Amino acid dipepetide frequency for Salicola phage CGphi29

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.528AlaAla: 9.528 ± 1.741
0.881AlaCys: 0.881 ± 0.227
7.046AlaAsp: 7.046 ± 0.76
8.648AlaGlu: 8.648 ± 0.923
2.402AlaPhe: 2.402 ± 0.45
8.327AlaGly: 8.327 ± 1.239
1.361AlaHis: 1.361 ± 0.334
4.004AlaIle: 4.004 ± 0.558
4.084AlaLys: 4.084 ± 0.771
8.648AlaLeu: 8.648 ± 1.017
2.482AlaMet: 2.482 ± 0.479
3.843AlaAsn: 3.843 ± 0.507
3.363AlaPro: 3.363 ± 0.542
4.404AlaGln: 4.404 ± 0.42
6.326AlaArg: 6.326 ± 0.787
5.445AlaSer: 5.445 ± 0.806
5.525AlaThr: 5.525 ± 0.827
7.527AlaVal: 7.527 ± 0.922
1.842AlaTrp: 1.842 ± 0.475
2.482AlaTyr: 2.482 ± 0.483
0.0AlaXaa: 0.0 ± 0.0
Cys
0.801CysAla: 0.801 ± 0.218
0.24CysCys: 0.24 ± 0.134
1.041CysAsp: 1.041 ± 0.319
0.961CysGlu: 0.961 ± 0.277
0.16CysPhe: 0.16 ± 0.096
0.801CysGly: 0.801 ± 0.288
0.32CysHis: 0.32 ± 0.178
0.641CysIle: 0.641 ± 0.273
0.48CysLys: 0.48 ± 0.25
0.721CysLeu: 0.721 ± 0.218
0.24CysMet: 0.24 ± 0.152
0.08CysAsn: 0.08 ± 0.084
0.641CysPro: 0.641 ± 0.235
0.641CysGln: 0.641 ± 0.261
0.961CysArg: 0.961 ± 0.355
0.24CysSer: 0.24 ± 0.137
0.32CysThr: 0.32 ± 0.156
0.56CysVal: 0.56 ± 0.22
0.16CysTrp: 0.16 ± 0.104
0.4CysTyr: 0.4 ± 0.221
0.0CysXaa: 0.0 ± 0.0
Asp
7.687AspAla: 7.687 ± 0.928
0.961AspCys: 0.961 ± 0.366
4.484AspAsp: 4.484 ± 0.535
5.845AspGlu: 5.845 ± 0.717
2.482AspPhe: 2.482 ± 0.381
6.085AspGly: 6.085 ± 0.72
1.281AspHis: 1.281 ± 0.357
2.722AspIle: 2.722 ± 0.394
2.242AspLys: 2.242 ± 0.542
6.085AspLeu: 6.085 ± 0.625
1.521AspMet: 1.521 ± 0.301
2.082AspAsn: 2.082 ± 0.349
3.123AspPro: 3.123 ± 0.421
2.963AspGln: 2.963 ± 0.499
3.683AspArg: 3.683 ± 0.543
4.564AspSer: 4.564 ± 0.789
4.404AspThr: 4.404 ± 0.629
4.084AspVal: 4.084 ± 0.495
1.842AspTrp: 1.842 ± 0.363
2.562AspTyr: 2.562 ± 0.403
0.0AspXaa: 0.0 ± 0.0
Glu
8.728GluAla: 8.728 ± 0.858
1.121GluCys: 1.121 ± 0.387
3.763GluAsp: 3.763 ± 0.517
5.365GluGlu: 5.365 ± 0.922
2.162GluPhe: 2.162 ± 0.359
6.005GluGly: 6.005 ± 0.714
1.601GluHis: 1.601 ± 0.368
5.365GluIle: 5.365 ± 0.502
3.523GluLys: 3.523 ± 0.378
6.806GluLeu: 6.806 ± 1.046
2.402GluMet: 2.402 ± 0.425
2.642GluAsn: 2.642 ± 0.424
3.683GluPro: 3.683 ± 0.552
4.484GluGln: 4.484 ± 0.901
6.245GluArg: 6.245 ± 0.856
5.044GluSer: 5.044 ± 0.719
3.843GluThr: 3.843 ± 0.494
3.603GluVal: 3.603 ± 0.66
1.922GluTrp: 1.922 ± 0.484
2.082GluTyr: 2.082 ± 0.406
0.0GluXaa: 0.0 ± 0.0
Phe
2.883PheAla: 2.883 ± 0.53
0.4PheCys: 0.4 ± 0.223
3.203PheAsp: 3.203 ± 0.518
1.842PheGlu: 1.842 ± 0.456
0.721PhePhe: 0.721 ± 0.225
2.242PheGly: 2.242 ± 0.396
0.16PheHis: 0.16 ± 0.103
0.641PheIle: 0.641 ± 0.2
1.201PheLys: 1.201 ± 0.311
1.922PheLeu: 1.922 ± 0.415
0.4PheMet: 0.4 ± 0.155
0.721PheAsn: 0.721 ± 0.199
0.881PhePro: 0.881 ± 0.304
0.961PheGln: 0.961 ± 0.216
2.082PheArg: 2.082 ± 0.451
2.162PheSer: 2.162 ± 0.39
1.281PheThr: 1.281 ± 0.322
2.242PheVal: 2.242 ± 0.361
0.4PheTrp: 0.4 ± 0.2
0.721PheTyr: 0.721 ± 0.245
0.0PheXaa: 0.0 ± 0.0
Gly
6.245GlyAla: 6.245 ± 0.708
0.801GlyCys: 0.801 ± 0.292
5.605GlyAsp: 5.605 ± 0.599
6.886GlyGlu: 6.886 ± 0.608
2.162GlyPhe: 2.162 ± 0.435
7.366GlyGly: 7.366 ± 1.123
1.521GlyHis: 1.521 ± 0.366
4.084GlyIle: 4.084 ± 0.541
4.084GlyLys: 4.084 ± 0.553
5.365GlyLeu: 5.365 ± 0.677
2.722GlyMet: 2.722 ± 0.485
4.164GlyAsn: 4.164 ± 0.63
1.842GlyPro: 1.842 ± 0.318
4.004GlyGln: 4.004 ± 0.498
5.125GlyArg: 5.125 ± 0.591
6.326GlySer: 6.326 ± 1.09
3.203GlyThr: 3.203 ± 0.649
6.806GlyVal: 6.806 ± 0.661
1.441GlyTrp: 1.441 ± 0.363
3.443GlyTyr: 3.443 ± 0.601
0.0GlyXaa: 0.0 ± 0.0
His
0.801HisAla: 0.801 ± 0.328
0.56HisCys: 0.56 ± 0.248
1.121HisAsp: 1.121 ± 0.269
1.601HisGlu: 1.601 ± 0.373
0.4HisPhe: 0.4 ± 0.2
1.922HisGly: 1.922 ± 0.447
0.56HisHis: 0.56 ± 0.223
0.641HisIle: 0.641 ± 0.271
0.961HisLys: 0.961 ± 0.345
0.961HisLeu: 0.961 ± 0.282
0.32HisMet: 0.32 ± 0.16
0.56HisAsn: 0.56 ± 0.204
1.521HisPro: 1.521 ± 0.338
0.4HisGln: 0.4 ± 0.172
1.121HisArg: 1.121 ± 0.304
1.121HisSer: 1.121 ± 0.391
0.961HisThr: 0.961 ± 0.331
0.961HisVal: 0.961 ± 0.402
0.48HisTrp: 0.48 ± 0.184
0.641HisTyr: 0.641 ± 0.304
0.0HisXaa: 0.0 ± 0.0
Ile
4.404IleAla: 4.404 ± 0.623
0.801IleCys: 0.801 ± 0.278
4.564IleAsp: 4.564 ± 0.88
4.724IleGlu: 4.724 ± 0.661
1.041IlePhe: 1.041 ± 0.317
4.084IleGly: 4.084 ± 0.641
0.721IleHis: 0.721 ± 0.282
2.002IleIle: 2.002 ± 0.373
2.242IleLys: 2.242 ± 0.442
1.922IleLeu: 1.922 ± 0.298
0.721IleMet: 0.721 ± 0.188
1.922IleAsn: 1.922 ± 0.377
2.322IlePro: 2.322 ± 0.371
1.762IleGln: 1.762 ± 0.415
3.363IleArg: 3.363 ± 0.502
3.043IleSer: 3.043 ± 0.589
4.084IleThr: 4.084 ± 0.588
2.562IleVal: 2.562 ± 0.572
0.721IleTrp: 0.721 ± 0.267
1.201IleTyr: 1.201 ± 0.343
0.0IleXaa: 0.0 ± 0.0
Lys
5.365LysAla: 5.365 ± 0.805
0.32LysCys: 0.32 ± 0.173
2.883LysAsp: 2.883 ± 0.448
3.683LysGlu: 3.683 ± 0.483
1.121LysPhe: 1.121 ± 0.313
4.244LysGly: 4.244 ± 0.706
1.121LysHis: 1.121 ± 0.354
2.242LysIle: 2.242 ± 0.487
2.082LysLys: 2.082 ± 0.517
2.802LysLeu: 2.802 ± 0.514
1.041LysMet: 1.041 ± 0.364
2.162LysAsn: 2.162 ± 0.414
2.963LysPro: 2.963 ± 0.589
1.521LysGln: 1.521 ± 0.376
3.683LysArg: 3.683 ± 0.799
3.203LysSer: 3.203 ± 0.463
3.603LysThr: 3.603 ± 0.616
2.402LysVal: 2.402 ± 0.444
0.641LysTrp: 0.641 ± 0.249
0.961LysTyr: 0.961 ± 0.241
0.0LysXaa: 0.0 ± 0.0
Leu
7.046LeuAla: 7.046 ± 0.947
0.4LeuCys: 0.4 ± 0.191
4.804LeuAsp: 4.804 ± 0.618
5.845LeuGlu: 5.845 ± 0.993
1.762LeuPhe: 1.762 ± 0.41
4.964LeuGly: 4.964 ± 1.498
0.801LeuHis: 0.801 ± 0.257
3.523LeuIle: 3.523 ± 0.57
3.923LeuLys: 3.923 ± 0.772
3.843LeuLeu: 3.843 ± 0.67
2.002LeuMet: 2.002 ± 0.477
2.082LeuAsn: 2.082 ± 0.443
3.043LeuPro: 3.043 ± 0.482
3.043LeuGln: 3.043 ± 0.462
6.005LeuArg: 6.005 ± 0.777
4.084LeuSer: 4.084 ± 0.756
4.724LeuThr: 4.724 ± 0.771
4.324LeuVal: 4.324 ± 0.549
1.121LeuTrp: 1.121 ± 0.305
1.922LeuTyr: 1.922 ± 0.359
0.0LeuXaa: 0.0 ± 0.0
Met
3.523MetAla: 3.523 ± 0.48
0.16MetCys: 0.16 ± 0.13
1.041MetAsp: 1.041 ± 0.318
1.681MetGlu: 1.681 ± 0.317
0.4MetPhe: 0.4 ± 0.189
1.281MetGly: 1.281 ± 0.359
0.4MetHis: 0.4 ± 0.188
1.041MetIle: 1.041 ± 0.252
1.281MetLys: 1.281 ± 0.397
1.201MetLeu: 1.201 ± 0.3
0.961MetMet: 0.961 ± 0.319
0.721MetAsn: 0.721 ± 0.224
1.361MetPro: 1.361 ± 0.347
1.041MetGln: 1.041 ± 0.347
2.322MetArg: 2.322 ± 0.366
2.322MetSer: 2.322 ± 0.397
2.002MetThr: 2.002 ± 0.45
1.281MetVal: 1.281 ± 0.296
0.4MetTrp: 0.4 ± 0.138
0.4MetTyr: 0.4 ± 0.166
0.0MetXaa: 0.0 ± 0.0
Asn
4.404AsnAla: 4.404 ± 0.515
0.32AsnCys: 0.32 ± 0.166
2.883AsnAsp: 2.883 ± 0.299
1.922AsnGlu: 1.922 ± 0.436
0.801AsnPhe: 0.801 ± 0.3
4.484AsnGly: 4.484 ± 0.742
0.641AsnHis: 0.641 ± 0.254
1.601AsnIle: 1.601 ± 0.358
1.922AsnLys: 1.922 ± 0.38
2.562AsnLeu: 2.562 ± 0.411
0.48AsnMet: 0.48 ± 0.18
1.521AsnAsn: 1.521 ± 0.375
2.562AsnPro: 2.562 ± 0.52
2.002AsnGln: 2.002 ± 0.318
1.762AsnArg: 1.762 ± 0.423
2.482AsnSer: 2.482 ± 0.389
2.562AsnThr: 2.562 ± 0.466
2.402AsnVal: 2.402 ± 0.436
0.16AsnTrp: 0.16 ± 0.17
0.56AsnTyr: 0.56 ± 0.196
0.0AsnXaa: 0.0 ± 0.0
Pro
2.722ProAla: 2.722 ± 0.457
0.721ProCys: 0.721 ± 0.272
3.763ProAsp: 3.763 ± 0.711
4.964ProGlu: 4.964 ± 0.548
1.041ProPhe: 1.041 ± 0.321
4.164ProGly: 4.164 ± 0.802
0.801ProHis: 0.801 ± 0.246
1.281ProIle: 1.281 ± 0.243
2.802ProLys: 2.802 ± 0.631
2.162ProLeu: 2.162 ± 0.338
1.361ProMet: 1.361 ± 0.375
2.402ProAsn: 2.402 ± 0.355
2.722ProPro: 2.722 ± 0.587
2.082ProGln: 2.082 ± 0.452
1.762ProArg: 1.762 ± 0.44
2.242ProSer: 2.242 ± 0.446
2.322ProThr: 2.322 ± 0.528
3.123ProVal: 3.123 ± 0.608
0.801ProTrp: 0.801 ± 0.271
1.521ProTyr: 1.521 ± 0.347
0.0ProXaa: 0.0 ± 0.0
Gln
5.205GlnAla: 5.205 ± 0.824
0.32GlnCys: 0.32 ± 0.172
3.043GlnAsp: 3.043 ± 0.512
2.482GlnGlu: 2.482 ± 0.473
1.842GlnPhe: 1.842 ± 0.511
3.283GlnGly: 3.283 ± 0.55
0.961GlnHis: 0.961 ± 0.321
2.082GlnIle: 2.082 ± 0.457
1.762GlnLys: 1.762 ± 0.334
3.443GlnLeu: 3.443 ± 0.63
1.281GlnMet: 1.281 ± 0.316
1.441GlnAsn: 1.441 ± 0.251
2.002GlnPro: 2.002 ± 0.481
2.322GlnGln: 2.322 ± 0.473
2.722GlnArg: 2.722 ± 0.527
2.562GlnSer: 2.562 ± 0.429
3.363GlnThr: 3.363 ± 0.507
1.762GlnVal: 1.762 ± 0.383
1.041GlnTrp: 1.041 ± 0.251
1.521GlnTyr: 1.521 ± 0.396
0.0GlnXaa: 0.0 ± 0.0
Arg
6.886ArgAla: 6.886 ± 0.854
0.32ArgCys: 0.32 ± 0.164
4.404ArgAsp: 4.404 ± 0.442
5.525ArgGlu: 5.525 ± 0.917
1.601ArgPhe: 1.601 ± 0.438
4.404ArgGly: 4.404 ± 0.58
1.281ArgHis: 1.281 ± 0.321
4.324ArgIle: 4.324 ± 0.562
3.923ArgLys: 3.923 ± 0.649
5.205ArgLeu: 5.205 ± 0.777
1.441ArgMet: 1.441 ± 0.371
2.402ArgAsn: 2.402 ± 0.487
2.082ArgPro: 2.082 ± 0.418
3.443ArgGln: 3.443 ± 0.604
3.843ArgArg: 3.843 ± 0.654
4.564ArgSer: 4.564 ± 0.822
2.162ArgThr: 2.162 ± 0.479
3.923ArgVal: 3.923 ± 0.714
1.121ArgTrp: 1.121 ± 0.339
2.322ArgTyr: 2.322 ± 0.466
0.0ArgXaa: 0.0 ± 0.0
Ser
7.607SerAla: 7.607 ± 0.751
0.24SerCys: 0.24 ± 0.143
4.884SerAsp: 4.884 ± 0.742
5.845SerGlu: 5.845 ± 0.677
1.521SerPhe: 1.521 ± 0.336
6.806SerGly: 6.806 ± 0.857
1.201SerHis: 1.201 ± 0.365
2.963SerIle: 2.963 ± 0.844
2.322SerLys: 2.322 ± 0.593
3.443SerLeu: 3.443 ± 1.013
0.881SerMet: 0.881 ± 0.239
2.322SerAsn: 2.322 ± 0.441
2.562SerPro: 2.562 ± 0.463
2.642SerGln: 2.642 ± 0.507
3.523SerArg: 3.523 ± 0.599
3.203SerSer: 3.203 ± 0.625
3.363SerThr: 3.363 ± 0.602
4.324SerVal: 4.324 ± 0.598
0.961SerTrp: 0.961 ± 0.279
1.201SerTyr: 1.201 ± 0.347
0.0SerXaa: 0.0 ± 0.0
Thr
5.445ThrAla: 5.445 ± 0.568
0.48ThrCys: 0.48 ± 0.183
4.244ThrAsp: 4.244 ± 0.661
4.084ThrGlu: 4.084 ± 0.575
2.162ThrPhe: 2.162 ± 0.465
5.605ThrGly: 5.605 ± 0.643
1.041ThrHis: 1.041 ± 0.352
3.523ThrIle: 3.523 ± 0.587
3.363ThrLys: 3.363 ± 0.557
4.084ThrLeu: 4.084 ± 0.613
1.441ThrMet: 1.441 ± 0.291
2.162ThrAsn: 2.162 ± 0.437
3.363ThrPro: 3.363 ± 0.619
2.402ThrGln: 2.402 ± 0.425
3.283ThrArg: 3.283 ± 0.591
2.082ThrSer: 2.082 ± 0.429
2.883ThrThr: 2.883 ± 0.502
3.923ThrVal: 3.923 ± 0.582
0.881ThrTrp: 0.881 ± 0.252
1.762ThrTyr: 1.762 ± 0.361
0.0ThrXaa: 0.0 ± 0.0
Val
5.605ValAla: 5.605 ± 0.922
0.56ValCys: 0.56 ± 0.278
4.164ValAsp: 4.164 ± 0.5
4.804ValGlu: 4.804 ± 0.556
1.842ValPhe: 1.842 ± 0.366
4.404ValGly: 4.404 ± 0.719
0.721ValHis: 0.721 ± 0.251
2.802ValIle: 2.802 ± 0.517
3.283ValLys: 3.283 ± 0.515
4.244ValLeu: 4.244 ± 0.586
1.681ValMet: 1.681 ± 0.407
3.123ValAsn: 3.123 ± 0.449
2.963ValPro: 2.963 ± 0.475
2.082ValGln: 2.082 ± 0.401
4.084ValArg: 4.084 ± 0.438
4.244ValSer: 4.244 ± 0.779
4.404ValThr: 4.404 ± 0.769
4.164ValVal: 4.164 ± 0.558
0.881ValTrp: 0.881 ± 0.307
2.722ValTyr: 2.722 ± 0.694
0.0ValXaa: 0.0 ± 0.0
Trp
1.201TrpAla: 1.201 ± 0.343
0.48TrpCys: 0.48 ± 0.216
1.441TrpAsp: 1.441 ± 0.369
1.441TrpGlu: 1.441 ± 0.45
0.641TrpPhe: 0.641 ± 0.238
0.641TrpGly: 0.641 ± 0.283
0.4TrpHis: 0.4 ± 0.196
0.881TrpIle: 0.881 ± 0.269
0.801TrpLys: 0.801 ± 0.272
1.762TrpLeu: 1.762 ± 0.386
0.56TrpMet: 0.56 ± 0.266
0.641TrpAsn: 0.641 ± 0.231
0.48TrpPro: 0.48 ± 0.211
0.881TrpGln: 0.881 ± 0.33
1.281TrpArg: 1.281 ± 0.281
1.281TrpSer: 1.281 ± 0.345
1.041TrpThr: 1.041 ± 0.346
0.961TrpVal: 0.961 ± 0.285
0.32TrpTrp: 0.32 ± 0.177
0.4TrpTyr: 0.4 ± 0.16
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.082TyrAla: 2.082 ± 0.429
0.32TyrCys: 0.32 ± 0.188
2.322TyrAsp: 2.322 ± 0.364
2.242TyrGlu: 2.242 ± 0.4
0.801TyrPhe: 0.801 ± 0.233
2.082TyrGly: 2.082 ± 0.411
0.721TyrHis: 0.721 ± 0.263
1.842TyrIle: 1.842 ± 0.39
1.762TyrLys: 1.762 ± 0.397
2.082TyrLeu: 2.082 ± 0.412
0.801TyrMet: 0.801 ± 0.23
1.041TyrAsn: 1.041 ± 0.277
1.361TyrPro: 1.361 ± 0.317
1.361TyrGln: 1.361 ± 0.32
2.002TyrArg: 2.002 ± 0.486
1.601TyrSer: 1.601 ± 0.388
2.162TyrThr: 2.162 ± 0.664
1.842TyrVal: 1.842 ± 0.411
0.4TyrTrp: 0.4 ± 0.162
0.56TyrTyr: 0.56 ± 0.216
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 64 proteins (12490 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski