106 pKa = 4.02 AKK108 pKa = 10.72 PSLYY112 pKa = 10.19 KK113 pKa = 10.18 RR114 pKa = 11.84 CKK116 pKa = 9.59 SS117 pKa = 3.25
Molecular weight: 12.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.751
IPC2_protein 3.935
IPC_protein 3.897
Toseland 3.694
ProMoST 4.062
Dawson 3.884
Bjellqvist 4.05
Wikipedia 3.821
Rodwell 3.732
Grimsley 3.605
Solomon 3.872
Lehninger 3.834
Nozaki 4.012
DTASelect 4.228
Thurlkill 3.745
EMBOSS 3.834
Sillero 4.024
Patrickios 0.947
IPC_peptide 3.872
IPC2_peptide 3.999
IPC2.peptide.svr19 3.903
Protein with the highest isoelectric point:
>tr|A0A085FCD0|A0A085FCD0_9RHIZ Gfa-like protein OS=Devosia sp. LC5 OX=1502724 GN=FF80_01393 PE=3 SV=1
MM1 pKa = 7.26 SQPKK5 pKa = 9.63 KK6 pKa = 9.95 PPRR9 pKa = 11.84 VTHH12 pKa = 6.65 ALAARR17 pKa = 11.84 HH18 pKa = 5.04 EE19 pKa = 4.27 ALWLRR24 pKa = 11.84 LTALHH29 pKa = 6.11 AQIAAVAARR38 pKa = 11.84 RR39 pKa = 11.84 AQAPVSGQTISVAEE53 pKa = 3.97 ALLRR57 pKa = 11.84 DD58 pKa = 3.72 AQVFLGLGDD67 pKa = 4.48 VLPMAAPDD75 pKa = 3.57 HH76 pKa = 6.59 GGLLTQLGQALAGLDD91 pKa = 2.89 AWEE94 pKa = 4.49 AVNAEE99 pKa = 3.63 WRR101 pKa = 11.84 GEE103 pKa = 3.85 LNAFVWKK110 pKa = 10.71 VVGDD114 pKa = 3.68 PVLPVQRR121 pKa = 11.84 LRR123 pKa = 11.84 PKK125 pKa = 10.22 LVAAAAPHH133 pKa = 7.04 ADD135 pKa = 3.63 PAYY138 pKa = 9.61 AAYY141 pKa = 8.8 KK142 pKa = 10.74 ARR144 pKa = 11.84 MADD147 pKa = 3.36 LRR149 pKa = 11.84 QNLPSASSNSTIARR163 pKa = 11.84 PNRR166 pKa = 11.84 HH167 pKa = 6.35 SGFEE171 pKa = 4.2 LPPSRR176 pKa = 11.84 AGRR179 pKa = 11.84 RR180 pKa = 11.84 LGAPSRR186 pKa = 11.84 AARR189 pKa = 11.84 EE190 pKa = 3.97 PLCWQATRR198 pKa = 11.84 SGYY201 pKa = 10.47 FDD203 pKa = 5.74 DD204 pKa = 4.68 PACRR208 pKa = 11.84 FHH210 pKa = 7.37 PPPAA214 pKa = 5.32
Molecular weight: 22.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.346
IPC2_protein 9.458
IPC_protein 10.335
Toseland 10.526
ProMoST 10.262
Dawson 10.628
Bjellqvist 10.379
Wikipedia 10.862
Rodwell 10.716
Grimsley 10.687
Solomon 10.76
Lehninger 10.73
Nozaki 10.526
DTASelect 10.365
Thurlkill 10.526
EMBOSS 10.935
Sillero 10.57
Patrickios 10.452
IPC_peptide 10.774
IPC2_peptide 9.487
IPC2.peptide.svr19 8.647
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4116
0
4116
1240715
30
1832
301.4
32.61
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.516 ± 0.046
0.669 ± 0.012
5.651 ± 0.032
5.454 ± 0.032
3.855 ± 0.025
8.591 ± 0.037
1.979 ± 0.02
5.66 ± 0.026
3.134 ± 0.03
10.289 ± 0.04
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.555 ± 0.016
2.813 ± 0.022
5.126 ± 0.03
3.299 ± 0.023
6.372 ± 0.04
5.441 ± 0.026
5.517 ± 0.026
7.462 ± 0.036
1.327 ± 0.015
2.29 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here