Motilibacter rhizosphaerae

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Motilibacterales; Motilibacteraceae; Motilibacter

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3963 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Q7NU06|A0A4Q7NU06_9ACTN 50S ribosomal protein L14 OS=Motilibacter rhizosphaerae OX=598652 GN=rplN PE=3 SV=1
MM1 pKa = 7.01FVNWPVTDD9 pKa = 4.35LDD11 pKa = 3.88RR12 pKa = 11.84AKK14 pKa = 10.93AFYY17 pKa = 9.31EE18 pKa = 3.95ALGFTVNPAFTDD30 pKa = 3.44HH31 pKa = 6.79NAACVVVEE39 pKa = 4.18EE40 pKa = 4.16DD41 pKa = 2.99HH42 pKa = 7.18SYY44 pKa = 11.71FMLLVRR50 pKa = 11.84EE51 pKa = 4.65FFQTFTDD58 pKa = 4.76LPIGDD63 pKa = 4.57PAVSPSVAVAVFLDD77 pKa = 3.41SRR79 pKa = 11.84EE80 pKa = 4.16AVDD83 pKa = 3.4AAVAAGLAAGGSEE96 pKa = 4.02GHH98 pKa = 6.95PAADD102 pKa = 3.43YY103 pKa = 10.94GFMYY107 pKa = 10.01QRR109 pKa = 11.84QLADD113 pKa = 3.3PDD115 pKa = 4.29GNVLEE120 pKa = 4.99FGWMDD125 pKa = 3.69PVAAQQGPEE134 pKa = 4.39AYY136 pKa = 9.81AQQQAA141 pKa = 3.41

Molecular weight: 15.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Q7NAK6|A0A4Q7NAK6_9ACTN GAF domain-containing protein OS=Motilibacter rhizosphaerae OX=598652 GN=EV189_3440 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.7LLRR22 pKa = 11.84KK23 pKa = 7.97TRR25 pKa = 11.84VQRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.81KK32 pKa = 9.63

Molecular weight: 4.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3963

0

3963

1320903

29

3232

333.3

35.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.784 ± 0.065

0.688 ± 0.01

5.807 ± 0.033

5.393 ± 0.05

2.357 ± 0.024

9.661 ± 0.037

1.997 ± 0.017

2.353 ± 0.029

1.548 ± 0.028

11.132 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.349 ± 0.014

1.306 ± 0.02

6.092 ± 0.034

2.824 ± 0.024

8.072 ± 0.051

5.413 ± 0.04

5.838 ± 0.049

10.041 ± 0.042

1.506 ± 0.018

1.839 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski