Lactobacillus rennini DSM 20253
Average proteome isoelectric point is 7.38
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2191 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0R2CYB2|A0A0R2CYB2_9LACO Uncharacterized protein OS=Lactobacillus rennini DSM 20253 OX=1423796 GN=FC24_GL000242 PE=4 SV=1
MM1 pKa = 7.8 KK2 pKa = 10.39 YY3 pKa = 10.71 AEE5 pKa = 4.25 LKK7 pKa = 10.66 KK8 pKa = 10.57 LVEE11 pKa = 4.28 ATEE14 pKa = 3.81 EE15 pKa = 3.69 NYY17 pKa = 10.99 YY18 pKa = 10.15 YY19 pKa = 10.96 VGVRR23 pKa = 11.84 FEE25 pKa = 4.11 DD26 pKa = 3.56 RR27 pKa = 11.84 EE28 pKa = 4.22 YY29 pKa = 11.53 NDD31 pKa = 3.8 GDD33 pKa = 3.34 IVAYY37 pKa = 10.71 SKK39 pKa = 11.11 DD40 pKa = 3.49 NPDD43 pKa = 3.53 RR44 pKa = 11.84 QDD46 pKa = 3.13 EE47 pKa = 4.28 RR48 pKa = 11.84 DD49 pKa = 3.73 FPEE52 pKa = 4.91 FGTPEE57 pKa = 4.1 YY58 pKa = 11.03 DD59 pKa = 5.32 DD60 pKa = 5.83 LPEE63 pKa = 6.09 LDD65 pKa = 5.75 GSSAWYY71 pKa = 9.7 IDD73 pKa = 3.81 APTMLNFDD81 pKa = 3.72 TSIYY85 pKa = 10.23 IPDD88 pKa = 3.79 HH89 pKa = 6.61 AYY91 pKa = 10.7 VIASNEE97 pKa = 3.77 MGGDD101 pKa = 3.36 DD102 pKa = 5.09 NYY104 pKa = 11.66 AVDD107 pKa = 3.92 YY108 pKa = 11.44 GEE110 pKa = 5.24 ILIKK114 pKa = 10.54 DD115 pKa = 4.01 AIVIKK120 pKa = 10.54 KK121 pKa = 9.84 LWW123 pKa = 3.43
Molecular weight: 14.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.768
IPC2_protein 3.948
IPC_protein 3.935
Toseland 3.719
ProMoST 4.088
Dawson 3.91
Bjellqvist 4.075
Wikipedia 3.846
Rodwell 3.757
Grimsley 3.63
Solomon 3.91
Lehninger 3.859
Nozaki 4.024
DTASelect 4.253
Thurlkill 3.77
EMBOSS 3.846
Sillero 4.05
Patrickios 1.914
IPC_peptide 3.91
IPC2_peptide 4.024
IPC2.peptide.svr19 3.942
Protein with the highest isoelectric point:
>tr|A0A0R2CSU2|A0A0R2CSU2_9LACO General stress protein A OS=Lactobacillus rennini DSM 20253 OX=1423796 GN=FC24_GL000110 PE=4 SV=1
MM1 pKa = 7.43 AMKK4 pKa = 9.57 RR5 pKa = 11.84 TYY7 pKa = 9.69 QPKK10 pKa = 8.69 KK11 pKa = 7.77 RR12 pKa = 11.84 HH13 pKa = 4.99 RR14 pKa = 11.84 QRR16 pKa = 11.84 VHH18 pKa = 7.37 GFMKK22 pKa = 10.61 RR23 pKa = 11.84 MNTKK27 pKa = 9.67 NGRR30 pKa = 11.84 NVLQRR35 pKa = 11.84 RR36 pKa = 11.84 RR37 pKa = 11.84 RR38 pKa = 11.84 KK39 pKa = 8.93 GRR41 pKa = 11.84 KK42 pKa = 8.7 VLSAA46 pKa = 4.05
Molecular weight: 5.69 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.433
IPC2_protein 11.125
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.398
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.135
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.06
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2191
0
2191
652553
46
1452
297.8
33.14
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.332 ± 0.059
0.562 ± 0.015
5.147 ± 0.043
4.496 ± 0.048
4.171 ± 0.041
6.597 ± 0.052
2.333 ± 0.024
6.724 ± 0.049
6.284 ± 0.055
10.509 ± 0.07
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.505 ± 0.025
4.324 ± 0.036
3.898 ± 0.026
5.904 ± 0.068
4.29 ± 0.04
5.147 ± 0.042
6.348 ± 0.04
6.912 ± 0.044
1.009 ± 0.022
3.491 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here