Amino acid dipepetide frequency for Streptococcus sp. DD12

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.368AlaAla: 6.368 ± 0.151
0.603AlaCys: 0.603 ± 0.038
5.159AlaAsp: 5.159 ± 0.134
4.885AlaGlu: 4.885 ± 0.129
3.972AlaPhe: 3.972 ± 0.11
6.056AlaGly: 6.056 ± 0.142
1.581AlaHis: 1.581 ± 0.058
6.553AlaIle: 6.553 ± 0.13
5.589AlaLys: 5.589 ± 0.114
9.495AlaLeu: 9.495 ± 0.173
2.231AlaMet: 2.231 ± 0.059
3.391AlaAsn: 3.391 ± 0.087
2.615AlaPro: 2.615 ± 0.076
4.741AlaGln: 4.741 ± 0.13
3.305AlaArg: 3.305 ± 0.099
6.245AlaSer: 6.245 ± 0.289
5.056AlaThr: 5.056 ± 0.129
6.424AlaVal: 6.424 ± 0.13
0.78AlaTrp: 0.78 ± 0.037
3.391AlaTyr: 3.391 ± 0.094
0.0AlaXaa: 0.0 ± 0.0
Cys
0.328CysAla: 0.328 ± 0.023
0.042CysCys: 0.042 ± 0.009
0.352CysAsp: 0.352 ± 0.026
0.195CysGlu: 0.195 ± 0.021
0.247CysPhe: 0.247 ± 0.021
0.449CysGly: 0.449 ± 0.032
0.183CysHis: 0.183 ± 0.02
0.233CysIle: 0.233 ± 0.024
0.139CysLys: 0.139 ± 0.018
0.567CysLeu: 0.567 ± 0.036
0.097CysMet: 0.097 ± 0.014
0.157CysAsn: 0.157 ± 0.017
0.257CysPro: 0.257 ± 0.025
0.324CysGln: 0.324 ± 0.026
0.241CysArg: 0.241 ± 0.024
0.272CysSer: 0.272 ± 0.025
0.189CysThr: 0.189 ± 0.018
0.34CysVal: 0.34 ± 0.031
0.048CysTrp: 0.048 ± 0.009
0.211CysTyr: 0.211 ± 0.02
0.0CysXaa: 0.0 ± 0.0
Asp
4.501AspAla: 4.501 ± 0.108
0.259AspCys: 0.259 ± 0.026
3.003AspAsp: 3.003 ± 0.087
3.626AspGlu: 3.626 ± 0.098
3.21AspPhe: 3.21 ± 0.084
4.147AspGly: 4.147 ± 0.138
1.102AspHis: 1.102 ± 0.048
3.958AspIle: 3.958 ± 0.113
3.896AspLys: 3.896 ± 0.103
6.307AspLeu: 6.307 ± 0.139
1.579AspMet: 1.579 ± 0.063
2.114AspAsn: 2.114 ± 0.072
1.858AspPro: 1.858 ± 0.063
2.733AspGln: 2.733 ± 0.081
2.488AspArg: 2.488 ± 0.074
3.264AspSer: 3.264 ± 0.089
2.876AspThr: 2.876 ± 0.093
4.017AspVal: 4.017 ± 0.11
0.78AspTrp: 0.78 ± 0.041
2.906AspTyr: 2.906 ± 0.077
0.0AspXaa: 0.0 ± 0.0
Glu
6.533GluAla: 6.533 ± 0.135
0.175GluCys: 0.175 ± 0.022
3.602GluAsp: 3.602 ± 0.103
4.781GluGlu: 4.781 ± 0.138
2.019GluPhe: 2.019 ± 0.068
3.642GluGly: 3.642 ± 0.092
1.086GluHis: 1.086 ± 0.053
3.914GluIle: 3.914 ± 0.115
4.483GluLys: 4.483 ± 0.106
5.613GluLeu: 5.613 ± 0.124
1.591GluMet: 1.591 ± 0.059
2.888GluAsn: 2.888 ± 0.085
1.557GluPro: 1.557 ± 0.063
2.562GluGln: 2.562 ± 0.081
3.25GluArg: 3.25 ± 0.094
3.918GluSer: 3.918 ± 0.539
3.731GluThr: 3.731 ± 0.085
4.495GluVal: 4.495 ± 0.107
0.491GluTrp: 0.491 ± 0.027
1.325GluTyr: 1.325 ± 0.057
0.0GluXaa: 0.0 ± 0.0
Phe
3.52PheAla: 3.52 ± 0.101
0.243PheCys: 0.243 ± 0.022
3.055PheAsp: 3.055 ± 0.09
2.687PheGlu: 2.687 ± 0.08
1.959PhePhe: 1.959 ± 0.085
3.337PheGly: 3.337 ± 0.08
0.764PheHis: 0.764 ± 0.041
2.721PheIle: 2.721 ± 0.096
2.152PheLys: 2.152 ± 0.076
4.236PheLeu: 4.236 ± 0.14
0.969PheMet: 0.969 ± 0.044
1.623PheAsn: 1.623 ± 0.061
1.607PhePro: 1.607 ± 0.063
1.579PheGln: 1.579 ± 0.059
1.498PheArg: 1.498 ± 0.055
3.158PheSer: 3.158 ± 0.084
2.325PheThr: 2.325 ± 0.063
3.099PheVal: 3.099 ± 0.094
0.525PheTrp: 0.525 ± 0.039
1.7PheTyr: 1.7 ± 0.067
0.0PheXaa: 0.0 ± 0.0
Gly
5.259GlyAla: 5.259 ± 0.139
0.386GlyCys: 0.386 ± 0.028
3.781GlyAsp: 3.781 ± 0.095
3.54GlyGlu: 3.54 ± 0.084
3.345GlyPhe: 3.345 ± 0.086
4.469GlyGly: 4.469 ± 0.125
1.577GlyHis: 1.577 ± 0.059
4.885GlyIle: 4.885 ± 0.132
4.2GlyLys: 4.2 ± 0.108
7.693GlyLeu: 7.693 ± 0.144
1.824GlyMet: 1.824 ± 0.064
2.657GlyAsn: 2.657 ± 0.071
1.675GlyPro: 1.675 ± 0.06
3.916GlyGln: 3.916 ± 0.108
3.148GlyArg: 3.148 ± 0.076
3.743GlySer: 3.743 ± 0.099
3.846GlyThr: 3.846 ± 0.108
5.064GlyVal: 5.064 ± 0.116
0.638GlyTrp: 0.638 ± 0.035
2.693GlyTyr: 2.693 ± 0.081
0.0GlyXaa: 0.0 ± 0.0
His
1.309HisAla: 1.309 ± 0.056
0.133HisCys: 0.133 ± 0.018
0.937HisAsp: 0.937 ± 0.045
1.052HisGlu: 1.052 ± 0.041
1.05HisPhe: 1.05 ± 0.049
1.267HisGly: 1.267 ± 0.057
0.515HisHis: 0.515 ± 0.036
1.152HisIle: 1.152 ± 0.051
0.879HisLys: 0.879 ± 0.04
2.295HisLeu: 2.295 ± 0.082
0.501HisMet: 0.501 ± 0.032
0.619HisAsn: 0.619 ± 0.036
1.054HisPro: 1.054 ± 0.045
1.072HisGln: 1.072 ± 0.053
1.024HisArg: 1.024 ± 0.046
0.901HisSer: 0.901 ± 0.038
0.913HisThr: 0.913 ± 0.042
1.229HisVal: 1.229 ± 0.048
0.241HisTrp: 0.241 ± 0.02
0.996HisTyr: 0.996 ± 0.046
0.0HisXaa: 0.0 ± 0.0
Ile
6.271IleAla: 6.271 ± 0.148
0.455IleCys: 0.455 ± 0.032
3.952IleAsp: 3.952 ± 0.103
3.648IleGlu: 3.648 ± 0.103
2.703IlePhe: 2.703 ± 0.094
4.7IleGly: 4.7 ± 0.121
1.152IleHis: 1.152 ± 0.054
3.932IleIle: 3.932 ± 0.117
3.124IleLys: 3.124 ± 0.087
6.295IleLeu: 6.295 ± 0.163
1.335IleMet: 1.335 ± 0.055
2.442IleAsn: 2.442 ± 0.071
2.54IlePro: 2.54 ± 0.068
2.428IleGln: 2.428 ± 0.068
2.681IleArg: 2.681 ± 0.084
4.198IleSer: 4.198 ± 0.106
3.763IleThr: 3.763 ± 0.079
4.425IleVal: 4.425 ± 0.103
0.559IleTrp: 0.559 ± 0.038
2.144IleTyr: 2.144 ± 0.066
0.0IleXaa: 0.0 ± 0.0
Lys
5.875LysAla: 5.875 ± 0.134
0.147LysCys: 0.147 ± 0.016
3.646LysAsp: 3.646 ± 0.087
4.883LysGlu: 4.883 ± 0.123
1.44LysPhe: 1.44 ± 0.062
3.836LysGly: 3.836 ± 0.091
1.008LysHis: 1.008 ± 0.051
3.232LysIle: 3.232 ± 0.085
4.191LysLys: 4.191 ± 0.133
4.481LysLeu: 4.481 ± 0.096
1.494LysMet: 1.494 ± 0.055
2.407LysAsn: 2.407 ± 0.072
1.931LysPro: 1.931 ± 0.066
2.395LysGln: 2.395 ± 0.079
3.105LysArg: 3.105 ± 0.091
3.101LysSer: 3.101 ± 0.08
4.002LysThr: 4.002 ± 0.095
3.797LysVal: 3.797 ± 0.11
0.489LysTrp: 0.489 ± 0.03
1.617LysTyr: 1.617 ± 0.057
0.0LysXaa: 0.0 ± 0.0
Leu
10.762LeuAla: 10.762 ± 0.183
0.489LeuCys: 0.489 ± 0.034
5.961LeuAsp: 5.961 ± 0.116
6.293LeuGlu: 6.293 ± 0.14
4.222LeuPhe: 4.222 ± 0.149
6.887LeuGly: 6.887 ± 0.162
1.593LeuHis: 1.593 ± 0.059
5.931LeuIle: 5.931 ± 0.167
5.525LeuLys: 5.525 ± 0.123
10.465LeuLeu: 10.465 ± 0.234
2.377LeuMet: 2.377 ± 0.067
3.508LeuAsn: 3.508 ± 0.101
4.459LeuPro: 4.459 ± 0.088
3.749LeuGln: 3.749 ± 0.104
4.002LeuArg: 4.002 ± 0.115
8.757LeuSer: 8.757 ± 0.882
6.893LeuThr: 6.893 ± 0.122
7.802LeuVal: 7.802 ± 0.141
0.815LeuTrp: 0.815 ± 0.042
3.144LeuTyr: 3.144 ± 0.081
0.0LeuXaa: 0.0 ± 0.0
Met
2.58MetAla: 2.58 ± 0.078
0.105MetCys: 0.105 ± 0.016
1.279MetAsp: 1.279 ± 0.053
1.233MetGlu: 1.233 ± 0.046
0.708MetPhe: 0.708 ± 0.037
1.726MetGly: 1.726 ± 0.061
0.366MetHis: 0.366 ± 0.025
1.438MetIle: 1.438 ± 0.057
1.372MetLys: 1.372 ± 0.055
2.003MetLeu: 2.003 ± 0.073
0.636MetMet: 0.636 ± 0.039
0.909MetAsn: 0.909 ± 0.048
0.879MetPro: 0.879 ± 0.047
0.971MetGln: 0.971 ± 0.046
1.042MetArg: 1.042 ± 0.051
1.669MetSer: 1.669 ± 0.13
2.226MetThr: 2.226 ± 0.072
1.722MetVal: 1.722 ± 0.061
0.155MetTrp: 0.155 ± 0.019
0.489MetTyr: 0.489 ± 0.031
0.0MetXaa: 0.0 ± 0.0
Asn
3.154AsnAla: 3.154 ± 0.08
0.197AsnCys: 0.197 ± 0.023
2.043AsnAsp: 2.043 ± 0.071
1.762AsnGlu: 1.762 ± 0.068
1.573AsnPhe: 1.573 ± 0.051
3.033AsnGly: 3.033 ± 0.09
0.891AsnHis: 0.891 ± 0.046
2.351AsnIle: 2.351 ± 0.077
1.911AsnLys: 1.911 ± 0.073
3.797AsnLeu: 3.797 ± 0.095
0.909AsnMet: 0.909 ± 0.047
1.434AsnAsn: 1.434 ± 0.062
1.961AsnPro: 1.961 ± 0.071
2.15AsnGln: 2.15 ± 0.077
1.997AsnArg: 1.997 ± 0.069
2.146AsnSer: 2.146 ± 0.085
2.088AsnThr: 2.088 ± 0.066
2.329AsnVal: 2.329 ± 0.068
0.537AsnTrp: 0.537 ± 0.035
1.617AsnTyr: 1.617 ± 0.059
0.0AsnXaa: 0.0 ± 0.0
Pro
2.828ProAla: 2.828 ± 0.074
0.153ProCys: 0.153 ± 0.017
2.315ProAsp: 2.315 ± 0.076
2.886ProGlu: 2.886 ± 0.09
1.637ProPhe: 1.637 ± 0.058
2.106ProGly: 2.106 ± 0.074
0.764ProHis: 0.764 ± 0.042
2.22ProIle: 2.22 ± 0.069
2.052ProLys: 2.052 ± 0.061
3.566ProLeu: 3.566 ± 0.081
0.674ProMet: 0.674 ± 0.037
1.398ProAsn: 1.398 ± 0.047
0.621ProPro: 0.621 ± 0.033
1.77ProGln: 1.77 ± 0.054
1.189ProArg: 1.189 ± 0.054
2.237ProSer: 2.237 ± 0.059
2.047ProThr: 2.047 ± 0.065
2.788ProVal: 2.788 ± 0.078
0.312ProTrp: 0.312 ± 0.025
1.406ProTyr: 1.406 ± 0.053
0.0ProXaa: 0.0 ± 0.0
Gln
5.807GlnAla: 5.807 ± 0.122
0.123GlnCys: 0.123 ± 0.017
2.516GlnAsp: 2.516 ± 0.068
3.693GlnGlu: 3.693 ± 0.091
1.573GlnPhe: 1.573 ± 0.054
2.965GlnGly: 2.965 ± 0.081
0.734GlnHis: 0.734 ± 0.044
2.518GlnIle: 2.518 ± 0.072
3.019GlnLys: 3.019 ± 0.096
4.737GlnLeu: 4.737 ± 0.111
1.086GlnMet: 1.086 ± 0.047
1.48GlnAsn: 1.48 ± 0.059
1.366GlnPro: 1.366 ± 0.055
1.834GlnGln: 1.834 ± 0.078
1.774GlnArg: 1.774 ± 0.06
2.89GlnSer: 2.89 ± 0.297
3.079GlnThr: 3.079 ± 0.093
4.107GlnVal: 4.107 ± 0.109
0.447GlnTrp: 0.447 ± 0.033
1.273GlnTyr: 1.273 ± 0.061
0.0GlnXaa: 0.0 ± 0.0
Arg
2.761ArgAla: 2.761 ± 0.091
0.167ArgCys: 0.167 ± 0.018
2.492ArgAsp: 2.492 ± 0.078
2.914ArgGlu: 2.914 ± 0.098
2.214ArgPhe: 2.214 ± 0.074
2.422ArgGly: 2.422 ± 0.07
1.028ArgHis: 1.028 ± 0.047
2.82ArgIle: 2.82 ± 0.074
2.498ArgLys: 2.498 ± 0.073
5.107ArgLeu: 5.107 ± 0.12
1.066ArgMet: 1.066 ± 0.054
1.51ArgAsn: 1.51 ± 0.059
1.653ArgPro: 1.653 ± 0.054
2.697ArgGln: 2.697 ± 0.074
2.186ArgArg: 2.186 ± 0.081
2.132ArgSer: 2.132 ± 0.07
1.959ArgThr: 1.959 ± 0.077
3.085ArgVal: 3.085 ± 0.092
0.368ArgTrp: 0.368 ± 0.027
1.911ArgTyr: 1.911 ± 0.061
0.0ArgXaa: 0.0 ± 0.0
Ser
4.833SerAla: 4.833 ± 0.236
0.267SerCys: 0.267 ± 0.026
3.733SerAsp: 3.733 ± 0.091
4.097SerGlu: 4.097 ± 0.539
2.834SerPhe: 2.834 ± 0.077
4.753SerGly: 4.753 ± 0.099
1.466SerHis: 1.466 ± 0.062
3.528SerIle: 3.528 ± 0.095
3.035SerLys: 3.035 ± 0.089
8.188SerLeu: 8.188 ± 0.895
1.426SerMet: 1.426 ± 0.127
2.357SerAsn: 2.357 ± 0.074
2.102SerPro: 2.102 ± 0.069
4.177SerGln: 4.177 ± 0.325
2.709SerArg: 2.709 ± 0.074
4.272SerSer: 4.272 ± 0.182
4.477SerThr: 4.477 ± 0.81
4.566SerVal: 4.566 ± 0.409
0.672SerTrp: 0.672 ± 0.038
2.578SerTyr: 2.578 ± 0.077
0.0SerXaa: 0.0 ± 0.0
Thr
5.247ThrAla: 5.247 ± 0.136
0.312ThrCys: 0.312 ± 0.03
3.618ThrAsp: 3.618 ± 0.114
3.079ThrGlu: 3.079 ± 0.1
2.655ThrPhe: 2.655 ± 0.079
4.704ThrGly: 4.704 ± 0.102
1.114ThrHis: 1.114 ± 0.046
4.22ThrIle: 4.22 ± 0.09
3.142ThrLys: 3.142 ± 0.092
6.074ThrLeu: 6.074 ± 0.114
1.12ThrMet: 1.12 ± 0.049
2.347ThrAsn: 2.347 ± 0.078
2.578ThrPro: 2.578 ± 0.085
2.552ThrGln: 2.552 ± 0.068
2.031ThrArg: 2.031 ± 0.068
5.103ThrSer: 5.103 ± 0.817
3.721ThrThr: 3.721 ± 0.126
4.708ThrVal: 4.708 ± 0.097
0.587ThrTrp: 0.587 ± 0.033
2.655ThrTyr: 2.655 ± 0.082
0.0ThrXaa: 0.0 ± 0.0
Val
6.887ValAla: 6.887 ± 0.137
0.37ValCys: 0.37 ± 0.03
4.232ValAsp: 4.232 ± 0.106
3.848ValGlu: 3.848 ± 0.092
3.035ValPhe: 3.035 ± 0.091
4.769ValGly: 4.769 ± 0.121
1.165ValHis: 1.165 ± 0.052
4.833ValIle: 4.833 ± 0.112
3.886ValLys: 3.886 ± 0.092
7.287ValLeu: 7.287 ± 0.166
1.681ValMet: 1.681 ± 0.063
2.753ValAsn: 2.753 ± 0.08
2.595ValPro: 2.595 ± 0.075
2.42ValGln: 2.42 ± 0.072
2.971ValArg: 2.971 ± 0.084
5.461ValSer: 5.461 ± 0.42
5.654ValThr: 5.654 ± 0.135
5.288ValVal: 5.288 ± 0.124
0.652ValTrp: 0.652 ± 0.036
2.534ValTyr: 2.534 ± 0.078
0.0ValXaa: 0.0 ± 0.0
Trp
0.708TrpAla: 0.708 ± 0.04
0.052TrpCys: 0.052 ± 0.01
0.561TrpAsp: 0.561 ± 0.039
0.549TrpGlu: 0.549 ± 0.034
0.4TrpPhe: 0.4 ± 0.03
0.66TrpGly: 0.66 ± 0.041
0.193TrpHis: 0.193 ± 0.019
0.453TrpIle: 0.453 ± 0.031
0.434TrpLys: 0.434 ± 0.027
1.217TrpLeu: 1.217 ± 0.056
0.215TrpMet: 0.215 ± 0.02
0.408TrpAsn: 0.408 ± 0.029
0.251TrpPro: 0.251 ± 0.023
0.636TrpGln: 0.636 ± 0.038
0.459TrpArg: 0.459 ± 0.032
0.595TrpSer: 0.595 ± 0.04
0.613TrpThr: 0.613 ± 0.038
0.706TrpVal: 0.706 ± 0.037
0.119TrpTrp: 0.119 ± 0.016
0.362TrpTyr: 0.362 ± 0.033
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.908TyrAla: 2.908 ± 0.078
0.193TyrCys: 0.193 ± 0.021
2.387TyrAsp: 2.387 ± 0.076
2.007TyrGlu: 2.007 ± 0.068
1.961TyrPhe: 1.961 ± 0.08
2.414TyrGly: 2.414 ± 0.07
0.857TyrHis: 0.857 ± 0.037
1.854TyrIle: 1.854 ± 0.069
1.555TyrLys: 1.555 ± 0.06
4.21TyrLeu: 4.21 ± 0.112
0.694TyrMet: 0.694 ± 0.04
1.476TyrAsn: 1.476 ± 0.069
1.408TyrPro: 1.408 ± 0.051
2.313TyrGln: 2.313 ± 0.069
1.891TyrArg: 1.891 ± 0.06
2.009TyrSer: 2.009 ± 0.069
2.11TyrThr: 2.11 ± 0.067
2.327TyrVal: 2.327 ± 0.068
0.378TyrTrp: 0.378 ± 0.027
1.84TyrTyr: 1.84 ± 0.094
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1598 proteins (497198 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski