Candidatus Thiodictyon syntrophicum
Average proteome isoelectric point is 6.59
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6068 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2K8UEG2|A0A2K8UEG2_9GAMM Glyco_trans_4-like_N domain-containing protein OS=Candidatus Thiodictyon syntrophicum OX=1166950 GN=THSYN_24880 PE=4 SV=1
MM1 pKa = 7.14 ATLVVDD7 pKa = 4.6 RR8 pKa = 11.84 GLPVANLTTPNLNISAGVNRR28 pKa = 11.84 SNVDD32 pKa = 2.72 WSGYY36 pKa = 5.6 TAGGQSFITGDD47 pKa = 3.81 TFQMPVDD54 pKa = 3.99 QQWTIDD60 pKa = 3.97 TIRR63 pKa = 11.84 TWSPAIPLAGSDD75 pKa = 3.97 TLGSLYY81 pKa = 10.97 SSITLYY87 pKa = 11.3 ADD89 pKa = 4.19 LYY91 pKa = 11.1 SNLGNDD97 pKa = 3.63 PATWAINKK105 pKa = 9.9 AGTISGNTSNNDD117 pKa = 2.66 ITFTRR122 pKa = 11.84 VLYY125 pKa = 10.57 QDD127 pKa = 3.1 ATAPNDD133 pKa = 3.73 DD134 pKa = 3.89 YY135 pKa = 11.54 QGQSGTWSQIWQVDD149 pKa = 4.04 FKK151 pKa = 11.29 NLNWVVTPGDD161 pKa = 3.79 WIVFGVDD168 pKa = 2.87 AAALTTLPWSNHH180 pKa = 4.62 ASNAALSNSPQQDD193 pKa = 2.97 ADD195 pKa = 4.03 DD196 pKa = 4.82 FFTFINMTAGFYY208 pKa = 10.92 GDD210 pKa = 4.81 WYY212 pKa = 10.33 DD213 pKa = 4.74 CDD215 pKa = 3.84 SASDD219 pKa = 4.29 PEE221 pKa = 4.73 CVWGNSSDD229 pKa = 3.61 INVQVFATPAPGSLALALIGLFGLSAARR257 pKa = 11.84 RR258 pKa = 11.84 TEE260 pKa = 3.76 ARR262 pKa = 11.84 STAARR267 pKa = 11.84 CAPP270 pKa = 3.71
Molecular weight: 28.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.707
IPC2_protein 3.783
IPC_protein 3.808
Toseland 3.567
ProMoST 4.012
Dawson 3.834
Bjellqvist 3.986
Wikipedia 3.834
Rodwell 3.63
Grimsley 3.465
Solomon 3.821
Lehninger 3.783
Nozaki 3.948
DTASelect 4.304
Thurlkill 3.643
EMBOSS 3.834
Sillero 3.935
Patrickios 1.926
IPC_peptide 3.821
IPC2_peptide 3.91
IPC2.peptide.svr19 3.837
Protein with the highest isoelectric point:
>tr|A0A2K8UGB6|A0A2K8UGB6_9GAMM ATP synthase subunit beta OS=Candidatus Thiodictyon syntrophicum OX=1166950 GN=atpD PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.56 RR3 pKa = 11.84 TFQPSRR9 pKa = 11.84 IKK11 pKa = 10.5 RR12 pKa = 11.84 ARR14 pKa = 11.84 THH16 pKa = 5.94 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 SATRR25 pKa = 11.84 NGRR28 pKa = 11.84 KK29 pKa = 9.31 VLSARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.62 GRR39 pKa = 11.84 VRR41 pKa = 11.84 LAPP44 pKa = 3.89
Molecular weight: 5.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.286
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.676
Grimsley 13.086
Solomon 13.539
Lehninger 13.437
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.398
IPC_peptide 13.539
IPC2_peptide 12.53
IPC2.peptide.svr19 9.215
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6068
0
6068
2008834
27
3891
331.1
36.04
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.777 ± 0.053
1.069 ± 0.013
5.858 ± 0.025
5.429 ± 0.033
3.301 ± 0.018
8.547 ± 0.038
2.11 ± 0.017
4.235 ± 0.022
2.429 ± 0.023
11.581 ± 0.044
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.818 ± 0.014
2.312 ± 0.018
5.952 ± 0.028
3.744 ± 0.019
8.138 ± 0.035
4.639 ± 0.022
5.234 ± 0.032
6.935 ± 0.028
1.536 ± 0.015
2.358 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here